scholarly journals HIDE AND SEEK: MOLECULAR BARCODING CLARIFIES THE DISTRIBUTION OF TWO CRYPTIC DUCKWEED SPECIES ACROSS ALBERTA

Botany ◽  
2021 ◽  
Author(s):  
Kanishka M. Senevirathna ◽  
Varina E. Crisfield ◽  
Theresa M. Burg ◽  
Robert A. Laird
Keyword(s):  
Cryptic Species ◽  
Dna Barcoding ◽  
Molecular Identification ◽  
Lemna Minor ◽  
Rapid Identification ◽  
Specific Primers ◽  
Molecular Barcoding ◽  
Geographic Ranges ◽  
Global Biodiversity ◽  
Species Specific

Regional and global biodiversity may be underestimated due to the presence of cryptic species: species that are morphologically similar, but genetically distinct. Here we focus on two cryptic duckweed species, Lemna minor and L. turionifera, which have overlapping geographic ranges and are easily mistaken for one another. We developed species-specific primers based on DNA barcoding sequences to facilitate the rapid identification of these two monomorphic duckweeds, allowing us to investigate their presence and distribution in Alberta, Canada. While current reports indicate the presence of L. turionifera (and the morphologically distinct L. trisulca) in Alberta, our data indicate that L. minor is also present, predominantly in the southern part of the province. Thus, this paper (1) contributes to the accuracy and completeness of a regional flora, and (2) provides useful and flexible tools for the rapid molecular identification of cryptic Lemna species, species of wide interest in such diverse fields as biotechnology, toxicology, bioremediation, and ecology.

scholarly journals Benchmarking DNA barcodes: an assessment using available primate sequences

Genome ◽  
2006 ◽  
Vol 49 (7) ◽  
pp. 851-854 ◽  
Author(s):  
Mehrdad Hajibabaei ◽  
Gregory AC Singer ◽  
Donal A Hickey
Keyword(s):  
New Species ◽  
Cryptic Species ◽  
Dna Barcoding ◽  
Species Identification ◽  
Dna Sequences ◽  
Phylogenetic Relationships ◽  
Primate Species ◽  
Dna Barcodes ◽  
Global Biodiversity ◽  
Efficient Sequence

DNA barcoding has been recently promoted as a method for both assigning specimens to known species and for discovering new and cryptic species. Here we test both the potential and the limitations of DNA barcodes by analysing a group of well-studied organisms—the primates. Our results show that DNA barcodes provide enough information to efficiently identify and delineate primate species, but that they cannot reliably uncover many of the deeper phylogenetic relationships. Our conclusion is that these short DNA sequences do not contain enough information to build reliable molecular phylogenies or define new species, but that they can provide efficient sequence tags for assigning unknown specimens to known species. As such, DNA barcoding provides enormous potential for use in global biodiversity studies.Key words: DNA barcoding, species identification, primate, biodiversity.

scholarly journals Rapid identification of the invasive fall armyworm Spodoptera frugiperda (Lepidoptera, Noctuidae) using species-specific primers in multiplex PCR

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Cheng-Lung Tsai ◽  
I.-Hsuan Chu ◽  
Ming-Hsun Chou ◽  
Theeraphap Chareonviriyaphap ◽  
Ming-Yao Chiang ◽  
...  
Keyword(s):  
Spodoptera Frugiperda ◽  
Egg Production ◽  
Mainland China ◽  
Fall Armyworm ◽  
The United States ◽  
Rapid Identification ◽  
Economic Losses ◽  
Specific Primers ◽  
Wide Range ◽  
Species Specific

Abstract The fall armyworm (FAW), Spodoptera frugiperda (Smith), is a major pest native to the Americas. A recent invasion of FAWs from Africa eastward to South Asia, the Indochina Peninsula, and mainland China has received much attention due to the considerable economic losses in agriculture. FAWs can rapidly colonise a new area, likely due to the wide range of host plants, good flying capability, and high egg production. Therefore, a convenient, quick, and accurate tool for FAW identification is urgently required to establish a FAW invasion management strategy. In this study, FAW-specific primers were designed to recognise FAWs on the basis of internal transcribed spacer 1 (ITS1). The results revealed the accurate FAW recognition of the three congeneric species and eight common corn lepidopteran pests, especially at their larval stage. Furthermore, species-specific primers have confirmed their efficacy by using 69 FAW specimens from Taiwan, Thailand, and the United States, with a 96% success rate, excluding 3 decayed specimens. By using the simple, reliable, and convenient FAW-specific primers, a pest management programme can be developed not only to reduce sequencing costs and experimental time from 2 days to 4 h, but eradicate the FAW as soon as it enters a new area.

Molecular identification of endangered Korean lady’s slipper orchids (Cypripedium, Orchidaceae) and related taxa

Botany ◽  
2015 ◽  
Vol 93 (9) ◽  
pp. 603-610 ◽  
Author(s):  
Jung Sung Kim ◽  
Hyoung Tae Kim ◽  
Sung-Won Son ◽  
Joo-Hwan Kim
Keyword(s):  
Endangered Species ◽  
Molecular Identification ◽  
Sequence Variation ◽  
Identification System ◽  
Terrestrial Orchid ◽  
Specific Primers ◽  
Plant Populations ◽  
Large Deletions ◽  
Endemic Taxon ◽  
Species Specific

Reliable and accurate species identification is essential to establish a strategy for monitoring and manipulation of plant populations for conservation biology. However, identification of nonflowering plants is difficult. Based on recent advances in molecular biological techniques, various molecular identification methodologies have been proposed and applied in plant science, with a focus on medicinally valuable species. Lady’s slipper orchids (Cypripedium) are a representative terrestrial orchid group that includes many endangered species. Of 46 species, 3 (Cypripedium japonicum Thunb., Cypripedium macranthos Sw., and Cypripedium guttatum var. koreanum Nakai) were reported from Korea and categorised as endangered species or at risk of becoming endangered because of the rapidly decreasing population. We generated an accurate molecular identification system for these species using the sequence variation and species-specific SNPs of two plastid loci, rpoC2 and the IGS region between atpF and atpH. We selected the atpF-H region for molecular identification of the Korean Cypripedium and related Taiwan-endemic taxon Cypripedium formosanum Hayata. Cypripedium guttatum var. koreanum contained large deletions of over 300 bp, and could be distinguished by electrophoresis. For the other three species, we designed new primers based on a specific insertion (C. macranthos) and SNPs (C. japonicum and C. formosanum). We confirmed that molecular identification enables detection of each species using species-specific primers.

Development of species-specific primers for rapid identification of Debaryomyces hansenii

2015 ◽  
Vol 193 ◽  
pp. 109-113 ◽  
Author(s):  
Petra Wrent ◽  
Eva-María Rivas ◽  
Elena Gil de Prado ◽  
José M. Peinado ◽  
María-Isabel de Silóniz
Keyword(s):  
Debaryomyces Hansenii ◽  
Rapid Identification ◽  
Specific Primers ◽  
Species Specific

Rapid identification of Saccharomonospora strains by multiplex PCR using species-specific primers within the 16S rRNA gene

1996 ◽  
Vol 27 (1) ◽  
pp. 89-95 ◽  
Author(s):  
Jung-Hoon Yoon ◽  
Sung Taik Lee ◽  
Yong Kook Shin ◽  
Sam-Bong Kim ◽  
Hong-Joong Kim ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Multiplex Pcr ◽  
Rapid Identification ◽  
Rrna Gene ◽  
Specific Primers ◽  
Species Specific ◽  
The 16S Rrna Gene
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