Rhizosphere bacteria ofCostulariaspp. from ultramafic soils in New Caledonia: diversity, tolerance to extreme edaphic conditions, and role in plant growth and mineral nutrition

2013 ◽  
Vol 59 (3) ◽  
pp. 164-174 ◽  
Author(s):  
Mathieu Gonin ◽  
Simon Gensous ◽  
Alexandre Lagrange ◽  
Marc Ducousso ◽  
Hamid Amir ◽  
...  
Keyword(s):  
Plant Growth ◽  
New Caledonia ◽  
Acc Deaminase ◽  
Rhizosphere Bacteria ◽  
Shoot Biomass ◽  
Ultramafic Soils ◽  
Edaphic Conditions ◽  
Colony Forming Units ◽  
Plant Growth Promoting ◽  
Calcium Magnesium

Rhizosphere bacteria were isolated from Costularia spp., pioneer sedges from ultramafic soils in New Caledonia, which is a hotspot of biodiversity in the South Pacific. Genus identification, ability to tolerate edaphic constraints, and plant-growth-promoting (PGP) properties were analysed. We found that 105colony-forming units per gram of root were dominated by Proteobacteria (69%) and comprised 21 genera, including Burkholderia (28%), Curtobacterium (15%), Bradyrhizobium (9%), Sphingomonas (8%), Rhizobium (7%), and Bacillus (5%). High proportions of bacteria tolerated many elements of the extreme edaphic conditions: 82% tolerated 100 μmol·L–1chromium, 70% 1 mmol·L–1nickel, 63% 10 mmol·L–1manganese, 24% 1 mmol·L–1cobalt, and 42% an unbalanced calcium/magnesium ratio (1/16). These strains also exhibited multiple PGP properties, including the ability to produce ammonia (65%), indole-3-acetic acid (60%), siderophores (52%), and 1-aminocyclopropane-1-carboxylate (ACC) deaminase (39%); as well as the capacity to solubilize phosphates (19%). The best-performing strains were inoculated with Sorghum sp. grown on ultramafic substrate. Three strains significantly enhanced the shoot biomass by up to 33%. The most successful strains influenced plant nutrition through the mobilization of metals in roots and a reduction of metal transfer to shoots. These results suggest a key role of these bacteria in plant growth, nutrition, and adaptation to the ultramafic constraints.

scholarly journals Morphological and Metabolite Responses of Potatoes under Various Phosphorus Levels and Their Amelioration by Plant Growth-Promoting Rhizobacteria

2021 ◽  
Vol 22 (10) ◽  
pp. 5162
Author(s):  
Leangsrun Chea ◽  
Birgit Pfeiffer ◽  
Dominik Schneider ◽  
Rolf Daniel ◽  
Elke Pawelzik ◽  
...  
Keyword(s):  
Amino Acids ◽  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Shoot Biomass ◽  
Root Surface Area ◽  
Limiting Factor ◽  
P Availability ◽  
P Deficiency ◽  
Plant Growth Promoting ◽  
Growth Promoting

Low phosphorus (P) availability is a major limiting factor for potatoes. P fertilizer is applied to enhance P availability; however, it may become toxic when plants accumulate at high concentrations. Therefore, it is necessary to gain more knowledge of the morphological and biochemical processes associated with P deficiency and toxicity for potatoes, as well as to explore an alternative approach to ameliorate the P deficiency condition. A comprehensive study was conducted (I) to assess plant morphology, mineral allocation, and metabolites of potatoes in response to P deficiency and toxicity; and (II) to evaluate the potency of plant growth-promoting rhizobacteria (PGPR) in improving plant biomass, P uptake, and metabolites at low P levels. The results revealed a reduction in plant height and biomass 60–80% under P deficiency compared to P optimum. P deficiency and toxicity conditions also altered the mineral concentration and allocation in plants due to nutrient imbalance. The stress induced by both P deficiency and toxicity was evident from an accumulation of proline and total free amino acids in young leaves and roots. Furthermore, root metabolite profiling revealed that P deficiency reduced sugars by 50–80% and organic acids by 20–90%, but increased amino acids by 1.5–14.8 times. However, the effect of P toxicity on metabolic changes in roots was less pronounced. Under P deficiency, PGPR significantly improved the root and shoot biomass, total root length, and root surface area by 32–45%. This finding suggests the potency of PGPR inoculation to increase potato plant tolerance under P deficiency.

scholarly journals Non rhizobial endophytic bacteria from chickpea (Cicer arietinum L.) tissues and their antagonistic traits

2019 ◽  
Vol 11 (2) ◽  
pp. 346-351
Author(s):  
Deepika Chhabra ◽  
Poonam Sharma
Keyword(s):  
Plant Growth ◽  
Indole Acetic Acid ◽  
Acc Deaminase ◽  
Bacterial Isolates ◽  
Cicer Arietinum L ◽  
Fluorescent Pigment ◽  
Biocontrol Activity ◽  
Plant Growth Promoting ◽  
Gibberelic Acid ◽  
Growth Promoting

Bacteria that colonize plant tissues other than rhizobia and are beneficial for plant growth referred to non rhizobial plant growth-promoting endophytic bacteria (PGPEB). This study was designed to assay the biocontrol activity of plant growth promoting endophytic bacterial isolates those found positive for P. solubilization, ACC deaminase, Indole acetic acid and Gibberelic acid production. These bacterial isolates were obtained from chickpea (Cicer arietinum L.) tissues (roots and nodules).  In a previous study a total of 263 non rhizobial endophytic bacterial isolates were isolated. Out of 263 isolates, 64.5% and 34.5% were Gram positive and negative, respectively. Further for biochemical characterization, catalase, oxidase, citrate utilization, nitrate reduction, methyl red and Voges Proskauer’s tests, were performed. On the basis of P solubilization, ACC deaminase, Indole acetic acid and Gibberelic acid production 75 potential isolates were selected and screened for their biocontrol activity viz. (production of cell wall degrading enzymes, production of HCN and fluorescent pigment). Out of 75 isolates, only 29 isolates produced cellulase, 64 isolates were able to produce protease and 28 were positive for both cellulose and protease. Of 75 endophytic isolates 12 isolates (7 from root tissue and 5 from nodules tissue, respectively) were positive for HCN production and 16 isolates were found to be fluorescent pigment producer under µv ligh. As chemical fertilizers and pesticides have detrimental effects on the environment. So these bacterial endophytic isolates will be used not only as a biofertilizer because of their plant growth promotional activities but also used as an alternative of synthetic chemicals for control of several plant diseases.

ACC deaminase from plant growth-promoting bacteria affects crown gall development

2007 ◽  
Vol 53 (12) ◽  
pp. 1291-1299 ◽  
Author(s):  
Youai Hao ◽  
Trevor C. Charles ◽  
Bernard R. Glick
Keyword(s):  
Plant Growth ◽  
Castor Bean ◽  
Crown Gall ◽  
Acc Deaminase ◽  
Plant Growth Promoting Bacteria ◽  
Bean Plants ◽  
Tumour Formation ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Agrobacterium Tumefaciens C58

In addition to the well-known roles of indoleacetic acid and cytokinin in crown gall formation, the plant hormone ethylene also plays an important role in this process. Many plant growth-promoting bacteria (PGPB) encode the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase, which can degrade ACC, the immediate precursor of ethylene in plants, to α-ketobutyrate and ammonia and thereby lower plant ethylene levels. To study the effect of ACC deaminase on crown gall development, an ACC deaminase gene from the PGPB Pseudomonas putida UW4 was introduced into Agrobacterium tumefaciens C58, so that the effect of ACC deaminase activity on tumour formation in tomato and castor bean plants could be assessed. Plants were also coinoculated with A. tumefaciens C58 and P. putida UW4 or P. putida UW4-acdS– (an ACC deaminase minus mutant strain). In both types of experiments, it was observed that the presence of ACC deaminase generally inhibited tumour development on both tomato and castor bean plants.

scholarly journals Characterization and optimization of 1-Aminocyclopropane-1-Carboxylate Deaminase (ACCD) activity in different rhizospheric PGPR along with Microbacterium sp. strain ECI-12A

2013 ◽  
Vol 1 (1) ◽  
pp. 11-15 ◽  
Author(s):  
Umesh P. Shrivastava ◽  
Ashok Kumar
Keyword(s):  
Plant Growth ◽  
Pcr Amplification ◽  
Acc Deaminase ◽  
Plant Growth Promoting Rhizobacteria ◽  
Optimum Activity ◽  
Rice Rhizosphere ◽  
Acds Gene ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Culture Growth

A total of nine strains of plant growth promoting rhizobacteria were analyzed for ACC deaminase activity, where highest ACC deaminase activity was found in Klebsiella sp strain ECI-10A (539.1 nmol α-keto butyrate/ mg protein/ h) and lowest in Microbacterium sp strain ECI-12A (122.0 nmol α-keto butyrate/ mg protein/ h). Although Microbacterium sp strain ECI-12A showed lowest level of ACC deaminase activity, but, the species of Microbacterium isolated from rhizosphere is the first report. Microbacterium sp strain ECI-12A was also analyzed under varying conditions of time, amount of 1-Aminocyclopropane-1- carboxylate (ACC), and temperature for optimization of the ACC deaminase activity. The optimum activity was recorded with the supplementation of 5mM ACC at 30°C temperature after 24h of culture growth. All the nine strains showed acdS gene in the PCR amplification of that gene. No any rhizospheric Microbacterium species showing ACC deaminase activity have been reported earlier, therefore, we report here ACC deaminase activity in Microbacterium sp ECI-12A isolated from rice rhizosphere is a novel finding. DOI: http://dx.doi.org/10.3126/ijasbt.v1i1.7921 Int J Appl Sci Biotechnol, 2013, Vol. 1(1): 11-15

scholarly journals Exploring the Multi-trait Plant Growth Promotion Capability of Commercial Liquid Biofertilizers Isolates

2015 ◽  
Vol 9 (3) ◽  
pp. 24-37 ◽  
Author(s):  
Mohammed Faisal Ansari ◽  
Devayani R. Tipre ◽  
Shailesh R. Dave
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Phosphate Solubilization ◽  
Growth Promotion ◽  
Acc Deaminase ◽  
Multiple Traits ◽  
The Past ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Field Efficiency

Organic farming is gaining popularity where bio-inoculants could play a key role in promoting the growth of plants. The liquid biofertilizers concept is new to farmers and developed recently. Lots of liquid biofertilizers formulations and field efficiency were shown in the past by various researchers, but the plant growth promoting (PGP) efficiency of the liquid biofertilizers isolates were not reported till date. In the present work 6 different commercially available liquid biofertilizers were used to isolate the organism. These isolated cultures were used to study their PGP efficiency with respect to phosphate solubilization and production of EPS, IAA, siderophore, ammonia, chitinase, ACC-deaminase and HCN. The phosphate solubilization was shown up to 303 g/ml by APS isolate. EPS production was shown by using different C sources and production up to 24 g/l was shown by studied isolated. Most of the organisms studied were able to produce IAA and highest production was shown up to 20 g/ml. More than 65% studied isolates showed siderophore and ACC-deaminase production. The present study shows that the commercial liquid biofertilizer isolates possess multiple traits of plant growth promotion. DOI: http://dx.doi.org/10.3126/ijls.v9i3.12463   International Journal of Life Sciences 9 (3): 2015; 24-37

scholarly journals Assessment of the Capacity of Beneficial Bacterial Inoculants to Enhance Canola (Brassica napus L.) Growth under Low Water Activity

Agronomy ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1449
Author(s):  
Dasun Premachandra ◽  
Lee Hudek ◽  
Aydin Enez ◽  
Ross Ballard ◽  
Steve Barnett ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Plant Growth ◽  
Water Activity ◽  
Production Costs ◽  
Annual Rainfall ◽  
Shoot Biomass ◽  
Brassica Napus L ◽  
Environmentally Sustainable ◽  
Plant Growth Promoting ◽  
Growth Promoting

Canola (Brassica napus L.) is the third largest crop produced in Australia after wheat and barley. For such crops, the variability of water access, reduced long-term annual rainfall and increasing water prices, higher overall production costs, and variability in production quantity and quality are driving the exploration of new tools to maintain production in an economical and environmentally sustainable way. Microorganisms associated with the rhizosphere have been shown to enhance plant growth and offer a potential way to maintain or even increase crop production quality and yield in an environmentally sustainable way. Here, seven bacterial isolates from canola rhizosphere samples are shown to enhance canola growth, particularly in low water activity systems. The seven strains all possessed commonly described plant growth promoting traits, including the ability to produce indole-3-acetic acid and 1-aminocyclopropane-1-carboxylate deaminase, and the capacity to solubilise nutrients (Fe2+/3+ and PO43−). When the isolates were inoculated at the time of sowing in pot-based systems with either sand or clay loam media, and in field trials, a significant increase in dry root and shoot biomass was recorded compared to uninoculated controls. It is likely that the strains’ plant growth promoting capacity under water stress is due to the combined effects of the bacterial phenotypes examined here.

Azospirillum– plant relationships: environmental and physiological advances (1990–1996)

1997 ◽  
Vol 43 (2) ◽  
pp. 103-121 ◽  
Author(s):  
Yoav Bashan ◽  
Gina Holguin
Keyword(s):  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Comprehensive Analysis ◽  
Rhizosphere Bacteria ◽  
Bacterial Inoculation ◽  
Previous Review ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Physiological Studies

This review presents a critical and comprehensive analysis of the developments in environmental and physiological studies related to Azospirillum interactions with plants based on information published between 1990 and 1996. It was designed as an update of a previous review with a similar scope. Apart from an update, this review emphasizes the central issues of Azospirillum research today, such as coinoculation with other microorganisms and hormonal studies, shows the less researched areas, and proposes possible avenues for the exploitation of this bacterium in areas other than agriculture.Key words: Azospirillum, bacterial inoculation, plant–bacteria interaction, plant growth promoting rhizobacteria, rhizosphere bacteria.

Levels of ACC and related compounds in exudate and extracts of canola seeds treated with ACC deaminase-containing plant growth-promoting bacteria

2001 ◽  
Vol 47 (4) ◽  
pp. 368-372 ◽  
Author(s):  
Donna M Penrose ◽  
Bernard R Glick
Keyword(s):  
Plant Growth ◽  
Acc Deaminase ◽  
Aminobutyric Acid ◽  
Plant Growth Promoting Bacteria ◽  
Canola Seed ◽  
Test Part ◽  
Seed Exudate ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Related Compounds

It was previously proposed that plant growth-promoting bacteria that possess 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase could utilize ACC that is present in the exudate of germinating canola seeds. The uptake and cleavage of ACC by these bacteria would lower the level of ACC, and thus ethylene within the plant, and reduce the extent of its inhibition on root elongation. To test part of the above mentioned model, ACC levels were monitored in canola seed tissues and exudate during germination. Lower amounts of ACC were present in the exudate and tissues of seeds treated with the plant growth-promoting bacterium Enterobacter cloacae CAL3, than in control seeds treated with MgSO4. The ACC-related compounds, α- and γ-aminobutyric acids, both known to stimulate ethylene production, were also measured in the canola seed exudate and tissues. Approximately the same levels of α-aminobutyric acid were present in the exudates of the bacterium-treated seeds and the control seeds, but the amount of α-aminobutyric acid was lower in the tissues of the bacterium-treated seeds than in the control seeds. Smaller quantities of γ-aminobutyric acid were seen in both the exudate and tissues of the E. cloacae CAL3-treated seeds than in the control seeds.Key words: ACC ethylene, canola, seed extract, seed exudate, plant growth-promoting bacteria.

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