scholarly journals Very long-chain fatty acids are accumulated in triacylglycerol and nonesterified forms in colorectal cancer tissues

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kotaro Hama ◽  
Yuko Fujiwara ◽  
Tamuro Hayama ◽  
Tsuyoshi Ozawa ◽  
Keijiro Nozawa ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Fatty Acids ◽  
Neutral Lipids ◽  
Long Chain Fatty Acids ◽  
Liquid Chromatography Mass Spectrometry ◽  
Nonesterified Fatty Acids ◽  
Precise Diagnosis ◽  
Cancer Tissues ◽  
Long Chain ◽  
Chain Fatty Acids

AbstractColorectal cancer (CRC) is a major cancer, and its precise diagnosis is especially important for the development of effective therapeutics. In a series of metabolome analyses, the levels of very long chain fatty acids (VLCFA) were shown to be elevated in CRC tissues, although the endogenous form of VLCFA has not been fully elucidated. In this study we analyzed the amount of nonesterified fatty acids, acyl-CoA species, phospholipids and neutral lipids such as cholesterylesters using liquid-chromatography–mass spectrometry. Here we showed that VLCFA were accumulated in triacylglycerol (TAG) and nonesterified forms in CRC tissues. The levels of TAG species harboring a VLCFA moiety (VLCFA-TAG) were significantly correlated with that of nonesterified VLCFA. We also showed that the expression level of elongation of very long-chain fatty acids protein 1 (ELOVL1) is increased in CRC tissues, and the inhibition of ELOVL1 decreased the levels of VLCFA-TAG and nonesterified VLCFA in CRC cell lines. Our results suggest that the upregulation of ELOVL1 contributes to the accumulation of VLCFA-TAG and nonesterified VLCFA in CRC tissues.

scholarly journals Non-esterified Very Long-chain Fatty Acids Are Accumulated in Colorectal Cancer Tissues

2020 ◽  
Author(s):  
Kotaro Hama ◽  
Yuko Fujiwara ◽  
Tamuro Hayama ◽  
Tsuyoshi Ozawa ◽  
Keijiro Nozawa ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Fatty Acids ◽  
Cultured Cells ◽  
Fatty Acid Transport ◽  
Long Chain Fatty Acids ◽  
Liquid Chromatography Mass Spectrometry ◽  
Precise Diagnosis ◽  
Cancer Tissues ◽  
Long Chain ◽  
Chain Fatty Acids

Abstract Colorectal cancer (CRC) is a major cancer, and its precise diagnosis is especially important for the development of effective therapeutics. In a series of metabolome analyses, the levels of very long chain fatty acids (VLCFA) was shown to be elevated in CRC tissues, although the endogenous form of VLCFA has not been fully elucidated. In this study we analyzed the amount of non-esterified fatty acids, phospholipids and acyl-CoA species by liquid-chromatography–mass spectrometry and showed that VLCFA is accumulated as the non-esterified form in CRC tissues. We also showed that the expression level of elongation of very long-chain fatty acids 1 (ELOVL1) is increased, whereas fatty acid transport protein 4 (FATP4) is decreased in CRC tissues. Finally, we showed that the amount of non-esterified VLCFA species was significantly up-regulated in cultured cells overexpressing ELOVL1. Our results suggest that the upregulation of ELOVL1 and the down-regulation of FATP4 cooperatively lead to the accumulation of non-esterified VLCFA in CRC tissues.

Very long-chain fatty acids in neutral lipids and glycerophospholipids of adrenoleukodystrophy-cultured skin fibroblasts

1985 ◽  
Vol 71 (2-3) ◽  
pp. 301-306 ◽  
Author(s):  
Tetsuo Sakai ◽  
Yasunobu Antoku ◽  
Ikuo Goto ◽  
Jun Ochiai ◽  
Hiroshi Iwashita ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Neutral Lipids ◽  
Skin Fibroblasts ◽  
Long Chain Fatty Acids ◽  
Cultured Skin ◽  
Long Chain ◽  
Chain Fatty Acids

scholarly journals Evaluating Ultra-long-Chain Fatty Acids as Biomarkers of Colorectal Cancer Risk

2016 ◽  
Vol 25 (8) ◽  
pp. 1216-1223 ◽  
Author(s):  
Kelsi Perttula ◽  
William M.B. Edmands ◽  
Hasmik Grigoryan ◽  
Xiaoming Cai ◽  
Anthony T. Iavarone ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Fatty Acids ◽  
Cancer Risk ◽  
Colorectal Cancer Risk ◽  
Long Chain Fatty Acids ◽  
Long Chain ◽  
Chain Fatty Acids

scholarly journals Structure and lipid distribution of polyenoic very-long-chain fatty acids in the brain of peroxisome-deficient patients (Zellweger syndrome)

1987 ◽  
Vol 248 (1) ◽  
pp. 61-67 ◽  
Author(s):  
P Sharp ◽  
A Poulos ◽  
A Fellenberg ◽  
D Johnson
Keyword(s):  
Fatty Acids ◽  
Neutral Lipids ◽  
Zellweger Syndrome ◽  
Carbon Chain ◽  
Chain Elongation ◽  
Normal Brain ◽  
Long Chain Fatty Acids ◽  
Beta Oxidation ◽  
Long Chain ◽  
Chain Fatty Acids

The polyenoic fatty acids with carbon chain lengths from 26 to 38 (very-long-chain fatty acids, VLCFA) previously detected in abnormal amounts in Zellweger syndrome brain have been shown to be n-6 derivatives and therefore probably derived by chain elongation of shorter-chain n-6 fatty acids such as linoleic acid and arachidonic acid. Polyenoic VLCFA are also present in Zellweger syndrome liver, but this tissue differs significantly from brain in that the saturated and mono-unsaturated derivatives are the major VLCFA. Zellweger syndrome brain polyenoic VLCFA are present in the neutral lipids predominantly in cholesterol esters, with smaller amounts in the non-esterified fatty acid and triacylglycerol fractions. These fatty acids are barely detectable in any of the major phospholipids, but are present in significant amounts in an unidentified minor phospholipid. The polyenoic VLCFA composition of this lipid differs markedly from that observed for all other lipids, as it contains high proportions of pentaenoic and hexaenoic fatty acids with 34, 36 and 38 carbon atoms. A polar lipid with the chromatographic properties in normal brain contains similar fatty acids. It is postulated that the polyenoic VLCFA may play an important role in normal brain and accumulate in Zellweger syndrome brain because of a deficiency in the peroxisomal beta-oxidation pathway, although a possible peroxisomal role in the control of carbon-chain elongation cannot be discounted.

Rapid quantitative separation of long-chain fatty acids from neutral lipids

1969 ◽  
Vol 47 (7) ◽  
pp. 746-750 ◽  
Author(s):  
Jean-Guy Durocher ◽  
Bernard Leboeuf
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Methyl Esters ◽  
Neutral Lipids ◽  
Methyl Esters ◽  
Long Chain Fatty Acids ◽  
Neutral Alumina ◽  
Quantitative Separation ◽  
Hydrolysis Of ◽  
Long Chain ◽  
Chain Fatty Acids

On a microcolumn of neutral alumina, long-chain fatty acids are quantitatively retained. Neutral lipids including cholesteryl esters, triglycerides, diglycerides, and fatty acid methyl esters, as well as cholesterol, are quantitatively recovered in the effluent without hydrolysis of ester groups. Monoglyceride recovery is, however, only 96 ± 2%. Preparation of the column and chromatography may be accomplished in less than 45 min.

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