Factors affecting the production of m-cresol by Valsa friesii

1970 ◽  
Vol 16 (7) ◽  
pp. 587-593
Author(s):  
J. J. Child ◽  
R. H. Haskins
Keyword(s):  
Culture Filtrate ◽  
Colorimetric Assay ◽  
Defined Medium ◽  
Distilled Water ◽  
Shake Culture ◽  
Chemically Defined Medium ◽  
Factors Affecting ◽  
High Yields

A chemically defined medium for the cultivation of Valsa friesii Duby (Fuckel) PRL 1736 has been developed and conditions established for accumulation of high yields of m-cresol in the culture filtrate. Yields of 1 g per liter as determined by colorimetric assay using 4-aminoantipyrine are obtained after 10 days' incubation in shake culture at 28 °C in the absence of light on a medium containing glucose, 15 g; alanine, 2 g; K2HPO4, 0.3 g; KH2PO4, 0.2 g; salts (0.2 g MgSO4∙7H2O, 0.1 g NaCl, 0.1 g CaCl2, 0.015 g FeSO4, and 0.25 mg ZnCl2); and vitamins (4 mg each of thiamine HCl, pyridoxin HCl, 10 mg inositol, and 10 μg biotin) per liter of distilled water. The organism is shown to tolerate higher concentrations of m-cresol than do eight other fungi tested.

Chemically defined medium for high yields of sterigmatocystin

1982 ◽  
Vol 78 (2) ◽  
pp. 99-105 ◽  
Author(s):  
H. Hitokoto ◽  
S. Morozumi ◽  
T. Wauke ◽  
S. Sakai ◽  
S. Yoshikawa
Keyword(s):  
Defined Medium ◽  
Chemically Defined Medium ◽  
High Yields

scholarly journals Partial characterization of alkaline proteases

2014 ◽  
Vol 11 (1) ◽  
pp. 17-21 ◽  
Author(s):  
Bogusław Narzymski ◽  
Jadwiga Chmielnicka
Keyword(s):  
Culture Filtrate ◽  
Proteolytic Enzymes ◽  
Defined Medium ◽  
Partial Characterization ◽  
Alkaline Proteases ◽  
Chemically Defined Medium

When <i>Mucor Microsporus</i> Nam. was grown in a chemically defined medium alkaline proteases produced by this organisms appeares in the culture filtrate. Crude proteolytic enzymes preparations isolated from these filtrates show two optima for thedigestion of casein, namely at pH 8 and 11.

Nutritional factors affecting the synthesis of an antiphagocytic factor by group E streptococci

1984 ◽  
Vol 30 (3) ◽  
pp. 406-411 ◽  
Author(s):  
G. E. Wessman
Keyword(s):  
Molecular Weight ◽  
Defined Medium ◽  
Chemically Defined Medium ◽  
Nutritional Factors ◽  
Factors Affecting ◽  
Cultural Medium ◽  
Molecular Weight Cutoff ◽  
Tryptose Phosphate Broth ◽  
Nominal Molecular Weight ◽  
Cells Cultured

Effects of components of the cultural medium on formation of a group E streptococcal antiphagocytic factor (APF), as detected by an indirect bactericidal test, were studied. Bovine serum albumin (BSA) replaced serum in promoting synthesis of APF in a chemically defined medium (CDM), Todd–Hewitt broth (THB), or tryptose phosphate broth (TPB). Stimulatory factors in BSA were not retained by diafiltration membranes of nominal molecular weight cutoff limits of 10 000 or greater. Citrate, lactate, pyruvate, or oleate, often found in BSA, could not replace BSA in stimulating synthesis of APF. Cells cultured in THB were more resistant to phagocytosis by porcine leukocytes than those grown in CDM or TPB, and the addition of varying amounts of THB to CDM stimulated a measurable response in synthesis of APF. Specific substrates caused different rates of APF synthesis; in decreasing order of effectiveness were glucose or mannose, sucrose, fructose, and trehalose. Proteolytic activity, which might cause the production of phagocyte-sensitive cells by destroying APF activity during culture, was not detectable in significant amounts in subcultures of the age employed in bactericidal tests.

scholarly journals Factors Affecting Exocellular Polysaccharide Production by Lactobacillus delbrueckii subsp.bulgaricus Grown in a Chemically Defined Medium

2000 ◽  
Vol 66 (8) ◽  
pp. 3427-3431 ◽  
Author(s):  
Sandrine Petry ◽  
Sylviane Furlan ◽  
Marie-Jeanne Crepeau ◽  
Jutta Cerning ◽  
Michel Desmazeaud
Keyword(s):  
Carbon Source ◽  
Defined Medium ◽  
Lactobacillus Delbrueckii ◽  
Composition Analysis ◽  
Chemically Defined Medium ◽  
Fermentation Conditions ◽  
Factors Affecting ◽  
Exocellular Polysaccharide ◽  
The Individual ◽  
Ph Controlled

ABSTRACT We developed a chemically defined medium (CDM) containing lactose or glucose as the carbon source that supports growth and exopolysaccharide (EPS) production of two strains ofLactobacillus delbrueckii subsp. bulgaricus. The factors found to affect EPS production in this medium were oxygen, pH, temperature, and medium constituents, such as orotic acid and the carbon source. EPS production was greatest during the stationary phase. Composition analysis of EPS isolated at different growth phases and produced under different fermentation conditions (varying carbon source or pH) revealed that the component sugars were the same. The EPS from strain L. delbrueckii subsp.bulgaricus CNRZ 1187 contained galactose and glucose, and that of strain L. delbrueckii subsp. bulgaricusCNRZ 416 contained galactose, glucose, and rhamnose. However, the relative proportions of the individual monosaccharides differed, suggesting that repeating unit structures can vary according to specific medium alterations. Under pH-controlled fermentation conditions, L. delbrueckii subsp. bulgaricusstrains produced as much EPS in the CDM as in milk. Furthermore, the relative proportions of individual monosaccharides of EPS produced in pH-controlled CDM or in milk were very similar. The CDM we developed may be a useful model and an alternative to milk in studies of EPS production.

Improved cryopreservation of domestic cat sperm in a chemically defined medium

2012 ◽  
Vol 78 (9) ◽  
pp. 2120-2128 ◽  
Author(s):  
M.M. Vick ◽  
H.L. Bateman ◽  
C.A. Lambo ◽  
W.F. Swanson
Keyword(s):  
Defined Medium ◽  
Domestic Cat ◽  
Chemically Defined Medium
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