The effect of streptomycin on cell size of a strain of Pseudomonas aeruginosa

1969 ◽  
Vol 15 (2) ◽  
pp. 209-213
Author(s):  
Frederick Bernheim

Washed cells of a strain of Pseudomonas aeruginosa rapidly decrease in size when they are put in salt solutions and then upon incubation increase in size. In the presence of streptomycin (10 μg/ml) this increase is followed by a decrease but only if ammonia has been assimilated by the cells in the presence of an oxidizable substrate. The extent of the decrease caused by streptomycin is proportional to the amount of ammonia assimilated and also the concentration of the salt solution. Chlortetracycline (0.02 μg/ml), chloramphenicol (1.0 μg/ml), and cycloserine (10 μg/ml) completely inhibit the streptomycin effect when added before or after ammonia assimilation is complete.


Soft Matter ◽  
2018 ◽  
Vol 14 (29) ◽  
pp. 6058-6069 ◽  
Author(s):  
Yael Avni ◽  
Tomer Markovich ◽  
Rudolf Podgornik ◽  
David Andelman

We revisit the charge-regulation mechanism of macro-ions and apply it to mobile macro-ions in a bathing salt solution.





1972 ◽  
Vol 18 (11) ◽  
pp. 1643-1646
Author(s):  
Frederick Bernheim

When washed suspensions of Pseudomonas aeruginosa are placed in salt solutions, the cells shrink rapidly and then swell more slowly as salt and water enter them. The rate of swelling is increased by EDTA and its calcium chelate. The effect of EDTA is antagonized by diamines; the effect of the calcium chelate by carboxylic acids. The relative effects of EDTA and the calcium chelate differ in different salts which suggests different sites of action. The evidence indicates that EDTA does not act by chelating calcium or magnesium in the membrane of these cells but that its two nitrogen atoms combine by electrostatic forces with anionic sites, and the chelate, in which the nitrogen atoms are bound to calcium, combines by the same forces to cationic sites. Barium, strontium, and magnesium chelates are inactive.



1985 ◽  
Vol 50 ◽  
Author(s):  
A. R. Flambard ◽  
H.-U. Fusban ◽  
Ch. Keiling ◽  
G. Marx

AbstractExperiments have been carried out into the leaching of ILW conditioned in cement and bitumen in concentrated salt solutions. Although difficult to observe with real waste leachates, investigations into the leaching of a simulated waste in cement have indicated leached transuranic levels of ca. 10−9 M ; the amount of activity leached by a quinary salt solution being 102 - 103 times higher . This has been interpreted in terms of a pH effect. For the real waste, spectroscopy has indicated a significantly larger release of Cs from cement than from bitumen. For all waste samples a notable absence of colloidal material was observed ; an observation which can be explained in terms of the high solution ionic strengths and the corresponding influence upon radionuclide solvation.Transuranic mobility studies through salt and sand from a salt dome in Northern Germany have shown the presence of at least two types of species of wildly differing mobility ; one migrating with approximately the same velocity as that of the solvent front and the other strongly retarded. Actinide recoveries (i.e. that passing through the columns) could be strongly influenced by either changing the system pH or by the addition of a competitor such as Ce ; the latter effect pointing to a competitive sorption.



1980 ◽  
Vol 124-124 (2-3) ◽  
pp. 197-203 ◽  
Author(s):  
Dick B. Janssen ◽  
Huub J. M. op den Camp ◽  
Pieter J. M. Leenen ◽  
Chris van der Drift


1926 ◽  
Vol 8 (4) ◽  
pp. 317-337 ◽  
Author(s):  
John H. Northrop ◽  
M. Kunitz

1. The swelling and the osmotic pressure of gelatin at pH 4.7 have been measured in the presence of a number of salts. 2. The effect of the salts on the swelling is closely paralleled by the effect on the osmotic pressure, and the bulk modulus of the gelatin particles calculated from these figures is constant up to an increase in volume of about 800 per cent. As soon as any of the salts increase the swelling beyond this point, the bulk. modulus decreases. This is interpreted as showing that the elastic limit has been exceeded. 3. Gelatin swollen in acid returns to its original volume after removal of the acid, while gelatin swollen in salt solution does not do so. This is the expected result if, as stated above, the elastic limit had been exceeded in the salt solution. 4. The modulus of elasticity of gelatin swollen in salt solutions varies in the same way as the bulk modulus calculated from the osmotic pressure and the swelling. 5. The increase in osmotic pressure caused by the salt is reversible on removal of the salt. 6. The observed osmotic pressure is much greater than the osmotic pressure calculated from the Donnan equilibrium except in the case of AlCl3, where the calculated and observed pressures agree quite closely. 7. The increase in swelling in salt solutions is due to an increase in osmotic pressure. This increase is probably due to a change in the osmotic pressure of the gelatin itself rather than to a difference in ion concentration.



2020 ◽  
Vol 71 (8) ◽  
pp. 148-158
Author(s):  
Kamisah D. Pandiangan ◽  
Wasinton Simanjuntak ◽  
R. Supriyanto ◽  
Ilim Ilim ◽  
Ponco Prasetyo ◽  
...  

This study was conducted to explore the production of magnesium oxide from raw salt solution using electrochemical precipitation, followed by calcination. Electrochemical precipitation was conducted by electrolysis of the salt solution using nickel rods as cathodes and graphite as anodes. Two sets of salt solutions were prepared: one set without pretreatment and another with BaCl2 pretreatment. The solutions were used to study the effect of salt concentration, potential, and electrolysis time. The representatives of the MgO produced were tested as catalysts for transesterification of coconut oil. The results indicate that optimum mass of precipitate was produced from 400 mg/L salt solution electrolyzed using 8 V for 60 min. Elemental analysis using X-Ray Fluorescence (XRF) revealed the presence of Mg as the main component of the precipitate, confirming the electrochemical conversion of Mg2+ into solid Mg(OH)2. The MgO with the purity of 74.23% and 88.87% was produced from non-pretreated and pretreated salt solution, respectively. The transesterification experiments indicate that the yield of 90% and 98% was achieved using the MgO produced from non-pretreated and pretreated salt solution, respectively.



2020 ◽  
Vol 8 (3) ◽  
Author(s):  
Sooraj Shiby ◽  
Nammi Srinagalakshmi ◽  
Nilesh J. Vasa ◽  
Shigeki Matsuo ◽  
Muralidhar Miryala

Abstract The influence of a subnanosecond pulsed laser-based scribing of copper (Cu) and aluminum (Al) in salt solutions (NaCl and KCl) on the formation of microchannels is reported. This technique allows laser scribing along with selective etching of Cu and Al thin films. The focused laser beam can elevate the surface temperature on the sample and hence the chemical reaction rate, resulting in combined ablation with selective-area etching. The depth of microchannels in Cu and Al films is increased by 3–5 μm using the proposed hybrid technique. The average surface roughness values in the microchannel are decreased compared to that of scribing in water and air. The hybrid approach of laser-based scribing combined with electrochemical etching in neutral salt solutions allows uniform channel with almost no redeposit layer and debris on the channel edges. Further, an approach wherein, an application of direct current (DC) voltage (1.2 V) between the tool and the workpiece while laser scribing of Cu and Al in salt solution was demonstrated to improve the channel depth by few micrometers. This hybrid machining technique has also resulted in a reduction in the surface oxidation near the laser-ablated zone compared to that observed in air and water-based experiments.



Soil Research ◽  
1983 ◽  
Vol 21 (1) ◽  
pp. 105 ◽  
Author(s):  
NS Jayawardane

Hydraulic conductivities of Pachappa and Waukena soils for different salt solutions, predicted by the equivalent salt solution methods, showed satisfactory agreement with measured values. The values of equivalent salt solutions for these soils were calculated from macroscopic swelling values of extracted clays. The equivalent salt solution method and the simplified domain model method of McNeal predicted equally accurately, the changes in hydraulic conductivities of Pachappa soil in salt solutions with sodium absorption ratio values from 15 to 100.



Soil Research ◽  
1979 ◽  
Vol 17 (1) ◽  
pp. 89 ◽  
Author(s):  
NS Jayawardane ◽  
JA Beattie

A pore size index was used to express quantitatively the relative decrease in size of common pore of confined columns of krasnozem, red-brown and alluvial soils, with reduction in electrolyte concentration of percolating solutions of a given sodium absorption ratio (SAR). The pore size index of red-brown and alluvial soils decreased markedly with reduction in electrolyte concentration, especially in solutions of high SAR. In the krasnozem soil the pore size index remained fairly constant for a wide range of salt solutions. Moisture release curves of red-brown soil for solutions of decreasing electrolyte concentration and constant SAR and for solutions of increasing SAR and constant electrolyte concentration can be used in combination to obtain moisture release curves of this soil for solutions at any intermediate values of SAR and electrolyte concentration. They also reveal the pattern of volume change of different sized pores.



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