Comparison of Liver Glycogen Synthetase between Streptozotocin-Diabetic and Control Rats

1972 ◽  
Vol 50 (6) ◽  
pp. 714-717 ◽  
Author(s):  
Albert Y. Chang

Chronic diabetic rat, induced by streptozotocin, showed high blood glucose but low liver glycogen levels. The diabetic liver had subnormal amounts of glycogen synthetase and its I form when assayed in both 8000 × g supernatant and glycogen pellet suspensions. The pellet suspension of the control liver retained 68% synthetase I activity; the diabetic liver retained only 19.5%. The addition of deproteinized liver extract to the pellet suspension increased the activity of synthetase either in the presence or absence of glucose-6-PO4 for both groups of animals to different degrees. The deproteinized liver extract of the diabetic rat contained little ATP, less glucose-6-PO4, and slightly more inorganic phosphate than that of the control rat. The significance of these observations is discussed.


1970 ◽  
Vol 39 (3) ◽  
pp. 375-382 ◽  
Author(s):  
G. A. O. Alleyne ◽  
H. S. Fraser ◽  
H. S. Besterman

1. Metabolic acidosis was induced by feeding ammonium chloride to rats which were maintained on a carbohydrate diet for 48 h. 2. Fasting blood glucose was the same in acidotic and control animals, but there was an increase in liver glycogen in the former. Muscle glycogen was unchanged. 3. In vitro glycogenolysis was the same in liver slices from normal rats when incubated at a range of pH from 6·90 to 7·40. 4. The peak blood glucose in response to intraperitoneal injections of glucagon was the same in control and acidotic rats. The rate of disappearance of glucose was slower in acidotic rats both after the glucagon induced hyperglycaemia and after intravenously injected glucose. 5. Liver phosphorylase, total glycogen synthetase and the I form of this enzyme were unchanged in acidosis. 6. The data are compatible with the hypothesis that in the acidotic rat there is a block in glycolysis—possibly at the phosphofructokinase step.



Author(s):  
Fadwa El-Ouady ◽  
Nadia Lahrach ◽  
Mohammed Ajebli ◽  
Ahmed E. Haidani ◽  
Mohamed Eddouks

Background: Diabetes mellitus is associated with high blood glucose levels due to insulin shortcoming (insulinopenia) or defective insulin action. The objective of the study was to investigate the antidiabetic and antioxidant effects of Foeniculum vulgare in streptozotocin-induced diabetic rat. Methods: The effects of the leaves aqueous extract (LAE) of Foeniculum vulgare (F. vulgare) at a dose of 10 mg/kg on blood glucose levels were evaluated in normal and streptozotocin (STZ)- induced diabetic rats. Histopathological changes were also evaluated in liver in STZ-induced rats. Results: Single oral administration of F. vulgare LAE reduced blood glucose levels 6 h after administration in STZ diabetic rats (p<0.0001). Furthermore, blood glucose levels were decreased in both normal (p<0.05) and STZ diabetic rats (p<0.0001) after the fifteenth day of treatment. During this test, both groups did not show any significant change in their body weight. Moreover, this aqueous extract improved oral glucose tolerance in diabetic rats and revealed a positive effect on liver histology. On the other hand, the extract used in this experiment showed an inhibitory concentration (IC50) of 50% of free radicals with a concentration of 43±1.19 µg/ml. While the synthetic antioxidant (BHT) had an IC50 equal to 22.67±2.17µg /ml. Conclusion: This study demonstrates the antihyperglycemic, hypoglycemic and antioxidant effects of the leaves of F. vulgare in normal and diabetic rats.





1960 ◽  
Vol 38 (1) ◽  
pp. 1471-1478 ◽  
Author(s):  
Dorothy S. Dow ◽  
C. E. Allen

The single intraperitoneal injection of a labelled glucose load has been shown to maintain the specific activities of blood glucose and expired CO2 at constant levels for extended periods of time. These plateaus have been established in both the fed control and the fed diabetic rat. The results have been interpreted as indicating the existence of a steady state in the labelled system under study.Measurements have been made of rates of oxidation of glucose-1-C14 and giucose-6-C14 in diabetic and control rats during this steady state between the specific activities of blood glucose and expired CO2. The results indicate no reduction either for the glycolytic or for the phosphogluconate pathway in the diabetic. No change in the ratio between the oxidative rates for the two pathways has been found.



2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Afrin Iqbal ◽  
Karar Zunaid Ahsan ◽  
Kanta Jamil ◽  
M. Moinuddin Haider ◽  
Shusmita Hossain Khan ◽  
...  

Abstract Background Bangladesh is well advanced in the epidemiologic transition from communicable to noncommunicable diseases, which now account for two out of three deaths annually. This paper examines the latest nationally representative hypertension prevalence estimates, awareness, treatment, and control—to identify their association with potential correlates. Methods The analyses are based on the recent Bangladesh Demographic and Health Survey 2017–18 data. Univariate analyses and bivariate analyses between the outcome variables and individual covariates were carried out. Then chi-square tests were done to see the proportional differences between them. To examine the demographic, socioeconomic and biological factors affecting hypertension, awareness, treatment and control, we used multivariate logistic regression models. Results We found that prevalence of hypertension for females and males together aged 35 or more has risen by half between 2011 (25.7%) to 2017 (39.4%). With the broader age range used in 2017, the prevalence is now 27.5% in the population aged 18 years or more. The factors associated with hypertension included older age, being female, urban residence, higher wealth status, minimal education, higher body mass index and high blood glucose level. Following multivariate analyses, many of these characteristics were no longer significant, leaving only age, being female, nutritional status and elevated blood glucose level as important determinants. Over half (58%) of females and males who were found to be hypertensive were not aware they had the condition. Only one in eight (13%) had the condition under control. Conclusion In the coming years, a rising trend in hypertension in Bangladeshi adults is expected due to demographic transition towards older age groups and increase in overweight and obesity among the population of Bangladesh. With more women being hypertensive than men, a targeted approach catering to high risk groups should be thoroughly implemented following the Multisectoral NCD Action Plan 2018–2025. Acting in close collaboration with other ministries/relevant sectors to bring an enabling environment for the citizens to adopt healthy lifestyle choices is a prerequisite for adequate prevention. While screening the adult population is essential, the public sector cannot possibly manage the ever-expanding numbers of hypertensives. The private sector and NGOs need to be drawn into the program to assist.



1952 ◽  
Vol 51 (1) ◽  
pp. 18-20 ◽  
Author(s):  
M. J. H. Smith ◽  
B. W. Meade ◽  
J. Bornstein


Author(s):  
Khalid Abbas Owish Sukar ◽  
Rihab Ibrahim Abdalla ◽  
Humeda Suekit Humeda ◽  
Ahmed Omer Alameen ◽  
Eltayeb Ibrahim Mubarak

Aims: This study was designed to investigate the effect of Pear millet on glycaemic control and lipid profile in streptozocin diabetic rat model. Methodology: Forty healthy mature male rats were used in this study. The rats divided into 4 groups, ten rats in each and group (A) and (B) normal control rats while group (C) and (D) considered as diabetic rats. Diabetes induced by intraperitoneal injection of 40 mg/kg streptozocin and confirmed by high blood glucose level which considered day 0. The experiment 1, included two groups (A and C), equal rats and the parameters investigated were measured in days 0, 14 and 28. The experiment 2 included two groups (B and D) were received 20% pearl millet and the blood samples were measured in days 0, 14 and 28. Results: The obtained results revealed significant (P<0.05) reduction in insulin and adiponectin (P<0.001) and elevation of blood glucose (P<0.001) in diabetic rats in group C, while significant (P<0.05) reductions in blood glucose, LDL levels and significant (P<0.05) elevation in adiponectin and HDL levels were detected in rats in group B and D.  Conclusions: The studies provide evidence that pearl millet induces hypoglycemic effect and improved lipidemic control in diabetic rats.



1976 ◽  
Vol 69 (1) ◽  
pp. 77-83 ◽  
Author(s):  
J. M. FÉLIX ◽  
R. L. JACQUOT

SUMMARY Sub-total pancreatectomy in utero was performed in 18-day-old rat foetuses. Pancreatectomized, sham-operated and control foetuses were collected 3 days later and body weight, glucose and insulin levels in blood, and glycogen content and glucose-6-phosphatase (G-6-Pase) activity of the liver were determined. Pancreatectomized foetuses showed only very small pancreatic remnants (≤ 1 mg) and accordingly their insulin levels were much lower (four to five times) than those of sham-operated or control foetuses; their blood glucose levels were slightly increased and liver glycogen content and G-6-Pase activity were slightly reduced; their body weights were also reduced. These results are discussed in relation to other relevant data in the literature. They afford direct experimental evidence of the endogenous origin of insulin in the foetal blood. It is suggested that during the last days of intra-uterine life insulin merely completes the action of the glucocorticoids on glycogen storage in rat foetal liver and probably contributes to foetal body growth. Its relative ineffectiveness on the foetal blood glucose level is not explained. As pancreatectomized foetuses develop sub-normal liver G-6-Pase activity, glucagon is probably not responsible for the increase in this activity occurring during normal development before birth.



1960 ◽  
Vol 38 (12) ◽  
pp. 1471-1478 ◽  
Author(s):  
Dorothy S. Dow ◽  
C. E. Allen

The single intraperitoneal injection of a labelled glucose load has been shown to maintain the specific activities of blood glucose and expired CO2 at constant levels for extended periods of time. These plateaus have been established in both the fed control and the fed diabetic rat. The results have been interpreted as indicating the existence of a steady state in the labelled system under study.Measurements have been made of rates of oxidation of glucose-1-C14 and giucose-6-C14 in diabetic and control rats during this steady state between the specific activities of blood glucose and expired CO2. The results indicate no reduction either for the glycolytic or for the phosphogluconate pathway in the diabetic. No change in the ratio between the oxidative rates for the two pathways has been found.



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