A peach linkage map integrating RFLPs, SSRs, RAPDs, and morphological markers

Genome ◽  
2001 ◽  
Vol 44 (5) ◽  
pp. 783-790 ◽  
Author(s):  
Maria Teresa Dettori ◽  
Roberta Quarta ◽  
Ignazio Verde
Keyword(s):  
Molecular Markers ◽  
Comparative Analysis ◽  
Linkage Map ◽  
Morphological Traits ◽  
Prunus Persica ◽  
Average Distance ◽  
Morphological Markers ◽  
Linkage Groups ◽  
Peach Genome ◽  
The Common

A linkage map was obtained using a BC1 progeny (Prunus persica × (P. persica × P. ferganensis)). The map is composed of 109 loci (74 RFLPs, 17 SSRs, 16 RAPDs, and two morphological traits) distributed in 10 linkage groups. Loci, segregating in five different ratios, were integrated in the map with JoinMap 2.0 software. The map covers 521 cM of the peach genome. The average distance between adjacent loci is 4.8 cM. Two monogenic traits, flesh adhesion (F/f) and leaf glands (E/e), were placed on the map. Thirty-two loci in common with a saturated linkage map of Prunus allowed a comparative analysis to be made between the two maps. Homologies were found among the respective linkage groups. No relevant differences were observed in the linear order of the common loci.Key words: peach, linkage map, Prunus persica, Prunus ferganensis, molecular markers.

scholarly journals Generation of a Highly Saturated Linkage Map in Peach [Prunus persica (L.) Batsch] Using AFLP and RFLP Markers

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 592b-592
Author(s):  
Bryon Sosinski ◽  
W.V. Baird ◽  
S. Rajapakse ◽  
R.E. Ballard ◽  
A.G. Abbott
Keyword(s):  
Linkage Map ◽  
Genetic Linkage Map ◽  
Prunus Persica ◽  
Quality Characteristics ◽  
Soluble Solids ◽  
Rflp Markers ◽  
Linkage Groups ◽  
Flesh Color ◽  
Peach Genome ◽  
The Cross

We have developed a highly saturated genetic linkage map in peach (diploid, 2n = 16) using two separate crosses. The first population consists of 48 randomly selected F2 individuals which were generated by selfing an F1 from the cross of `New Jersey Pillar' x KV 77119. This progeny set exhibits segregation for gross morphological traits including: canopy shape, fruit flesh color, and flower petal color, size, and number. The second population contains 48 F2 progeny derived from the cross of `Suncrest' x `Bailey'. These progeny segregate for quality traits such as fruit diameter, weight, flesh color, cling vs. free stone, soluble solids, pH of juice extract, and fruit developmental period. Nine linkage groups were identified in the first cross, which cover 590 cM of the genome. In the second cross, eight linkage groups were found that contain several significant chromosomal intervals contributing to fruit quality characteristics by QTL analysis. Anchor loci present in both maps were used to join the linkage groups to create a single combined map of the peach genome. Physical mapping is currently underway to assign the each linkage group to the appropriate chromosome.

Linkage of random amplified polymorphic DNA, isozyme and morphological markers in grasspea (Lathyrus sativus)

1999 ◽  
Vol 133 (4) ◽  
pp. 389-395 ◽  
Author(s):  
M. A. CHOWDHURY ◽  
A. E. SLINKARD
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Rapd Markers ◽  
Genetic Linkage Map ◽  
Average Distance ◽  
Random Amplified Polymorphic Dna ◽  
Lathyrus Sativus ◽  
Morphological Markers ◽  
Linkage Studies ◽  
Linkage Groups

We constructed a genetic linkage map of grasspea (Lathyrus sativus L.; 2n = 14) from 100 F2 individuals derived from a cross between PI 426891.1.3 and PI 283564c.3.2. A total of 71 RAPD, three isozyme and one morphological markers segregated in the F2 progeny. A small fraction of markers (12%) deviated significantly from the expected Mendelian ratio (1[ratio ]2[ratio ]1 or 3[ratio ]1). Out of 75 markers, 69 (one morphological, three isozyme and 65 RAPD markers) were assigned to 14 linkage groups comprising 898 cM. The average distance between two adjacent markers was 17·2 cM. The present linkage map will serve as a reference point for further linkage studies in grasspea.

scholarly journals Location of Four Morphological Markers (dgs, blu, arg, and y) in a Molecular Linkage Map of Common Bean

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 544B-544
Author(s):  
Gino Beltran ◽  
Geunhwa Jung ◽  
Mark Bassett ◽  
James Nienhuis
Keyword(s):  
Molecular Markers ◽  
Common Bean ◽  
Linkage Map ◽  
Morphological Traits ◽  
Rapd Markers ◽  
Genetic Relationships ◽  
Blue Flower ◽  
Recurrent Parent ◽  
Morphological Markers ◽  
Molecular Linkage Map

The development of a complete linkage map including both morphological and molecular markers is important to understand the genetic relationships among quantitatively and qualitatively inherited traits in common bean. The objective of this study was to identify RAPD markers linked to genes for four morphological traits using bulked segregant analysis and to map the markers to a molecular linkage map previously constructed in common bean. Three segregating populations were evaluated. Two BC2F2 populations with dgs (dark green savoy leaf) and blu (blue flower) induced mutant was developed with a Florida breeding line 7-1404 and 5-593 as the recurrent parent. In addition, a BC3F2 population with the y (yellow wax pod) and the arg (silvery green pod) mutants was developed from the Lamprecht line PI 527858 and 5-593 as the recurrent parent. RAPD markers linked in coupling to the morphological traits were detected to be 4.1 cM, 4.3 cM, 7.3 cM, and 12.3 cM distant from the dgs, blu, y, and arg genes, respectively. The linked RAPD markers were mapped in the molecular linkage map previously constructed using recombinant inbred population of the cross PC-50 × XAN-159. In this linkage map, we observed a linkage between the C locus and blu gene whose location was not previously known. In addition, a linkage between an abaxial leaf pubescent gene and dgs gene was observed. These results indicate that integrating morphological markers and molecular markers can result in a more complete genetic linkage map in common bean.

scholarly journals (261) A PCR-based Linkage Map of Broccoli [Brassica oleracea(L.) var. italica] and Comparisons with Existing BrassicaMaps

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1090B-1090
Author(s):  
Allan Brown ◽  
Khalid Ibrahim ◽  
Sultana Islam ◽  
Elizabeth Jeffery ◽  
John Juvik
Keyword(s):  
Molecular Markers ◽  
Genetic Analysis ◽  
Linkage Map ◽  
Brassica Oleracea ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Morphological Markers ◽  
Linkage Groups ◽  
Interval Width ◽  
Brassica Oleracea L

A set of 216 PCR-based molecular markers was screened for polymorphisms using two morphologically dissimilar broccoli lines, `VI-158' and `BNC'. Fifty-nine of these markers, representing 69 detected polymorphisms and two morphological markers, were used to construct a genetic linkage map of broccoli [Brassicaoleracea (L.) var. italica] from a population of 162 F2:3 families generated from the cross between these two lines. Ten genetic linkage groups were generated that spanned a distance of 468 cM with an average interval width of 9.4 cm. This map represents the first combined SSR and SRAP map of Brassica oleracea. Comparisons are made to existing maps of Brassicanapus and to inter-specific maps of Brassicaoleracea. To our knowledge this is the first linkage map of broccoli [Brassicaoleracea (L.) var. italica] and should provide a useful tool for the genetic analysis of traits specific to the italica subspecies.

A genetic linkage map for Stevia rebaudiana

Genome ◽  
1999 ◽  
Vol 42 (4) ◽  
pp. 657-661 ◽  
Author(s):  
Y Yao ◽  
M Ban ◽  
J Brandle
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Rapd Markers ◽  
Genetic Linkage Map ◽  
Average Distance ◽  
Stevia Rebaudiana ◽  
Linkage Groups ◽  
Genome Map ◽  
High Level ◽  
Map Distance

To lay a foundation for molecular breeding efforts, the first genetic linkage map for Stevia rebaudiana has been constructed using segregation data from a pseudo test-cross F1 population. A total of 183 randomly amplified polymorphic DNA (RAPD) markers were analysed and assembled into 21 linkage groups covering a total distance of 1389 cM, with an average distance between markers of of 7.6 cM. The 11 largest linkage groups consisted of 4-19 loci, ranged in length from 56 to 174 cM, and accounted for 75% of the total map distance. Fifteen RAPD loci were found to be unlinked. From the 521 primers showing amplification products, 185 (35.5%) produced a total of 293 polymorphic fragments, indicating a high level of genetic diversity in stevia. Most of the RAPD markers in stevia segregated in normal Mendelian fashion.Key words: stevia, open-pollinated, genome map, RAPD.

Integration of molecular and classical linkage groups of the silkworm, Bombyx mori (n = 28)

Genome ◽  
2005 ◽  
Vol 48 (4) ◽  
pp. 626-629 ◽  
Author(s):  
Yuji Yasukochi ◽  
Yutaka Banno ◽  
Kohji Yamamoto ◽  
Marian R Goldsmith ◽  
Hiroshi Fujii
Keyword(s):  
Molecular Markers ◽  
Linkage Group ◽  
Linkage Analysis ◽  
Linkage Map ◽  
Bombyx Mori ◽  
Linkage Maps ◽  
Wild Type ◽  
Linkage Groups ◽  
Sequence Tagged Sites ◽  
Silkworm Bombyx Mori

Previously published linkage groups (LGs) composed of molecular markers were assigned to classical LGs in the silkworm, Bombyx mori (n = 28). Four markers from the classical linkage map, og, w-1, Lp, and Pfl, were assigned to the molecular linkage maps using sequence tagged sites. In addition, linkage analysis was carried out using BF1 progeny between wild-type and mutant stocks carrying morphological phenotypic markers. As a result, the counterparts for 26 of 28 molecular LGs were identified with their counterparts of the classical LGs. Two visible markers, Sel and Xan, representing different classical LGs, were found to be linked.Key words: Bombyx mori, classical linkage group (LG), PCR-based genotyping, mutant, STS.

Construction of a reference linkage map for melon

Genome ◽  
2001 ◽  
Vol 44 (5) ◽  
pp. 836-845 ◽  
Author(s):  
M Oliver ◽  
J Garcia-Mas ◽  
M Cardús ◽  
N Pueyo ◽  
A I López-Sesé ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Genetic Distance ◽  
Linkage Map ◽  
Genetic Map ◽  
Cucumis Melo ◽  
Morphological Trait ◽  
Linkage Groups ◽  
Resistant Gene ◽  
Anchor Points ◽  
Cucumis Melo L

A map of melon (Cucumis melo L.) with 411 markers (234 RFLPs, 94 AFLPs, 47 RAPDs, 29 SSRs, five inter-SSRs, and two isozymes) and one morphological trait (carpel number) was constructed using the F2 progeny of a cross between the Korean accession PI161375 and the Spanish melon type 'Pinyonet Piel de Sapo'. RFLPs were obtained using 212 probes from different genomic and cDNA melon libraries, including 16 Arabidopsis ESTs, 13 Cucumis known genes, and three resistant gene homologues. Most loci (391) mapped to 12 major linkage groups, spanning a total genetic distance of 1197 cM, with an average map interval of 3 cM/marker. The remaining 21 loci (six RAPDs and 15 AFLPs) were not linked. A majority (66%) of the markers were codominant (RFLPs, SSRs, and isozymes), making them easily transferable to other melon crosses. Such markers can be used as a reference, to merge other melon and cucumber maps already constructed. Indeed, some of them (23 SSRs, 14 RFLPs, one isozyme, and one morphological trait) could act as anchor points with other published cucurbit maps.Key words: Cucumis melo, genetic map, molecular markers, RFLPs, SSRs.

Genetic linkage map of peach [Prunus persica (L.) Batsch] using morphological and molecular markers

1998 ◽  
Vol 97 (5-6) ◽  
pp. 888-895 ◽  
Author(s):  
E. Dirlewanger ◽  
V. Pronier ◽  
C. Parvery ◽  
C. Rothan ◽  
A. Guye ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Prunus Persica

Construction of a genetic linkage map of black gram, Vigna mungo (L.) Hepper, based on molecular markers and comparative studies

Genome ◽  
2008 ◽  
Vol 51 (8) ◽  
pp. 628-637 ◽  
Author(s):  
S. K. Gupta ◽  
J. Souframanien ◽  
T. Gopalakrishna
Keyword(s):  
Molecular Markers ◽  
Linkage Group ◽  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Average Distance ◽  
Vigna Mungo ◽  
Black Gram ◽  
Recombinant Inbred Population ◽  
Average Marker Density

A genetic linkage map of black gram, Vigna mungo (L.) Hepper, was constructed with 428 molecular markers using an F9 recombinant inbred population of 104 individuals. The population was derived from an inter-subspecific cross between a black gram cultivar, TU94-2, and a wild genotype, V. mungo var. silvestris. The linkage analysis at a LOD score of 5.0 distributed all 428 markers (254 AFLP, 47 SSR, 86 RAPD, and 41 ISSR) into 11 linkage groups. The map spanned a total distance of 865.1 cM with an average marker density of 2 cM. The largest linkage group spanned 115 cM and the smallest linkage group was of 44.9 cM. The number of markers per linkage group ranged from 11 to 86 and the average distance between markers varied from 1.1 to 5.6 cM. Comparison of the map with other published azuki bean and black gram maps showed high colinearity of markers, with some inversions. The current map is the most saturated map for black gram to date and will provide a useful tool for identification of QTLs and for marker-assisted selection of agronomically important characters in black gram.

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