ALDOLASE ACTIVITY IN NORMAL AND DYSTROPHIC MOUSE MUSCLE

1964 ◽  
Vol 42 (9) ◽  
pp. 1301-1305 ◽  
Author(s):  
U. Srivastava ◽  
L. Berlinguet
Keyword(s):  
Nitrogen Content ◽  
Total Nitrogen ◽  
Total Nitrogen Content ◽  
Dystrophic Muscle ◽  
Mouse Muscle ◽  
Dystrophic Mouse ◽  
Aldolase Activity ◽  
Dystrophic Mice

Aldolase activity and nitrogen content of the muscle were determined in hereditary muscular dystrophic mice and their normal litter mates at various ages. Aldolase activity was found to decrease in dystrophic muscle when expressed per mg of wet tissue but showed an increase at later stages of the disease when expressed per mg of total nitrogen in muscle. Total nitrogen content of dystrophic muscle decreased considerably during the evolution of the disease. In normal mice, the muscle aldolase activity increases with age.

PROTEOLYTIC ENZYMES IN NORMAL AND DYSTROPHIC MOUSE MUSCLE

1966 ◽  
Vol 44 (5) ◽  
pp. 613-623 ◽  
Author(s):  
L. Berlinguet ◽  
U. Srivastava
Keyword(s):  
Proteolytic Enzymes ◽  
Chemical Change ◽  
Optimum Conditions ◽  
Normal Muscle ◽  
Dystrophic Muscle ◽  
Muscle Enzymes ◽  
Mouse Muscle ◽  
Ph Values ◽  
Dystrophic Mouse ◽  
Dystrophic Mice

Proteolytic enzymes extracted from normal and dystrophic mouse muscle were studied, and optimum conditions for their activities were established. It was found that these enzymes were active at two pH values, 7.5 and 9. In normal and dystrophic mice, the enzymatic activity increased with age. When the activities of dystrophic muscle enzymes were compared with those of normal muscle enzymes, the increase was most significant in animals 60–90 days of age. The results obtained when the enzymes extracted from normal or dystrophic muscle were incubated with substrates from normal or dystrophic muscle indicate that the defect in the muscle is due to an increase in the activities of the proteolytic enzymes rather than to a chemical change in the muscle proteins.

CHOLINESTERASE AND MONOAMINE OXIDASE ACTIVITIES IN SKELETAL MUSCLE OF NORMAL AND HEREDITARY DYSTROPHIC MICE

1967 ◽  
Vol 45 (4) ◽  
pp. 573-580 ◽  
Author(s):  
Uma Srivastava ◽  
Louis Berlinguet
Keyword(s):  
Monoamine Oxidase ◽  
Total Nitrogen ◽  
Oxidase Activity ◽  
High Sensitivity ◽  
Lens Protein ◽  
Fresh Weight ◽  
Dystrophic Muscle ◽  
Mouse Muscle ◽  
Dystrophic Mouse ◽  
Amine Content

Acetyl- and butyryl-cholinesterase activities in dystrophic mouse muscle are increased significantly, whether the results are expressed in relation to (a) the fresh weight or (b) the total nitrogen, of the muscle. Activities of these enzymes do not show any change in normal and dystrophic mouse liver and brain. However, in dystrophic lens, the enzymatic activity is decreased when the results are expressed on the basis of fresh weight. This is because of a change in the chemical composition of the lens since no significant change in both enzymatic activities could be observed when the results were expressed on the basis of lens protein. This increase in the cholinesterase activity of dystrophic muscle is not incompatible with the change in the distribution pattern of these enzymes in the muscle noted by many workers, or to the high sensitivity of dystrophic muscle to acetylcholine.Monoamine oxidase activity in dystrophic mouse muscle showed a significant increase when the results are expressed in relation to (a) the fresh weight or (b) the total nitrogen, of the muscle. This increase in activity could be due to enzyme induction, i.e. increase in the amine content followed by an increase in monoamine oxidase activity in dystrophic muscle.

Operational Results of the Wolfsburg Wastewater Treatment Plant

1992 ◽  
Vol 25 (4-5) ◽  
pp. 203-209 ◽  
Author(s):  
R. Kayser ◽  
G. Stobbe ◽  
M. Werner
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Nitrogen Content ◽  
Total Nitrogen ◽  
Nitrogen Removal ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Phosphate Removal ◽  
Total Nitrogen Content ◽  
Activated Sludge Process

At Wolfsburg for a load of 100,000 p.e., the step-feed activated sludge process for nitrogen removal is successfully in operation. Due to the high denitrification potential (BOD:TKN = 5:1) the effluent total nitrogen content can be kept below 10 mg l−1 N; furthermore by some enhanced biological phosphate removal about 80% phosphorus may be removed without any chemicals.

Enzyme studies of skeletal muscle in mice with hereditary muscular dystrophy

1963 ◽  
Vol 205 (5) ◽  
pp. 897-901 ◽  
Author(s):  
Marilyn W. McCaman
Keyword(s):  
Skeletal Muscle ◽  
Muscular Dystrophy ◽  
Glutathione Reductase ◽  
Enzyme Activities ◽  
Lactic Dehydrogenase ◽  
Normal Muscle ◽  
Dystrophic Muscle ◽  
Nerve Section ◽  
Mouse Muscle ◽  
Dystrophic Mouse

The activities of 20 enzymes in normal, heterozygous, and dystrophic mouse muscle were studied by means of quantitative microchemical methods. Enzyme activities in normal and heterozygous muscle were essentially the same. In dystrophic muscle glucose-6-P dehydrogenase, 6-P-gluconic dehydrogenase, glutathione reductase, peptidase, ß-glucuronidase, and glucokinase activities were significantly higher than in normal muscle, while α-glycero-P dehydrogenase and lactic dehydrogenase activities were significantly lower. The pattern of enzyme activities found in normal gastrocnemius denervated by nerve section was strikingly similar to that in dystrophic muscle.

scholarly journals Exploring the role of macroalgal traits on the feeding behaviour of a generalist herbivore in Malaysian waters

2020 ◽  
Vol 63 (5) ◽  
pp. 407-417
Author(s):  
Lim Wai Yin ◽  
Lim Phaik Eem ◽  
Affendi Yang Amri ◽  
Song Sze Looi ◽  
Acga Cheng
Keyword(s):  
South China Sea ◽  
Nitrogen Content ◽  
Total Nitrogen ◽  
South China ◽  
Nutritional Quality ◽  
Feeding Behaviour ◽  
Total Nitrogen Content ◽  
Generalist Herbivore ◽  
Macroalgal Species ◽  
Straits Of Malacca

AbstractWith the potential adverse effects of climate change, it is essential to enhance the understanding of marine ecosystem dynamics, which can be driven by the co-evolutionary interaction between autotrophs and herbivores. This study looked into the autotroph-herbivore interactions in Malaysian waters, mainly to determine if autotroph nutritional quality significantly influences herbivore consumption rates. We documented the relative consumption rate of a generalist herbivore (Chanos chanos Forsskål) obtained from the Straits of Malacca through multiple feeding trials using 12 macroalgal species collected from different coastal areas of the Straits of Malacca, the Straits of Johor, and the South China Sea. The herbivore fed selectively on the tested macroalgal species, with the most and least consumed species having the lowest and highest total nitrogen content, respectively. Besides total nitrogen content, the least consumed species also had the highest total phenolic content. Interestingly, we observed that the herbivore generally preferred to consume filamentous macroalgae, especially those collected from the South China Sea. Overall, our findings demonstrated that the feeding behaviour of a generalist herbivore could be influenced by the nutritional quality of the autotrophs, which may depend directly or indirectly on other factors such as autotroph morphology and geography.

The Feasibility Research for Detecting the Nitrogen Content in Sewage with NIPGA Method

2014 ◽  
Vol 602-605 ◽  
pp. 2445-2448
Author(s):  
Fu Quan Jia ◽  
Zhu Jun Tian
Keyword(s):  
Nitrogen Content ◽  
Neutron Source ◽  
Total Nitrogen ◽  
Gamma Rays ◽  
Neutron Generator ◽  
Limit Of Detection ◽  
Total Nitrogen Content ◽  
Feasibility Research ◽  
Type Water

NIPGA technology is used in order to detect the total nitrogen content in sewage quickly. D-D neutron generator is used as the neutron source and BGO detector is used to detect gamma rays of nitrogen. The simulated result of MCNP shows the nitrogen’s limit of detection is 0.2 mg/L and the total nitrogen in V-type water can be detected. So this method can be used to detect the total nitrogen content in sewage quickly.

scholarly journals Co-Digestion of Vinasse Waste and Tofu Liquid Waste to Increase Biogas Production

Eksergi ◽  
2015 ◽  
Vol 12 (2) ◽  
pp. 23
Author(s):  
Iqbal Syaichurrozi
Keyword(s):  
Nitrogen Content ◽  
Total Nitrogen ◽  
Biogas Production ◽  
Liquid Waste ◽  
Volume Ratio ◽  
Total Nitrogen Content ◽  
Anaerobic Digesters ◽  
Initial Ph

The purpose of this study was to increase biogas production using co-digestion concept. Vinasse Waste (VW) containing high COD and low total Nitrogen content was mixtured with Tofu Liquid Waste (TLW) containing low COD and high total Nitrogen. Substrates were varied with volume ratio of VW:TLW of 100:0, 20:80, 0:100. Total volume of substrates was 250 mL. Anaerobic digesters were operated at room temperatur. After fermentation, biogas total volume of variables of 100:0, 20:80, 0:100 was 88.5; 125.5; 41.5 mL. Initial pH for all variables was 7.0. At the end of fermentation, pH substrates became 3.9; 5.1; 6.8 for variables of 100:0, 20:80, 0:100 respectively.

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