scholarly journals The validity of capillary blood sampling in the determination of human growth hormone concentration during exercise in men

2004 ◽  
Vol 38 (5) ◽  
pp. e27-e27 ◽  
Author(s):  
R J Godfrey
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Human Growth ◽  
Capillary Blood ◽  
Blood Sampling ◽  
Hormone Concentration ◽  
Growth Hormone Concentration ◽  
Capillary Blood Sampling

INCREASE IN PLASMA GROWTH HORMONE CONCENTRATION IN MAN AFTER INFUSION OF ADRENALINE-PROPRANOLOL

1970 ◽  
Vol 47 (1) ◽  
pp. 95-100 ◽  
Author(s):  
F. MASSARA ◽  
E. STRUMIA
Keyword(s):  
Growth Hormone ◽  
Healthy Volunteers ◽  
Human Growth Hormone ◽  
Human Growth ◽  
Hormone Concentration ◽  
Nacl Solution ◽  
Plasma Growth Hormone ◽  
Growth Hormone Concentration ◽  
Wide Range

SUMMARY Plasma human growth hormone (HGH) concentrations were compared during four tests in which 0·9% NaCl solution only, propranolol, adrenaline or propranolol plus adrenaline were infused into six healthy volunteers. In five subjects, HGH levels during the infusion of saline, propranolol or adrenaline showed no significant increase and were virtually identical. During the test with propranolol plus adrenaline, however, there was an increase after adrenaline in spite of concomitant hyperglycaemia. The sixth volunteer showed a wide range of apparently spontaneous and irregular variations in HGH values throughout each of the four tests. These findings support the view that α-receptors stimulate and β-receptors depress HGH secretion, and show that adrenergically stimulated HGH secretion in man is not inhibited by hyperglycaemia.

scholarly journals ELISA for Determination of Human Growth Hormone: Recognition of Helix 4 Epitopes

2004 ◽  
Vol 2004 (3) ◽  
pp. 143-149 ◽  
Author(s):  
Juliana F. Moura ◽  
Luiz DeLacerda ◽  
Romolo Sandrini ◽  
Fernanda M. Borba ◽  
Denise N. Castelo ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Synthetic Peptide ◽  
Human Growth ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunoaffinity Column ◽  
Receptor Dimerization ◽  
Human Prolactin

Human growth hormone (hGH) signal transduction initiates with a receptor dimerization in which one molecule binds to the receptor through sites 1 and 2. A sandwich enzyme-linked immunosorbent assay was developed for quantifying hGH molecules that present helix 4 from binding site 1. For this, horse anti-rhGH antibodies were eluted by an immunoaffinity column constituted by sepharose-rhGH. These antibodies were purified through a second column with synthetic peptide correspondent tohGH helix 4, immobilized to sepharose, and used as capture antibodies. Those that did not recognize synthetic peptide were used as a marker antibody. The working range was of 1.95 to 31.25 ng/mL of hGH. The intra-assay coefficient of variation (CV) was between 4.53% and 6.33%, while the interassay CV was between 6.00% and 8.27%. The recovery range was between 96.0% to 103.8%. There was no cross-reactivity with human prolactin. These features show that our assay is an efficient method for the determination of hGH.

Effect of various blood sampling procedures on serum levels of immunoreactive human growth hormone

Metabolism ◽  
1967 ◽  
Vol 16 (5) ◽  
pp. 402-409 ◽  
Author(s):  
Georges Copinschi ◽  
Martin Hartog ◽  
Jerry M. Earll ◽  
Richard J. Havel
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Human Growth ◽  
Serum Levels ◽  
Blood Sampling ◽  
Sampling Procedures

Multiplexed determination of human growth hormone and prolactin at a label free electrochemical immunosensor using dual carbon nanotube–screen printed electrodes modified with gold and PEDOT nanoparticles

The Analyst ◽  
2014 ◽  
Vol 139 (18) ◽  
pp. 4556-4563 ◽  
Author(s):  
V. Serafín ◽  
G. Martínez-García ◽  
L. Agüí ◽  
P. Yáñez-Sedeño ◽  
J. M. Pingarrón
Keyword(s):  
Growth Hormone ◽  
Carbon Nanotube ◽  
Simultaneous Determination ◽  
Human Growth Hormone ◽  
Human Growth ◽  
Electrochemical Immunosensor ◽  
Label Free ◽  
Screen Printed Electrodes ◽  
First Time

A label-free dual electrochemical immunosensor for simultaneous determination of human growth and prolactin hormones was prepared for the first time.

scholarly journals Agreement between fingertip-capillary and antecubital-venous appetite-related peptides

2014 ◽  
Vol 3 (4) ◽  
pp. 233-242 ◽  
Author(s):  
Benjamin Paul Green ◽  
Javier Thomas Gonzalez ◽  
Kevin Thomas ◽  
Emma Stevenson ◽  
Penny Louise Sheena Rumbold
Keyword(s):  
Venous Blood ◽  
Capillary Blood ◽  
Blood Sampling ◽  
Systematic Bias ◽  
Deming Regression ◽  
Methodological Approaches ◽  
Capillary Blood Sampling ◽  
Venous Blood Sampling ◽  
Good Agreement

This study examined the agreement between fingertip-capillary and antecubital-venous measures of appetite-related peptides. Simultaneous fingertip-capillary and antecubital-venous blood samples were collected from 19 participants. The samples were obtained at baseline, 30, 60, 90, and 120 min following breakfast for the determination of plasma GLP17–36, glucagon, insulin and leptin. Between-day reproducibility of fingertip-capillary-derived estimates was assessed in 18 participants. Deming regression, limits of agreement (LOA) and typical error as a coefficient of variation (CV) were used to quantify agreement (CVa) and reproducibility (CVr). Deming regression revealed no systematic bias for any of the analytes studied, but for insulin there was evidence of a proportional difference at higher concentrations. Measures of GLP17–36 (CVa=24.0%, LOA ±2.5 pg m/l per h), leptin (CVa=9.0%, LOA ×/÷1.19) and glucagon (CVa=21.0%, LOA, ±31.5 pg m/l per h) revealed good agreement between methodological approaches. Fingertip-capillary glucagon was highly reproducible between days (CVr=8.2%). GLP17–36 and leptin demonstrated modest reproducibility (CVr=22.7 and 25.0% respectively). For insulin, agreement (CVa=36.0%, LOA ×/÷1.79) and reproducibility were poor (CVr=36.0%). Collectively, the data demonstrate that fingertip-capillary blood sampling provides a comparable and reproducible alternative to antecubital-venous blood sampling for the quantification of glucagon, and to a lesser extent for GLP17–36 and leptin. Caution should be exercised when utilising fingertip-capillary blood sampling for insulin quantification, and consequently should not be employed interchangeably with antecubital-venous blood sampling.

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