Effects of the Srl5 allele for resistance on development of the stem rust fungus and cellular responses in wheat

1986 ◽  
Vol 64 (3) ◽  
pp. 626-631 ◽  
Author(s):  
H. D. M. Gousseau ◽  
B. J. Deverall
Keyword(s):  
Stem Rust ◽  
Rust Fungus ◽  
Cell Formation ◽  
Cellular Responses ◽  
Host Cells ◽  
Puccinia Graminis ◽  
Wheat Line ◽  
Cell Necrosis ◽  
Virulent Strains ◽  
Mother Cells

The development of avirulent and virulent strains of stem rust (Puccinia graminis Pers. f.sp. tritici Eriks. & Henn.) in a susceptible wheat line and two cultivars bearing the Sr15 allele for resistance was studied, mainly by fluorescence microscopy. Formation of appressoria, substomatal vesicles, infection hyphae, and the first haustorium was unaffected by resistance. The first effect of Sr15 expression was a slower rate of haustorial mother cell formation and was first seen 48 h after inoculation. Effects on hyphal branching and colony radii followed. Necrosis of host cells was first seen at 42 h, but inspection of individual infection sites showed that necrosis did not coincide with effects on haustorial mother cells. It is possible that deterioration of host cells leading to visible host cell necrosis may be related to effects on rust development. Sr15 expression gave a mesothetic reaction, first seen microscopically 60 h after inoculation. Differences between individual infection sites in this reaction may be related to the timing of the onset of necrosis.

Histological studies on host-cell necrosis conditioned by the Sr6 gene for resistance in wheat to stem rust

1977 ◽  
Vol 55 (11) ◽  
pp. 1445-1452 ◽  
Author(s):  
D. J. Samborski ◽  
W. K. Kim ◽  
R. Rohringer ◽  
N. K. Howes ◽  
R. J. Baker
Keyword(s):  
Triticum Aestivum ◽  
Stem Rust ◽  
Fungal Growth ◽  
Triticum Aestivum L ◽  
Host Cells ◽  
Puccinia Graminis ◽  
Near Isogenic Lines ◽  
Cell Necrosis ◽  
Calcofluor White ◽  
Histological Studies

Seedlings of resistant (Sr6) and susceptible (sr6) near-isogenic lines of wheat (Triticum aestivum L.) were inoculated with a race of stem rust (Puccinia graminis Pers. f. sp. tritici Eriks. & E. Henn.) that was avirulent on the line with Sr6 and they were kept at 19, 25, 26, and 27 °C. Fluorescence microscopy was used to detect autofluorescing necrotic host cells and rust colonies after these were stained with a fiuorochrome (Calcofluor White M2R New).In leaves containing the Sr6 gene, a smaller percentage of colonies grown at 25 °C had necrotic cells associated with them than those that were grown at 19 °C. The incidence of colony-associated necrosis in these leaves could be further reduced by increasing the temperature to 26 °C and 27 °C. Similarly, the number of necrotic host cells per colony decreased with an increase in temperature. Colonies in genotypically resistant leaves were usually smaller than those in genotypically susceptible leaves, but the differences in colony sizes between these two lines decreased at the higher temperatures.When infected plants containing the Sr6 gene were kept for varying times at 25 °C and then were transferred to 19 °C, there was significantly less fungal growth and more necrosis than in plants kept continuously at 25 °C. This necrosis occurred largely in those cells that were invaded after the transfer to 19 °C, when the Sr6 gene was activated.

THE PHYSIOLOGY OF HOST–PARASITE RELATIONS: XVIII. DISTRIBUTION OF TRITIUM-LABELLED CYTIDINE, URIDINE, AND LEUCINE IN WHEAT LEAVES INFECTED WITH THE STEM RUST FUNGUS

1967 ◽  
Vol 45 (5) ◽  
pp. 555-563 ◽  
Author(s):  
P. K. Bhattacharya ◽  
Michael Shaw
Keyword(s):  
Stem Rust ◽  
Rust Fungus ◽  
Host Cells ◽  
Fungal Mycelium ◽  
Puccinia Graminis ◽  
Mesophyll Cells ◽  
Wheat Leaves ◽  
Host Parasite

Wheat leaves were detached 6 days after inoculation with the stem rust fungus (Puccinia graminis var. tritici Erikss. and Henn.) and fed with tritiated leucine, cytidine, uridine, or thymidine. Mesophyll cells in infected zones incorporated more leucine into protein and more cytidine and uridine into RNA than did cells in adjacent uninfected tissue. Leucine, cytidine, and uridine were also heavily incorporated by fungal mycelium and developing uredospores. Grain counts over host nuclei in the infected zone were two to three-fold of those over nuclei in adjacent uninfected zones. There was no detectable incorporation of thymidinemethyl-3H into either the fungus or the host cells. The results are discussed.

Electron microscopy of susceptible and resistant near-isogenic (sr6/Sr6) lines of wheat infected by Puccinia graminis tritici. III. Ultrastructure of incompatible interact

1979 ◽  
Vol 57 (23) ◽  
pp. 2626-2634 ◽  
Author(s):  
D. E. Harder ◽  
D. J. Samborski ◽  
R. Rohringer ◽  
S. R. Rimmer ◽  
W. K. Kim ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Stem Rust ◽  
Stem Rust Resistance ◽  
Temperature Sensitive ◽  
Puccinia Graminis ◽  
Cell Necrosis ◽  
Mitochondrial Membranes ◽  
Wheat Stem Rust ◽  
Mesophyll Cells ◽  
Mother Cells

The interaction between avirulent wheat stem rust and wheat mesophyll cells containing the temperature-sensitive Sr6 gene for stem rust resistance was studied by electron microscopy. Mesophyll cells that were invaded at 26 °C (conditioning compatibility) did not develop any signs of incompatibility after they were transferred to 19 °C, at which temperature incompatibility is normally expressed. In host tissue that appeared to be invaded after the change from 26 to 19 °C, the early ultrastructural symptoms of incompatibility were a more electron-dense and often perforated invaginated host plasmalemma, disruptions of the noninvaginated host plasmalemma, vacuolation of the cytoplasm, and accumulations of electron-dense material along the membranes of the vacuoles. At later stages in the development of incompatible interactions, the electron-dense accumulations along the vacuole membranes increased in size and occurred along chloroplast and mitochondrial membranes. Eventually, the entire protoplasts were electron dense and collapsed. In haustoria and haustorial mother cells, incompatibility was usually expressed by a uniform increase in electron density of the cytoplasm. In the Sr6/P6 interaction at 19 °C, host cell necrosis was not always accompanied by fungal necrosis or vice versa. In Sr5/P5 interactions, which were examined for comparison, the intracellular symptoms of incompatibility were similar to those of the Sr6/P6 interactions.

Effect of blasticidin S, ethionine, and polyoxin D on stem rust development and host-cell necrosis in wheat near-isogenic for gene Sr6

1977 ◽  
Vol 55 (5) ◽  
pp. 568-573 ◽  
Author(s):  
W. K. Kim ◽  
R. Rohringer ◽  
D. J. Samborski ◽  
N. K. Howes
Keyword(s):  
Host Cell ◽  
Stem Rust ◽  
Fungal Growth ◽  
Triticum Aestivum L ◽  
Hypersensitive Reaction ◽  
Host Cells ◽  
Puccinia Graminis ◽  
Cell Necrosis ◽  
Blasticidin S ◽  
Wheat Leaves

Seedlings of resistant (Sr6) and susceptible (sr6) near-isogenic lines of wheat (Triticum aestivum L.) were inoculated with an avirulent (P6) race of stem rust (Puccinia graminis (Pers.) f.sp. tritici Eriks. & Henn.) and kept for 2 days at 26 °C where the Sr6 gene is ineffective, treated with blasticidin S, ethionine, polyoxin D, or buffer, and transferred to 19 °C where the Sr6 gene is normally effective. One and 2 days later, leaves were stained with Calcofluor and examined by fluorescence microscopy to detect autofluorescing necrotic host cells and Calcofluor-stained stem rust colonies.Blasticidin S was phytotoxic to wheat leaves at concentrations that had no effect on fungal growth during the first 2 days after treatment. At later stages, extensive host necrosis, resulting from the phytotoxicity of this antibiotic, inhibited rust development.Ethionine and polyoxin D strongly inhibited rust development at concentrations that were not phytotoxic. In genotypically resistant leaves treated with ethionine and polyoxin D there were fewer necrotic cells associated with stem rust colonies than in leaves treated with buffer. The spacial distribution of necrotic cells was consistent with the view that necrosis occurs only in cells newly invaded after the temperature was lowered to 19 °C.The observations do not support the concept that host-cell necrosis in the hypersensitive reaction conditioned by this gene results from the death of the fungus.

Components of resistance in barley to stem rust: pre- and post-penetration development in seedling and adult plant leaves

1996 ◽  
Vol 74 (8) ◽  
pp. 1305-1312 ◽  
Author(s):  
J. Q. Liu ◽  
D. E. Harder
Keyword(s):  
Stem Rust ◽  
Phenotypic Expression ◽  
Colony Growth ◽  
Host Cells ◽  
Adult Plant ◽  
Puccinia Graminis ◽  
Plant Leaves ◽  
Breeding Lines ◽  
Mother Cells ◽  
Different Levels

Seven barley (Hordeum vulgare) cultivars or breeding lines with different levels of resistance to stem rust and the susceptible wheat cultivar Little Club (Triticum aestivum) were inoculated with races QCC, QFC, and TPM of Puccinia graminis f.sp. tritici and examined using light microscopy to associate the sequence of histological events with phenotypic expression of resistance. There were no significant differences in urediniospore germination, appressorium formation, or substomatal penetration in any of the race–cultivar combinations in seedling leaves. Formation of primary haustorium mother cells (HMCs) was not affected by the presence or absence of the resistance gene Rpg1 when inoculated with race QCC, but was reduced considerably in lines possessing this gene after inoculation with races QFC and TPM. Development of all races was arrested during primary HMC formation to a greater extent in all barley lines than in Little Club wheat. The expression of resistance in barley to P. g. tritici was associated with the frequency of post-penetration abortion, the degree of reduction in colony growth, and the incidence of colony-associated necrosis of host cells. The rankings of the host lines based on these histological events were in agreement with the rankings for receptivity and urediniospore production determined previously on stem tissues of adult barley plants. Keywords: barley, stem rust, resistance, components, histology.

Haustoria-like branches in axenic cultures of Puccinia graminis f. sp. tritici

1969 ◽  
Vol 47 (11) ◽  
pp. 1816-1817 ◽  
Author(s):  
P. G. Williams
Keyword(s):  
Stem Rust ◽  
Rust Fungus ◽  
Puccinia Graminis ◽  
Wheat Stem Rust ◽  
Artificial Culture ◽  
Axenic Cultures

Hyphae of the wheat stem rust fungus form short, lateral projections under conditions of artificial culture that are unfavorable for saprophytic growth. It is suggested that the structures are homologous with the haustoria of intercellular rust mycelium.

scholarly journals The stem rust fungus Puccinia graminis f. sp. tritici induces centromeric small RNAs during late infection that direct genome-wide DNA methylation

2018 ◽  
Author(s):  
Jana Sperschneider ◽  
Ashley W. Jones ◽  
Jamila Nasim ◽  
Bo Xu ◽  
Silke Jacques ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Stem Rust ◽  
Small Rnas ◽  
Genome Stability ◽  
Cytosine Methylation ◽  
Rust Fungus ◽  
Epigenetic Silencing ◽  
Puccinia Graminis ◽  
Genome Wide ◽  
Late Wave

AbstractBackgroundSilencing of transposable elements (TEs) is essential for maintaining genome stability. Plants use small RNAs (sRNAs) to direct DNA methylation to TEs (RNA-directed DNA methylation; RdDM). Similar mechanisms of epigenetic silencing in the fungal kingdom have remained elusive.ResultsWe use sRNA sequencing and methylation data to gain insight into epigenetics in the dikaryotic fungus Puccinia graminis f. sp. tritici (Pgt), which causes the devastating stem rust disease on wheat. We use Hi-C data to define the Pgt centromeres and show that they are repeat-rich regions (∼250 kb) that are highly diverse in sequence between haplotypes and, like in plants, are enriched for young TEs. DNA cytosine methylation is particularly active at centromeres but also associated with genome-wide control of young TE insertions. Strikingly, over 90% of Pgt sRNAs and several RNAi genes are differentially expressed during infection. Pgt induces waves of functionally diversified sRNAs during infection. The early wave sRNAs are predominantly 21 nts with a 5’ uracil derived from genes. In contrast, the late wave sRNAs are mainly 22 nt sRNAs with a 5’ adenine and are strongly induced from centromeric regions. TEs that overlap with late wave sRNAs are more likely to be methylated, both inside and outside the centromeres, and methylated TEs exhibit a silencing effect on nearby genes.ConclusionsWe conclude that rust fungi use an epigenetic silencing pathway that resembles RdDM in plants. The Pgt RNAi machinery and sRNAs are under tight temporal control throughout infection and might ensure genome stability during sporulation.

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