Association mapping of seed oil content in Brassica napus and comparison with quantitative trait loci identified from linkage mappingThis article is one of a selection of papers from the conference “Exploiting Genome-wide Association in Oilseed Brassicas: a model for genetic improvement of major OECD crops for sustainable farming”.

Genome ◽  
2010 ◽  
Vol 53 (11) ◽  
pp. 908-916 ◽  
Author(s):  
Jun Zou ◽  
Congcong Jiang ◽  
Zhengying Cao ◽  
Ruiyuan Li ◽  
Yan Long ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Brassica Napus ◽  
Association Mapping ◽  
Quantitative Trait ◽  
Linkage Mapping ◽  
Oil Content ◽  
Seed Oil ◽  
Type B ◽  
Seed Oil Content ◽  
New Type

Association mapping has been used increasingly in natural populations with rich genetic diversity to detect DNA-based markers that are associated with important agronomic traits. Brassica napus is an important oil crop with limited genetic diversity. “New-type” B. napus that is introgressed with subgenomic components from related species has been developed to broaden the genetic basis of “traditional” B. napus . In this study, new-type B. napus lines and a collection of traditional B. napus varieties from different countries were used as two different populations to evaluate seed oil content and to determine the efficacy of association mapping by comparison with previous study of linkage mapping. Relatively rich genetic diversity, but a higher level of linkage disequilibrium was observed in the new-type B. napus as compared with the traditional B. napus . Similarly, a larger variation in oil content and a greater number of associated markers were detected in the population of new-type B. napus . Meanwhile, more than half of the genetic loci, to which the associated markers corresponded, were located within the quantitative trait loci intervals identified previously in linkage mapping experiments, which demonstrated the power of association mapping in B. napus .

Identification of stable QTLs for seed oil content by combined linkage and association mapping in Brassica napus

Plant Science ◽  
2016 ◽  
Vol 252 ◽  
pp. 388-399 ◽  
Author(s):  
Fengming Sun ◽  
Jing Liu ◽  
Wei Hua ◽  
Xingchao Sun ◽  
Xinfa Wang ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Association Mapping ◽  
Oil Content ◽  
Seed Oil ◽  
Seed Oil Content ◽  
Stable Qtls

scholarly journals Identification of Major Quantitative Trait Loci for Seed Oil Content in Soybeans by Combining Linkage and Genome-Wide Association Mapping

2017 ◽  
Vol 8 ◽  
Author(s):  
Yongce Cao ◽  
Shuguang Li ◽  
Zili Wang ◽  
Fangguo Chang ◽  
Jiejie Kong ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Association Mapping ◽  
Quantitative Trait ◽  
Oil Content ◽  
Seed Oil ◽  
Genome Wide Association ◽  
Seed Oil Content ◽  
Genome Wide ◽  
Trait Loci

Analysis of QTL for seed oil content in Brassica napus by association mapping and QTL mapping

Euphytica ◽  
2016 ◽  
Vol 213 (1) ◽  
Author(s):  
Ying Fu ◽  
Dongqing Zhang ◽  
Madeleine Gleeson ◽  
Yaofeng Zhang ◽  
Baogang Lin ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Qtl Mapping ◽  
Association Mapping ◽  
Oil Content ◽  
Seed Oil ◽  
Seed Oil Content

scholarly journals Unravelling the Complex Interplay of Transcription Factors Orchestrating Seed Oil Content in Brassica napus L.

2021 ◽  
Vol 22 (3) ◽  
pp. 1033
Author(s):  
Abirami Rajavel ◽  
Selina Klees ◽  
Johanna-Sophie Schlüter ◽  
Hendrik Bertram ◽  
Kun Lu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factors ◽  
Brassica Napus ◽  
Oil Content ◽  
Seed Oil ◽  
Seed Oil Content ◽  
Complex Interplay ◽  
Brassica Napus L ◽  
Data Set ◽  
Developmental Switches

Transcription factors (TFs) and their complex interplay are essential for directing specific genetic programs, such as responses to environmental stresses, tissue development, or cell differentiation by regulating gene expression. Knowledge regarding TF–TF cooperations could be promising in gaining insight into the developmental switches between the cultivars of Brassica napus L., namely Zhongshuang11 (ZS11), a double-low accession with high-oil- content, and Zhongyou821 (ZY821), a double-high accession with low-oil-content. In this regard, we analysed a time series RNA-seq data set of seed tissue from both of the cultivars by mainly focusing on the monotonically expressed genes (MEGs). The consideration of the MEGs enables the capturing of multi-stage progression processes that are orchestrated by the cooperative TFs and, thus, facilitates the understanding of the molecular mechanisms determining seed oil content. Our findings show that TF families, such as NAC, MYB, DOF, GATA, and HD-ZIP are highly involved in the seed developmental process. Particularly, their preferential partner choices as well as changes in their gene expression profiles seem to be strongly associated with the differentiation of the oil content between the two cultivars. These findings are essential in enhancing our understanding of the genetic programs in both cultivars and developing novel hypotheses for further experimental studies.

scholarly journals Development and screening of EMS mutants with altered seed oil content or fatty acid composition in Brassica napus

2020 ◽  
Vol 104 (5) ◽  
pp. 1410-1422
Author(s):  
Shan Tang ◽  
Dong‐Xu Liu ◽  
Shaoping Lu ◽  
Liangqian Yu ◽  
Yuqing Li ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Brassica Napus ◽  
Acid Composition ◽  
Oil Content ◽  
Seed Oil ◽  
Seed Oil Content

scholarly journals QTL analysis of an intervarietal set of substitution lines in Brassica napus: (i) Seed oil content and fatty acid composition

Heredity ◽  
2003 ◽  
Vol 90 (1) ◽  
pp. 39-48 ◽  
Author(s):  
M J Burns ◽  
S R Barnes ◽  
J G Bowman ◽  
M H E Clarke ◽  
C P Werner ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Brassica Napus ◽  
Acid Composition ◽  
Qtl Analysis ◽  
Oil Content ◽  
Seed Oil ◽  
Seed Oil Content ◽  
Substitution Lines

scholarly journals A Novel Chimeric Mitochondrial Gene Confers Cytoplasmic Effects on Seed Oil Content in Polyploid Rapeseed (Brassica napus)

2019 ◽  
Vol 12 (4) ◽  
pp. 582-596 ◽  
Author(s):  
Jun Liu ◽  
Wanjun Hao ◽  
Jing Liu ◽  
Shihang Fan ◽  
Wei Zhao ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Oil Content ◽  
Seed Oil ◽  
Mitochondrial Gene ◽  
Seed Oil Content ◽  
Cytoplasmic Effects

scholarly journals Transcriptome profiling analysis reveals the role of silique in controlling seed oil content in Brassica napus

PLoS ONE ◽  
2017 ◽  
Vol 12 (6) ◽  
pp. e0179027 ◽  
Author(s):  
Ke-Lin Huang ◽  
Mei-Li Zhang ◽  
Guang-Jing Ma ◽  
Huan Wu ◽  
Xiao-Ming Wu ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Oil Content ◽  
Seed Oil ◽  
Transcriptome Profiling ◽  
Seed Oil Content ◽  
Profiling Analysis

Identification of quantitative trait loci associated with oil content and development of near isogenic lines for stable qOC-A10 in Brasscia napus L.

2016 ◽  
Vol 96 (3) ◽  
pp. 423-432 ◽  
Author(s):  
Xiaomao Cheng ◽  
Shu Xia ◽  
Xihua Zeng ◽  
Jianxun Gu ◽  
Yuan Yang ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Quantitative Trait ◽  
Oil Content ◽  
Seed Oil ◽  
Seed Quality ◽  
Quality Trait ◽  
Near Isogenic Lines ◽  
Seed Oil Content ◽  
Isogenic Lines ◽  
Trait Loci

Seed oil content is a key seed quality trait determining the economic value of rapeseed (Brassica napus L.). However, it is a complex quantitative trait controlled by multiple genes. To this point, its genetic mechanism in rapeseed remains to be revealed. In the present study, we separately identified the quantitative trait loci (QTL) controlling seed oil content of B. napus using three generations of recombinant inbred line (RIL) populations (F4:5, F5:6, and F6:7) derived from a cross of two contrasting parents (M201, a high-oil parent, and M202, a low-oil parent) in four trials. The results indicated that the additive effects may be the primary factors contributing to the variation in seed oil content in B. napus. A total of 15 QTL for seed oil content were mapped. Two of them, namely qOC-A9-3 and qOC-A10, were consistently detected across two and all four environments, respectively. Meanwhile, qOC-A10 showed a large effect on phenotypic variation in seed oil content. The stability and significance of qOC-A10 was also validated in the near isogenic lines (NILs-qOC-A10) developed from the RIL population (F4:5) using marker-assisted selection. The qOC-A10 is of particular interest for further fine mapping and map-based cloning.

scholarly journals Comparative Transcriptome Analysis of Developing Seeds and Silique Wall Reveals Dynamic Transcription Networks for Effective Oil Production in Brassica napus L.

2019 ◽  
Vol 20 (8) ◽  
pp. 1982 ◽  
Author(s):  
Muhammad Shahid ◽  
Guangqin Cai ◽  
Feng Zu ◽  
Qing Zhao ◽  
Muhammad Uzair Qasim ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Seed Development ◽  
Vegetable Oil ◽  
Oil Content ◽  
Seed Oil ◽  
Seed Oil Content ◽  
Oil Accumulation ◽  
Developing Seeds ◽  
Oil Biosynthesis ◽  
Oil Crops

Vegetable oil is an essential constituent of the human diet and renewable raw material for industrial applications. Enhancing oil production by increasing seed oil content in oil crops is the most viable, environmentally friendly, and sustainable approach to meet the continuous demand for the supply of vegetable oil globally. An in-depth understanding of the gene networks involved in oil biosynthesis during seed development is a prerequisite for breeding high-oil-content varieties. Rapeseed (Brassica napus) is one of the most important oil crops cultivated on multiple continents, contributing more than 15% of the world’s edible oil supply. To understand the phasic nature of oil biosynthesis and the dynamic regulation of key pathways for effective oil accumulation in B. napus, comparative transcriptomic profiling was performed with developing seeds and silique wall (SW) tissues of two contrasting inbred lines with ~13% difference in seed oil content. Differentially expressed genes (DEGs) between high- and low-oil content lines were identified across six key developmental stages, and gene enrichment analysis revealed that genes related to photosynthesis, metabolism, carbohydrates, lipids, phytohormones, transporters, and triacylglycerol and fatty acid synthesis tended to be upregulated in the high-oil-content line. Differentially regulated DEG patterns were revealed for the control of metabolite and photosynthate production in SW and oil biosynthesis and accumulation in seeds. Quantitative assays of carbohydrates and hormones during seed development together with gene expression profiling of relevant pathways revealed their fundamental effects on effective oil accumulation. Our results thus provide insights into the molecular basis of high seed oil content (SOC) and a new direction for developing high-SOC rapeseed and other oil crops.

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