AB0094 Migration, colonization and distribution of bone marrow mesenchymal stem cells transplanted in cia rats were traced by green fluorescent protein

We have investigated the utility of bone marrow–derived mesenchymal stem cells (MSCs) as targets for gene therapy of the autosomal recessive disorder mucopolysaccharidosis type IH (MPS-IH, Hurler syndrome). Cultures of MSCs were initially exposed to a green fluorescent protein–expressing retrovirus. Green fluorescent protein–positive cells maintained their proliferative and differentiation capacity. Next we used a vector encoding α-l-iduronidase (IDUA), the enzyme that is defective in MPS-IH. Following transduction, MPS-IH MSCs expressed high levels of IDUA and secreted supernormal levels of this enzyme into the extracellular medium. Exogenous IDUA expression led to a normalization of glycosaminoglycan storage in MPS-IH cells, as evidenced by a dramatic decrease in the amount of 35SO4sequestered within the heparan sulfate and dermatan sulfate compartments of these cells. Finally, gene-modified MSCs were able to cross-correct the enzyme defect in untransduced MPS-IH fibroblasts via protein transfer.

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Effect of targeted ovarian cancer therapy using amniotic fluid mesenchymal stem cells transfected with enhanced green fluorescent protein-human interleukin-2 in vivo

Molecular Medicine Reports ◽

10.3892/mmr.2015.4076 ◽

2015 ◽

Vol 12 (4) ◽

pp. 4859-4866 ◽

Cited By ~ 9

Author(s):

QI YOU ◽

YUAN YAO ◽

YUANLONG ZHANG ◽

SONGBIN FU ◽

MEI DU ◽

...

Keyword(s):

Ovarian Cancer ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Green Fluorescent Protein ◽

Cancer Therapy ◽

Enhanced Green Fluorescent Protein ◽

Fluorescent Protein ◽

Interleukin 2 ◽

Green Fluorescent

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Hypoxic preconditioning enhances bone marrow mesenchymal stem cell migration via Kv2.1 channel and FAK activation

AJP Cell Physiology ◽

10.1152/ajpcell.00013.2010 ◽

2011 ◽

Vol 301 (2) ◽

pp. C362-C372 ◽

Cited By ~ 81

Author(s):

Xinyang Hu ◽

Ling Wei ◽

Tammi M. Taylor ◽

Jianfeng Wei ◽

Xin Zhou ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Stem Cell ◽

Fluorescent Protein ◽

Hypoxic Preconditioning ◽

Delayed Rectifier ◽

Marrow Mesenchymal Stem Cells ◽

Injured Region ◽

Green Fluorescent

Transplantation using stem cells including bone marrow mesenchymal stem cells (BMSCs) is emerging as a potential regenerative therapy after ischemic attacks in the heart and brain. The migration capability of transplanted cells is a critical cellular function for tissue repair. Based on our recent observations that hypoxic preconditioning (HP) has multiple benefits in improving stem cell therapy and that the potassium Kv2.1 channel acts as a promoter for focal adhesion kinase (FAK) activation and cell motility, the present investigation tested the hypothesis that HP treatment can increase BMSC migration via the mechanism of increased Kv2.1 expression and FAK activities. BMSCs derived from green fluorescent protein-transgenic mice were treated under either normoxic (N-BMSC) or hypoxic (0.5% O2) (HP-BMSC) conditions for 24 h. Western blot analysis showed HP selectively upregulated Kv2.1 expression while leaving other K+ channels, such as Kv1.5 and Kv1.4, unaffected. Compared with normoxic controls, significantly larger outward delayed rectifier K+ currents were recorded in HP-BMSCs. HP enhanced BMSC migration/homing activities in vitro and after intravenous transplantation into rats subjected to permanent myocardial infarction (MI). The HP-promoted BMSC migration was inhibited by either blocking K+ channels or knocking down Kv2.1. Supporting a relationship among HP, Kv2.1, and FAK activation, HP increased phosphorylation of FAK397 and FAK576/577, and this effect was antagonized by blocking K+ channels. These findings provide novel evidence that HP enhances the ability of BMSCs to migrate and home to the injured region; this effect is mediated through a regulatory role of Kv2.1 on FAK phosphorylation/activation.

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Hematopoietic stem cells can differentiate into pericytes and myofibroblast in wound healing, not bone Mesenchymal stem cells

10.21203/rs.3.rs-81020/v1 ◽

2020 ◽

Author(s):

Yanan Kong ◽

Liuhanghang Cheng ◽

Min Xuan ◽

Hao Ding ◽

Biao Cheng

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Wound Healing ◽

Mesenchymal Stem Cells ◽

Hematopoietic Stem Cells ◽

Fluorescent Protein ◽

Bone Mesenchymal Stem Cells ◽

Hematopoietic Stem ◽

Green Fluorescent

Abstract Background Hematopoietic stem cells(HSCs) and mesenchymal stem cells(MSCs) can participate in wound healing. However, very few studies had shown HSCs and MSCs could arrive to the wound and differentiate into tissues. In this study, we intend to investigate the role of bone marrow HSCs and MSCs in wound healing. Methods We first removed the bone marrow of mice by irradiation. Furthermore, we injected different colours of fluorescent HSCs and MSCs into the tail vein of irradiated mice to reconstruct bone marrow function. We prepared wound models on the back of these mice. In vivo imaging and immunohistochemical staining were used to track the expression of fluorescent protein. Results HSCs and MSCs have been isolated and cultured. HSCs expressed expressed Sca1, not lineage, CD34 or CD48. MSCs expressed expressed CD29 and CD44,not CD34 or CD45. HSCs labeled with green fluorescent protein reached the wound and co-expressed with desmin and α-SMA. MSCs didn’t stay on the wound. Conclusions The results show HSCs in the bone marrow of mice can directly participate in wound healing and differentiate into pericytes and myofibroblasts.

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Bone Marrow Mesenchymal Stem Cells (BMSCs)-Derived miR-31 in the Pathogenesis of Recurrent Abortion by Regulating Kisspeptins 1 (KISS1) Expression

Journal of Biomaterials and Tissue Engineering ◽

10.1166/jbt.2021.2785 ◽

2021 ◽

Vol 11 (11) ◽

pp. 2232-2238

Author(s):

Ruiping Yu ◽

Changfen Wu ◽

Ying Qin ◽

Cong Li ◽

Zhongfu Mo ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Functional Recovery ◽

Pregnancy Loss ◽

Recurrent Pregnancy Loss ◽

Fluorescent Protein ◽

Endometrial Stromal Cells ◽

Aberrant Expression ◽

Marrow Mesenchymal Stem Cells

Bone marrow mesenchymal stem cells (BMSCs) are potentially efficacious in treating recurrent pregnancy disorders and endometrial injury. Uterine parenchymal cells interact with BMSCs to promote functional recovery. Our research aimed to explore the effect of BMSCs-derived miR-31 on recurrent pregnancy loss. A recurrent pregnancy loss mouse model was constructed followed by nanoparticle analysis of BMSC and miR-31 expressing by RT-PCR. The levels of miR-31 in BMSCs (miR-31+BMSCs or BMSCs) and their counterpart exosomes were up- or down-regulated to explore the effects of aberrant expression of miR-31 on endometrial damage in recurrent pregnancy loss. The analysis of BMSC nanoparticles showed that miR-31 was derived from BMSC. We found increased levels of miR-31 in miR-340 + BMSCs after incubation with endometrial stromal cells (ESCs) compared to controls. Labeling of exosomes by red fluorescent protein indicated that exosomes were liberated out of BMSCs and translocated into neighboring ESCs, and mice treated with miR-340 + BMSCs had improved functional recovery from recurrent pregnancy loss. BMSC-derived miR-31 mediates functional recovery induced in recurrent pregnancy miscarriage mice by regulating KISS1 expression and fibrosis gene expression.

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Survival of bone marrow mesenchymal stem cells labelled with red fluorescent protein in an ovine model of collagenase-induced tendinitis

Veterinary and Comparative Orthopaedics and Traumatology ◽

10.3415/vcot-13-09-0113 ◽

2014 ◽

Vol 27 (03) ◽

pp. 204-209 ◽

Cited By ~ 19

Author(s):

F. Staffieri ◽

G. Rossi ◽

E. Francioso ◽

A. Crovace ◽

L. Lacitignola

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Fibrin Glue ◽

Fluorescent Protein ◽

Allogeneic Bone ◽

Red Fluorescent Protein ◽

Ovine Model ◽

Cd34 Cells ◽

Marrow Mesenchymal Stem Cells

SummaryObjective: The aim of this study was to track the survival and efficacy of allogeneic bone marrow mesenchymal stem cells (BM-MSC) marked with red fluorescent protein (BMMSCRFP) in an ovine model of collagenase-induced tendinopathy.Methods: Bone marrow was harvested from one donor sheep and BM-MSC were isolated, cultivated and transfected with red fluorescent protein (BM-MSCRFP). Collagenase was injected into both Achilles tendons in the remaining nine sheep. After two weeks the left tendon was injected with a solution of 6 x 106 BM-MSCRFP and fibrin glue, while only fibrin glue was administered to the contralateral tendon in each sheep. After three, four and six weeks the tendons were harvested and evaluated for morphology, collagen I deposition, presence of CD34+ cells, and fluorescent labelled BM-MSC.Results: We demonstrated that delivery of BM-MSC into tendon lesions had positive effects on the injured tendons. The BM-MSCRFP survived at three, four and six weeks after treatment, leading to better quality healing of tendons as compared to the controls, where no labelled cells were detected. Interestingly, we demonstrated high expression of CD34+ cells in tendons that had been treated with BM-MSCRFP.Clinical relevance: Mesenchymal stem cell allografts have a positive effect on tendon healing and local injection of BM-MSC directly into the tendon allows the homing of BM-MSC for good efficiency of engraftment.

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