Basket cell development in the normal and hypothyroid rat cerebellar cortex studied with a monoclonal anti-neurofilament antibody

We have used a monoclonal antibody against an antigenic determinant of the 210-kdalton neurofilament protein to study basket cell maturation in rat cerebellar cortex. Neurofilament immunoreactivity first appears in basket cells at postnatal day 12 and mature axonal "pinceaux" are present at postnatal day 17. There are large differences in the rate of maturation from lobe to lobe which do not fully correspond to the rate of Purkinje cell differentiation. In hypothyroid rats the expression of the neurofilament antigen by basket cells is almost completely suppressed.

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Transient expression of neurofilament protein without filament formation in purkinje cell development

International Journal of Developmental Neuroscience ◽

10.1016/0736-5748(85)90071-1 ◽

1985 ◽

Vol 3 (4) ◽

pp. 365-377 ◽

Cited By ~ 18

Author(s):

Amico Bignami ◽

Milena Grossi ◽

Doris Dahl

Keyword(s):

Purkinje Cell ◽

Transient Expression ◽

Cell Development ◽

Filament Formation ◽

Neurofilament Protein

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Purkinje cell neurotransmission patterns cerebellar basket cells into zonal modules that are defined by distinct pinceau sizes

10.1101/2020.01.28.923896 ◽

2020 ◽

Cited By ~ 2

Author(s):

Joy Zhou ◽

Amanda M. Brown ◽

Elizabeth P. Lackey ◽

Marife Arancillo ◽

Tao Lin ◽

...

Keyword(s):

Purkinje Cell ◽

Purkinje Cells ◽

Initial Segment ◽

Molecular Diversity ◽

Basket Cell ◽

Basket Cells ◽

Conditional Allele ◽

Functional Zones ◽

Cell Somata ◽

Ramón Y Cajal

AbstractRamón y Cajal proclaimed the neuron doctrine based on circuit features he exemplified using cerebellar basket cell projections. Basket cells form dense inhibitory plexuses that wrap Purkinje cell somata and terminate as pinceaux at the initial segment of axons. Here, we demonstrate that HCN1, Kv1.1, PSD95 and GAD67 unexpectedly mark patterns of basket cell pinceaux that map onto Purkinje cell functional zones. Using cell-specific genetic tracing with an Ascl1CreERT2 mouse conditional allele, we reveal that basket cell zones comprise different sizes of pinceaux. We tested whether Purkinje cells instruct the assembly of inhibitory projections into zones, as they do for excitatory afferents. Genetically silencing Purkinje cell neurotransmission blocks the formation of sharp Purkinje cell zones and disrupts excitatory axon patterning. The distribution of pinceaux into size-specific zones is eliminated without Purkinje cell output. Our data uncover the cellular and molecular diversity of a foundational synapse that revolutionized neuroscience.

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[P1.50]: Atrazine delays Purkinje cell development in vitro as evidenced by Golgi method in primary organotypic cultures of mouse cerebellar cortex

International Journal of Developmental Neuroscience ◽

10.1016/j.ijdevneu.2008.09.100 ◽

2008 ◽

Vol 26 (8) ◽

pp. 858-858

Author(s):

Z. Peña‐Contreras ◽

D. Davila‐Vera ◽

R.V. Mendoza‐Briceño

Keyword(s):

Purkinje Cell ◽

Cerebellar Cortex ◽

Cell Development ◽

Golgi Method ◽

Organotypic Cultures ◽

Development In Vitro

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Purkinje cell neurotransmission patterns cerebellar basket cells into zonal modules defined by distinct pinceau sizes

eLife ◽

10.7554/elife.55569 ◽

2020 ◽

Vol 9 ◽

Author(s):

Joy Zhou ◽

Amanda M Brown ◽

Elizabeth P Lackey ◽

Marife Arancillo ◽

Tao Lin ◽

...

Keyword(s):

Purkinje Cell ◽

Purkinje Cells ◽

Initial Segment ◽

Molecular Diversity ◽

Basket Cell ◽

Basket Cells ◽

Conditional Allele ◽

Functional Zones ◽

Cell Somata ◽

Ramón Y Cajal

Ramón y Cajal proclaimed the neuron doctrine based on circuit features he exemplified using cerebellar basket cell projections. Basket cells form dense inhibitory plexuses that wrap Purkinje cell somata and terminate as pinceaux at the initial segment of axons. Here, we demonstrate that HCN1, Kv1.1, PSD95 and GAD67 unexpectedly mark patterns of basket cell pinceaux that map onto Purkinje cell functional zones. Using cell-specific genetic tracing with an Ascl1CreERT2 mouse conditional allele, we reveal that basket cell zones comprise different sizes of pinceaux. We tested whether Purkinje cells instruct the assembly of inhibitory projections into zones, as they do for excitatory afferents. Genetically silencing Purkinje cell neurotransmission blocks the formation of sharp Purkinje cell zones and disrupts excitatory axon patterning. The distribution of pinceaux into size-specific zones is eliminated without Purkinje cell GABAergic output. Our data uncover the cellular and molecular diversity of a foundational synapse that revolutionized neuroscience.

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