Comportement in vitro de bourgeons axillaires de type indéterminé du palmier dattier (Phoenix dactylifera)

1990 ◽  
Vol 68 (9) ◽  
pp. 2004-2009 ◽  
Author(s):  
Nadia Bouguedoura ◽  
Nicole Michaux-Ferrière ◽  
Jean-Louis Bompar
Keyword(s):  
Floral Induction ◽  
Vegetative State ◽  
Phoenix Dactylifera ◽  
Axillary Buds ◽  
Indolebutyric Acid ◽  
Vegetative Buds ◽  
Murashige And Skoog's Medium ◽  
Murashige And Skoog’S Medium

Indeterminate axillary buds excised from young offshoots of date palm (Phoenix dactylifera L.) developed into flowering or vegetative buds when cultured under different in vitro conditions. Floral induction was observed in explants cultured in the dark on Murashige and Skoog's medium supplemented with 50 g∙L−1 of sucrose and several auxins and cytokinins in a ratio favouring the auxins. In contrast, vegetative buds were obtained from explants cultured under a 16-h photoperiod on Murashige and Skoog's medium supplemented with 30 g∙L−1 of sucrose and 1 mg∙L−1 of indolebutyric acid. The results showed that numerous vegetative meristems can be produced from indeterminate buds cultured in vitro. The results also confirmed the observations made during an in vivo study of flowering and vegetative bud development. The importance of the nutritional contribution of the leaves surrounding the flowering buds was pointed out. Key words: Phoenix dactylifera, axillary buds, indeterminate buds, in vitro culture, floral state, vegetative state, morphogenesis.

Evaluation of anti-diabetic and anti-tumoral activities of bioactive compounds from Phoenix dactylifera L’s leaf: In vitro and in vivo approach

2016 ◽  
Vol 84 ◽  
pp. 415-422 ◽  
Author(s):  
Mouna Chakroun ◽  
Bassem Khemakhem ◽  
Hazem Ben Mabrouk ◽  
Hanen El Abed ◽  
Mohamed Makni ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Phoenix Dactylifera

Conditions d'apparition d'une embryogénèse somatique sur des cals issus de la culture de tissus foliaires du chêne vert (Quercus ilex)

1989 ◽  
Vol 67 (4) ◽  
pp. 1066-1070 ◽  
Author(s):  
C. Féraud-Keller ◽  
H. Espagnac
Keyword(s):  
Acetic Acid ◽  
Somatic Embryos ◽  
Quercus Ilex ◽  
Foliar Tissue ◽  
Murashige And Skoog's Medium ◽  
Old Trees ◽  
Murashige And Skoog’S Medium

The proliferation of parenchymatous foliar tissue of Quercus ilex was obtained from current year leaves taken from old trees and cultivated in vitro on modified Murashige and Skoog's medium supplemented with benzylaminopurine (4 mg ∙ L−1) and naphthyl acetic acid (0.5 mg ∙ L−1). Only the fragments cultured in October reacted. The neoformations only appeared on calluses that had not been subcultured for 7 months. Primary nodules arising on these calluses were removed and subcultured on the same medium either in the dark or in the light. In the dark only, they produced secondary nodules, which were the source of somatic embryos both in light and dark. Presently, they seem to regulate their structure in the dark but they do not develop in a way that leads to germination.

Floral induction in date palm seedlings (Phoenix dactylifera var. Deglet Nour) cultured in vitro

1987 ◽  
Vol 65 (1) ◽  
pp. 137-142 ◽  
Author(s):  
Saïda Ammar ◽  
Abdellatif Benbadis ◽  
Bal Krishna Tripathi
Keyword(s):  
Date Palm ◽  
Root Formation ◽  
Early Stage ◽  
Floral Induction ◽  
Phoenix Dactylifera ◽  
Flower Bud ◽  
Precocious Flowering ◽  
Cultural Conditions ◽  
Bud Initiation

Flower bud initiation in 5-month-old seedlings of date palm (Phoenix dactylifera L. var. Deglet Nour) was studied under controlled conditions. Normally inflorescence formation in mature plants takes 8 to 10 years. In juvenile plants inflorescence formation was induced in a 16-h day at 28 °C, by a combination of 6-benzylaminopurine, indoleacetic acid, and glucose or sucrose. The present investigation has determined favourable cultural conditions for floral induction in date palm in vitro at a very early stage of ontogeny. Both male and female flowers were induced on young plants. Floral induction usually occurred only when root formation was completely inhibited. The apparent antagonism between root formation and floral development suggests a possible competition in the young plant for growth substances, although production of floral inhibitory substances from the root cannot be precluded. These observations on the induction of precocious flowering in date palm seedlings suggest a model of development, corresponding to neoteny, of this tree as an herb.

scholarly journals Antileishmanial Activity of Date (Phoenix dactylifera L) Fruit and Pit Extracts In Vitro

2016 ◽  
Vol 21 (4) ◽  
pp. NP98-NP102 ◽  
Author(s):  
Sedighe Albakhit ◽  
Shahram Khademvatan ◽  
Monir Doudi ◽  
Masoud Foroutan-Rad
Keyword(s):  
Leishmania Major ◽  
Morphological Changes ◽  
Public Health Problem ◽  
Phoenix Dactylifera ◽  
Antileishmanial Activity ◽  
Major Public Health Problem ◽  
Methanolic Extracts ◽  
Phoenix Dactylifera L

Leishmaniasis is considered as a major public health problem worldwide. Current drugs in treatment of leishmaniasis have some limitations; thus, the current study was aimed to assess the methanolic extracts of pit and fruit of Phoenix dactylifera against Leishmania major promastigotes. L major promastigotes were cultured in RPMI 1640 and incubated at 25°C ± 1°C for 24, 48, and 72 hours. For obtaining the IC50 (half maximal inhibitory concentration) value, MTT assay was employed. Furthermore, promastigotes were examined in terms of morphology under light microscope. About 48 hours after treatment, IC50s were estimated 23 μg/mL and 500 mg/mL for methanolic extracts of pit and fruit of P dactylifera, respectively. Both extracts exhibited a dose and time-dependent antileishmanial activity against L major parasites. Also, some visible morphological changes were seen. This finding revealed both date fruit and pit, are effective against L major promastigotes. Further studies should be designed in future based on apoptosis induction in vitro and in vivo.

scholarly journals Micropropagation, genetic fidelity assessment and phytochemical studies of Clerodendrum thomsoniae Balf. f. with special reference to its anti-stress properties

2019 ◽  
pp. 09-15 ◽  
Author(s):  
Pallab Kar ◽  
Arnab Kumar Chakraborty ◽  
Malay Bhattacharya ◽  
Tanmayee Mishra ◽  
Arnab Sen
Keyword(s):  
Special Reference ◽  
Genetic Fidelity ◽  
Fidelity Assessment ◽  
Callus Regeneration ◽  
Murashige And Skoog's Medium ◽  
Murashige And Skoog’S Medium ◽  
Phytochemical Studies ◽  
New Crop ◽  
Nodal Culture

Clerodendrum thomsoniae commonly known as bleeding heart vine or bag flower which is a good candidate for a new crop for the floriculture industry. In this study, in-vitro callus regeneration of C. thomsoniae through nodal culture has been attempted. Murashige and Skoog’s medium supplemented with 2 mg/l BAP and 0.5 mg/l

scholarly journals A Review on Phyto-pharmacological Significance of Ajwa pits (Phoenix dactylifera L.)

2020 ◽  
Vol 2 (3) ◽  
pp. 26-45
Author(s):  
Anam Amir ◽  
Imama Ansari ◽  
Fasiha Arif ◽  
Sughra Bano ◽  
Fariha Amir ◽  
...  
Keyword(s):  
Antimicrobial Agents ◽  
Phoenix Dactylifera ◽  
Review Article ◽  
Life Stages ◽  
Frequent Use ◽  
Phoenix Dactylifera L ◽  
Pharmacological Significance ◽  
Desert Areas

Phoenix dactylifera L. (Ajwa date) is a natural plant that belongs to the Arecaceae family and consumed as a fruit. It is cultured all over the world especially in desert areas like Arab regions and has nutritional significance and pharmacological values. P. dactylifera L. dominates itself from other dates because of its enrichment in dietary fibers, vitamins, minerals and sugars. The study exhibits that P. dactylifera L. contains certain phytochemicals including phenols and flavonoids which act as antimicrobial agents. The distinctive profile of phytochemicals in P. dactylifera L.is the reason for its frequent use as a medicinal plant. In vivo and in vitro studies have been performed to understand the complete nature of P. dactylifera L. This review article gives an overview of the nutritional significance, pharmacological actions, life stages and phytochemical mechanism of P. dactylifera L.

scholarly journals Sustained In Vitro and In Vivo Delivery of Metformin from Plant Pollen-Derived Composite Microcapsules

Pharmaceutics ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1048
Author(s):  
Noha M. Meligi ◽  
Amro K.F. Dyab ◽  
Vesselin N. Paunov
Keyword(s):  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Phoenix Dactylifera ◽  
Relative Bioavailability ◽  
Diabetic Rats ◽  
In Vivo Studies ◽  
Pharmacokinetic Parameters ◽  
Sprague Dawley

We developed a dual microencapsulation platform for the type 2 diabetes drug metformin (MTF), which is aimed to increase its bioavailability. We report the use of Lycopodium clavatum sporopollenin (LCS), derived from their natural spores, and raw Phoenix dactylifera L. (date palm) pollens (DPP) for MTF microencapsulation. MTF was loaded into LCS and DPP via a vacuum and a novel method of hydration-induced swelling. The loading capacity (LC) and encapsulation efficiency (EE) percentages for MTF-loaded LCS and MTF-loaded DPP microcapsules were 14.9% ± 0.7, 29.8 ± 0.8, and 15.2% ± 0.7, 30.3 ± 1.0, respectively. The release of MTF from MTF-loaded LCS microcapsules was additionally controlled by re-encapsulating the loaded microcapsules into calcium alginate (ALG) microbeads via ionotropic gelation, where the release of MTF was found to be significantly slower and pH-dependent. The pharmacokinetic parameters, obtained from the in vivo study, revealed that the relative bioavailability of the MTF-loaded LCS-ALG beads was 1.215 times higher compared to pure MTF, following oral administration of a single dose equivalent to 25 mg/kg body weight MTF to streptozotocin (STZ)-induced diabetic male Sprague-Dawley rats. Significant hypoglycemic effect was obtained for STZ-induced diabetic rats orally treated with MTF-loaded LCS-ALG beads compared to control diabetic rats. Over a period of 29 days, the STZ-induced diabetic rats treated with MTF-loaded LCS-ALG beads showed a decrease in the aspartate aminotransferase (AST), alanine aminotransferase (ALT), triglycerides, cholesterol, and low-density lipoprotein-cholesterol (LDL-C) levels, as well as an increase in glutathione peroxidase (GPx) and a recovery in the oxidative stress biomarker, lipid peroxidation (LPx). In addition, histopathological studies of liver, pancreas, kidney, and testes suggested that MTF-loaded LCS-ALG beads improved the degenerative changes in organs of diabetic rats. The LCS-ALG platform for dual encapsulation of MTF achieved sustained MTF delivery and enhancement of bioavailability, as well as the improved biochemical and histopathological characteristics in in vivo studies, opening many other intriguing applications in sustained drug delivery.

scholarly journals Pathogenicity and chemical control of Alternaria sp. on date palm (Phoenix dactylifera L.)

2021 ◽  
Vol 8 (2) ◽  
pp. 386-391
Author(s):  
Abdulnabi Abbdul Ameer Matrood ◽  
Claudia Adriana Ramírez Valdespino ◽  
Muhannad Abdulridha Al-Waeli ◽  
Mohammad Imad Khrieba ◽  
Abdelhak Rhouma
Keyword(s):  
Chemical Control ◽  
Date Palm ◽  
Management Strategies ◽  
Severity Index ◽  
Phoenix Dactylifera ◽  
Wide Range ◽  
Alternaria Sp ◽  
Fusarium Sp

Recently, a wide range of symptoms including light yellow lesions gradually turning into brown stripes were noticed on date palm leaves in Iraq. In this context, the aim of this study were to isolate the phytopathogens associated with these symptoms, evaluate their pathogenicity and assess the efficacy of two fungicides (Score and Pentanol) under in vitro and in vivo conditions. Two fungal species (Alternaria sp. and Fusarium sp.) were isolated from the symptomatic leaves of date palm. The results of pathogenicity tested proved the ability of Alternaria sp. inoculated separately or in combination with Fusarium sp. to infect the leaves of date palm trees with disease severity index (DSI) values of 67.33% and 65.99%, respectively. The effect of Score (88.76%) and Pentanol (82.91%) against Alternaria sp. was examined by poisoned food technique, which leads a significant increase in mycelial growth inhibition (for 300% of commercial recommended dose of fungicide). Test results indicate that prophylactic spraying of date palm leaves with Score or Pentanol effectively controlled Alternaria sp. with DSI values of 22.65% and 17.87%, respectively. To control Alternaria sp. in field within integrated pest management strategies, chemical control using Score or Pentanol should be taken in consideration.

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