THE IN VITRO UTILIZATION AND PRODUCTION OF NON-ESTERIFIED FATTY ACIDS BY ADRENALECTOMIZED RATS

1961 ◽  
Vol 39 (6) ◽  
pp. 1061-1065 ◽  
Author(s):  
W. F. Perry ◽  
Helen F. Bowen
Keyword(s):  
Carbon Dioxide ◽  
Fatty Acids ◽  
Adipose Tissue ◽  
Liver Tissue ◽  
In Vitro Production ◽  
Esterified Fatty Acids ◽  
Adrenalectomized Animal ◽  
Vitro Production ◽  
Adrenalectomized Rats

The in vitro utilization of non-esterified fatty acids by various tissues and the in vitro production of non-esterified fatty acids by adipose tissue have been compared in normal and adrenalectomized rats. It was found that the production of NEFA by adipose tissue was similar in both groups of animals but that the in vitro utilization of NEFA and production of carbon dioxide by heart, diaphragm, kidney, and liver tissue was greater in the adrenalectomized animal. These findings together with the depletion of fat content of the depots are interpreted as indicating that in the adrenalectomized state there is increased peripheral utilization of fatty acids.

ACETATE UTILIZATION BY LIVER AND ADIPOSE TISSUE OF RATS FASTED IN THE COLD

1958 ◽  
Vol 36 (1) ◽  
pp. 237-241
Author(s):  
William F. Perry
Keyword(s):  
Carbon Dioxide ◽  
Fatty Acids ◽  
Adipose Tissue ◽  
Fatty Acid ◽  
Liver Tissue ◽  
Carbon Dioxide Production ◽  
Acetate Incorporation ◽  
Fasted Rats ◽  
Glucose Supplementation

The in vitro incorporation of 1-C14 and 2-C14 acetate into fatty acids and carbon dioxide by liver and adipose tissue was studied in rats fasted at 5 °C. for 24 hours. Compared with fed rats at room temperature, there was a marked decrease in the incorporation of the acetate carbons into fatty acids and carbon dioxide by liver tissue. A pronounced decrease in acetate incorporation into fatty acid was also noted with adipose tissue from these same animals, but only a slight decrease in incorporation into carbon dioxide. Addition of glucose to the incubation medium caused increases in fatty acid formation by liver and adipose tissue from both normal and fasted animals, but glucose supplementation, while increasing the incorporation of acetate into carbon dioxide by liver tissue from cold fasted rats, did not affect carbon dioxide production by liver tissue from normal animals. Incorporation of acetate into carbon dioxide by adipose tissue was unaffected by glucose supplementation with tissue from both normal and cold fasted rats.

FACTORS AFFECTING THE IN VITRO PRODUCTION OF NON-ESTERIFIED FATTY ACID FROM ADIPOSE TISSUE

1962 ◽  
Vol 40 (6) ◽  
pp. 749-755 ◽  
Author(s):  
W. F. Perry ◽  
H. F. Bowen
Keyword(s):  
Growth Hormone ◽  
Adipose Tissue ◽  
Maximal Concentration ◽  
In Vitro Production ◽  
Factors Affecting ◽  
Esterified Fatty Acids ◽  
Rat Adipose Tissue ◽  
Vitro Production ◽  
Fasted Rats

The incubation of rat adipose tissue (epidymal fat) in the presence of growth hormone, ACTH, or epinephrine led to an increased production of non-esterified fatty acids (NEFA). The increased NEFA production induced by maximal concentration of the hormones was inhibited if insulin was also present in the medium but unaffected by the addition of glucose at a concentration of 300 mg%. Lower concentrations of growth hormone, however, were counteracted by this concentration of glucose.Adipose tissue from 72-hour fasted rats also exhibited increased NEFA production. Unlike the hormonally induced NEFA increase, the increase NEFA production by tissue from fasted animals was unaffected by insulin in the medium but was inhibited by the addition of glucose.

ACETATE UTILIZATION BY LIVER AND ADIPOSE TISSUE OF RATS FASTED IN THE COLD

1958 ◽  
Vol 36 (2) ◽  
pp. 237-241 ◽  
Author(s):  
William F. Perry
Keyword(s):  
Carbon Dioxide ◽  
Fatty Acids ◽  
Adipose Tissue ◽  
Fatty Acid ◽  
Liver Tissue ◽  
Carbon Dioxide Production ◽  
Acetate Incorporation ◽  
Fasted Rats ◽  
Glucose Supplementation

The in vitro incorporation of 1-C14 and 2-C14 acetate into fatty acids and carbon dioxide by liver and adipose tissue was studied in rats fasted at 5 °C. for 24 hours. Compared with fed rats at room temperature, there was a marked decrease in the incorporation of the acetate carbons into fatty acids and carbon dioxide by liver tissue. A pronounced decrease in acetate incorporation into fatty acid was also noted with adipose tissue from these same animals, but only a slight decrease in incorporation into carbon dioxide. Addition of glucose to the incubation medium caused increases in fatty acid formation by liver and adipose tissue from both normal and fasted animals, but glucose supplementation, while increasing the incorporation of acetate into carbon dioxide by liver tissue from cold fasted rats, did not affect carbon dioxide production by liver tissue from normal animals. Incorporation of acetate into carbon dioxide by adipose tissue was unaffected by glucose supplementation with tissue from both normal and cold fasted rats.

FACTORS AFFECTING THE IN VITRO PRODUCTION OF NON-ESTERIFIED FATTY ACID FROM ADIPOSE TISSUE

1962 ◽  
Vol 40 (1) ◽  
pp. 749-755 ◽  
Author(s):  
W. F. Perry ◽  
H. F. Bowen
Keyword(s):  
Growth Hormone ◽  
Adipose Tissue ◽  
Maximal Concentration ◽  
In Vitro Production ◽  
Factors Affecting ◽  
Esterified Fatty Acids ◽  
Rat Adipose Tissue ◽  
Vitro Production ◽  
Fasted Rats

The incubation of rat adipose tissue (epidymal fat) in the presence of growth hormone, ACTH, or epinephrine led to an increased production of non-esterified fatty acids (NEFA). The increased NEFA production induced by maximal concentration of the hormones was inhibited if insulin was also present in the medium but unaffected by the addition of glucose at a concentration of 300 mg%. Lower concentrations of growth hormone, however, were counteracted by this concentration of glucose.Adipose tissue from 72-hour fasted rats also exhibited increased NEFA production. Unlike the hormonally induced NEFA increase, the increase NEFA production by tissue from fasted animals was unaffected by insulin in the medium but was inhibited by the addition of glucose.

THE EFFECT OF VARIOUS SACCHARIDES ON THE RELEASE OF NON ESTERIFIED FATTY ACIDS FROM ADIPOSE TISSUE IN VITRO

1962 ◽  
Vol 40 (4) ◽  
pp. 455-458 ◽  
Author(s):  
W. F. Perry ◽  
R. J. Tjaden
Keyword(s):  
Fatty Acids ◽  
Adipose Tissue ◽  
Incubation Medium ◽  
Epididymal Adipose Tissue ◽  
Tissue Cell ◽  
Glycerol Phosphate ◽  
Esterified Fatty Acids ◽  
Adipose Tissue Cell ◽  
Other Substances

Rat epididymal adipose tissue was incubated in a phosphate–albumin medium to ascertain the effect of various saccharides and other substances on the release of non-esterified fatty acids (NEFA) into the medium. It was found that incubation with glucose, mannose, fructose, and 2-deoxy glucose resulted in less release of NEFA from the tissue into the incubation medium. Incubation with galactose, sucrose, lactose, D-ribose, D-xylose, L-xylose, D-arabinose, L-arabinose, D-lyxose, sodium pyruvate, glycerol, and glycerol phosphate showed no differences from the control in release of NEFA into the incubation medium. These results are consistent with the theory that the NEFA-lowering action of glucose is due to esterification of free fatty acid within the adipose tissue cell by glycerol phosphate.

Development of a simple, portable carbon dioxide incubator for in vitro production of bovine embryos

1999 ◽  
Vol 54 (3) ◽  
pp. 149-157 ◽  
Author(s):  
T Suzuki ◽  
C Sumantri ◽  
N.H.A Khan ◽  
M Murakami ◽  
S Saha
Keyword(s):  
Carbon Dioxide ◽  
Bovine Embryos ◽  
In Vitro Production ◽  
Vitro Production

THE UTILIZATION OF ACETATE BY LIVER AND ADIPOSE TISSUE OF GROWTH HORMONE TREATED RATS OF DIFFERENT AGES

1957 ◽  
Vol 35 (1) ◽  
pp. 759-766 ◽  
Author(s):  
W. F. Perry ◽  
Helen F. Bowen
Keyword(s):  
Carbon Dioxide ◽  
Fatty Acids ◽  
Growth Hormone ◽  
Adipose Tissue ◽  
Young Adult ◽  
Age Groups ◽  
Fat Content ◽  
Liver Slices ◽  
Old Rats

The effect of growth hormone on the in vitro incorporation of C14 acetate into fatty acids, carbon dioxide, and cholesterol by liver and adipose tissue from young, adult, and old rats was studied.In all three age groups of animals, growth hormone was found to depress the incorporation of acetate into fatty acids by liver slices but the CO2 production was unaffected. In both young and old animals growth hormone did not significantly alter the incorporation of acetate into fatty acids and CO2 by preparations of adipose tissue, but did result in a decline in the fat content of the adipose tissue. It was noted that the CO2 production from acetate was much less with adipose tissue from old rats than with similar preparations from young rats.Incorporation of acetate into cholesterol was unaffected by growth hormone in young and old animals but was significantly increased in liver slices from adult animals.

Sign in / Sign up

Export Citation Format

Share Document