Distribution of cellular amino acids, protein, and total organic nitrogen during fruitbody development in Flammulina velutipes. I. Growth on sawdust medium

1982 ◽  
Vol 60 (8) ◽  
pp. 1330-1341 ◽  
Author(s):  
Hans E. Gruen ◽  
William M. Wong
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Organic Nitrogen ◽  
Amino Nitrogen ◽  
Dry Weight ◽  
Flammulina Velutipes ◽  
Total Organic Nitrogen ◽  
Protein Amino Acid ◽  
Protein Amino Acids

Dry weights of stipes and pilei of Flammulina velutipes grown on nutrient-supplemented sawdust increased throughout the growth period. The pilei weighed only slightly less than the stipes. Amounts of total organic nitrogen, α-amino nitrogen, and alkali-soluble protein increased in the whole pileus and stipe as the fruitbodies elongated, but the concentrations on a dry weight basis decreased although they were always highest in the pileus. The concentration of alkali-insoluble nitrogen increased in both structures and was highest in the stipe. Concentrations of total nitrogen and protein in surface mycelium did not change significantly when fruitbodies formed but increased markedly when their growth ceased. An average of 8.3 mg dry weight of spores containing about 0.6 mg of nitrogen was released during the life of the fruitbody. Changes in 18 free and 17 protein amino acids were followed quantitatively in the pileus and stipe. Glutamic and aspartic acids and alanine were always among the four predominant free amino acids. Lysine and arginine concentrations remained low in the stipes but increased considerably in the expanding pilei. Ornithine levels increased strongly in the stipe during the early part of rapid elongation but remained almost constant and low in the pilei. Free proline was detected only in traces. Valine became the most abundant protein amino acid during elongation, especially in the stipe. There was very little bound methionine and cystine. In surface mycelium levels of free amino acids were low before fruiting and close to the end of fruitbody growth. Protein amino acids increased during that interval but their proportions remained virtually unchanged and valine was not predominant. The concentration of urea remained very low in both pilei and stipes during their growth.

Distribution of cellular amino acids, protein, and total organic nitrogen during fruit body development in Flammulina velutipes. II. Growth on potato–glucose solution

1982 ◽  
Vol 60 (8) ◽  
pp. 1342-1351 ◽  
Author(s):  
Hans E. Gruen ◽  
William M. Wong
Keyword(s):  
Amino Acids ◽  
Organic Nitrogen ◽  
Glucose Solution ◽  
Fruit Body ◽  
Amino Nitrogen ◽  
Dry Weight ◽  
Early Growth ◽  
Flammulina Velutipes ◽  
Total Organic Nitrogen ◽  
Rapid Elongation

In Flammulina velutipes grown on potato–glucose solution the dry weight of mycelium decreased when fruitbodies elongated rapidly. Loss of dry weight by aborted primordia and stunted fruitbodies paralleled gains by large fruitbodies. These gains exceeded the losses by the rest of the colony and indicate that large fruitbodies derived their substrates both from the medium and from materials stored in the rest of the colony. Close to 43% of the organic nitrogen in the medium was consumed before fruitbodies formed and only 8% remained at the end of their growth. Concentrations of total nitrogen, α-amino nitrogen, and alkali-soluble protein declined in the mycelium during most of the growth of the fruitbodies and then remained constant except for a slight final increase in protein. Concentrations of these fractions also decreased in the small fruitbodies and stipes of large fruitbodies but increased in the pilei. Changes in total amounts of these fractions show that gains by all fruitbodies during elongation exceeded the losses by the mycelium and that part of their nitrogen continues to be derived from the medium. Changes in 18 free and 17 protein amino acids were followed quantitatively in the colony. Free arginine, glutamic acid, ornithine, and alanine predominated in the mycelium and small fruitbodies during early growth of the latter, but arginine and ornithine decreased steeply thereafter. During rapid elongation of the large fruitbodies the concentration of arginine increased strongly in the pilei. The increase was much less in the stipes, but successive changes in the rest of the colony suggest that arginine is translocated into the large pilei from the mycelium and possibly the small fruitbodies. Aspartic and glutamic acids, leucine, and alanine predominated in protein of the whole colony until the early growth of fruitbodies, but protein composition changed during rapid elongation. The proportion of valine increased in the mycelium and it became the most abundant bound amino acid in large stipes. [14C]Leucine was fed in replacement medium and incorporation of the label was determined in nitrogenous fractions of different colony portions at the onset and termination of fruitbody growth. Most of the label transported into fruitbodies was in the ethanol-soluble free amino acids.

CHANGES IN PEACH TREES (cv. RED HAVEN) ATTACKED BY TAPHRINA DEFORMANS, WITH PARTICULAR REFERENCE TO NITROGEN METABOLISM IN INFECTED AND NON-INFECTED LEAVES

1967 ◽  
Vol 45 (4) ◽  
pp. 459-477 ◽  
Author(s):  
Vittorio Raggi
Keyword(s):  
Amino Acids ◽  
Nitrogen Metabolism ◽  
Free Amino Acids ◽  
Free Amino ◽  
Dry Weight ◽  
Infected Tissue ◽  
Diseased Plant ◽  
Glycolytic Activity ◽  
Protein Amino Acids ◽  
Peach Trees

Some of the changes induced by Taphrina deformans on metabolism (particularly the nitrogen metabolism) of peach leaves were studied. Observations were as follows. (1) Dry weight diminishes in the infected tissues, especially at the end of the cycle, whereas it remains almost unchanged in the non-infected tissues of the diseased plant. (2) Initially, the amount of total nitrogen, per gram of dry substance is clearly greater in the infected tissues than in the control, then it becomes lower during the differentiation of the asci, especially at the stage of full sporulation. Similar variations occur also, in a more attenuated form, in the non-infected tissues. (3) A considerable increase of the glycolytic activity is observed in the infected tissue especially during the period preceding the differentiation of the asci, and an increase of respiration, even though much more restricted, is observed also in the next period. In non-invaded tissues, an increase of glycolysis is noted along with a diminution of respiration. (4) The non-infected leaves weigh more than those of the control until the differentiation of the asci; after it, they have a clear drop up to the maturity of the asci, and a further revival at the end of the disease. (5) In the infected tissues, the content of free amino acids is always higher than in the control (with a maximum after sporulation), whereas in the non-infected ones it is generally lower. (6) The more important alterations in the pool of the free amino acids in invaded tissues consist in a scarcity, in percentage of the total, of alanine and glutamic acid, especially at the end of the disease, whereas proline, ornithine, glycine, and others increase, especially during the incubation period. In particular the glutamine–asparagines– threonine group reaches the highest value when sporulation has occurred. In the non-invaded tissues, the changes are more restricted. (7) The content of protein amino acids in infected tissues is generally lower than in the control, especially in the last phases of infection whereas in the non-invaded parts it is generally higher, except in differentiation of the asci. (8) In the infected tissue, the alterations of the quantitative relationships between the single protein amino acids (in percentage of the total) are not very great, but some diminutions and increases are recorded (diminution of alanine, glycine, leucine, etc.; increase of lysine, histidine, etc.); in the non-infected tissues, no noteworthy alterations were observed.

AMINO ACID ANALYSES OF THE SPECIES IN THE GENUS GOSSYPIUM

1968 ◽  
Vol 10 (2) ◽  
pp. 362-368 ◽  
Author(s):  
Patricia Sarvella ◽  
B. J. Stojanovic
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Butyric Acid ◽  
Free Amino Acids ◽  
Free Amino ◽  
Protein Amino Acid ◽  
Amino Butyric Acid ◽  
Dna Base ◽  
Protein Amino Acids

The free and protein amino acid patterns present in the leaves of the species of the genus Gossy pium were determined. The presence or absence of the free amino acids except for glycine and γ-amino butyric acid did not appear to be of value for separation of the species. However, the patterns of the protein amino acids varied between the genomes, and seemed to separate the species. These patterns were separated into groups similar to those found for the DNA-base ratios and the cytotaxonomic grouping of the species.

scholarly journals Composition and content of free amino acids of sublittoral red algae from Murmansk coast of the Barents Sea.

2020 ◽  
Vol 11 (5-2020) ◽  
pp. 92-102
Author(s):  
M.P. Klindukh ◽  
◽  
I.V. Ryzhik ◽  
E.O. Dobychina ◽  
◽  
...  
Keyword(s):  
Amino Acids ◽  
Red Algae ◽  
Free Amino Acids ◽  
Free Amino ◽  
Barents Sea ◽  
Qualitative Composition ◽  
The Barents Sea ◽  
Protein Amino Acids

Data on the content of free amino acids (FAA) in four species of red algae are presented. The content of 20 amino acids was determined in the composition of FAA of the studied species. The largest amounts contained glutamic and aspartic acids, alanine, proline and taurine. The studied algae do not differ in the qualitative composition of protein amino acids, but differ in the presence and content of nonprotein ones. The content of most of the FAA and their summ was the highest for Phycodrysrubens, and in Palmaria palmatathe smallest.

EXTRACELLULAR ORGANIC NITROGEN IN USTILAGO MAYDIS FERMENTATION BROTHS

1956 ◽  
Vol 34 (1) ◽  
pp. 1195-1198
Author(s):  
Eugene L. Dulaney ◽  
E. Bilinski ◽  
W. B. McConnell
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Organic Nitrogen ◽  
Ustilago Maydis ◽  
Ammonia Nitrogen ◽  
Small Peptides ◽  
Lysine Concentration ◽  
High Level ◽  
Fermentation Broths

Free amino acids and small peptides make up most of the extracellular organic nitrogen in media from shaken and aerated Ustilago maydis fermentations. Of the 3.5 mgm./ml. ammonia nitrogen added, 2.9 mgm./ml. remained in the extracellular broth. This extracellular nitrogen contained 1.17 mgm./ml. of organic nitrogen and 1.74 mgm./ml. of residual ammonia nitrogen. At least 53% of extracellular organic nitrogen is in the form of free amino acids. Fifteen amino acids were estimated quantitatively in acid-hydrolyzed broth and a particularly high level of arginine (1.14 mgm./ml.) was found. The amounts of methionine and tryptophan in the broth were quite low but the lysine concentration 0.400 mgm./ml. was relatively high.

The effect of starvation and low nitrogen intakes on the concentration of free amino acids in the blood plasma and on the nitrogen metabolism in sheep

1970 ◽  
Vol 21 (5) ◽  
pp. 723 ◽  
Author(s):  
J Leibholz
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Dry Matter ◽  
Control Diet ◽  
Amino Nitrogen ◽  
Essential Amino Acids ◽  
Isoleucine Leucine ◽  
Plasma Urea ◽  
Low Nitrogen

Crossbred wethers were given a control diet (8 g nitrogen, 730 g dry matter daily) or a low nitrogen diet (0.5 g nitrogen, 520 g dry matter daily) or starved, for a 12 or 20 day experimental period. The concentrations of free serine, glutamine, glycine, alanine, histidine, and arginine in the plasma of the starved sheep decreased significantly while the concentrations of lysine, 3-methylhistidine, and isoleucine increased significantly. The ratio of essential to non-essential amino acids increased from 0.35 to 0.56 in the starved sheep. In sheep on the low nitrogen diet, the ratio of essential to non-essential amino acids in the plasma decreased from 0.40 to 0.27, with significant increases in the concentrations of glutanlic acid, glutamine, glycine, isoleucine, leucine, and 3-methylhistidine. Starvation and the low nitrogen diet both resulted in a reduction of the plasma urea concentrations. Starvation and the low nitrogen diet resulted in a 20-50 % reduction in the flow of saliva and a 40-78% increase in the concentration of total nitrogen. This resulted in no significant change in the daily secretion of nitrogen in the saliva. The concentration of urea in the saliva was increased by 3-54%. The concentrations of individual free amino acids in saliva are reported. The nitrogen content of the rumen was reduced, and after 7 days of starvation or on the low nitrogen diet all rumen nitrogen could be attributed to ammonia and free �-amino nitrogen.

The causes of buoyancy in eggs of marine teleosts

1987 ◽  
Vol 67 (1) ◽  
pp. 169-182 ◽  
Author(s):  
J. C. A. Craik ◽  
S. M. Harvey
Keyword(s):  
Amino Acids ◽  
Water Content ◽  
Free Amino Acids ◽  
Free Amino ◽  
Sea Water ◽  
Inorganic Ions ◽  
Dry Weight ◽  
High Water Content ◽  
Demersal Eggs ◽  
Pelagic Eggs

Pelagic eggs and demersal eggs of teleosts both have osmotic concentrations similar to that of the maternal body fluids, less than half that of sea water. Pelagic eggs are buoyant because they contain such large quantities of this dilute aqueous fluid. While the demersal eggs of teleosts usually have a water content of 60–70%, the buoyant pelagic eggs of marine teleosts such as whiting, Norway pout, saithe, cod, haddock, turbot, dab, plaice, witch, long rough dab, halibut and sole typically have a very high water content (ca. 92 %) and a lipid content of 10–17% of egg dry weight. About 90% of the buoyancy of such eggs in sea water is caused by their high aqueous content, only about 10% being caused by lipid. The buoyant eggs of grenadier and ling have large oil globules and higher lipid contents, 27 and 35 % of dry weight respectively. Nevertheless, most of their buoyancy is provided by their high aqueous contents (89 and 81 % water). The high water content of pelagic eggs is brought about by a massive influx of water into the oocytes during meiotic maturation (ripening) after vitellogenesis but before ovulation. In cod and plaice, ripening is accompanied by a four- to five-fold increase in both water content and free amino-acids, and by a large influx of both potassium and sodium. In cod, free amino-acids contribute much more than these inorganic ions to the water influx and to the total osmotic concentration of the mature egg, but in plaice the relative contribution of inorganic ions approaches that of the free amino-acids.

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