The variations in the antigenic composition of Mycobacterium tuberculosis during the growth cycle as measured by the passive hemagglutination and precipitation reactions

1969 ◽  
Vol 15 (7) ◽  
pp. 681-688
Author(s):  
R. Turcotte
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Ammonium Sulfate ◽  
Culture Filtrate ◽  
Growth Cycle ◽  
Agar Gel ◽  
Passive Hemagglutination ◽  
Antigenic Composition ◽  
Hemagglutination Test ◽  
Culture Filtrates ◽  
Precipitation Reactions

The erythrocyte-sensitizing ability (ESA), as measured by the bis-diazotized-benzidine hemagglutination test, was determined for the extractable and culture filtrate antigens of three strains of mycobacteria (H37Rv, H37Ra, and BCG). The ESA of the precipitated and supernatant fractions obtained from these preparations by precipitation with ammonium sulfate, was found to vary according to the age of the cultures: the concentration of antigens decreased in the bacillary extracts and increased in the corresponding culture filtrates with the aging of the mycobacterial cultures.The variation in the antigenic composition of bacterial extracts and of culture filtrates during the growth cycle was also demonstrated by immunodiffusion techniques in agar gel. However, all extractable antigens of a given Mycobacterium did not appear in the culture filtrates.

Passive hemagglutination test and its application for detection of anti-DNA antibody in NZB/W F1 mice administered methyl-B12

Ensho ◽  
1982 ◽  
Vol 2 (4) ◽  
pp. 437-438
Author(s):  
Hiromi Sugiyama ◽  
Jun-ichi Isomura ◽  
Tomohisa Ikeda ◽  
Giichi Takimoto ◽  
Yutaka Mizushima
Keyword(s):  
Passive Hemagglutination ◽  
Hemagglutination Test

scholarly journals CHEMICAL INVESTIGATIONS ON THE ACTIVE PRINCIPLES OF THE PHENOMENON OF LOCAL SKIN REACTIVITY TO BACTERIAL FILTRATES

1938 ◽  
Vol 67 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Gregory Shwartzman ◽  
S. A. Morell
Keyword(s):  
Particle Size ◽  
Physicochemical Properties ◽  
Ammonium Sulfate ◽  
Culture Filtrate ◽  
Active Materials ◽  
Local Skin ◽  
Skin Reactivity ◽  
Average Porosity ◽  
Active Substances ◽  
Capillary Analysis

Several physicochemical properties of the active principles of the phenomenon of local skin reactivity to bacterial filtrates have been investigated. Ultrafiltration through Zsigmondy filters of graded porosities has shown that the active substances are retained by membranes finer than 100 to 120 seconds, whereas coarser ones readily permit their passage. The average porosity of this filtration end-point represents a particle size of about 50 to 100 mµ. When fractionally precipitated with ammonium sulfate, most of the activity of a culture filtrate was concentrated in the two-thirds saturated portion. Isoelectric properties were studied by means of capillary analysis and cataphoresis. At pH 3.0 and below, the substances suspended in the culture filtrates migrated to the cathode; activity in this chamber, however, could not be demonstrated. At pH 4.0 and above, reversal to the anode occurred, as the active materials became negatively charged and readily migrated to this chamber. The isoelectric point, therefore, was considered to be between pH 3.0 and 4.0. Preliminary experiments on adsorption, extraction, and pH stability have been described.

scholarly journals Vaccine-Elicited 10-Kilodalton Culture Filtrate Protein-Specific CD8+ T Cells Are Sufficient To Mediate Protection against Mycobacterium tuberculosis Infection

2008 ◽  
Vol 76 (5) ◽  
pp. 2249-2255 ◽  
Author(s):  
Ying Wu ◽  
Joshua S. Woodworth ◽  
Daniel S. Shin ◽  
Sheldon Morris ◽  
Samuel M. Behar
Keyword(s):  
T Cells ◽  
Mycobacterium Tuberculosis ◽  
Culture Filtrate ◽  
Protective Immunity ◽  
Protective Antigen ◽  
T Cell Response ◽  
Rapid Expansion ◽  
Mycobacterium Tuberculosis Infection ◽  
Infected People ◽  
Culture Filtrate Protein

ABSTRACT The 10-kDa culture filtrate protein (CFP-10) and 6-kDa early secretory antigen of T cells (ESAT-6) are secreted in abundance by Mycobacterium tuberculosis and are frequently recognized by T cells from infected people. The genes encoding these proteins have been deleted from the genome of the vaccine strain Mycobacterium bovis bacillus Calmette-Guérin (BCG), and it is hypothesized that these proteins are important targets of protective immunity. Indeed, vaccination with ESAT-6 elicits protective CD4+ T cells in C57BL/6 mice. We have previously shown that M. tuberculosis infection of C3H mice elicits CFP-10-specific CD8+ and CD4+ T cells. Here we demonstrate that immunization with a CFP-10 DNA vaccine stimulates a specific T-cell response only to the H-2Kk-restricted epitope CFP-1032-39. These CFP-1032-39-specific CD8+ cells undergo a rapid expansion and accumulate in the lung following challenge of immunized mice with aerosolized M. tuberculosis. Protective immunity is induced by CFP-10 DNA vaccination as measured by a CFU reduction in the lung and spleen 4 and 8 weeks after challenge with M. tuberculosis. These data demonstrate that CFP-10 is a protective antigen and that CFP-1032-39-specific CD8+ T cells elicited by vaccination are sufficient to mediate protection against tuberculosis.

scholarly journals Comparative Analysis of B- and T-Cell Epitopes of Mycobacterium leprae and Mycobacterium tuberculosis Culture Filtrate Protein 10

2004 ◽  
Vol 72 (6) ◽  
pp. 3161-3170 ◽  
Author(s):  
John S. Spencer ◽  
Hee Jin Kim ◽  
Angela M. Marques ◽  
Mercedes Gonzalez-Juarerro ◽  
Monica C. B. S. Lima ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
T Cell ◽  
Culture Filtrate ◽  
Mycobacterium Leprae ◽  
Cross Reactivity ◽  
Interferon Response ◽  
Positive Staining ◽  
T Cell Epitopes ◽  
Peptide Epitopes ◽  
Culture Filtrate Protein

ABSTRACT Culture filtrate protein 10 (CFP-10) from Mycobacterium tuberculosis is a well-characterized immunodominant 10-kDa protein antigen known to elicit a very potent early gamma interferon response in T cells from M. tuberculosis-infected mice and humans. The sequence of the Mycobacterium leprae homologue of CFP-10 shows only 40% identity (60% homology) at the protein level with M. tuberculosis CFP-10 and thus has the potential for development as a T- or B-cell reactive antigen for specific diagnosis of leprosy. Antisera raised in mice or rabbits against recombinant M. leprae and M. tuberculosis CFP-10 proteins reacted only with homologous peptides from arrays of overlapping synthetic peptides, indicating that there was no detectable cross-reactivity at the antibody level. Sera from leprosy and tuberculosis patients were also specific for the homologous protein or peptides and showed distinct patterns of recognition for either M. leprae or M. tuberculosis CFP-10 peptides. At the cellular level, only 2 of 45 mouse T-cell hybridomas raised against either M. leprae or M. tuberculosis CFP-10 displayed a cross-reactive response against the N-terminal heterologous CFP-10 peptide, the region that exhibits the highest level of identity in the two proteins; however, the majority of peptide epitopes recognized by mouse T-cell hybridomas specific for each protein did not cross-react with heterologous peptides. Coupled with the human serology data, these results raise the possibility that peptides that could be used to differentiate infections caused by these two related microorganisms could be developed. Immunohistochemical staining of sections of M. leprae-infected nude mouse footpads resulted in strongly positive staining in macrophages and dendritic cells, as well as weaker staining in extracellular areas, suggesting that M. leprae CFP-10, like its homologue in M. tuberculosis, is a secreted protein.

Serological differentiation of the Ascochyta species on peas

1968 ◽  
Vol 14 (4) ◽  
pp. 449-451 ◽  
Author(s):  
C. Madhosingh ◽  
V. R. Wallen
Keyword(s):  
Immune Serum ◽  
Complex Pattern ◽  
Serological Relationship ◽  
Agar Gel ◽  
Ascochyta Pinodes ◽  
Ascochyta Pisi ◽  
Precipitation Reactions ◽  
Species Specific ◽  
Specific Reactions

Specific immune serum against Ascochyta pisi was developed and three species-specific reactions were obtained by standard absorption techniques using extracts of both Ascochyta pinodella and Ascochyta pinodes. Ouchterlony tests showed a complex pattern of precipitation reactions in agar gel between the antigens from the three species and antisera developed in response to them and indicated distinct serological relationships among the three fungi. The pattern of reciprocal precipitin reactions indicates a closer serological relationship between A. pinodella and A. pinodes than between either of these two species and A. pisi. Two serologically similar antigenic components were present in the extracts from the three species.

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