CHEMOSYSTEMATIC STUDIES IN THE GENUS PICEA (PINACEAE): II. THE LEAF OIL OF PICEA GLAUCA AND P. MARIANA

1967 ◽  
Vol 45 (9) ◽  
pp. 1703-1714 ◽  
Author(s):  
E. Von Rudloff
Keyword(s):  
Liquid Chromatography ◽  
Distribution Pattern ◽  
Picea Glauca ◽  
Black Spruce ◽  
Gas Liquid Chromatography ◽  
Oil Composition ◽  
Eastern Canada ◽  
Leaf Oil ◽  
Different Populations ◽  
Ecological Differences

The leaf oils from white and black spruce obtained from different locations in Western and Eastern Canada, Michigan, and Minnesota, have been analyzed by gas liquid chromatography. Both species were found to have a remarkably consistent and distinctive distribution pattern of the leaf oil terpenes. The quantitative variations encountered in samples of the same species from different populations are relatively small and ecological differences are not found to affect the leaf oil composition. Hence, analysis of spruce leaf oils appears to be highly suitable for a study of introgression and hybridization.

Chemosystematic studies in the genus Picea (Pinaceae). III. The leaf oil of a Picea glauca × mariana hybrid (Rosendahl spruce)

1968 ◽  
Vol 46 (1) ◽  
pp. 1-4 ◽  
Author(s):  
E. von Rudloff ◽  
M. J. Holst
Keyword(s):  
Liquid Chromatography ◽  
Chemical Composition ◽  
Picea Glauca ◽  
Black Spruce ◽  
White Spruce ◽  
Hybrid Origin ◽  
Gas Liquid Chromatography ◽  
Oil Composition ◽  
Leaf Oil ◽  
Picea Species

The terpene composition of the leaf oils of the Rosendahl spruce, white spruce, and black spruce as isolated from provenances derived from Cromwell, Minnesota, was determined by gas–liquid chromatography. The chemical composition was found to reflect the hybrid origin of the Rosendahl spruce. This confirms that the leaf oil composition of Picea species may serve as a biochemical character in chemo systematic studies.

CHEMOSYSTEMATIC STUDIES IN THE GENUS PICEA (PINACEAE): I. INTRODUCTION

1967 ◽  
Vol 45 (6) ◽  
pp. 891-901 ◽  
Author(s):  
E. von Rudloff
Keyword(s):  
Liquid Chromatography ◽  
Distribution Pattern ◽  
Picea Glauca ◽  
Late Summer ◽  
Gas Liquid Chromatography ◽  
Early Winter ◽  
Optimum Conditions ◽  
Single Tree ◽  
Leaf Oil ◽  
Needle Analysis

The application of volatile leaf oil analyses in chemosystematic studies of North American spruces was investigated. The optimum conditions for sampling and recovery of oil from the foliage of local Picea glauca (Moench) Voss and P. mariana (Mill.) B.S.P. were determined. Each oil was analyzed qualitatively and quantitatively by gas–liquid chromatography. Single needle analysis was also employed. No qualitative and relatively little quantitative variation was found to occur at different sides or heights of a single tree, between trees of different ages, nor at different harvest times between late summer and early winter. Hence, conditions have been found which give a characteristic terpene distribution pattern which may serve as an independent chemotaxonomic character.

Chemosystematic studies in the genus Picea (Pinaceae). IV. The introgression of white and Engelmann spruce as found along the Bow River

1968 ◽  
Vol 46 (7) ◽  
pp. 901-908 ◽  
Author(s):  
R. T. Ogilvie ◽  
E. von Rudloff
Keyword(s):  
Liquid Chromatography ◽  
Distribution Pattern ◽  
White Spruce ◽  
Distribution Patterns ◽  
Quantitative Variation ◽  
Morphological Data ◽  
Gas Liquid Chromatography ◽  
Engelmann Spruce ◽  
Leaf Oil

The leaf oil of the spruces found along the Bow River from the foothills to Bow Summit was analyzed by gas–liquid chromatography. The terpene distribution patterns obtained were compared with the morphological data derived from the cones and twigs of the same trees. The results obtained confirm that typical white spruce occurs at low elevation whereas active hybridization with Engelmann spruce occurs at levels above 5000 ft. Although the trees at the timberline (7200 ft) had predominantly Engelmann spruce characteristics, much variation from tree to tree was encountered. In the terpene composition the quantitative variation was too large to permit conclusions about a typical Engelmann spruce terpene distribution pattern.

Gas-liquid chromatography of terpenes. Part XVI. The volatile oil of the leaves of Juniperus ashei Buchholz

1968 ◽  
Vol 46 (5) ◽  
pp. 679-683 ◽  
Author(s):  
E. Von Rudloff
Keyword(s):  
Liquid Chromatography ◽  
Volatile Oil ◽  
Bornyl Acetate ◽  
Gas Liquid Chromatography ◽  
Juniperus Ashei ◽  
Methylene Groups ◽  
Leaf Oil ◽  
Ashe Juniper ◽  
First Time ◽  
Unique Chemical

The major components of the leaf oil of the Ashe juniper were found to be d-camphor (42.1 %), d-bornyl acetate (22.5%), d-limonene (8.4%), tricyclene (4.8%), d-camphene (4.4%), d-borneol (2.9%), p-cymene (2.8%), d-α-myrcene (1.8%), d-α-pinene (1.7%), and d-camphene hydrate (1.5%). This appears to be the first time that the latter alcohol has been isolated from a natural source. Smaller amounts of linalool, carvone, elemol, and traces of trans-2-methyl-6-methylene-3,7-octadien-2-ol were also identified. Several alcohols having terminal methylene groups were isolated in trace amounts.The monoterpenes found in this oil are not typical for the genus Juniperus and this result offers a unique chemical approach to the study of introgression of the Ashe juniper with other juniper species.

GAS–LIQUID CHROMATOGRAPHY OF TERPENES: PART X. THE VOLATILE OILS OF THE LEAVES OF SITKA AND ENGELMANN SPRUCE

1964 ◽  
Vol 42 (5) ◽  
pp. 1057-1062 ◽  
Author(s):  
E. VON RUDLOFF
Keyword(s):  
Liquid Chromatography ◽  
Sitka Spruce ◽  
Bornyl Acetate ◽  
Gas Liquid Chromatography ◽  
Volatile Oils ◽  
Percentage Composition ◽  
Average Percentage ◽  
Engelmann Spruce ◽  
Leaf Oil ◽  
Aliphatic Esters

The leaf oils from Sitka and Engelmann spruce were obtained in 0.36 and 0.094% yield respectively. The average percentage composition was determined by gas–liquid chromatography. Sitka spruce leaf oil was found to contain mainly d-myrcene (23.5%), l-piperitone (23.2%), and d-camphor (17.2%). Smaller amounts of 1,8-cineole, l-β-phellandrene, p-cymene, two aliphatic esters, d-bornyl acetate, d-borneol, and d-terpinen-4-ol were also isolated and several other esters appeared to be present in small amounts. Engelmann spruce leaf oil was found to contain mainly cis-hex-3-en-1-ol (18.5%), d-camphor (16.0%), d-bornyl acetate (8.6%), d-linalool (5.8%), and two unidentified compounds. Smaller amounts of myrcene, β-phellandrene, p-cymene, piperitone, and several sesquiterpenoids were also recorded. Tricyclene, α-pinene, camphene, β-pinene, 3-carene, limonene, γ-terpinene, and terpinolene were found in small amounts in both oils.Phylogenetic relationships between the different spruces and other conifers are discussed.

Chemosystematic studies in the genus Pinus. IV. Leaf oil composition and geographic variation in jack pine of eastern North America

1982 ◽  
Vol 60 (12) ◽  
pp. 2762-2769 ◽  
Author(s):  
Martin S. Lapp ◽  
E. von Rudloff
Keyword(s):  
North America ◽  
Jack Pine ◽  
Bornyl Acetate ◽  
Eastern North America ◽  
Gas Liquid Chromatography ◽  
Oil Composition ◽  
Leaf Oil ◽  
Euclidean Distances ◽  
The Mean ◽  
Individual Trees

Leaf terpenes of 420 jack pines from 43 sites (Petawawa provenances) in eastern North America were analyzed by gas–liquid chromatography (GLC). In most trees the major components were α-pinene, β-pinene, myrcene, car-3-ene, limonene, β-phellandrene, and bornyl acetate. Eighteen other monoterpenes were identified and 13 sesquiterpenes were characterized by their spectral properties and GLC retention times. These data were analyzed by centroid cluster analysis of the mean-squared Euclidean distances, which showed that jack pine is a diverse species of two major terpene types with eight minor types and a few outlying individual trees. With the exception that the minor clusters and outliers tend to occur at the fringes of the jack pine range, there is no obvious geographic pattern to these clusters.

Recurrence of Spruce Budworm Outbreaks for Two Hundred Years in Western Quebec

1981 ◽  
Vol 57 (6) ◽  
pp. 273-275 ◽  
Author(s):  
J. R. Blais
Keyword(s):  
Twentieth Century ◽  
Picea Glauca ◽  
Radial Growth ◽  
Black Spruce ◽  
Choristoneura Fumiferana ◽  
White Spruce ◽  
Eastern Canada ◽  
Ottawa River ◽  
History Of ◽  
Growth Profiles

The history of spruce bubworm (Choristoneura fumiferana (Clem.)) outbreaks for the past two hundred years in the Ottawa River Valley in Quebec was retraced through radial-growth studies on old white spruce (Picea glauca (Moench)) and black spruce (Picea mariana (Mill.) B.S.P.) trees. The radial-growth profiles clearly indicate three suppression periods resulting from outbreaks that occurred in the twentieth century, each starting about 1910, 1940 and 1967. There is no evidence of an outbreak during the nineteenth century in this region. However, a reduction in radial-growth starting in 1783 observed on the only three specimens of white spruce over 200 years old, has the characteristics of that caused by a budworm outbreak. An interval of 127 years between this and the 1910 outbreak is similar to other long intervals between outbreaks recorded prior to the twentieth century for some other regions in eastern Canada.

sGAS–LIQUID CHROMATOGRAPHY OF TERPENES: PART XI. THE VOLATILE OIL OF THE LEAVES OF JUNIPERUS SCOPULORUM SARG

1964 ◽  
Vol 42 (8) ◽  
pp. 1890-1895 ◽  
Author(s):  
E. von Rudloff ◽  
F. M. Couchman
Keyword(s):  
Liquid Chromatography ◽  
Ornamental Plants ◽  
Rocky Mountain ◽  
Volatile Oil ◽  
Major Constituent ◽  
Gas Liquid Chromatography ◽  
Leaf Oil ◽  
Juniperus Scopulorum

The neutral leaf oil of Rocky Mountain juniper was analyzed by gas–liquid chromatography. d-Sabinene was found to be the major constituent (45.7%) and smaller amounts of d-limonene (11.4%), d-α-pinene (4.2%), γ-terpinene (1.15%), p-cymene (1.4%), l-linalool (1.2%), d-terpinen-4-ol (2.9%), citronellol (0.2%),l-β-elemene (0.2–0.3%), three isomeric cadinenes (2.7%), l-elemol (6.0%), and safrole (1.85%) were isolated. α-Thujene, camphene, car-3-ene, myrcene, α-terpinene, terpinolene, thujone, isothujone, methyl citronellate, sabinyl acetate, sabinol, geraniol, α- and δ-cadinol, and trans-isoeugenol were tentatively identified. An unidentified acetate (II) (4.7%) was isolated from the oxygenated sesquiterpene fraction and another appears to be present in trace amounts.The composition of the oils from the leaves of four local ornamental plants was found to differ significantly from that of the wild juniper.

GAS–LIQUID CHROMATOGRAPHY OF TERPENES: PART XIII. THE VOLATILE OIL OF THE LEAVES OF JUNIPERUS HORIZONTALS MOENCH

1965 ◽  
Vol 43 (5) ◽  
pp. 1017-1021 ◽  
Author(s):  
F. M. Couchman ◽  
E. Von Rudloff
Keyword(s):  
Liquid Chromatography ◽  
Rocky Mountain ◽  
Volatile Oil ◽  
Major Constituent ◽  
Gas Liquid Chromatography ◽  
Leaf Oil

The neutral leaf oil of the creeping juniper from the Prairies was analyzed by gas–liquid chromatography. d-Sabinene (36.5%) was found to be the major constituent, and smaller amounts of d-limonene (17.5%), p-cymene (3.0%), d-α-pinene (1.6%), γ-terpinene (0.4%), d-terpinen-4-ol (4.6%), linalool (0.8%), citronellol (0.8%), γ-cadinene (1.3%), l-elemol (3.8%), α-cadinol or γ-eudesmol (1.6%), and impure α-cyperone (3.9%) were isolated. α-Thujene (3.0%), camphene (0.2%), β-pinene (0.3%), myrcene (3.0%),β-phellandrene (0.2%,), terpinolene (0.1%), 2,4-(8)-p-menthadiene (0.1%), methyl citronellate (0.4%), and geraniol (0.2%) were tentatively identified. The composition of this oil closely resembles that of Rocky Mountain juniper leaves. A small amount of the oil from a hybrid of these two species was analyzed and the possibility of detecting hybridization by analysis of the leaf oil is discussed.

Differentiation of anopheles culicifacies Giles (Diptera: Culicidae) sibling species by analysis of cuticular components

1986 ◽  
Vol 76 (3) ◽  
pp. 529-537 ◽  
Author(s):  
P. J. M. Milligan ◽  
A. Phillips ◽  
D. H. Molyneux ◽  
S. K. Subbarao ◽  
G. B. White
Keyword(s):  
Multivariate Analysis ◽  
Liquid Chromatography ◽  
Discriminant Analysis ◽  
Sibling Species ◽  
Cuticular Hydrocarbon ◽  
Hydrocarbon Composition ◽  
Gas Liquid Chromatography ◽  
Anopheles Culicifacies ◽  
Adult Females ◽  
Ecological Differences

AbstractCuticular wax was extracted from adult females of Anopheles culicifacies Giles and analysed by gas liquid chromatography. The flies were taken from pure cage stocks of three sibling species: 56 individuals of species A, 43 of species B and 51 of species C. The three cytospecies were found to be significantly different in their cuticular hydrocarbon composition by a multivariate analysis of variance. Using discriminant analysis, each cytospecies was characterized by the amounts of C25-C33 hydrocarbons extracted from the cuticle, allowing criteria to be established for the identification of flies. By these criteria, specimens from stocks of known identity were allocated to the correct group with a high success rate. These results may reflect ecological differences among the sibling species.

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