Population Genetic Structure of the Armorhead, Pseudopentaceros wheeleri, in the North Pacific Ocean: Application of the Polymerase Chain Reaction to Fisheries problems

1992 ◽  
Vol 49 (11) ◽  
pp. 2386-2391 ◽  
Author(s):  
Andrew P. Martin ◽  
Robert Humphreys ◽  
Stephen R. Palumbi
Keyword(s):  
Polymerase Chain Reaction ◽  
Pacific Ocean ◽  
North Pacific Ocean ◽  
Rflp Analysis ◽  
Open Ocean ◽  
Chain Reaction ◽  
Central Pacific ◽  
The North ◽  
Northern Pacific ◽  
Polymerase Chain

Armorhead (Pseudopentaceros wheeleri) occur in the subarctic, epipelagic habitats of the northern Pacific Ocean and are known to reproduce on seamounts in the central Pacific. Over the last few decades, overexploitation of seamount populations led to dramatic declines in abundances of reproductive populations. We undertook a study of the population genetics of armorhead to test whether distinct stocks exist in association with specific seamounts. We used the polymerase chain reaction (PCR) and a combination of DNA sequencing and restriction fragment length polymorphism (RFLP) analysis to analyze mtDNA variants for individuals collected from three localities: two seamounts and from the open ocean. We discovered that mtDNA haplotypes are not partitioned geographically, refuting the hypothesis that different seamounts harbor genetically distinct populations. Furthermore, genetic similarity of seamount and open-ocean fish supports the hypothesis that armorhead migrate between the central and northern Pacific Ocean for reproduction and feeding, respectively.

Population genetics, phylogeography, and systematics of the thornyhead rockfishes (Sebastolobus) along the deep continental slopes of the North Pacific Ocean

2000 ◽  
Vol 57 (8) ◽  
pp. 1701-1717 ◽  
Author(s):  
Carol A Stepien ◽  
Alison K Dillon ◽  
Amy K Patterson
Keyword(s):  
Pacific Ocean ◽  
Phylogenetic Trees ◽  
Sequence Data ◽  
North Pacific Ocean ◽  
Geographic Distance ◽  
The North ◽  
Continental Slopes ◽  
Genetic Patterns ◽  
Northern Pacific ◽  
Shortspine Thornyhead

Population genetic, phylogeographic, and systematic relationships are elucidated among the three species comprising the thornyhead rockfish genus Sebastolobus (Teleostei: Scorpaenidae). Genetic variation among sampling sites representing their extensive ranges along the deep continental slopes of the northern Pacific Ocean is compared using sequence data from the left domain of the mtDNA control region. Comparisons are made among the shortspine thornyhead (S. alascanus) (from seven locations), the longspine thornyhead (S. altivelis) (from five sites), which are sympatric in the northeast, and the broadbanded thornyhead (S. macrochir) (a single site) from the northwest. Phylogenetic trees rooted to Sebastes show that S. macrochir is the sister taxon of S. alascanus and S. altivelis. Intraspecific genetic variability is appreciable, with most individuals having unique haplotypes. Gene flow is substantial among some locations and others diverged significantly. Genetic divergences among sampling sites for S. alascanus indicate an isolation by geographic distance pattern. Genetic divergences for S. altivelis are unrelated to the hypothesis of isolation by geographic distance and appear to be more consistent with the hypothesis of larval retention in currents and gyres. Differences in geographic genetic patterns between the species are attributed to life history differences in their relative mobilities as juveniles and adults.

Central Pacific El Niño and decadal climate change in the North Pacific Ocean

2010 ◽  
Vol 3 (11) ◽  
pp. 762-765 ◽  
Author(s):  
E. Di Lorenzo ◽  
K. M. Cobb ◽  
J. C. Furtado ◽  
N. Schneider ◽  
B. T. Anderson ◽  
...  
Keyword(s):  
Climate Change ◽  
Pacific Ocean ◽  
North Pacific ◽  
El Nino ◽  
North Pacific Ocean ◽  
Central Pacific ◽  
Central Pacific El Niño ◽  
The North ◽  
Decadal Climate ◽  
The North Pacific

Genetic analysis of nuclear DNA restriction fragment patterns

Genome ◽  
1991 ◽  
Vol 34 (5) ◽  
pp. 693-703 ◽  
Author(s):  
Elizabeth M. Gillet
Keyword(s):  
Polymerase Chain Reaction ◽  
Restriction Fragment Length Polymorphism ◽  
Genetic Analysis ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length Polymorphism ◽  
Rflp Analysis ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Restriction Fragment Length

Restriction fragment length polymorphism (RFLP) analysis in the broad sense is the analysis of differences in restriction fragment pattern produced by defined target segments within or between cell compartments, cell types, etc., in a single individual or in different individuals. Thus both molecular hybridization and DNA amplification by two-primer extension using the polymerase chain reaction can define target segments for RFLP analysis. The two techniques are outlined with special consideration of characteristics important for genetic analysis. The mode of inheritance of restriction fragment patterns as a prerequisite for their use as genetic markers in inheritance studies is explained, leading to criticism of common usage. The importance of internal restriction sites for the determination of allelic variation is stressed. It is shown that, if target segments are under the control of a single nuclear diploid restriction fragment locus, then complete reconstruction of all parental target segments requires controlled crosses between individuals of like restriction fragment pattern.Key words: genetic analysis, inheritance, restriction fragment length polymorphism, controlled cross, polymerase chain reaction.

Relationships amongFusariumspp. estimated by comparing restriction fragment length polymorphisms in polymerase chain reaction-amplified nuclear rDNA

1996 ◽  
Vol 42 (12) ◽  
pp. 1232-1240 ◽  
Author(s):  
G. L. Bateman ◽  
E. Ward ◽  
H. Kwaśna
Keyword(s):  
Polymerase Chain Reaction ◽  
Restriction Fragment ◽  
Fragment Length ◽  
Polymerase Chain Reaction Amplification ◽  
Morphological Characteristics ◽  
Rflp Analysis ◽  
Nuclear Rdna ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Restriction Fragment Length

Nuclear rDNA from 120 isolates of 34 Fusarium spp. and Microdochium nivale was compared by restriction fragment length polymorphism (RFLP) analysis after polymerase chain reaction amplification. The RFLPs allowed differentiation between species or groups of species. The presence or absence of each of 75 DNA bands was also used to compile a similarity matrix for cluster analysis to show estimated phylogenetic relationships. There was mostly little diversity between isolates of the same species. However, there were at least two distinct genetic types among isolates that conformed morphologically to each of the species F. avenaceum, F. sambucinum, F. flocciferum, and F. proliferatum. Most relationships were consistent with current understanding of Fusarium taxonomy. The division into taxonomic sections based on morphological characteristics was generally not supported.Key words: Fusarium, rDNA, phylogeny.

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