scholarly journals AB0095 By intravenous infusion marked by green fluorescent protein to reveal bone marrow mesenchymal stem cells' distribution and differentiation of collagen induced arthritis rats

2017 ◽  
Author(s):  
J Gao ◽  
L Zhang
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Green Fluorescent Protein ◽  
Intravenous Infusion ◽  
Fluorescent Protein ◽  
Collagen Induced Arthritis ◽  
Marrow Mesenchymal Stem Cells ◽  
Green Fluorescent

Meniscal repair using bone marrow-derived mesenchymal stem cells: experimental study using green fluorescent protein transgenic rats

The Knee ◽  
2005 ◽  
Vol 12 (3) ◽  
pp. 217-223 ◽  
Author(s):  
Yasunori Izuta ◽  
Mitsuo Ochi ◽  
Nobuo Adachi ◽  
Masataka Deie ◽  
Takuma Yamasaki ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Experimental Study ◽  
Mesenchymal Stem Cells ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Meniscal Repair ◽  
Transgenic Rats ◽  
Green Fluorescent

Retrovirally mediated correction of bone marrow–derived mesenchymal stem cells from patients with mucopolysaccharidosis type I

Blood ◽  
2002 ◽  
Vol 99 (5) ◽  
pp. 1857-1859 ◽  
Author(s):  
Melissa A. Baxter ◽  
Robert F. Wynn ◽  
Jonathan A. Deakin ◽  
Ilaria Bellantuono ◽  
Kirsten G. Edington ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Dermatan Sulfate ◽  
Type I ◽  
Mucopolysaccharidosis Type ◽  
Extracellular Medium ◽  
Green Fluorescent

We have investigated the utility of bone marrow–derived mesenchymal stem cells (MSCs) as targets for gene therapy of the autosomal recessive disorder mucopolysaccharidosis type IH (MPS-IH, Hurler syndrome). Cultures of MSCs were initially exposed to a green fluorescent protein–expressing retrovirus. Green fluorescent protein–positive cells maintained their proliferative and differentiation capacity. Next we used a vector encoding α-l-iduronidase (IDUA), the enzyme that is defective in MPS-IH. Following transduction, MPS-IH MSCs expressed high levels of IDUA and secreted supernormal levels of this enzyme into the extracellular medium. Exogenous IDUA expression led to a normalization of glycosaminoglycan storage in MPS-IH cells, as evidenced by a dramatic decrease in the amount of 35SO4sequestered within the heparan sulfate and dermatan sulfate compartments of these cells. Finally, gene-modified MSCs were able to cross-correct the enzyme defect in untransduced MPS-IH fibroblasts via protein transfer.

scholarly journals AB1011 Clinical trial of intravenous infusion of fucosylated bone marrow mesenchymal stem cells in patients with osteoporosis

2018 ◽  
Author(s):  
N. Lozano-Rivas ◽  
L.F. Linares ◽  
C. Marras-Fernandez-Cid ◽  
A.M. Garcia-Hernandez ◽  
M.D.C. Algueró ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Intravenous Infusion ◽  
Marrow Mesenchymal Stem Cells

scholarly journals Hypoxic preconditioning enhances bone marrow mesenchymal stem cell migration via Kv2.1 channel and FAK activation

2011 ◽  
Vol 301 (2) ◽  
pp. C362-C372 ◽  
Author(s):  
Xinyang Hu ◽  
Ling Wei ◽  
Tammi M. Taylor ◽  
Jianfeng Wei ◽  
Xin Zhou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Stem Cell ◽  
Fluorescent Protein ◽  
Hypoxic Preconditioning ◽  
Delayed Rectifier ◽  
Marrow Mesenchymal Stem Cells ◽  
Injured Region ◽  
Green Fluorescent

Transplantation using stem cells including bone marrow mesenchymal stem cells (BMSCs) is emerging as a potential regenerative therapy after ischemic attacks in the heart and brain. The migration capability of transplanted cells is a critical cellular function for tissue repair. Based on our recent observations that hypoxic preconditioning (HP) has multiple benefits in improving stem cell therapy and that the potassium Kv2.1 channel acts as a promoter for focal adhesion kinase (FAK) activation and cell motility, the present investigation tested the hypothesis that HP treatment can increase BMSC migration via the mechanism of increased Kv2.1 expression and FAK activities. BMSCs derived from green fluorescent protein-transgenic mice were treated under either normoxic (N-BMSC) or hypoxic (0.5% O2) (HP-BMSC) conditions for 24 h. Western blot analysis showed HP selectively upregulated Kv2.1 expression while leaving other K+ channels, such as Kv1.5 and Kv1.4, unaffected. Compared with normoxic controls, significantly larger outward delayed rectifier K+ currents were recorded in HP-BMSCs. HP enhanced BMSC migration/homing activities in vitro and after intravenous transplantation into rats subjected to permanent myocardial infarction (MI). The HP-promoted BMSC migration was inhibited by either blocking K+ channels or knocking down Kv2.1. Supporting a relationship among HP, Kv2.1, and FAK activation, HP increased phosphorylation of FAK397 and FAK576/577, and this effect was antagonized by blocking K+ channels. These findings provide novel evidence that HP enhances the ability of BMSCs to migrate and home to the injured region; this effect is mediated through a regulatory role of Kv2.1 on FAK phosphorylation/activation.

scholarly journals Cell therapy using allogeneic bone marrow mesenchymal stem cells prevents tissue damage in collagen-induced arthritis

2007 ◽  
Vol 56 (4) ◽  
pp. 1175-1186 ◽  
Author(s):  
Andrea Augello ◽  
Roberta Tasso ◽  
Simone Maria Negrini ◽  
Ranieri Cancedda ◽  
Giuseppina Pennesi
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cell Therapy ◽  
Tissue Damage ◽  
Allogeneic Bone Marrow ◽  
Allogeneic Bone ◽  
Collagen Induced Arthritis ◽  
Marrow Mesenchymal Stem Cells

scholarly journals Hematopoietic stem cells can differentiate into pericytes and myofibroblast in wound healing, not bone Mesenchymal stem cells

2020 ◽  
Author(s):  
Yanan Kong ◽  
Liuhanghang Cheng ◽  
Min Xuan ◽  
Hao Ding ◽  
Biao Cheng
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Fluorescent Protein ◽  
Bone Mesenchymal Stem Cells ◽  
Hematopoietic Stem ◽  
Green Fluorescent

Abstract Background Hematopoietic stem cells(HSCs) and mesenchymal stem cells(MSCs) can participate in wound healing. However, very few studies had shown HSCs and MSCs could arrive to the wound and differentiate into tissues. In this study, we intend to investigate the role of bone marrow HSCs and MSCs in wound healing. Methods We first removed the bone marrow of mice by irradiation. Furthermore, we injected different colours of fluorescent HSCs and MSCs into the tail vein of irradiated mice to reconstruct bone marrow function. We prepared wound models on the back of these mice. In vivo imaging and immunohistochemical staining were used to track the expression of fluorescent protein. Results HSCs and MSCs have been isolated and cultured. HSCs expressed expressed Sca1, not lineage, CD34 or CD48. MSCs expressed expressed CD29 and CD44,not CD34 or CD45. HSCs labeled with green fluorescent protein reached the wound and co-expressed with desmin and α-SMA. MSCs didn’t stay on the wound. Conclusions The results show HSCs in the bone marrow of mice can directly participate in wound healing and differentiate into pericytes and myofibroblasts.

Sign in / Sign up

Export Citation Format

Share Document