Regulation of transcription factor activity during cellular aging

Several lines of evidence suggest that the limited replication potential of normal human cells is due to the presence of an intrinsic genetic programme. This "senescence programme" is believed to reduce the incidence of cancer by limiting the growth of most of the transformed cells arising in vivo, although some cells do escape senescence becoming both immortalized and transformed. Here we review the literature that describes the senescence process in terms of gene expression and the regulation of gene expression by a variety of mechanisms affecting transcription factor activity. We focus on regulation of the c-fos gene through posttranslational modification of the serum response factor (SRF) as an example of altered gene expression during cellular aging.Key words: cellular aging, transcription, Fos, SRF, phosphorylation.

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The Sky's the LEMit: New insights into nuclear structure regulation of transcription factor activity

Current Opinion in Cell Biology ◽

10.1016/j.ceb.2020.10.006 ◽

2021 ◽

Vol 68 ◽

pp. 173-180

Author(s):

Amar N. Mirza ◽

Fernanda Gonzalez ◽

Sierra K. Ha ◽

Anthony E. Oro

Keyword(s):

Transcription Factor ◽

Nuclear Structure ◽

Regulation Of Transcription ◽

Transcription Factor Activity ◽

Factor Activity ◽

Structure Regulation

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Expresso: A database and web server for exploring the interaction of transcription factors and their target genes in Arabidopsis thaliana using ChIP-Seq peak data

F1000Research ◽

10.12688/f1000research.10041.1 ◽

2017 ◽

Vol 6 ◽

pp. 372 ◽

Cited By ~ 7

Author(s):

Delasa Aghamirzaie ◽

Karthik Raja Velmurugan ◽

Shuchi Wu ◽

Doaa Altarawy ◽

Lenwood S. Heath ◽

...

Keyword(s):

Gene Expression ◽

Transcription Factor ◽

Transcription Factors ◽

Chromatin Immunoprecipitation ◽

Target Genes ◽

Regulation Of Gene Expression ◽

Data Sets ◽

Motif Analysis ◽

Chromatin Immunoprecipitation Sequencing

Motivation: The increasing availability of chromatin immunoprecipitation sequencing (ChIP-Seq) data enables us to learn more about the action of transcription factors in the regulation of gene expression. Even though in vivo transcriptional regulation often involves the concerted action of more than one transcription factor, the format of each individual ChIP-Seq dataset usually represents the action of a single transcription factor. Therefore, a relational database in which available ChIP-Seq datasets are curated is essential. Results: We present Expresso (database and webserver) as a tool for the collection and integration of available Arabidopsis ChIP-Seq peak data, which in turn can be linked to a user’s gene expression data. Known target genes of transcription factors were identified by motif analysis of publicly available GEO ChIP-Seq data sets. Expresso currently provides three services: 1) Identification of target genes of a given transcription factor; 2) Identification of transcription factors that regulate a gene of interest; 3) Computation of correlation between the gene expression of transcription factors and their target genes. Availability: Expresso is freely available at http://bioinformatics.cs.vt.edu/expresso/

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REGULATION OF TRANSCRIPTION FACTOR ACTIVITY

Eukaryotic Transcription Factors ◽

10.1016/b978-012373983-4.50016-x ◽

2008 ◽

pp. 312-VI

Author(s):

David S. Latchman

Keyword(s):

Transcription Factor ◽

Regulation Of Transcription ◽

Transcription Factor Activity ◽

Factor Activity

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Transcription factor mTEAD-2 is selectively expressed at the beginning of zygotic gene expression in the mouse

Development ◽

10.1242/dev.124.10.1963 ◽

1997 ◽

Vol 124 (10) ◽

pp. 1963-1973 ◽

Cited By ~ 4

Author(s):

K.J. Kaneko ◽

E.B. Cullinan ◽

K.E. Latham ◽

M.L. DePamphilis

Keyword(s):

Gene Expression ◽

Transcription Factor ◽

Cdna Library ◽

Northern Analysis ◽

Transcription Factor Activity ◽

Factor Activity ◽

Rt Pcr ◽

Mouse Development ◽

Adult Tissues ◽

Zygotic Gene

mTEF-1 is the prototype of a family of mouse transcription factors that share the same TEA DNA binding domain (mTEAD genes) and are widely expressed in adult tissues. At least one member of this family is expressed at the beginning of mouse development, because mTEAD transcription factor activity was not detected in oocytes, but first appeared at the 2-cell stage in development, concomitant with the onset of zygotic gene expression. Since embryos survive until day 11 in the absence of mTEAD-1 (TEF-1), another family member likely accounts for this activity. Screening an EC cell cDNA library yielded mTEAD-1, 2 and 3 genes. RT-PCR detected RNA from all three of these genes in oocytes, but upon fertilization, mTEAD-1 and 3 mRNAs disappeared. mTEAD-2 mRNA, initially present at approx. 5,000 copies per egg, decreased to approx. 2,000 copies in 2-cell embryos before accumulating to approx. 100,000 copies in blastocysts, consistent with degradation of maternal mTEAD mRNAs followed by selective transcription of mTEAD-2 from the zygotic genome. In situ hybridization did not detect mTEAD RNA in oocytes, and only mTEAD-2 was detected in day-7 embryos. Northern analysis detected all three RNAs at varying levels in day-9 embryos and in various adult tissues. A fourth mTEAD gene, recently cloned from a myotube cDNA library, was not detected by RT-PCR in either oocytes or preimplantation embryos. Together, these results reveal that mTEAD-2 is selectively expressed for the first 7 days of embryonic development, and is therefore most likely responsible for the mTEAD transcription factor activity that appears upon zygotic gene activation.

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