Crude-oil-degrading thermophilic bacterium isolated from an oil field

2004 ◽  
Vol 50 (3) ◽  
pp. 175-182 ◽  
Author(s):  
Ruixia Hao ◽  
Anhuai Lu ◽  
Guanyu Wang
Keyword(s):  
Crude Oil ◽  
Volatile Fatty Acids ◽  
Chemical Properties ◽  
Culture Conditions ◽  
Thermophilic Bacterium ◽  
Oil Field ◽  
Crude Oils ◽  
Organic Substrates ◽  
16S Rrna Sequence ◽  
Ph Range

Thermophilic bacterium strain C2, which has the ability to transform crude oils, was isolated from the reservoir of the Shengli oil field in East China. The Gram-negative, rod-shaped, nonmotile cells were grown at a high temperature, up to 83 °C, in the neutral to alkaline pH range. Depending on the culture conditions, the organism occurred as single rods or as filamentous aggregates. Strain C2 was grown chemoorganotrophically and produced metabolites, such as volatile fatty acids, 1,2-benzenedicarboxylic acid, bis(2-ethylhexyl)ester, dibutyl phthalate, and di-n-octyl phthalate. It could metabolize different organic substrates (acetate, D-glucose, fructose, glycerol, maltose, pyruvate, starch, sucrose, xylose, hexadecane). The G+C content (68 mol%) and the 16S rRNA sequence of strain C2 indicated that the isolate belonged to the genus Thermus. The strain affected different crude oils and changed their physical and chemical properties. The biochemical interactions between crude oils and strain C2 follow distinct trends characterized by a group of chemical markers (saturates, aromatics, resins, asphaltenes). Those trends show an increase in saturates and a decrease in aromatics, resins, and asphaltenes. The bioconversion of crude oils leads to an enrichment in lighter hydrocarbons and an overall redistribution of these hydrocarbons.Key words: thermophile, metabolite, crude oil, degradation, conversion.

Laser-induced Fluorescence with Multivariate Analysis in Characterizing Crude Oils from Some Selected Oil Fields in Ghana

2021 ◽  
pp. 8-20
Author(s):  
Calvin Kwesi Gafrey ◽  
Robert Wilson ◽  
George Amoako ◽  
Benjamin Anderson
Keyword(s):  
Multivariate Analysis ◽  
Crude Oil ◽  
Fluorescence Spectra ◽  
Continuous Wave ◽  
Laser Induced Fluorescence ◽  
Oil Field ◽  
Oil Fields ◽  
Crude Oils ◽  
Scientific Practices ◽  
Linear Discriminant

Developing scientific practices and procedures for finding the characteristics of various crude oils from different geological sources based on fluorescence spectra fingerprints would be beneficial to the petroleum industry. Laser-Induced Fluorescence (LIF) has gained relevance worldwide because of its advantages in crude oil analysis. Presently, the use of this technique in the characterization of crude oils from the oil fields in Ghana has not been studied. The study employed the LIF technique to determine some physical qualities of crude oils from Jubilee Oil Field, Tweneboa Enyenra Ntomme (TEN) Oil Field and Saltpond Oil Field. Specifically, this study used multivariate analysis methods to link the spectral signatures of the crude oils to their properties for identification and classification. The LIF technique was applied on four crude oil samples. Fluorescence spectra were obtained using a continuous wave 405.0 nm laser. The excitation source revealed five (5) peak wavelengths after deconvolution. Using Principal Component Analysis (PCA), Linear Discriminant Analysis (LDA) and Hierarchical Cluster Analysis (HCA), the crude oil samples were classified accurately.

The Effect of EOR Technologies on the Content of Petroporphyrins and Naphthenic Acids of Recovered Heavy Oils from Usinskoye Field

2015 ◽  
Vol 670 ◽  
pp. 39-43 ◽  
Author(s):  
Larisa D. Stakhina ◽  
Danil S. Petrenko ◽  
Adina S. Spabekova
Keyword(s):  
Crude Oil ◽  
Heavy Oil ◽  
Naphthenic Acids ◽  
High Density ◽  
Oil Field ◽  
Steam Treatment ◽  
Crude Oils ◽  
Heavy Oils ◽  
Oil Displacement

The effect of EOR technologies on the contents of petroporphyrins and naphthenic acids in recovered crude oil was studied using heavy oil from Usinskoye oil field (Republic Komi), which has high density, high content nickel and vanadium porphyrin complexes, the oxygen compounds and naphthenic acids. It is found that EOR system in combination with thermal steam treatment injection brings about an increase in the content of vanadium porphyrin complexes of crude oils. As a result of the oil-displacement EOR system injection, the naphthenic acids content would decrease in heavy oils.

scholarly journals Biogeochemical Analyses of Four Crude Oil Samples to Confirm the Presence of Terrestrial-organic Matter, Collected from the Murzuq Basin- Libya

2020 ◽  
pp. 43-50
Author(s):  
S. Aboglila ◽  
M. Ramadan ◽  
A. Alburki ◽  
A. Albaghdady
Keyword(s):  
Organic Matter ◽  
Crude Oil ◽  
Oil Fields ◽  
Crude Oils ◽  
Terrestrial Organic Matter ◽  
Bulk Analysis ◽  
Marine Organic Matter ◽  
Terrestrial Sediments ◽  
Wax Content ◽  
Ph Range

The Murzuq Basin, in Southwestern Libya, is one of Libyan’s most productive petroleum basins, with reserves estimated at 23 billion barrels recoverable of hydrocarbon equivalent within giant oil fields. Four crude oil samples collected from A1-NC186, E1-NC101, E2-NC101, H10-NC115 wells from Murzuq Basin, Libya, were suggested for bulk analysis and physical proprieties, alkane distribution and isoprenoids and the hopane, tricyclic terpane and sterane distributions of the oils, to confirm the presence of non-marine organic matter sources and/or any evidence of its existence. The oils show no significant variation in the ratios of the carbon, hydrogen, nitrogen and sulfur content (CHNS) composition. All samples recorded high to very high API° gravity, ranges 33.8 to 40.3; the sulfur values ranged from 0.31 to 0.45%, the wax ratio in crude oils is showing a great variety, E1-NC101and E2-NC101 oils show higher wax content, ranging from 6.1 to 6.7%. Pristine to Phytane ratios (Pr/Ph) range from 1.61 to 2.22 and the Pr/n-C17, Ph/n-C18, n-C17/n-C27 and CPI values determined as indicators of the depositional environment of the crude oils. The data of search confirmed that the crude oils were derived from marine, mixed and terrestrial sediments.

scholarly journals Natural Radionuclide Analysis on Crude Petroleum from Some Oil Fields in Ghana

2021 ◽  
pp. 41-50
Author(s):  
Calvin Kwesi Gafrey ◽  
Robert Wilson ◽  
George Amoako ◽  
Benjamin Anderson
Keyword(s):  
Crude Oil ◽  
International Atomic Energy Agency ◽  
Activity Concentration ◽  
Oil Field ◽  
Atomic Energy ◽  
Oil Fields ◽  
Crude Oils ◽  
Crude Petroleum ◽  
Energy Agency ◽  
Naturally Occurring

The petroleum industries benefit immensely from developing scientific processes and procedures that determine characteristics of crude oils and distinct them. This is because crude oils are acquired from various geological sources and each sedimentary rock has its own distinct chemical properties that affect extraction, refinery and environmental safety. Extraction of crude oils may result in exposure of some radionuclide elements from sedimentary rocks into the environments. This nuclear exposure may affect lives and can cause ecological imbalance. Applications of gamma emission spectroscopy has grown rapidly around the world because of its accurate measurement and precision. Such technique has not been applied for the study of naturally occurring radionuclide materials (NORMs) in crude oils from the active oil fields in Ghana. The study employed the use of gamma spectroscopic technique for characterization of crude oil samples from Jubilee Oil Field, Tweneboa Enyenra Ntomme (TEN) Oil Field and Saltpond Oil Field. The technique was applied on four crude oil samples. The results of this paper actually report on radionuclide concentrations of naturally occurring radioactive materials in four crude samples using a Gamma-Ray spectrometer. Three radionuclides, radium-226 (226 Ra), thorium-232 (232 Th) and potassium-40 (40 K), were identified from each crude sample (JF, TF SF-1 and SF-2). It was observed that samples from Saltpond Oil Field estimated the highest activity concentration and the least was crude oil from Jubilee Oil Field. The activity concentration values were far less than the approved values by International Atomic Energy Agency for basic safety standard. Therefore, the radionuclides activity concentration values of crude oil samples from Jubilee, TEN, and Saltpond oil fields were within the basic safety standards exemption levels of the International Atomic Energy Agency (IAEA). The crude oils from the oil fields can therefore be considered safe to handle. Also, the results of the activity concentrations from the sample used for this study indicate that crude petroleum from the oil fields may relatively have little nuclear activity impact to ecology and human health. The result of this study may be adopted by the Environmental Protection Agency and the Petroleum Stakeholders of Ghana for development of petroleum geo-radionuclides radiation safety measures.

Implementation of an Integrated Geochemical Approach Using Polar and Nonpolar Components of Crude Oil for Reservoir-Continuity Assessment: Verification with Reservoir-Engineering Evidences

SPE Journal ◽  
2021 ◽  
pp. 1-18
Author(s):  
Morteza Asemani ◽  
Ahmad Reza Rabbani ◽  
Hashem Sarafdokht
Keyword(s):  
Crude Oil ◽  
Pairwise Comparison ◽  
Oil Field ◽  
Reservoir Engineering ◽  
Crude Oils ◽  
Worst Case ◽  
Dezful Embayment ◽  
Polar Components ◽  
Reservoir Compartmentalization ◽  
Geochemical Approach

Summary The ability of geochemistry techniques in reservoir-continuity studies has already been proved. Most of the traditional methods mainly involve analyzing nonpolar components of crude oil and overlooking polar components. Despite valuable information obtained from nonpolar components, these compounds are sometimes affected by various alterations or likely provide only a piece of the reservoir-compartmentalization puzzle. In this paper, an integrated geochemical approach that uses nonpolar (i.e., saturates and aromatics) and polar (i.e., asphaltenes) components of crude oil was performed to evaluate reservoir continuity efficiently. The Shadegan Oil Field in the Dezful Embayment in southwest Iran was investigated for reservoir-continuity studies to show the efficiency of this proposed technique. The selected interparaffin peak ratios and light hydrocarbons [the C7 oil correlation star diagram (C7CSD)] from whole-oil gas chromatography (GC) (WOGC) chromatograms were used to obtain oil fingerprints from the nonpolar fraction of crude oils. The Fourier-transform infrared (FTIR) spectroscopy of asphaltenes was applied to obtain oil fingerprints from the polar fraction of crude oils. The pairwise comparison of studied wells by each technique was summarized in a similarity matrix with green, yellow, and red colors to show connectivity, limited connectivity, and disconnectivity according to oil fingerprints. Finally, a compartmentalization model was prepared from the integrated results of different techniques considering the worst-case scenarios regarding the occurrence or absence of reservoir continuity when relying on individual methods for the studied field. Results show that the Shadegan Oil Field comprises three zones in the Asmari Reservoir and two zones in the Bangestan Reservoir. Reservoir-engineering data, including pressure data and pressure/volume/temperature (PVT), completely corroborated the obtained results from the geochemical approach. The consistency of results suggested FTIR oil fingerprinting of asphaltene as a novel and straightforward technique, which is a complementary or even alternative method with respect to previous geochemical methods.

scholarly journals Investigation of crude oils compatibility and relationship with their properties

2019 ◽  
Vol 20 (K8) ◽  
pp. 35-41
Author(s):  
Huynh Minh Thuan ◽  
Nguyen Manh Huan ◽  
Vo Thi Thuong ◽  
Nguyen Thi Nhi ◽  
Tran Ky Anh ◽  
...  
Keyword(s):  
Crude Oil ◽  
Chemical Properties ◽  
Crude Oils ◽  
Asphaltene Precipitation ◽  
Physical Chemical ◽  
Physico Chemical ◽  
Wax Content ◽  
Oil Tanks ◽  
The Relationship

The compatibility/incompatibility of crude oils will affect the asphaltene precipitation and finally for sludge deposition if such oils are storaged or blending together. Normally, the refineries process a mixture of several crude oils owing to economic, technical and political aspects. In this study, four crude oils were characterized and investigated in order to determine physical-chemical properties and insolubility number (IN) and blending solubility number (SBN). The result revealed that two pair of crude oils are fully compatible and the remaining are partly compatible. The volume limitation of each crude oil in the mixture is proposed. In addition, the relationship between the compatibility and physico-chemical properties of crude oils is discussed. In fact, the crude oil which possessed high wax content and low resins to asphaltenes ratio possesses low stability. This finding might provide a new and valuable strategy for solving the foulings in crude oil tanks and processing units in refineries.  

Study of the Effect of Crude Oils on the Metallic Corrosion

2018 ◽  
Vol 5 (1) ◽  
pp. 43-54
Author(s):  
Suresh Aluvihara ◽  
Jagath K Premachandra
Keyword(s):  
Crude Oil ◽  
Sulfur Content ◽  
Salt Content ◽  
Ferrous Metal ◽  
Relative Weight ◽  
Oil Refining ◽  
Crude Oils ◽  
Hardness Tester ◽  
Corrosion Rates ◽  
Ferrous Metals

Corrosion is a severe matter regarding the most of metal using industries such as the crude oil refining. The formation of the oxides, sulfides or hydroxides on the surface of metal due to the chemical reaction between metals and surrounding is the corrosion that  highly depended on the corrosive properties of crude oil as well as the chemical composition of ferrous metals since it was expected to investigate the effect of Murban and Das blend crude oils on the rate of corrosion of seven different ferrous metals which are used in the crude oil refining industry and investigate the change in hardness of metals. The sulfur content, acidity and salt content of each crude oil were determined. A series of similar pieces of seven different types of ferrous metals were immersed in each crude oil separately and their rates of corrosion were determined by using their relative weight loss after 15, 30 and 45 days. The corroded metal surfaces were observed under the microscope. The hardness of each metal piece was tested before the immersion in crude oil and after the corrosion with the aid of Vicker’s hardness tester. The metallic concentrations of each crude oil sample were tested using atomic absorption spectroscopy (AAS). The Das blend crude oil contained higher sulfur content and acidity than Murban crude oil. Carbon steel metal pieces showed the highest corrosion rates whereas the stainless steel metal pieces showed the least corrosion rates in both crude oils since that found significant Fe and Cu concentrations from some of crude oil samples. The mild steel and the Monel showed relatively intermediate corrosion rates compared to the other types of ferrous metal pieces in both crude oils. There was a slight decrease in the initial hardness of all the ferrous metal pieces due to corrosion.

scholarly journals A Simple and Accurate Approach for Determining the VFA Concentration in Anaerobic Digestion Liquors, Relying on Two Titration Points and an External Inorganic Carbon Analysis

2021 ◽  
Vol 5 (2) ◽  
pp. 15
Author(s):  
Paz Nativ ◽  
Yonatan Gräber ◽  
Yaron Aviezer ◽  
Ori Lahav
Keyword(s):  
Partial Pressure ◽  
Volatile Fatty Acids ◽  
Inorganic Carbon ◽  
Strong Acid ◽  
Direct Analysis ◽  
Anaerobic Digesters ◽  
Acid Titration ◽  
Partial Pressure Of Co2 ◽  
Ph Range

A new analytic approach is presented for determining the total volatile fatty acids (VFAT) concentration in anaerobic digesters. The approach relies on external determination of the inorganic carbon concentration (CT) in the analyzed solution, along with two strong-acid titration points. The CT concentration can be determined by either a direct analysis (e.g., by using a TOC device) or by estimating it from the recorded partial pressure of CO2(g) in the biogas (often a routine analysis in anaerobic digesters). The titration is carried out to pH 5.25 and then to pH 4.25. The two titration results are plugged into an alkalinity-mass-based equation and then the two terms are subtracted from each other to yield an equation in which VFAT is the sole unknown (since CT is known and the effect of the total orthophosphate and ammonia concentrations is shown to be small at this pH range). The development of the algorithm and its verification on four anaerobic reactor liquors is presented, on both the raw water and on acetic acid-spiked samples. The results show the method to be both accurate (up to 2.5% of the expected value for VFAT/Alkalinity >0.2) and repetitive when the total orthophosphate and ammonia concentrations are known, and fairly accurate (±5% for VFAT >5 mM) when these are completely neglected. PHREEQC-assisted computation of CT from the knowledge of the partial pressure of CO2(g) in the biogas (and pH, EC and temperature in the liquor) resulted in a very good estimation of the CT value (±3%), indicating that this technique is adequate for the purpose of determining VFAT for alarming operators in case of process deterioration and imminent failure.

Sign in / Sign up

Export Citation Format

Share Document