scholarly journals Influence of wave action on the partitioning and transport of unattached and floc-associated bacteria in fresh water

2015 ◽  
Vol 61 (8) ◽  
pp. 584-596 ◽  
Author(s):  
Andrew J. Sousa ◽  
Ian G. Droppo ◽  
Steven N. Liss ◽  
Lesley Warren ◽  
Gideon Wolfaardt

The dynamic interaction of bacteria within bed sediment and suspended sediment (i.e., floc) in a wave-dominated beach environment was assessed using a laboratory wave flume. The influence of shear stress (wave energy) on bacterial concentrations and on the partitioning and transport of unattached and floc-associated bacteria was investigated. The study showed that increasing wave energy (0.60 and 5.35 N/s) resulted in a 0.5 to 1.5 log increase in unattached cells of the test bacterium Pseudomonas sp. strain CTO7::gfp-2 in the water column. There was a positive correlation between the bacterial concentrations in water and the total suspended solids, with the latter increasing from values of near 0 to up to 200 mg/L over the same wave energy increase. The median equivalent spherical diameter of flocs in suspension also increased by an order of magnitude in all experimental trials. Under both low (0.60 N/s) and high (5.35 N/s) energy regime, bacteria were shown to preferentially associate with flocs upon cessation of wave activity. The results suggest that collecting water samples during periods of low wave action for the purpose of monitoring the microbiological quality of water may underestimate bacterial concentrations partly because of an inability to account for the effect of shear stress on the erosion and mobilization of bacteria from bed sediment to the water column. This highlights the need to develop a more comprehensive beach analysis strategy that not only addresses presently uncharacterized shores and sediments but also recognizes the importance of eroded flocs as a vector for the transport of bacteria in aquatic environments.

2021 ◽  
Author(s):  
Andrew Sousa

The sampling regime used to monitor the microbiological quality of water typically involves the collection of whole water samples, where bacteria are assumed to be planktonic. This practice ignores sedimentary pathogen sources and highlights the lack of understanding regarding the effect of shear stress on the erosion of bacteria from sediment particles. This study utilized a wave flume and an environmental test bacterial strain to examine the effect of increasing wave energy on bacterial loading and the partitioning of free-floating and floc-associated bacteria in water. A positive correlation was found between wave energy, total suspended solids, and bacterial loading in water. Experiments examining free-floating and floc-associated bacteria under low (0.60 N/s) and high (5.35 N/s) wave energy demonstrated the importance of floc as a vector for the transport of bacteria. These results imply that current beach sampling and analysis methods may not reflect overall beach water quality.


2021 ◽  
Author(s):  
Andrew Sousa

The sampling regime used to monitor the microbiological quality of water typically involves the collection of whole water samples, where bacteria are assumed to be planktonic. This practice ignores sedimentary pathogen sources and highlights the lack of understanding regarding the effect of shear stress on the erosion of bacteria from sediment particles. This study utilized a wave flume and an environmental test bacterial strain to examine the effect of increasing wave energy on bacterial loading and the partitioning of free-floating and floc-associated bacteria in water. A positive correlation was found between wave energy, total suspended solids, and bacterial loading in water. Experiments examining free-floating and floc-associated bacteria under low (0.60 N/s) and high (5.35 N/s) wave energy demonstrated the importance of floc as a vector for the transport of bacteria. These results imply that current beach sampling and analysis methods may not reflect overall beach water quality.


Water ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 992 ◽  
Author(s):  
Dogan Kisacik ◽  
Vasiliki Stratigaki ◽  
Minghao Wu ◽  
Lorenzo Cappietti ◽  
Irene Simonetti ◽  
...  

Floating oscillating water column (OWC) type wave energy converters (WECs), compared to fixed OWC WECs that are installed near the coastline, can be more effective as they are subject to offshore waves before the occurrence of wave dissipation at a nearshore location. The performance of floating OWC WECs has been widely studied using both numerical and experimental methods. However, due to the complexity of fluid–structure interaction of floating OWC WECs, most of the available studies focus on 2D problems with WEC models of limited degrees-of-freedom (DOF) of motion, while 3D mooring effects and multiple-DOF OWC WECs have not been extensively investigated yet under 2D and 3D wave conditions. Therefore, in order to gain a deeper insight into these problems, the present study focuses on wave flume experiments to investigate the motion and mooring performance of a scaled floating OWC WEC model under 2D wave conditions. As a preparatory phase for the present MaRINET2 EsflOWC (efficiency and survivability of floating OWC) project completed at the end of 2017, experiments were also carried out in advance in the large wave flume of Ghent University. The following data were obtained during these experimental campaigns: multiple-DOF OWC WEC motions, mooring line tensions, free surface elevations throughout the wave flume, close to and inside the OWC WEC, change in the air pressure inside the OWC WEC chamber and velocity of the airflow through the vent on top of the model. The tested wave conditions mostly include nonlinear intermediate regular waves. The data obtained at the wave flume of Ghent University, together with the data from the EsflOWC tests at the wave flume of LABIMA, University of Florence, provide a database for numerical validation of research on floating OWC WECs and floating OWC WEC farms or arrays used by researchers worldwide.


2009 ◽  
Vol 8 (1) ◽  
pp. 11-19 ◽  
Author(s):  
Marta Sofia Valente ◽  
Paulo Pedro ◽  
M. Carmen Alonso ◽  
Juan J. Borrego ◽  
Lídia Dionísio

Monitoring the microbiological quality of water used for recreational activities is very important to human public health. Although the sanitary quality of recreational marine waters could be evaluated by standard methods, they are time-consuming and need confirmation. For these reasons, faster and more sensitive methods, such as the defined substrate-based technology, have been developed. In the present work, we have compared the standard method of membrane filtration using Tergitol-TTC agar for total coliforms and Escherichia coli, and Slanetz and Bartley agar for enterococci, and the IDEXX defined substrate technology for these faecal pollution indicators to determine the microbiological quality of natural recreational waters. ISO 17994:2004 standard was used to compare these methods. The IDEXX for total coliforms and E. coli, Colilert®, showed higher values than those obtained by the standard method. Enterolert® test, for the enumeration of enterococci, showed lower values when compared with the standard method. It may be concluded that more studies to evaluate the precision and accuracy of the rapid tests are required in order to apply them for routine monitoring of marine and freshwater recreational bathing areas. The main advantages of these methods are that they are more specific, feasible and simpler than the standard methodology.


2014 ◽  
Vol 64 ◽  
pp. 255-265 ◽  
Author(s):  
Yongyao Luo ◽  
Jean-Roch Nader ◽  
Paul Cooper ◽  
Song-Ping Zhu

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