scholarly journals Location of Four Morphological Markers (dgs, blu, arg, and y) in a Molecular Linkage Map of Common Bean

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 544B-544
Author(s):  
Gino Beltran ◽  
Geunhwa Jung ◽  
Mark Bassett ◽  
James Nienhuis
Keyword(s):  
Molecular Markers ◽  
Common Bean ◽  
Linkage Map ◽  
Morphological Traits ◽  
Rapd Markers ◽  
Genetic Relationships ◽  
Blue Flower ◽  
Recurrent Parent ◽  
Morphological Markers ◽  
Molecular Linkage Map

The development of a complete linkage map including both morphological and molecular markers is important to understand the genetic relationships among quantitatively and qualitatively inherited traits in common bean. The objective of this study was to identify RAPD markers linked to genes for four morphological traits using bulked segregant analysis and to map the markers to a molecular linkage map previously constructed in common bean. Three segregating populations were evaluated. Two BC2F2 populations with dgs (dark green savoy leaf) and blu (blue flower) induced mutant was developed with a Florida breeding line 7-1404 and 5-593 as the recurrent parent. In addition, a BC3F2 population with the y (yellow wax pod) and the arg (silvery green pod) mutants was developed from the Lamprecht line PI 527858 and 5-593 as the recurrent parent. RAPD markers linked in coupling to the morphological traits were detected to be 4.1 cM, 4.3 cM, 7.3 cM, and 12.3 cM distant from the dgs, blu, y, and arg genes, respectively. The linked RAPD markers were mapped in the molecular linkage map previously constructed using recombinant inbred population of the cross PC-50 × XAN-159. In this linkage map, we observed a linkage between the C locus and blu gene whose location was not previously known. In addition, a linkage between an abaxial leaf pubescent gene and dgs gene was observed. These results indicate that integrating morphological markers and molecular markers can result in a more complete genetic linkage map in common bean.

Mapping of Five Morphological Markers (dgs, blu, rnd, Ib, and asp) in a Molecular Linkage Map of Common Bean

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 547c-547
Author(s):  
Gino Beltran ◽  
Geunhwa Jung ◽  
Mark Bassett ◽  
James Nienhuis
Keyword(s):  
Common Bean ◽  
Linkage Map ◽  
Genetic Linkage ◽  
Rapd Markers ◽  
Genetic Linkage Map ◽  
Bulked Segregant Analysis ◽  
Blue Flower ◽  
Recurrent Parent ◽  
Molecular Linkage Map ◽  
Dark Green

The classical genetic linkage map of common bean contains only a fraction of the total genes that has been reported. These genes can be mapped in a molecular linkage map so that genetic relationship among diferent genes can be better understood. The objective of this study was to identify RAPD markers linked to genes for five morphological traits using bulked segregant analysis and to map the markers to a molecular linkage map previously constructed in common bean. Five segregating populations were evaluated. Three BC2F2 populations with dgs (dark green savoy leaf), blu (blue flower), and rnd (round leaf), respectively, were developed with a Florida breeding line 7-1404 and 5-593 as the recurrent parent. One BC3F2 population with the asp (dull seed coat) was developed from a BC2F2 5-593 line and 5-593 as the recurrent parent. Finally, an F2 segregating population for Ib (flat pod) was developed from `Hialeah' flat pod mutant × `Hialeah'. The linked RAPD markers were mapped in a molecular linkage map previously constructed using recombinant inbred population of the cross PC-50 × XAN-159. The results of this study indicate that integrating morphological and molecular merkers can result in a more complete genetic linkage map in common bean.

scholarly journals Identification of RAPD Markers Linked to Five Marker Genes (blu, dgs, y, arg, and a Flat Pod Mutant) in Common Bean

2002 ◽  
Vol 127 (4) ◽  
pp. 685-688 ◽  
Author(s):  
Gino E. Beltrán ◽  
Geunwha Jung ◽  
James Nienhuis ◽  
Mark J. Bassett
Keyword(s):  
Molecular Markers ◽  
Common Bean ◽  
Linkage Map ◽  
Rapd Markers ◽  
Genetic Relationships ◽  
Random Amplified Polymorphic Dna ◽  
Spontaneous Mutation ◽  
Marker Genes ◽  
Dark Green ◽  
Mapping Populations

The development of a complete linkage map, including both classical (visible) and molecular markers, is important to understand the genetic relationships among different traits in common bean (Phaseolus vulgaris L.). The objective of this study was to integrate classical marker genes into previously constructed molecular linkage maps in common bean. Bulked segregant analysis was used to identify 10 random amplified polymorphic DNA (RAPD) markers linked to genes for five classical marker traits: dark green savoy leaf (dgs), blue flower (blu), silvery [Latin: argentum] green pod (arg), yellow wax pod (y) and flat pod (a spontaneous mutation from round to flat pod in `Hialeah' snap bean). The genes for dark green savoy leaf (dgs) and blue flower (blu) were located in a previously constructed molecular linkage map. These results indicate that classical marker genes and molecular markers can be integrated to form a more complete and informative genetic linkage map. Most of the RAPD markers were not polymorphic in the two mapping populations used, and molecular markers from those mapping populations were not polymorphic in the F2 populations used to develop the RAPD markers. Alternative genetic hypotheses for the pod shape mutation in `Hialeah' are discussed, and the experimental difficulties of pod shape classification are described.

A peach linkage map integrating RFLPs, SSRs, RAPDs, and morphological markers

Genome ◽  
2001 ◽  
Vol 44 (5) ◽  
pp. 783-790 ◽  
Author(s):  
Maria Teresa Dettori ◽  
Roberta Quarta ◽  
Ignazio Verde
Keyword(s):  
Molecular Markers ◽  
Comparative Analysis ◽  
Linkage Map ◽  
Morphological Traits ◽  
Prunus Persica ◽  
Average Distance ◽  
Morphological Markers ◽  
Linkage Groups ◽  
Peach Genome ◽  
The Common

A linkage map was obtained using a BC1 progeny (Prunus persica × (P. persica × P. ferganensis)). The map is composed of 109 loci (74 RFLPs, 17 SSRs, 16 RAPDs, and two morphological traits) distributed in 10 linkage groups. Loci, segregating in five different ratios, were integrated in the map with JoinMap 2.0 software. The map covers 521 cM of the peach genome. The average distance between adjacent loci is 4.8 cM. Two monogenic traits, flesh adhesion (F/f) and leaf glands (E/e), were placed on the map. Thirty-two loci in common with a saturated linkage map of Prunus allowed a comparative analysis to be made between the two maps. Homologies were found among the respective linkage groups. No relevant differences were observed in the linear order of the common loci.Key words: peach, linkage map, Prunus persica, Prunus ferganensis, molecular markers.

scholarly journals Integration of the classical and molecular linkage maps of tomato chromosome 6.

Genetics ◽  
1993 ◽  
Vol 135 (4) ◽  
pp. 1175-1186
Author(s):  
R Weide ◽  
M F van Wordragen ◽  
R K Lankhorst ◽  
R Verkerk ◽  
C Hanhart ◽  
...  
Keyword(s):  
Linkage Map ◽  
Rapd Markers ◽  
Linkage Data ◽  
Chromosome 6 ◽  
Morphological Markers ◽  
Limited Information ◽  
Tomato Genome ◽  
The Past ◽  
Molecular Linkage Map ◽  
Tomato Chromosome

Abstract In the past, a classical map of the tomato genome has been established that is based on linkage data from intraspecific Lycopersicon esculentum crosses. In addition, a high density molecular linkage map has recently been constructed using a L. esculentum x L. pennellii cross. As the respective maps only partially match, they provide limited information about the relative positions of classical and molecular markers. In this paper we describe the construction of an integrated linkage map of tomato chromosome 6 that shows the position of cDNA-, genomic DNA- and RAPD markers relative to 10 classical markers. Integration was achieved by using a L. esculentum line containing an introgressed chromosome 6 from L. pennellii in crosses to a variety of L. esculentum marker lines. In addition, an improved version of the classical linkage map is presented that is based on a combined analysis of new linkage data for 16 morphological markers and literature data. Unlike the classical map currently in use, the revised map reveals clustering of markers into three major groups around the yv, m-2 and c loci, respectively. Although crossing-over rates are clearly different when comparing intraspecific L. esculentum crosses with L. esculentum x L. pennellii crosses, the clusters of morphological markers on the classical map coincide with clusters of genomic- and cDNA-markers on the molecular map constructed by Tanksley and coworkers.

Linkage mapping in diploid alfalfa (Medicago sativa)

Genome ◽  
1994 ◽  
Vol 37 (1) ◽  
pp. 61-71 ◽  
Author(s):  
C. S. Echt ◽  
K. K. Kidwell ◽  
S. J. Knapp ◽  
T. C. Osborn ◽  
T. J. McCoy
Keyword(s):  
Linkage Map ◽  
Linkage Mapping ◽  
Rapd Markers ◽  
Genome Mapping ◽  
Recurrent Parent ◽  
Backcross Population ◽  
Length Polymorphisms ◽  
Combined Use ◽  
A Genome ◽  
Genome Map

A genome map of cultivated alfalfa was constructed using segregating restriction fragment length polymorphisms (RFLPs) and random amplified polymorphic DNAs (RAPDs) in a diploid backcross population generated from noninbred parents. Among the 153 loci scored in 87 progeny, four segregation ratios were observed for codominant and dominant markers: 1:1, 1:2:1, 1:1:1:1, and 3:1. Deviations from expected Mendelian ratios (p < 0.05) were observed for 34% of the loci studied. A genome map was assembled from two separate linkage maps, each constructed from a subset of the segregation data. One linkage map was constructed from 46 RFLP and 40 RAPD markers segregating 1:1 from the F1 parent of the backcross and the other linkage map was constructed from 33 RFLP and 28 RAPD markers segregating 1:1 from the recurrent parent. Sixteen loci with alleles segregating 1:1 from both parents were used as locus bridges to align individual linkage groups between the two maps. The combined use of RFLPs and RAPDs was an effective method for developing an alfalfa genome map.Key words: genome mapping, RAPD, RFLP, locus bridges.

CONSTRUCTION OF A HIGH DENSITY MOLECULAR LINKAGE MAP FOR LYCHEE BASED ON AFLP AND RAPD MARKERS

2010 ◽  
pp. 87-94 ◽  
Author(s):  
C.M. Liu ◽  
Y.S. Guo ◽  
R. Liu ◽  
B. Zhang ◽  
J.H. Xie ◽  
...  
Keyword(s):  
Linkage Map ◽  
Rapd Markers ◽  
High Density ◽  
Molecular Linkage Map

Linkage of random amplified polymorphic DNA, isozyme and morphological markers in grasspea (Lathyrus sativus)

1999 ◽  
Vol 133 (4) ◽  
pp. 389-395 ◽  
Author(s):  
M. A. CHOWDHURY ◽  
A. E. SLINKARD
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Rapd Markers ◽  
Genetic Linkage Map ◽  
Average Distance ◽  
Random Amplified Polymorphic Dna ◽  
Lathyrus Sativus ◽  
Morphological Markers ◽  
Linkage Studies ◽  
Linkage Groups

We constructed a genetic linkage map of grasspea (Lathyrus sativus L.; 2n = 14) from 100 F2 individuals derived from a cross between PI 426891.1.3 and PI 283564c.3.2. A total of 71 RAPD, three isozyme and one morphological markers segregated in the F2 progeny. A small fraction of markers (12%) deviated significantly from the expected Mendelian ratio (1[ratio ]2[ratio ]1 or 3[ratio ]1). Out of 75 markers, 69 (one morphological, three isozyme and 65 RAPD markers) were assigned to 14 linkage groups comprising 898 cM. The average distance between two adjacent markers was 17·2 cM. The present linkage map will serve as a reference point for further linkage studies in grasspea.

Application of the random amplified polymorphic DNA technique for the detection of polymorphism among wild and cultivated tetraploid wheats

Genome ◽  
1993 ◽  
Vol 36 (3) ◽  
pp. 602-609 ◽  
Author(s):  
Chandrashekhar P. Joshi ◽  
Henry T. Nguyen
Keyword(s):  
Molecular Markers ◽  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Triticum Turgidum ◽  
Rapd Analysis ◽  
Genetic Relationships ◽  
Random Amplified Polymorphic Dna ◽  
Emmer Wheat ◽  
Tetraploid Wheats

Development of a high-density genetic linkage map of cultivated wheats using conventional molecular markers has lagged behind the other major food crops such as rice and tomato because of the large genome size and limited levels of genetic polymorphisms. Recently, random amplified polymorphic DNAs (RAPDs) have been suggested to provide an alternative to visualize more polymorphism. For the construction of a genetic linkage map in tetraploid wheats, one can use a strategy of intersubspecific crosses between the most dissimilar wild and cultivated tetraploid wheats that are easy to hybridize and result in fertile progeny. An assessment of the level of RAPDs among different accessions and varieties of wild and cultivated tetraploid wheats is required to fulfill this objective. We present here the data obtained using RAPD analysis of 40 primers in 20 accessions of wild tetraploid emmer wheats (Triticum turgidum L. ssp. dicoccoides) and 10 genotypes of cultivated tetraploid durum wheats (Triticum turgidum L. ssp. durum) selected from geographically diverse locations. We have observed a higher level of polymorphism among different accessions of wild emmer wheat from Israel, Turkey, and Jordan than the group of cultivated American, Turkish, and Syrian durum wheats. These data have been used to generate a dendrogram suggesting the genetic relationships among these genotypes, and the most dissimilar genotypes are identified for future mapping and gene tagging work.Key words: durum wheat, emmer wheat, genetic similarity, molecular markers, RAPD analysis.

A genetic linkage map of crested wheatgrass based on AFLP and RAPD markers

Genome ◽  
2012 ◽  
Vol 55 (4) ◽  
pp. 327-335 ◽  
Author(s):  
Xiaoxia Yu ◽  
Xiaolei Li ◽  
Yanhong Ma ◽  
Zhuo Yu ◽  
Zaozhe Li
Keyword(s):  
Molecular Markers ◽  
Linkage Map ◽  
Genetic Linkage ◽  
Rapd Markers ◽  
Genetic Linkage Map ◽  
Mapping Population ◽  
Gene Localization ◽  
Linkage Groups ◽  
Crested Wheatgrass ◽  
Map Distance

Using a population of 105 interspecific F2 hybrids derived from a cross between Agropyron mongolicum Keng and Agropyron cristatum (L.) Gaertn. ‘Fairway’ as a mapping population, a genetic linkage map of crested wheatgrass was constructed based on AFLP and RAPD molecular markers. A total of 175 markers, including 152 AFLP and 23 RAPD markers, were ordered in seven linkage groups. The map distance was 416 cM, with a mean distance of 2.47 cM between markers. The number of markers ranged from 13 to 46 in each linkage group and the length of groups ranged from 18 to 104 cM. The research found that 30 out of 175 molecular markers showed segregation distortion, accounting for 17% of all markers. This is the first genetic linkage map of crested wheatgrass. This map will facilitate gene localization, cloning, and molecular marker-assisted selection in the future.

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