Effects of osmolality, cytokinin, and organic acids on pollen callus formation in Triticale anthers

1983 ◽  
Vol 61 (3) ◽  
pp. 639-641 ◽  
Author(s):  
Y. C. Chien ◽  
K. N. Kao
Keyword(s):  
Tissue Culture ◽  
Organic Acids ◽  
Callus Induction ◽  
Treatment Effects ◽  
Callus Formation ◽  
Optimal Conditions ◽  
Benzyl Adenine ◽  
Growing Conditions

Triticale anthers with pollen at middle to late uninucleated stages were cultured individually in Falcon micro test II tissue culture plates. The results indicate that when the anthers were cultured in the same growing conditions the differences in pollen callus formation among anthers from the first and second florets in the same spikelet were not statistically significant, whereas the differences in callus formation among anthers from different spikes (or plants) were statistically significant. These results suggest that comparisons of treatment effects should be made between samples consisting of anthers from the same spikelet only. Benzyl adenine (BA) and higher osmolality enhanced pollen callus induction, while a higher concentration of organic acids suppressed it. Under optimal conditions 31% of anthers formed callus, while the number of pollen calli per 100 seeded anthers was as high as 130. The pollen calli were able to develop into plants; however, the frequency was relatively low.

scholarly journals Sterilization and Callus Formation of Rubber Meristem (Hevea brasiliensis Muell. Arg.)

2018 ◽  
Vol 6 (4) ◽  
pp. 1
Author(s):  
Dwi Sucianingtyas Sukamto ◽  
Lila Maharani ◽  
Siti Amalia ◽  
Sholeh Avivi ◽  
Didik Pudji Restanto
Keyword(s):  
Tissue Culture ◽  
Callus Induction ◽  
Hevea Brasiliensis ◽  
Callus Formation ◽  
Rubber Plantation ◽  
Sterile Water ◽  
Mass Propagation ◽  
Culture Techniques ◽  
Embryonic Callus ◽  
Tissue Culture Techniques

Rubber plant (Hevea brasiliensis Muell Arg) is one of the important plantation commodities in Indonesia because of its role as a source of income. It stimulates the economic growth around the rubber plantation area. The propagation of rubber is still using conventional methods like grafting. The technique of tissue culture through callus induction is one of the alternatives of mass propagation of rubber seedling with quick and efficient time. The sterilization method is very important to determine the success of tissue culture techniques. Therefore, the aim of this research is to know the best method of sterilization and callus formation in rubber explants. The basic media used were WPM and MS, with BAP of 2 ppm and NAA 0.1 ppm. The best result of sterilization is by soaking 5% fungicide solution for 5 minutes, 5% Clorox solution for 15 minutes, betadin 10% solution for 5 minutes, and finally it rinsed with sterile water three times. The best medium uses WPM medium for callus induction, with 0.5 cm callus length and embryonic callus. In contrast, the MS medium has 0.4 cm callus length and non embryonic callus.

scholarly journals Regenerasi dan Pertumbuhan Beberapa Varietas Tebu (Saccharum officinarum L.) secara In Vitro

2011 ◽  
Vol 7 (2) ◽  
pp. 106 ◽  
Author(s):  
Deden Sukmadjaja ◽  
Ade Mulyana
Keyword(s):  
Tissue Culture ◽  
In Vitro Culture ◽  
Callus Induction ◽  
Callus Formation ◽  
Saccharum Officinarum ◽  
Sugarcane Varieties ◽  
Media Formulation ◽  
Research Activities

<p>(Saccharum officinarum L.) through In Vitro Culture.<br />Deden Sukmadjaja and Ade Mulyana. The research was<br />conducted at the Laboratory of Tissue Culture The Biology of<br />Cell and Tissue Researcher Group ICABIOGRAD, Bogor from<br />June to November 2009 to studied growth and regenerations<br />response some varieties of sugarcane through in vitro<br />culture. The research activities have been carried out in<br />three steps, i.e., callus formation, regeneration of shoots and<br />roots regeneration. The type of explants used in the study<br />was in vitro planlet explants of both sugarcane varieties.<br />Seven media formulations were used for the callus induction<br />and regeneration of shoots, while five media formulations<br />were used for the roots regeneration. The results<br />showed that the highest respond for calluses induction was<br />Bulu Lawang varieties at media formulation MS + 2.4-D 2<br />mg.l-1 + BAP 0.4 mg.l-1 + CH 2000 mg.l-1 and PS 951 varieties<br />at media formulation MS + 2.4-D 1 mg.l-1 + BAP 0.4 mg.l-1.<br />While the highest respond for regeneration of shoots was<br />Bulu Lawang varieties at media formulation MS0 (control<br />MS) dan PS 951 varieties at media formulation MS + BAP 1<br />mg.l-1 + kinetin 1 mg.l-1 + NAA 0.5 mg.l-1 + GA3 0.5 mg.l-1.<br />The highest respond of roots regeneration was Bulu Lawang<br />and PS 951 varieties at media formulation MS + IBA 1 mg.l-1.<br />Acclimatization of plantlets produced were grew successfully<br />about 90-100% in greenhouse.</p>

scholarly journals Effect of Benzyl Adenine Concentration on Callus Induction of Geranium Plants (Pelargonium graveolens L'Her) from Petiole and Leaf Explants

2020 ◽  
Vol 10 (1) ◽  
pp. 20-23
Author(s):  
Moch Faizul Huda ◽  
◽  
Serafinah Indriyani
Keyword(s):  
Callus Induction ◽  
Culture Medium ◽  
Callus Formation ◽  
Leaf Explants ◽  
Ms Medium ◽  
Morphological Parameters ◽  
Benzyl Adenine ◽  
Petiole Explants ◽  
Pelargonium Graveolens ◽  
Light Green

Geranium plant (Pelargonium graveolens L'Her) is one of the geranium oil producing plants that has many benefits. Callus culture is a technique that can be used to plant multiplication and increase production of secondary metabolites. This study aims to determine the effect of the concentration of Benzyl Adenine on the formation of geranium callus from petiole and leaf explants. Callus induction was carried out by culturing petiole and leaf explants on MS medium + 0.1 mg.L-1 NAA + Benzyl Adenine (0; 0.5; 1; 1.5 and 2 mg.L-1). Callus morphological parameters, percentage of callus formation, and time of first callus formation were observed. The formation of geranium callus influenced by the explant type and the concentration of Benzyl Adenine. In the 2nd week, the geranium callus was initiated, light green colored with a compact callus texture. At 4th week, the percentage of callus formation containing NAA 0.1 mg.L-1 of petiole and leaf explants was 20% and 8%, whereas the percentage of callus formation on medium containing 0.1 mg.L-1 NAA combined with 0.5-2 mg.L-1 Benzyl Adenine of petiole and leaf explants was 52-80% and 24-52%. The best percentage of callus formation was found on the culture medium containing 1 mg.L-1 Benzyl Adenine, equaled 80% of petiole explants, and 52% of leaf explants.

scholarly journals Induksi Kalus dan Optimasi Regenerasi Empat Varietas Padi melalui Kultur In Vitro

2016 ◽  
Vol 2 (2) ◽  
pp. 74 ◽  
Author(s):  
Ragapadmi Purnamaningsih
Keyword(s):  
Tissue Culture ◽  
Callus Induction ◽  
Root Formation ◽  
Callus Formation ◽  
Shoot Multiplication ◽  
Research Activities ◽  
Callus Regeneration ◽  
Plant Acclimatization ◽  
Medium Formulation

<p class="p1">A study was conducted at the Tissue Culture Laboratory of ICABIOGRAD, Bogor, to obtain an optimum medium formulation for calli regenerations of for rice varities (Ciherang, Cisadane, IR64, and T-309). The research activities were done in five steps, i.e., callus induction, callus regeneration, shoot multiplication, root formation, and plant acclimatization. The type of explants used in the study was embriozygotic explants. Five media formulations were used for the callus induction, while four media formulations were used for the callus regeneration. The results showed that the best medium formulation for induction of callus formation was MS + 2,4-D 2 mg/l + casein hidrolisat 3 mg/l, while the best medium formulation for callus regeneration was MS + BA 3 mg/l + thidiazuron 0,1 mg/l.</p>

scholarly journals The regeneration of Acer rubrum L. "October Glory" through embryonic callus

2020 ◽  
Author(s):  
Chongwen Dai ◽  
Yang-yang YAN ◽  
Yumin Liu ◽  
Ya-min LIU ◽  
Yuan-wei DENG ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Callus Induction ◽  
Callus Formation ◽  
Acer Rubrum ◽  
Adventitious Bud ◽  
Naphthaleneacetic Acid ◽  
Stem Segment ◽  
Embryonic Callus ◽  
Bud Induction ◽  
Culture Process

Abstract Background: Tissue culture and rapid propagation technology is an important way to solve the difficulties of plant propagation. This experiment aims to explore the appropriate conditions at each stage of the red maple’s tissue culture process and to obtain plantlets, thus providing a theoretical basis for the establishment of the red maple’s tissue culture system. Results: The results showed that the stem segment is the most suitable explant for inducing embryogenic callus. The MS (Murashige&Skoog) +0.8 mg/L TDZ (Thidiazuron) +1.0 mg/L 6-BA (6-Benzylaminopurine) +0.5 mg/L IAA(Indole-3-acetic acid) +35 g/L sucrose+7.5 g/L semi-fixed medium was the best for callus formation. When selecting type Ⅵ callus as embryonic callus induction material, MS+0.6 mg/L TDZ+0.5 mg/L 6-BA +2.0 mg/L IAA +35 g/L sucrose+7.5 g/L semi-fixed medium can get embryonic callus. The optimal medium for adventitious bud induction is MS+1.0 mg/L TDZ+3.0 mg/L 6-BA+0.2 mg/L NAA (1-Naphthaleneacetic acid)+1.2 mg/L IAA+35 g/L sucrose+7.5 g/L semi-fixed medium. The induction rate of adventitious roots in MS+0.6 mg/L TDZ+1.0 mg/L 6-BA+3 mg/L NAA+35 g/L sucrose+7.5 g/L semi-fixed medium was the highest, reaching 76%. Conclusions: In the course of our research, we found that PGRs play an important role in the callus induction stage, and the effect of TDZ is particularly obvious; The callus cells grow and proliferate according to the "S" growth curve, and can be sub-cultured when the highest growth point is reached to maintain the rapid proliferation of the callus cells and to avoid inactivation of callus caused by tight niche.

scholarly journals Callus formation and organogenesis in tissue culture Deschampsia antarctica E. Desv.

2019 ◽  
Vol 17 (1) ◽  
pp. 8-15
Author(s):  
I. I. Konvalyuk ◽  
L. P. Mozhylevs’ka ◽  
V. A. Kunakh
Keyword(s):  
Tissue Culture ◽  
Callus Induction ◽  
Shoot Growth ◽  
Callus Formation ◽  
Leaf Explants ◽  
Deschampsia Antarctica ◽  
Donor Plant ◽  
Growth Point ◽  
The Media

Aim. The aim of the work was to determine the optimal conditions for induction and proliferation of tissue culture obtained from D. antarctica plants from various localities of the Maritime Antarctica. Methods. Tissue and organ culture techniques. Results. The media В5 supplemented with 2 mg/l 2,4-D + 0,1 mg/l BAP, В5 supplemented with 10 mg/l 2,4-D + 0,2 mg/l BAP and МС, supplemented with 5 mg/l 2,4-D + 0,1 mg/l Kin were optimal for callus induction from different types of explants. The media with a reduced concentrations of auxins and cytokinins were the most effective for maintenance of continuous tissue culture compared to the media for callus induction: B5 + 2 mg/l 2,4-D mg/l + 0,1 mg/l BAP and MC + 1 mg/l 2,4-D + 0.1 mg/l Kin. Tissues from shoot growth point and leaf explants of genotypes DAR12a and G/D12-2a on medium B5 with 2 mg/l 2,4-D + 0.1 mg/l BAP and B5 with 10 mg/l 2,4-D + 0,2 mg/l BAP demonstrated the ability to spontaneous organogenesis and formed separate shoots. Conclusions. Conditions have been determined for the induction and proliferation of tissue culture from leaf, root, and shoot growth point explants of D. antarctica. The frequency of callus formation depended on the mineral composition of medium, ratios and concentrations of growth regulators, type of explant, and genotype of a donor-plant. As a result of spontaneous organogenesis, regenerated plants were obtained, conditions for their rooting in vitro were elaborated. The proposed methods for induction and proliferation tissue culture of D. antarctica in vitro can be used to produce the plant material useful for a various investigations. Keywords: Deschampsia antarctica E. Desv., tissue culture, organogenesis in vitro, frequency of callogenesis.

scholarly journals Induction and growth pattern of callus from Piper permucronatum leaves

2016 ◽  
Vol 18 (1) ◽  
pp. 142-148 ◽  
Author(s):  
M.R.A. SANTOS ◽  
M.C.M. GUIMARÃES ◽  
E.S. PAZ ◽  
G.M.O. MAGALHÃES ◽  
C.A. SOUZA ◽  
...  
Keyword(s):  
Growth Pattern ◽  
Callus Induction ◽  
Callus Formation ◽  
Leaf Explants ◽  
Cell Suspension Cultures ◽  
Callus Growth ◽  
Deceleration Phase ◽  
Stomach Pain ◽  
Perennial Shrub ◽  
A Cell

ABSTRACT Piper permucronatum is a perennial shrub, a medicinal plant native to the Amazon Rainforest. Traditionally, the tea of its leaves is used to combat menstrual and intestinal cramps, stomach pain, digestive problems, diarrhea, hemorrhage, and nausea. Its leaf’s essential oil is effective against Aedes aegypti larvae; its flavones and flavanones have a fungicidal effect against Clamidosporium cladosporioides and C. sphaerospermum; its hexanic extract is effective against Leishmania amazonensis. The objective of this study was to provide a protocol for callus induction from P. permucronatum leaves and an identification of the callus growth pattern, focusing on the deceleration phase, when the callus cells must be subcultured into liquid medium in order to produce a cell suspension cultures. Leaf explants were inoculated in a solid MS medium supplemented with factorial combinations of 2,4-D, BA, NAA and GA3. Callus formation was evaluated weekly until the 49th day. Subsequently, new explants were inoculated at the hormonal combination that resulted in the highest callus cell proliferation and, every seven days during a period of 70 days, samples were dried and weighed to determine the callus growth pattern. NAA and GA3 were not effective for callus induction. Combinations of 2,4-D and BA resulted in callus induction and proliferation. The highest percentage of callus induction was observed with the combination of 4.52 µM 2,4-D and 4.44 µM BA. The calluses thereby produced were friable and whitish. The callus growth pattern followed a sigmoid shape. The deceleration phase started on the 56th day of culture.

scholarly journals PROPAGATION STRATEGIES OF TRIBULUS TERRESTRIS L. A PORTENTOUS MEDICINAL PLANT EMPLOYING TISSUE CULTURE TECHNOLOGY

2017 ◽  
Vol 4 (2) ◽  
pp. 39-46
Author(s):  
Jamuna S ◽  
Anjali B ◽  
Karthika K
Keyword(s):  
Tissue Culture ◽  
Callus Induction ◽  
Root Elongation ◽  
Nodal Segments ◽  
Tribulus Terrestris ◽  
Ms Medium ◽  
Shoot Induction ◽  
Regenerated Plantlets ◽  
Juvenile Explants ◽  
Culture Technology

A protocol for micropropagation of Tribulus terrestris, an important medicinal herb was established using juvenile explants viz., leaf, node and internode. All the explants were tested for callus induction on Murashige and Skoog’s (MS) medium, supplemented with BAP, NAA and 2,4-D. Among the three explants leaf explant responded well (98%) for the callus induction in the MS medium composted with BAP and NAA (4.0 and 0.5 mg/L) followed by the nodal segments (58.75%) in the same medium. Maximum number of shoot induction from the callus of leaf derived explants (91.1%) was perceived on MS medium fortified with BAP 4.0 mg/L and NAA (0.5 mg/L). Moreover, root elongation and profuse rooting percentage (77.19%) were achieved when the well-grown shoots were cultured on MS media supplemented with IAA (2.0 mg/L) for leaf callus derived shoots. The regenerated plantlets were hardened and established at 80% survival rate in hardening media encompassed with red soil, sand and vermicompost in the ratio of 1:1:1 by volume.

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