Hybrid biovars of Agrobacterium species isolated from conifer roots

1991 ◽  
Vol 37 (1) ◽  
pp. 34-41 ◽  
Author(s):  
C. R. Bell ◽  
W. D. Ramey

A total of 377 heterotrophic bacteria were isolated on nonselective medium from the rhizoplanes of five species of conifer. The species were western hemlock (Tsuga heterophylla), Douglas-fir (Pseudotsuga menziesii), lodgepole pine (Pinus contorta), white spruce (Picea glauca), and western red cedar (Thuja plicata). Twenty-eight strains from this population were identified as presumptive agrobacteria. All proved nontumourigenic. Principal-component analysis indicated that the strains, which had clustered into two discrete groups, had intermediate biovar characteristics. Cluster 1 was predominately biovar 3/2 in character, cluster 2 was predominately biovar 2/3. All the presumptive agrobacteria were distributed randomly with respect to the tree species. This study demonstrates that agrobacteria, although atypical, do occur in forest soils and attests to the ubiquity of the genus in soil. Key words: Agrobacterium, biovars, crown gall, conifers.

2001 ◽  
Vol 79 (4) ◽  
pp. 389-397 ◽  
Author(s):  
Hugh J Barclay

Leaf angle distributions are important in assessing both the flexibility of a plant's response to differing daily and seasonal sun angles and also the variability in the proportion of total leaf area visible in remotely sensed images. Leaf angle distributions are presented for six conifer species, Abies grandis (Dougl. ex D. Don) Lindl., Thuja plicata Donn. ex D. Don, Tsuga heterophylla (Raf.) Sarg., Pseudotsuga menziesii (Mirb.) Franco, Picea sitchensis (Bong.) Carr. and Pinus contorta Dougl. ex Loud. var. latifolia. The leaf angles were calculated by measuring four foliar quantities, and then the distributions of leaf angles are cast in three forms: distributions of (i) the angle of the long axis of the leaf from the vertical for the range 0–180°; (ii) the angle of the long axis of the leaf for the range 0–90°; and (iii) the angle of the plane of the leaf for the range 0–90°. Each of these are fit to the ellipsoidal distribution to test the hypothesis that leaf angles in conifers are sufficiently random to fit the ellipsoidal distribution. The fit was generally better for planar angles and for longitudinal angles between 0° and 90° than for longitudinal angles between 0° and 180°. The fit was also better for Tsuga heterophylla, Pseudotsuga menziesii, Picea sitchensis, and Pinus contorta than for Abies grandis and Thuja plicata. This is probably because Abies and Thuja are more shade tolerant than the other species, and so the leaves in Abies and Thuja are preferentially oriented near the horizontal and are much less random than for the other species. Comparisons of distributions on individual twigs, whole branches, entire trees, and groups of trees were done to test the hypothesis that angle distributions will depend on scale, and these comparisons indicated that the apparent randomness and goodness-of-fit increased on passing to each larger unit (twigs up to groups of trees).Key words: conifer, leaf angles, ellipsoidal distribution.


1983 ◽  
Vol 20 (5) ◽  
pp. 873-885 ◽  
Author(s):  
Linda E. Heusser

Varved, black clayey silts deposited in the marine waters of Saanich Inlet yield unusually abundant and diverse pollen assemblages derived from the coastal Douglas-fir (Pseudotsuga) and western hemlock (Tsuga heterophylla) forests of southwestern British Columbia. The 12 000 year palynological record chronicles the development of vegetation since ice left Saanich Inlet: the succession of pine (Pinus contorta) and alder (Alnus rubra) woodlands by forests characterized by Douglas-fir and oak (Quercus) and later by western hemlock and red cedar (Thuja plicata). Rapid deposition of annual layers of pollen, charcoal, and other terrigenous particles provides detailed evidence of changes in land use during the past few hundred years: settlement, logging, farming, and urbanization. Vegetational and climatic changes inferred from pollen spectra in the marine sediments of Saanich Inlet compare favorably with changes inferred from correlative pollen assemblages previously described from adjacent parts of Vancouver Island and the Fraser River valley.


1989 ◽  
Vol 4 (1) ◽  
pp. 10-13 ◽  
Author(s):  
Andrew C. Mason ◽  
David L. Adams

Abstract Bear damage was at least five times higher in thinned blocks than in adjacent unthinned blocks of western larch (Larix occidentalis), lodgepole pine (Pinus contorta), and Engelmann spruce (Picea engelmannii) on the Kootenai National Forest in northwest Montana. Western larch suffered the greatest damage (63% of all trees damaged and 92% of the trees killed). Damaged larch ranged from 4 to 13 in. dbh; the 4 to 8-in. dbh class accounted for 85% of the damage. Douglas-fir (Pseudotsuga menziesii), western redcedar (Thuja plicata), subalpine fir (Abies lasiocarpa), western white pine (Pinus monticola), and western hemlock (Tsuga heterophylla) were not damaged. Stand projections showed up to a 17% reduction in board-foot yield from bear damage, after 50 years, compared with hypothetical undamaged stands. West. J. Appl. For. 4(1):10-13, January 1989.


1993 ◽  
Vol 71 (8) ◽  
pp. 1087-1092
Author(s):  
Salim N. Silim ◽  
Robert D. Guy ◽  
Denis P. Lavender

Short-term effects of the plant growth retardant mefluidide on drought resistance were investigated in seedlings of western red cedar (Thuja plicata Donn), yellow cedar (Chamaecyparis nootkatensis D. Don), and white spruce (Picea glauca (Moench) Voss). Mefluidide was applied as a root drench at concentrations of 0, 0.1, and 0.4 mg∙L−1. Following this, seedlings were stressed by withholding water for a period of up to 7 days and then watering regularly thereafter. Stomatal conductance of the mefluidide-treated seedlings decreased significantly and remained lower than the control seedlings throughout the stress period. As a result of the decreased conductance, shoot water potentials in mefluidide-treated seedlings remained higher throughout the stress period. There was a decrease in net photosynthesis that was partially caused by stomatal limitations, the severity of which depended upon the concentration of mefluidide applied. Mefluidide treatment also resulted in an accumulation of abscisic acid in all three species, and this may have been responsible for the effects on stomatal conductance and water relations. In contrast, the mefluidide-treated seedlings had the highest rates of photosynthesis after rewatering, resulting primarily from a promotion of stomatal opening. Key words: stomatal conductance, water potential, photosynthesis, plant growth inhibitors, abscisic acid, cold hardiness.


1981 ◽  
Vol 113 (4) ◽  
pp. 337-340 ◽  
Author(s):  
W. W. Nijholt ◽  
L. H. McMullen ◽  
L. Safranyik

AbstractPine oil, a by-product of sulphate wood pulping, protected pheromone-baited, living Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco), lodgepole pine (Pinus contorta Dougl.), and spruce (Picea glauca (Moench) Voss - P. engelmannii Parry hybrids) from attack by Douglas-fir beetle (Dendroctonus pseudotsugae Hopk.), mountain pine beetle (D. ponderosa Hopk.), and spruce beetle (D. rufipennis (Kirby)), respectively. Pine oil also protected surrounding trees and reduced attack incidence on Douglas-fir, lodgepole pine, and spruce within at least a 10 m radius. α-Terpineol, one of the constituents of the pine oil mixture, was less effective.


1999 ◽  
Vol 29 (2) ◽  
pp. 274-279
Author(s):  
Raymond C Shearer ◽  
Jack A Schmidt

In 1974, two clearcuts, two shelterwoods, and two sets of eight group selections (equally divided between two elevation zones) were harvested on the Coram Experimental Forest in northwestern Montana. Four levels of tree and residue utilization were compared. Moist fuels on approximately half of each area were poorly burned by prescribed fires in September 1975. Natural regeneration on these treatments was compared in 1979, 1987, and 1992. Regeneration of western larch (Larix occidentalis Nutt.) began in 1975 on soil exposed during yarding of logs and continued mostly in 1977 and 1979 on these scarified sites and other burned areas. Competing vegetation curtailed establishment of larch seedlings much past 1979 on these sites. Few Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) regenerated before 1979 but aggressively established through 1992. Engelmann spruce (Picea engelmannii Parry) and subalpine fir (Abies lasiocarpa (Hook.) Nutt) regeneration began in 1979 and is increasing slowly throughout the area. Western hemlock (Tsuga heterophylla (Raf.) Sarg.) and western red cedar (Thuja plicata Donn.) also slowly regenerate moister areas of the lower elevation units.


1994 ◽  
Vol 72 (11) ◽  
pp. 1635-1646 ◽  
Author(s):  
X. J. Li ◽  
P. J. Burton ◽  
C. L. Leadem

Pregermination stratification treatment was generally more important than the effects of light on seed germination by 14 conifer species and varieties native to British Columbia. Nevertheless, there were some strong species differences in the response of germination to light. Final germination percentage after 21 days (28 days for Abies spp.) for both stratified and unstratified seeds of Picea glauca, Picea sitchensis, and Tsuga heterophylla showed no response to light during germination. Seed germination by Abies grandis, Pinus contorta var. contorta, Pinus contorta var. latifolia, Pinus ponderosa, Pseudotsuga menziesii var. glauca, and Pseudotsuga menziesii var. menziesii responded positively to light if unstratified but was not significantly affected by light when stratified. For Thuja plicata seeds, germination responded positively to light regardless of stratification pretreatment. Light appeared to reduce germination of stratified seeds of Abies amabilis, Abies lasiocarpa, Larix occidentalis, and Pinus monticola, although stratification conditions for these species were suboptimal. The germination rate of stratified seeds of all species and unstratified seeds of most species was increased by light. Results showed no significant relationship between germination response to light and shade-tolerance ranking or mean seed weight of the species. In six seed lots of Pinus contorta var. latifolia, however, we detected a weak negative correlation between mean seed weight and unstratified light responsivity measured after 1 week but a significant positive correlation when measured after 3 weeks. Very low light levels in closed-canopy forests or in the forest floor may prolong tree seed germination but are unlikely to constrain final germination levels after most seeds have been naturally stratified by moist, cool winter conditions. The importance of differences in the rate and timing of tree seed germination under natural conditions remains to be demonstrated. Key words: conifer biology, forest regeneration, light response, lodgepole pine, Pinus contorta, seed germination, stratification.


Author(s):  
D. N. Pegler

Abstract A description is provided for Inonotus weirii. Information is included on the disease caused by the organism, its transmission, geographical distribution, and hosts. HOSTS: On Abies amabilis, A. grandis, A. lasiocarpa, Chamaecyparis spp., Larix occidentalis, Picea engelmannii, P. sitchensis, Pinus contorta, P. monticola, P. ponderosa, Pseudotsuga menziesii, Thuja plicata, Tsuga heterophylla. DISEASE: Laminated butt rot and yellow ring rot of conifers. GEOGRAPHICAL DISTRIBUTION: North America (Western Canada, Oregon, Washington); Japan. TRANSMISSION: The disease is spread by root contact with infected material in the soil; there appears to be little spread of the fungus by growth through the soil. It is likely that infection of freshly exposed wounds takes place through airborne basidiospores.


2000 ◽  
Vol 76 (2) ◽  
pp. 303-309 ◽  
Author(s):  
Christie Staudhammer ◽  
Valerie LeMay

Height equations for western hemlock (Tsuga heterophylla (Raf.) Sarg.), western red cedar (Thuja plicata Donn), Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco), and alder (Alnus rubra Bong. and Alnus tenuifolia Nutt.) were fitted using dbh as the predictor variable. A simple, non-linear equation gave very similar results to the Weibull distribution, except for hemlock, which was better modelled using the more flexible Weibull distribution function. Introducing stand density variables into the base equations resulted in increased accuracy for predicting heights of alder. Smaller improvements were found for Douglas-fir, cedar, and hemlock. Key words: estimating height, Coastal BC, Weibull estimation, stand density measures


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