Improved recovery of fecal coliforms from the Ottawa River by membrane filters in the presence of food debris

1985 ◽  
Vol 31 (1) ◽  
pp. 16-18 ◽  
Author(s):  
Jeffrey M. Farber ◽  
Anthony N. Sharpe ◽  
Miloslav Kalab
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
River Water ◽  
Water Samples ◽  
Fecal Coliform ◽  
Fecal Coliforms ◽  
Normal Stresses ◽  
Membrane Filters ◽  
Ottawa River ◽  
Scanning Electron

In the absence of food debris, Sartorius and Millipore HA filters recovered substantially fewer fecal coliforms from Ottawa River water than did Millipore HC. On addition of a small quantity of sterile blended carrot to water samples, recovery by the poorer filters equalled that on Millipore HC. Scanning electron microscopy revealed bacteria sheltered in crevices formed by carrot fibres and thus protected from the normal stresses of exposure. Addition of carrot debris (e.g., 0.03 g carrot to 100 mL of sample) thus provides a convenient and inexpensive means of reducing variations in fecal coliform recovery between brands of membrane filters.

The Distribution of Diatom in the Five Lakes along the Yangtze River

2013 ◽  
Vol 850-851 ◽  
pp. 1309-1312
Author(s):  
Yu Zhou ◽  
Quan Yu ◽  
Wei Hang ◽  
Yu Zhong Wang ◽  
Hui Pin Wang ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Water Samples ◽  
Yangtze River ◽  
Water Bodies ◽  
The Yangtze River ◽  
Significant Difference ◽  
The Future ◽  
Scanning Electron

In order to investigate the diatom distribution in the five lakes along the Yangtze River, and discuss the significance for the diagnosis of drowning. 6 water samples were collected in the five lakes along Yangtze River in June, 2012. Subsequently, The genus and the quantity of the diatoms in the water samples were investigated using scanning electron microscopy (SEM). The result showed us that there was significant difference between the waters sampled from the different sites . These data on diatom distribution are helpful to the diagnosis of the cause of the death as well as to the determination of the drowning site in cases of drowning. Therefore, it is necessary to develop the database of diatoms in more extensive water bodies for the forensic diagnosis of drowning in the future.

Effect of Voltage on the Energy Dispersive Microanalysis of Single Asbestos Fibers in the SEM

1974 ◽  
Vol 32 ◽  
pp. 530-531
Author(s):  
John M. Wehrung ◽  
Richard J. Harniman ◽  
James H. McAlear
Keyword(s):  
Public Health ◽  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
High Resolution ◽  
Water Samples ◽  
Health Hazard ◽  
Asbestos Fibers ◽  
Energy Dispersive Microanalysis ◽  
Asbestos Contamination ◽  
Scanning Electron

Asbestos contamination and subsequent inhalation and ingestion is becoming recognized as a substantial public health hazard. High resolution scanning electron microscopy has been employed to evaluate air and water samples for the occurence and nature of asbestos fiber contamination. This method has many advantages over the more time consuming and costly TEM methods with equal capability of resolving elemental fibrils (fig. 1).

Diatom Distributions in the Eastern Yangtze River and the Main Lakes, Jiangsu Province, China, and their Significance for the Forensic Diagnosis of Drowning

2013 ◽  
Vol 790 ◽  
pp. 546-549
Author(s):  
Yu Zhou ◽  
Yu Zhong Wang ◽  
Jian Wen Huang ◽  
Hui Pin Wang ◽  
Sun Lin Hu ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Water Samples ◽  
Yangtze River ◽  
Jiangsu Province ◽  
Water Bodies ◽  
Significant Difference ◽  
The Future ◽  
Scanning Electron

In the presented paper, diatom distributions in the eastern Yangtze River and the main lakes, Jiangsu Province, China, were investigated, and on this basis, its significance for the diagnosis of drowning was discussed as well. Totally, 14 water samples were collected in 5 sites along the eastern Yangtze River and in 3 lakes within Jiangsu province of China in June and September, 2012. The genus and the quantity of the diatoms in the water samples were subsequently investigated using scanning electron microscopy (SEM). With reference to diatom distribution, there is significant difference between the waters sampled from the different sites and in the different months. These data on diatom distribution are helpful to the diagnosis of the cause of the death as well as to the determination of the drowning site in cases of drowning. Therefore, it is necessary to develop the database of diatoms in more extensive water bodies for the forensic diagnosis of drowning in the future.

Pathogenesis of Human Hair Defects

1974 ◽  
Vol 32 ◽  
pp. 12-13
Author(s):  
P.S. Porter ◽  
T. Aoyagi ◽  
R. Matta
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Human Hair ◽  
Standard Techniques ◽  
Scanning Electron

Using standard techniques of scanning electron microscopy (SEM), over 1000 human hair defects have been studied. In several of the defects, the pathogenesis of the abnormality has been clarified using these techniques. It is the purpose of this paper to present several distinct morphologic abnormalities of hair and to discuss their pathogenesis as elucidated through techniques of scanning electron microscopy.

Ultrastructural Analysis of the Salivary Glands of Gromphadorhina Portentosa (Schaum)

1977 ◽  
Vol 35 ◽  
pp. 638-639
Author(s):  
P.J. Dailey
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Salivary Glands ◽  
Secretory Vesicles ◽  
The Past ◽  
Transmission Electron ◽  
Means Of Transmission ◽  
Gromphadorhina Portentosa ◽  
Scanning Electron ◽  
Topographical Relief

The structure of insect salivary glands has been extensively investigated during the past decade; however, none have attempted scanning electron microscopy (SEM) in ultrastructural examinations of these secretory organs. This study correlates fine structure by means of SEM cryofractography with that of thin-sectioned epoxy embedded material observed by means of transmission electron microscopy (TEM).Salivary glands of Gromphadorhina portentosa were excised and immediately submerged in cold (4°C) paraformaldehyde-glutaraldehyde fixative1 for 2 hr, washed and post-fixed in 1 per cent 0s04 in phosphosphate buffer (4°C for 2 hr). After ethanolic dehydration half of the samples were embedded in Epon 812 for TEM and half cryofractured and subsequently critical point dried for SEM. Dried specimens were mounted on aluminum stubs and coated with approximately 150 Å of gold in a cold sputtering apparatus.Figure 1 shows a cryofractured plane through a salivary acinus revealing topographical relief of secretory vesicles.

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