Growth, and patterns of growth and division, in cell suspension cultures of bush bean (Phaseolus vulgaris cv. Contender)

1971 ◽  
Vol 49 (7) ◽  
pp. 1131-1139 ◽  
Author(s):  
Deng-Fong Liau ◽  
W. G. Boll
Keyword(s):  
Cell Suspension ◽  
Growth Regulation ◽  
Single Cells ◽  
Cell Suspension Cultures ◽  
Cell Types ◽  
Growth Cycle ◽  
Cell Number ◽  
Suspension Cultures ◽  
Growth Patterns ◽  
Bush Bean

Changes in cell number, cell size, fresh weight, and dry weight were recorded for the growth cycle of a serially subcultured cell suspension culture derived from hypocotyl of bush bean (var. Contender). The various cell types and growth patterns from single cells were also recorded. The culture showed unique features including a relatively sharp separation of a phase of exponential cell division from a phase of cell expansion. Complete separation of cell clumps gave a free cell culture, of viable cells, by day 16. The growth cycle, cell types, and growth patterns are compared with those in other plant cell suspension cultures. The potential value of the culture for study of growth regulation is discussed.

Extracellular polysaccharide from cell suspension cultures of bush bean (Phaseolus vulgaris cv. Contender)

1972 ◽  
Vol 50 (10) ◽  
pp. 2031-2037 ◽  
Author(s):  
Deng-Fong Liau ◽  
W. G. Boll
Keyword(s):  
Cell Suspension ◽  
Higher Plants ◽  
Cell Suspension Cultures ◽  
Extracellular Polysaccharide ◽  
Cell Number ◽  
Suspension Cultures ◽  
Logarithmic Phase ◽  
Bush Bean ◽  
Culture Cycle ◽  
High Yields

High yields of extracellular polysaccharide were obtained from cell suspension cultures of root, hypocotyl, and cotyledon of bush bean. Hydrolysates of the three polysaccharide samples contained the same sugars: galacturonic acid, galactose, glucose, mannose, arabinose, and xylose. The relative amounts of the six sugars were not the same in the hydrolysates from the three sources. The extracellular polysaccharide was produced at all times during the culture cycle. Semilogarithmic plots of increase in cell number, and production of extracellular polysaccharide, indicate that production per cell decreased during the logarithmic phase, and increased at the onset of the stationary phase. Production of extracellular polysaccharide, per culture and per cell, was much higher than that reported for other cell cultures of higher plants.

Callus and cell suspension culture of bush bean (Phaseolus vulgaris)

1970 ◽  
Vol 48 (6) ◽  
pp. 1119-1130 ◽  
Author(s):  
Deng-Fong Liau ◽  
W. G. Boll
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Dry Weight ◽  
Cell Number ◽  
Callus Cultures ◽  
Suspension Cultures ◽  
Coconut Milk ◽  
Mineral Salts ◽  
Bush Bean

A solid medium was developed for callus cultures originating from explants of root, hypocotyl, and cotyledon of seedlings of bush bean, and a liquid medium was developed for the growth of cell suspension cultures derived from the callus cultures. Some unsatisfactory media are recorded. Concentrations of mineral salts for cell suspension cultures are lower than for callus cultures. Both coconut milk and other organic substances are required for maximum growth. With cell suspensions the effect of deproteinized coconut milk is the same as that of raw coconut milk but, with callus cultures, deproteinized coconut milk gives greater yield. There are no obvious differences in yield of callus derived from root, hypocotyl, or cotyledon. Few differences in yield were obtained between cell suspension cultures from root, hypocotyl, and cotyledon but those from root gave the highest yield in dry weight. However, in the same medium, cells from the three origins are very similar in form and appearance. Some effects of different media on cell form and clumping are described. The yield in suspension culture is very high. Increase in cell number, fresh weight, and dry weight is about 100-fold in 12 days involving about six to seven divisions per cell.

scholarly journals Effects of Plant Growth Regulators and Sugars on Ehretia asperula Zoll. et Mor. Cell Cultures

2021 ◽  
Author(s):  
P.T.M. Tram ◽  
N.K. Suong ◽  
L.T.T. Tien
Keyword(s):  
Cell Suspension ◽  
Callus Induction ◽  
Malignant Tumors ◽  
Single Cells ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Dichlorophenoxyacetic Acid ◽  
Human Immune System ◽  
Friable Callus ◽  
2,4 Dichlorophenoxyacetic Acid

Background: Belonging to the Boraginacae family, Ehretia asperula Zoll. et Mor., called “Xa den”, is a precious medicinal plant also known as the “cancer tree” by the Muong ethnic group in Hoa Binh Province, Vietnam. Xa den has been demonstrated to inhibit the development of malignant tumors, reduce oxidation and enhance the human immune system. This research focused on examining friable callus induction from young stems of Ehretia asperula Zoll. et Mor. Methods: Samples of Xa den were less than two years old. Young stems with 2 to 6 leaves served as explants for callus induction. Explants placed on autoclaved B5 nutrients incubated at 25oC, in the dark. The testing factors were concentrations of 2,4-Dichlorophenoxyacetic acid (2,4-D) and Benzyl adenine (BA), types and concentrations of sugars.Result: Friable callus was induced on B5 medium with 0.4 mg/L of 2,4-D, 0.1 mg/L of BA and 30 g/L of glucose at the highest rate (100%). Additionally, callus grew best after 5 weeks of culture weighing 0.194 g. Friable callus was used as material for cell suspension cultures. After two weeks, the Xa den cell suspension cultures contained single cells and small cell clumps. The liquid medium had changed from dark yellow to light brown.

Effects of Plant Bioregulators on the Production of Iridoid Derived Terpenoids in Valeriana wallichii and Fedia cornucopiae Cell Suspension Cultures

1987 ◽  
Vol 42 (1-2) ◽  
pp. 33-40 ◽  
Author(s):  
Wolfram Förster ◽  
Hans Becker
Keyword(s):  
Cell Suspension ◽  
Cell Cultures ◽  
Exponential Growth ◽  
Cell Suspension Cultures ◽  
Growth Cycle ◽  
Suspension Cultures ◽  
Tissue Cultures ◽  
Cell Systems ◽  
Optimal Activity ◽  
Plant Tissue Cultures

Abstract Four plant bioregulators were tested for their effects on production of valepotriates in Valeriana wallichii and Fedia cornucopiae cell suspension cultures. Concentrations of more than 10 ppm reduced valepotriate yield. At lower concentrations production was increased. For optimal activity, bioregulators had to be applied during early exponential growth, up to day 8 of the growth cycle. At equimolar concentrations dim ethylm orpholinium bromide (4 ppm) and dimethylpiperidinium chloride (3 ppm) significantly im proved total valepotriates in V. wallichii (up to 23%) and in F cornucupiae (up to 50% ) 2-(3,4-dichlorophenoxy ) - triethylamine (6 ppm ) and 2-(3,5-diisopropylphenoxy)-triethylam ine (6.4 ppm) increased valepotriate production in both cell cultures up to 40%. With dimethylpiperidinium chloride and dimethylmorpholinium bromide the ratio of m onoene to diene valepotriates in both cell systems was significantly shifted to the m onoene com pounds. A general use of these bioregulators to increase production of terpenoid secondary m etabolites in plant tissue cultures is indicated.

Sterol and sesquiterpenoid biosynthesis during a growth cycle of tobacco cell suspension cultures

1989 ◽  
Vol 8 (1) ◽  
pp. 48-52 ◽  
Author(s):  
Joseph Chappell ◽  
Carol Von Lanken ◽  
Urs Vögeli ◽  
Prashant Bhatt
Keyword(s):  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Growth Cycle ◽  
Suspension Cultures ◽  
Tobacco Cell ◽  
Tobacco Cell Suspension

scholarly journals Cell suspension culture of Amsonia tabernaemontana Walter: growth, organogenesis and alkaloid production

2014 ◽  
Vol 50 (4) ◽  
pp. 615-624 ◽  
Author(s):  
Mirosława Furmanowa ◽  
Lucyna Rapczewska
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Cell Suspension Cultures ◽  
Cell Number ◽  
Growth Increment ◽  
Suspension Cultures ◽  
Cell Suspensions ◽  
Alkaloid Production ◽  
Cellular Aggregates

The paper discusses the growth of cell suspension cultures of <em>Amsonia tabernaemontana</em> Walter established from callus of hypocotyl origin. The cell number and growth increment were determined. Cellular aggregates developed well in the Wood and Braun (WB) medium with 1 mg/l NAA and 0.5 mg/l kinetin (growth increment 712.4). When the aggregates were cultured on WB media without NAA and kinetin or with 0.02 mg/l kinetin and 3 mg/l IAA, Toots developed an the aggregates. Examiination of the roots and cell suspensions indicates that the Toots are richer in alkaloids than the callus and cell suspensions.

Changes in functional properties of mitochondria during growth cycle of Arabidopsis thaliana cell suspension cultures

1998 ◽  
Vol 36 (5) ◽  
pp. 347-356 ◽  
Author(s):  
Jacques Davy de Virville ◽  
Marie-Françoise Alin ◽  
Yvonne Aaron ◽  
René Rémy ◽  
Thérése Guillot-Salomon ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Suspension ◽  
Functional Properties ◽  
Cell Suspension Cultures ◽  
Growth Cycle ◽  
Suspension Cultures

Effect of either 2,4-dichlorophenoxyacetic acid or kinetin on production and composition of various fractions from extracellular polysaccharides produced by cotyledon cell suspension cultures of bush bean (Phaseolus vulgaris cv. Contender)

1978 ◽  
Vol 56 (15) ◽  
pp. 1816-1822 ◽  
Author(s):  
S. Mante ◽  
W. G. Boll
Keyword(s):  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Extracellular Polysaccharide ◽  
Suspension Cultures ◽  
Extracellular Polysaccharides ◽  
Dichlorophenoxyacetic Acid ◽  
Neutral Sugar ◽  
Bush Bean ◽  
Cotyledon Cell ◽  
2,4 Dichlorophenoxyacetic Acid

Cotyledon cell suspension cultures of bush bean required 2, 4-dichlorophenoxyacetic acid (2,4-D) for growth. Kinetin was not essential but was required for optimum growth. Both of the regulators were required for optimum production of extracellular polysaccharide (EP).The two regulators had different effects upon the production of three polysaccharide fractions (two pectins and a neutral polysaccharide) isolated from the EP at various stages of the culture cycle.The neutral sugar composition of the pectin fractions from all treatments, including regulator treatments, showed considerable fluctuation during the culture cycle and could be changed markedly by regulator treatments. Changes in composition of the neutral polysaccharide were slight. As a consequence of these results it is now possible to obtain cells differing in the nature of the systems synthesizing, or controlling the synthesis of, the polysaccharides.

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