1272 Effects of metal-rich particulate matter exposure on epstein-barr virus and human endogenous retrovirus w (herv-w) methylation healthy steel-workers

ABSTRACT Superantigens are microbial proteins that strongly stimulate T cells. We described previously that the Epstein-Barr virus (EBV) transactivates a superantigen encoded by the human endogenous retrovirus, HERV-K18. We now report that the transactivation is dependent upon the EBV latent cycle proteins. Moreover, LMP-2A is sufficient for induction of HERV-K18 superantigen activity.

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HLA DR2b-binding peptides from human endogenous retrovirus envelope, Epstein-Barr virus and brain proteins in the context of molecular mimicry in multiple sclerosis

Immunology Letters ◽

10.1016/j.imlet.2019.10.017 ◽

2020 ◽

Vol 217 ◽

pp. 15-24 ◽

Cited By ~ 4

Author(s):

Ranjan Ramasamy ◽

Fiyaz Mohammed ◽

Ute-C. Meier

Keyword(s):

Multiple Sclerosis ◽

Epstein Barr Virus ◽

Molecular Mimicry ◽

Endogenous Retrovirus ◽

Human Endogenous Retrovirus ◽

Brain Proteins ◽

Barr Virus ◽

Binding Peptides ◽

Epstein Barr

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METAL-RICH PARTICULATE MATTER INFLUENCE EPSTEIN-BARR VIRUS METHYLATION IN PERIPHERAL BLOOD LEUKOCYTES.

ISEE Conference Abstracts ◽

10.1289/isee.2011.00655 ◽

2011 ◽

Vol 2011 (1) ◽

Author(s):

Valentina Bollati ◽

Laura Angelici ◽

Matteo Bonzini ◽

Letizia Tarantini ◽

Pietro Apostoli ◽

...

Keyword(s):

Particulate Matter ◽

Peripheral Blood ◽

Epstein Barr Virus ◽

Peripheral Blood Leukocytes ◽

Blood Leukocytes ◽

Barr Virus ◽

Epstein Barr

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Human endogenous retrovirus-K18 Env as a risk factor in multiple sclerosis

Multiple Sclerosis Journal ◽

10.1177/1352458508094641 ◽

2008 ◽

Vol 14 (9) ◽

pp. 1175-1180 ◽

Cited By ~ 45

Author(s):

AK Tai ◽

EJ O’Reilly ◽

KA Alroy ◽

KC Simon ◽

KL Munger ◽

...

Keyword(s):

Multiple Sclerosis ◽

Risk Factor ◽

Statistical Significance ◽

Endogenous Retrovirus ◽

Human Endogenous Retrovirus ◽

Barr Virus ◽

Relative Risks ◽

Increased Risk ◽

Epstein Barr ◽

Nested Case Control

Background The human endogenous retrovirus (HERV)-K18 Env is an Epstein-Barr virus (EBV)-associated superantigen. Given the evidence for a role of EBV in the etiology of multiple sclerosis (MS), HERV-K18 Env is a plausible candidate for association with MS. Objective To assess whether variation in HERV-K18 Env is a risk factor for MS. Methods We developed a single nucleotide polymorphism-based genotyping method to determine the distribution of the three alleles of HERV-K18 env. We then conducted a nested case-control study including 207 MS cases and 403 matched controls. Analyses were replicated in an independent series of 909 MS cases and 339 controls. Results Overall, there was a significant association between HERV-K18 env genotype and MS risk (χ2 P = 0.03). As compared with K18.2/K18.2 individuals, risk of MS was three fold higher among K18.3/K18.3 individuals ( P = 0.03). An increase in MS risk among carriers of the K18.3 allele was also observed in the replication study, but did not reach statistical significance. In pooled analyses, K18.3/K18.3 individuals had a significantly increased risk of MS (relative risks [RR] comparing K18.3/K18.3 vs K18.2/K18.2 = 2.7; 95% confidence interval: 1.1–6.4). Conclusion Variation in EBV-associated superantigen HERV-K18 Env could influence the genetic susceptibility to MS.

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The role of infections in multiple sclerosis

Multiple Sclerosis Journal ◽

10.1177/1352458518823940 ◽

2019 ◽

Vol 25 (7) ◽

pp. 891-901 ◽

Cited By ~ 18

Author(s):

Mariano Marrodan ◽

Lucas Alessandro ◽

Mauricio F Farez ◽

Jorge Correale

Keyword(s):

Multiple Sclerosis ◽

Chlamydia Pneumoniae ◽

Treatment Strategies ◽

Endogenous Retrovirus ◽

Human Endogenous Retrovirus ◽

Barr Virus ◽

Microbial Infections ◽

Epstein Barr ◽

New Treatment ◽

Tract Infections

Several lines of evidence suggest that multiple sclerosis (MS), like other autoimmune diseases, may be triggered by microbial infections. Pathogens associated with development or exacerbation of MS include bacteria, such as Chlamydia pneumoniae, Staphylococcus aureus-produced enterotoxins that function as superantigens, and viruses of the Herpesviridae (Epstein–Barr virus and human herpes virus 6) and human endogenous retrovirus families. However, to date, no single pathogen has been accepted as causal agent. In addition, common upper respiratory, gastrointestinal, and urogenital tract infections have also been associated with MS exacerbations. Although evidence of an infectious etiology as cause of MS in humans remains inconclusive, microbial agents may modulate the neuroimmunological system of genetically susceptible individuals. Decoding the epidemiological contribution of different microorganisms to MS, along with their pathogenic mechanisms, may help develop new treatment strategies and prevent relapses.

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Anti-Human Herpesvirus 6 A/B Antibodies Titers Correlate With Multiple Sclerosis-Associated Retrovirus Envelope Expression

Frontiers in Immunology ◽

10.3389/fimmu.2021.798003 ◽

2021 ◽

Vol 12 ◽

Author(s):

Silvia Pérez-Pérez ◽

María I. Domínguez-Mozo ◽

M. Ángel García-Martínez ◽

M. Celeste García-Frontini ◽

Noelia Villarrubia ◽

...

Keyword(s):

Gene Expression ◽

Multiple Sclerosis ◽

Human Herpesvirus ◽

Endogenous Retrovirus ◽

Human Endogenous Retrovirus ◽

Expression Levels ◽

Barr Virus ◽

Healthy Donors ◽

Epstein Barr ◽

Gene Expression Levels

Human endogenous retrovirus W family envelope proteins (pHERV-W ENV/syncytin-1) have been repeatedly associated with multiple sclerosis (MS). Here, we have focused on the study of pHERV-W ENV/syncytin-1 expression levels in MS patients (relapsing and progressive forms) and in healthy donors (HD) and on exploring their possible relationship with Epstein-Barr virus (EBV) and human herpesvirus-6A/B (HHV-6A/B). We included blood samples from 101 MS patients and 37 HD to analyze antiviral antibody titers by ELISA and pHERV-W ENV/syncytin-1 expression levels by flow cytometry as well as by qPCR. Patients with relapsing MS forms showed significantly higher pHERV-W ENV/syncytin-1 protein and gene expression levels than HD. Progressive MS patients also showed significantly higher protein and gene expression levels than both HD and relapsing MS patients. Regarding antiviral antibodies titers, anti-HHV-6A/B IgM levels were positively correlated with pHERV-W ENV/syncytin-1 protein expression levels in patients with relapsing MS, while in the progressive forms patients this correlation was found with anti-HHVA/B IgG levels. Therefore, pHERV-W ENV could be involved in MS pathogenesis, playing a role in relapsing and progressive forms. Besides, anti-HHV-6A/B antibodies positively correlated with pHERV-W ENV expression. Further studies are needed to better understand this possible relationship.

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A Quantitative Ultrastructural Study of Peripheral Blood Lymphocytes Containing Parallel Tubular Arrays in Epstein-Barr Virus and Cytomegalo-Virus Mononucleosis

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100098769 ◽

1981 ◽

Vol 39 ◽

pp. 454-455

Author(s):

C. M. Payne ◽

P. M. Tennican

Keyword(s):

Peripheral Blood ◽

Lymphocyte Count ◽

Epstein Barr Virus ◽

Absolute Lymphocyte Count ◽

Peripheral Blood Lymphocytes ◽

Clinical State ◽

Barr Virus ◽

The Absolute ◽

Epstein Barr ◽

Tubular Arrays

In the normal peripheral circulation there exists a sub-population of lymphocytes which is ultrastructurally distinct. This lymphocyte is identified under the electron microscope by the presence of cytoplasmic microtubular-like inclusions called parallel tubular arrays (PTA) (Figure 1), and contains Fc-receptors for cytophilic antibody. In this study, lymphocytes containing PTA (PTA-lymphocytes) were quantitated from serial peripheral blood specimens obtained from two patients with Epstein -Barr Virus mononucleosis and two patients with cytomegalovirus mononucleosis. This data was then correlated with the clinical state of the patient.It was determined that both the percentage and absolute number of PTA- lymphocytes was highest during the acute phase of the illness. In follow-up specimens, three of the four patients' absolute lymphocyte count fell to within normal limits before the absolute PTA-lymphocyte count.In one patient who was followed for almost a year, the absolute PTA- lymphocyte count was consistently elevated (Figure 2). The estimation of absolute PTA-lymphocyte counts was determined to be valid after a morphometric analysis of the cellular areas occupied by PTA during the acute and convalescent phases of the disease revealed no statistical differences.

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