CHROMOSOME ABERRATIONS IN TRYPAN BLUE INDUCED TERATOGENESIS IN MICE

1968 ◽  
Vol 10 (1) ◽  
pp. 91-98 ◽  
Author(s):  
M. Joneja ◽  
S. Ungthavorn
Keyword(s):  
Chromosome Aberrations ◽  
Trypan Blue ◽  
Chromosomal Rearrangements ◽  
Embryonic Cells ◽  
Teratogenic Effects ◽  
Factors Affecting ◽  
High Incidence ◽  
Direct Causal Relationship ◽  
Abnormal Cells ◽  
Chromosome Bridges

Pregnant Swiss mice were injected with trypan blue on the 9th day of gestation. Analyses of anaphase-telophases revealed a high incidence of mitotic irregularities such as chromosome bridges, fragments or both, in the embryos. As a result of chromosomal rearrangements followed by mitotic aberrations, karyotypically abnormal cells were formed in the embryos and they had a relatively higher incidence of such abnormal cells than the untreated controls. This study does not suggest any direct causal relationship between chromosomal lesions induced and the teratogenic effects of trypan blue. However, it is possible that these aberrations are factors affecting the viability and proliferative capacities of the embryonic cells.

scholarly journals Management of Giant Gastric Ulcer Perforation: Report of a Case and Review of the Literature

2016 ◽  
Vol 2016 ◽  
pp. 1-3
Author(s):  
Nitin Vashistha ◽  
Dinesh Singhal ◽  
Gurpreet Makkar ◽  
Suneel Chakravarty ◽  
Vivek Raj
Keyword(s):  
Gastric Ulcer ◽  
Multidetector Ct ◽  
Symptom Relief ◽  
Gastric Ulcers ◽  
Review Of The Literature ◽  
H2 Receptor Antagonist ◽  
Factors Affecting ◽  
High Incidence ◽  
Surgical Options

Giant gastric ulcer (GGU) is defined as an ulcer more than 3 cm in diameter. Now infrequent in clinical practice, in the pre-H2 receptor antagonist (H2RA) era, the incidence of GGU varied between 12 and 24% of all gastric ulcers. Proton pump inhibitors reportedly achieve better healing rates and symptom relief in comparison to H2RA. The GGU is associated with high incidence of serious complications such as hemorrhage. A perforated GGU though rare (<2%) offers serious challenges in management. We report one such case wherein the role of multidetector CT scan (MDCT) for diagnosis and treatment planning, surgical options for GGU perforations, and factors affecting outcome are discussed.

Teratogenic Effects of Trypan Blue on Amphibian Embryos

Development ◽  
1956 ◽  
Vol 4 (2) ◽  
pp. 110-118
Author(s):  
C. H. Waddington ◽  
M. M. Perry
Keyword(s):  
Trypan Blue ◽  
Teratogenic Effects ◽  
Pregnant Rat ◽  
Vital Dye ◽  
Amphibian Embryos ◽  
Mammalian Embryos

Considerable interest has been aroused by the report of Gillman, Gilbert, Gillman, & Spence (1948) that the vital dye trypan blue when injected into the pregnant rat brings about the appearance of various abnormalities in the offspring. The mammal embryo is notoriously difficult to attack experimentally, and trypan blue therefore seems to provide a valuable tool which, moreover, might throw light on the mechanisms of the important effects which are known in some cases to be produced on the foetus by pathological states in the mother. Further studies on the effects of injected trypan blue on mammalian embryos have therefore been made by Hamburgh (1952, 1954), Waddington & Carter (1952, 1953), and Murakami and his collaborators (1952, 1954), all of whom worked on the mouse, and by Harm (1954) who studied the rabbit. All these authors agree, on the whole, in the type of results which they have obtained, although there are some minor differences between their reports which will be considered later.

Factors affecting the sensitivity of rabbit lymphoid and bone marrow cells to Streptonigrin-induced chromosome aberrations

1981 ◽  
Vol 30 (1) ◽  
pp. 15-24 ◽  
Author(s):  
V. Huff ◽  
L.G. Littlefield ◽  
R.J. DuFrain ◽  
J.L. Wilmer
Keyword(s):  
Bone Marrow ◽  
Chromosome Aberrations ◽  
Bone Marrow Cells ◽  
Factors Affecting ◽  
Marrow Cells

scholarly journals Chromosome aberrations in T lymphocytes carrying adult T-cell leukemia- associated antigens (ATLA) from healthy adults

Blood ◽  
1983 ◽  
Vol 61 (1) ◽  
pp. 205-207 ◽  
Author(s):  
S Fukuhara ◽  
Y Hinuma ◽  
YI Gotoh ◽  
H Uchino
Keyword(s):  
T Cell ◽  
T Lymphocytes ◽  
Chromosome Aberrations ◽  
Unknown Origin ◽  
Healthy Adults ◽  
T Cell Leukemia ◽  
Cell Leukemia ◽  
Adult T Cell Leukemia ◽  
The Third ◽  
Abnormal Cells

Abstract Chromosomes were studied in cultured T lymphocytes carrying adult T- cell leukemia-associated antigens (ATLA) that were obtained from five Japanese anti-ATLA seropositive healthy adults. Chromosomally abnormal cells were observed in three of the five healthy adults, and these cells were clonal in two subjects. All cells examined in one subject had rearrangements of chromosome nos. 7 and 14. Clonal cells from the second had a minute chromosome of unknown origin. A few cells in the third had nonclonal rearrangements of chromosomes. Thus, ATLA-positive T lymphocytes in some anti-ATLA seropositive healthy people have chromosome aberrations.

On Factors Affecting the Fixation of Chromosomal Rearrangements and Neutral Genes: Computer Simulations

Evolution ◽  
1986 ◽  
Vol 40 (3) ◽  
pp. 625 ◽  
Author(s):  
Ronald K. Chesser ◽  
Robert J. Baker
Keyword(s):  
Computer Simulations ◽  
Chromosomal Rearrangements ◽  
Factors Affecting

Teratogenic effects of direct injection of aqueous and protein-bound trypan blue into the bloodstream of 3-day chick embryos

Teratology ◽  
1970 ◽  
Vol 3 (3) ◽  
pp. 269-272 ◽  
Author(s):  
Stanley Kaplan ◽  
E. Marshall Johnson
Keyword(s):  
Trypan Blue ◽  
Direct Injection ◽  
Chick Embryos ◽  
Teratogenic Effects

Formation and Regeneration of Abnormal Cells of the Marine Diatom Ditylum Brightwellii (West) Grunow

1980 ◽  
Vol 60 (1) ◽  
pp. 243-253 ◽  
Author(s):  
G. S. Canterford
Keyword(s):  
Heavy Metals ◽  
Ethylenediaminetetraacetic Acid ◽  
Considerable Effect ◽  
Marine Diatom ◽  
Factors Affecting ◽  
Ditylum Brightwellii ◽  
Abnormal Cells ◽  
Total Growth ◽  
Size And Morphology ◽  
Toxic Concentrations

Heavy metals, as well as affecting the growth rate and photosynthesis of algae, have a considerable effect on the size and morphology of algal cells (see, for example, Erickson, 1972; Nuzzi, 1972; Davies, 1974; Kayser, 1976). Changes in the colour of algae in the presence of heavy metals have also been observed (Greenfield, 1942). At metal concentrations resulting in total growth inhibition of algae the cells, although abnormal, are not necessarily dead (see, for example, Nielsen & Kamp-Nielsen, 1970; Erickson, 1972; Nuzzi, 1972; Blankenship & Wilbur, 1975).In heavy-metal toxicity studies with Ditylum brightwellii (West) Grunow (Canterford & Canterford, 1979) it was observed that low concentrations of ethylenediaminetetraacetic acid (EDTA) and toxic concentrations of several heavy metals resulted in the formation of abnormal cells. In the present paper factors affecting the formation of abnormal cells are discussed. Experiments were carried out to establish whether or not these abnormal cells could be regenerated.

scholarly journals Is the Incidence of Gastroschisis Increasing Recently?

2018 ◽  
Vol 7 (2) ◽  
pp. 18
Author(s):  
Aditya Pratap Singh ◽  
Arun Kumar Gupta ◽  
Ramesh Tanger ◽  
Vinay Mathur ◽  
Dileep Garg
Keyword(s):  
Mortality Rate ◽  
Abdominal Wall ◽  
Abdominal Wall Defect ◽  
Incidence Rates ◽  
Middle Income ◽  
Wall Defect ◽  
Birth Prevalence ◽  
Factors Affecting ◽  
High Incidence ◽  
Very High

Aims and Objectives: Gastroschisis is a predominantly full thickness right-sided periumbilical abdominal wall defect. We have observed that the GS birth prevalence has more than doubled over the past few decades. The aim of our study is to point out the increasing incidence of the GS.Materials and Methods: We conducted a retrospective study to collect data of the GS, admitted in our institute during the period of 2 years from January 2015 to December 2016. The very high incidence of this congenital anomalies together with the fact that GS is the most common abdominal wall defect requiring neonatal operative intervention and its mortality rate is still very high that prompted us to study its demography, descriptive epidemiology, and quantifying mortality rate and try to analyze factors affecting outcome and cause that might explain this epidemiological pattern. Low- and middle-income countries are often reported to have a higher mortality when compared to patients born in the developed countries.Results: A total of 98 patients of the GS were admitted during the period of 2 years. The mortality and survival rate were 26.5% (26) and mortality 73.4% (72) respectively.Conclusion: Our data demonstrate that in our institution too, recent trends of increasing prevalence of GS are real. Still, we had a high mortality rate. This study highlights the need for further research to find out the causes of such high incidence rates and to improve our outcomes.

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