Purification and characterization of an extracellular chitinase from the entomopathogen Metarhizium anisopliae
1997 ◽
Vol 43
(4)
◽
pp. 322-327
◽
Keyword(s):
Chitinases are produced by Metarhizium anisopliae when it is grown in the presence of chitin. A chitinase from the culture filtrate of Metarhizium anisopliae was successively purified by precipitation with ammonium sulphate, followed by anion-exchange chromatography on DEAE-Sephacel. The purified enzyme, which has a molecular mass of approximately 30 kDa by sodium dodecyl sulphate – polyacrylamide gel electrophoresis, catalyses the hydrolysis of p-nitrophenyl β-N-diacetylchitobiose with an apparent Km of 0.537 mmol and Vmax of 4.86 nmol∙mL−1∙min−1. The optimum pH and temperature were 4.5–5.0 and 40–45 °C, respectively.Key words: chitinases, Metarhizium anisopliae, enzyme purification, enzyme characterization.
2004 ◽
Vol 70
(4)
◽
pp. 2367-2372
◽
2017 ◽
Vol 9
(10)
◽
pp. 165
2000 ◽
Vol 66
(1)
◽
pp. 252-256
◽
1999 ◽
Vol 181
(1)
◽
pp. 91-99
◽
1985 ◽
Vol 31
(2)
◽
pp. 149-153
◽
2006 ◽
Vol 188
(3)
◽
pp. 902-908
◽
2002 ◽
Vol 184
(7)
◽
pp. 1932-1939
◽