Rapid quantitative separation of long-chain fatty acids from neutral lipids

1969 ◽  
Vol 47 (7) ◽  
pp. 746-750 ◽  
Author(s):  
Jean-Guy Durocher ◽  
Bernard Leboeuf
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Methyl Esters ◽  
Neutral Lipids ◽  
Methyl Esters ◽  
Long Chain Fatty Acids ◽  
Neutral Alumina ◽  
Quantitative Separation ◽  
Hydrolysis Of ◽  
Long Chain ◽  
Chain Fatty Acids

On a microcolumn of neutral alumina, long-chain fatty acids are quantitatively retained. Neutral lipids including cholesteryl esters, triglycerides, diglycerides, and fatty acid methyl esters, as well as cholesterol, are quantitatively recovered in the effluent without hydrolysis of ester groups. Monoglyceride recovery is, however, only 96 ± 2%. Preparation of the column and chromatography may be accomplished in less than 45 min.

Very long-chain fatty acids in neutral lipids and glycerophospholipids of adrenoleukodystrophy-cultured skin fibroblasts

1985 ◽  
Vol 71 (2-3) ◽  
pp. 301-306 ◽  
Author(s):  
Tetsuo Sakai ◽  
Yasunobu Antoku ◽  
Ikuo Goto ◽  
Jun Ochiai ◽  
Hiroshi Iwashita ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Neutral Lipids ◽  
Skin Fibroblasts ◽  
Long Chain Fatty Acids ◽  
Cultured Skin ◽  
Long Chain ◽  
Chain Fatty Acids

scholarly journals Very long-chain fatty acids are accumulated in triacylglycerol and nonesterified forms in colorectal cancer tissues

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kotaro Hama ◽  
Yuko Fujiwara ◽  
Tamuro Hayama ◽  
Tsuyoshi Ozawa ◽  
Keijiro Nozawa ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Fatty Acids ◽  
Neutral Lipids ◽  
Long Chain Fatty Acids ◽  
Liquid Chromatography Mass Spectrometry ◽  
Nonesterified Fatty Acids ◽  
Precise Diagnosis ◽  
Cancer Tissues ◽  
Long Chain ◽  
Chain Fatty Acids

AbstractColorectal cancer (CRC) is a major cancer, and its precise diagnosis is especially important for the development of effective therapeutics. In a series of metabolome analyses, the levels of very long chain fatty acids (VLCFA) were shown to be elevated in CRC tissues, although the endogenous form of VLCFA has not been fully elucidated. In this study we analyzed the amount of nonesterified fatty acids, acyl-CoA species, phospholipids and neutral lipids such as cholesterylesters using liquid-chromatography–mass spectrometry. Here we showed that VLCFA were accumulated in triacylglycerol (TAG) and nonesterified forms in CRC tissues. The levels of TAG species harboring a VLCFA moiety (VLCFA-TAG) were significantly correlated with that of nonesterified VLCFA. We also showed that the expression level of elongation of very long-chain fatty acids protein 1 (ELOVL1) is increased in CRC tissues, and the inhibition of ELOVL1 decreased the levels of VLCFA-TAG and nonesterified VLCFA in CRC cell lines. Our results suggest that the upregulation of ELOVL1 contributes to the accumulation of VLCFA-TAG and nonesterified VLCFA in CRC tissues.

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