Phylogeny based on 16S rDNA andnifHsequences ofRalstonia taiwanensisstrains isolated from nitrogen-fixing nodules ofMimosa pudica, in India

2004 ◽  
Vol 50 (5) ◽  
pp. 313-322 ◽  
Author(s):  
Subhash Chandra Verma ◽  
Soumitra Paul Chowdhury ◽  
Anil Kumar Tripathi
Keyword(s):  
16S Rdna ◽  
South India ◽  
Restriction Analysis ◽  
Cross Reaction ◽  
North India ◽  
Mimosa Pudica ◽  
Free Living ◽  
South Indian ◽  
Indian Isolates ◽  
Dinitrogenase Reductase

Bacterial symbionts present in the indeterminate-type nitrogen (N)-fixing nodules of Mimosa pudica grown in North and South India showed maximum similarity to Ralstonia taiwanensis on the basis of carbon-source utilization patterns and 16S rDNA sequence. Isolates from the nodules of M. pudica from North India and South India showed identical ARDRA (Amplified Ribosomal DNA Restriction Analysis) patterns with Sau3AI and RsaI, but AluI revealed dimorphy between the North Indian and South Indian isolates. Alignment of 16S rDNA sequences revealed similarity of North Indian isolates with an R. taiwanensis strain isolated from M. pudica in Taiwan, whereas South Indian isolates showed closer relatedness with the isolates from Mimosa diplotricha. Alignment of nifH sequences from both North Indian and South Indian isolates with that of the related isolates revealed their closer affinity to α-rhizobia, suggesting that nif genes in the β-rhizobia might have been acquired from α-rhizobia via lateral transfer during co-occupancy of nodules by α-rhizobia and progenitors of R. taiwanensis, members of the β-subclass of Proteobacteria. Immunological cross-reaction of the bacteroid preparation of M. pudica nodules showed strong a positive signal with anti-dinitrogenase reductase antibody, whereas a weak positive cross-reaction was observed with free-living R. taiwanensis grown microaerobically in minimal medium with and without NH4Cl. In spite of the expression of dinitrogenase reductase under free-living conditions, acetylene reduction was not observed under N-free conditions even after prolonged incubation.Key words: symbiotic nitrogen fixation, Mimosa pudica, rhizobia, phylogeny, 16S rDNA, nifH, Ralstonia taiwanensis.

scholarly journals Diversity of Free-Living and Attached Bacteria in Offshore Western Mediterranean Waters as Depicted by Analysis of Genes Encoding 16S rRNA

1999 ◽  
Vol 65 (2) ◽  
pp. 514-522 ◽  
Author(s):  
Silvia G. Acinas ◽  
Josefa Antón ◽  
Francisco Rodríguez-Valera
Keyword(s):  
16S Rdna ◽  
Restriction Analysis ◽  
Western Mediterranean ◽  
Bacterial Cells ◽  
Free Living ◽  
Attached Bacteria ◽  
Genes Encoding ◽  
Community Fingerprinting ◽  
Close Relatives ◽  
Alteromonas Macleodii

ABSTRACT In a previous study (S. G. Acinas, F. Rodrı́guez-Valera, and C. Pedrós-Alió, FEMS Microbiol. Ecol. 24:27–40, 1997), community fingerprinting by 16S rDNA restriction analysis applied to Mediterranean offshore waters showed that the free-living pelagic bacterial community was very different from the bacterial cells aggregated or attached to particles of more than about 8 μm. Here we have studied both assemblages at three depths (5, 50, and 400 m) by cloning and sequencing the 16S rDNA obtained from the same samples, and we have also studied the samples by scanning electron microscopy to detect morphology patterns. As expected, the sequences retrieved from the assemblages were very different. The subsample of attached bacteria contained very little diversity, with close relatives of a well-known species of marine bacteria, Alteromonas macleodii, representing the vast majority of the clones at every depth. On the other hand, the free-living assemblage was highly diverse and varied with depth. At 400 m, close relatives of cultivated γProteobacteria predominated, but as shown by other authors, near the surface most clones were related to phylotypes described only by sequence, in which the α Proteobacteria of the SAR11 cluster predominated. The new technique of rDNA internal spacer analysis has been utilized, confirming these results. Clones representative of the A. macleodii cluster have been completely sequenced, producing a picture that fits well with the idea that they could represent a genus with at least two species and with a characteristic depth distribution.

TRACE ELEMENT ANALYSIS OF SOUTH INDIAN GALLSTONES BY PIXE

2002 ◽  
Vol 12 (03n04) ◽  
pp. 137-144 ◽  
Author(s):  
M. ASHOK ◽  
S. NARAYANA KALKUPA ◽  
V. J. KENNEDY ◽  
A. MARKWITZ ◽  
V. JAYANTHI ◽  
...  
Keyword(s):  
South India ◽  
Elemental Concentration ◽  
Trace Element Analysis ◽  
North India ◽  
Element Analysis ◽  
Cholesterol Gallstones ◽  
South Indian ◽  
Pigment Gallstones ◽  
Copper Zinc ◽  
Experimental Subjects

Particle Induced X-ray Emission (PIXE) was used to analyse the elemental concentration of gallstones collected from different parts of south India. Cholesterol gallstones are predominant in north India, whereas pigment gallstones are predominant in south India. The black pigment gallstones are common in hemolytic anemia or in presence of infected bile. PIXE has revealed the elemental profile in each sample. The experimental subjects of the different groups displayed significant variation in their levels of certain trace elements such as copper, zinc, and iron. The concentration of copper was found to be predominant in pigment gallstones investigated in the present study.

Hindustani Classical Music in Sri Lanka: A Dominating Minority Music or an Imposed Musical Ideology?

2020 ◽  
Vol 6 ◽  
pp. 41-50
Author(s):  
Chinthaka Prageeth Meddegoda
Keyword(s):  
Sri Lanka ◽  
Performing Arts ◽  
South India ◽  
Classical Music ◽  
Minority Group ◽  
North India ◽  
13Th Century ◽  
South Indian ◽  
Indian Music ◽  
Hindustani Classical Music

In Sri Lanka, the various groups of Tamils are jointly the largest minority group who migrated from different places of South India and in different time periods. South Indian music is widely appreciated and learnt by both the Sinhala including by large parts of the Tamil minority spread over Sri Lanka. Although a number of Sinhala people prefer and practice North Indian music geographically, and probably culturally, they are much closer to South India than to North India. Some historical sources report that Sinhalese are descendants of North Indians who are believed to be Aryans who migrated from Persia to the Northern part of India in the 13th century and later. Therefore, some scholarly authorities believe that the Sinhalese ‘naturally’ prefer North Indian music as they also continue the suggested Aryan heritage. Nevertheless, some other sources reveal that the North Indian music was spread in Sri Lanka during the British rule with the coming of the Parsi Theatre (Bombay theatre), which largely promoted Hindustani raga-based compositions. This paper explores selected literature and opinions of some interviewees and discusses what could be the reasons for preferences of North Indian music by the Sinhalese. The interviewees were chosen according to their professional profile and willingness to participate in this research. As a result, this paper will offer insights through analysing various opinions and statements made by a number of interviewees. The research also considered some theories which may relate to the case whether Hindustani classical music is due to these reasons a dominating minority culture or a rather self-imposed musical ideology. The latter would establish an aesthetic hierarchy, which is not reflected in the cultural reality of Sri Lanka. This is a new research scrutinizing a long-term situation of performing arts education in this country taking mainly interviews as a departing point.

scholarly journals Diversity in the cag pathogenicity island of Helicobacter pylori isolates in populations from North and South India

2010 ◽  
Vol 59 (1) ◽  
pp. 32-40 ◽  
Author(s):  
Sushil Kumar ◽  
Ashok Kumar ◽  
Vinod Kumar Dixit
Keyword(s):  
Helicobacter Pylori ◽  
South India ◽  
Rflp Analysis ◽  
Variable Region ◽  
Pathogenicity Island ◽  
North India ◽  
South Indian ◽  
Pcr Rflp ◽  
H Pylori ◽  
North And South

The cag pathogenicity island (cagPAI) has been reported to be the major virulence determinant in Helicobacter pylori-related diseases. In the present study, the diversity of the cagA gene and the integrity of the cagPAI in 158 H. pylori strains from Varanasi (North India) and Hyderabad (South India) were studied by amplifying the cagA gene (∼3.5 kb), followed by PCR-RFLP analysis. The results revealed significant differences in the cagA gene and the integrity of the cagPAI between North and South Indian isolates. Of 158 isolates, 40 (34.8 %) from Varanasi and 20 (46.5 %) from Hyderabad were found to carry an intact cagPAI. A partially deleted cagPAI was present in 75 (65.2 %) isolates from Varanasi and 23 (53.5 %) from Hyderabad. None of the isolates showed complete deletion of the cagPAI. Differences in the cagA 5′ and 3′ regions were also noted, and 11 isolates (8 from Varanasi and 3 from Hyderabad) that were cagA negative with primers for the 5′ region turned out to be cagA positive with primers for the 3′ variable region. It is tentatively concluded that the 3′ variable region may be a better marker for cagA typing. The results also showed that the majority of the isolates harboured the Western-type EPIYA motif. PCR-RFLP analysis of the cagA gene showed 29 distinguishable digestion patterns, and cluster analysis of RFLP types from a random selection of 32 isolates placed all of the isolates into 5 groups. These results demonstrate that significant differences in the cagPAI occur among isolates from North and South India, and that RFLP of cagA could be employed for elucidating genetic variations among various isolates of H. pylori.

scholarly journals RELATION OF ANCIENT MATARAM TEMPLE BASED ON NORTH INDIA AND SOUTH INDIA’S CHARACTERISTICS: FROM THE FIGURE-TECTONICS, MASS-SPACE STUDY, AND ORNAMENTATION

2021 ◽  
Vol 5 (04) ◽  
pp. 334-347
Author(s):  
Samuel H. B. Danuleksono ◽  
Rahadhian Prajudi Herwindo
Keyword(s):  
Spatial Planning ◽  
South India ◽  
Reverse Flow ◽  
North India ◽  
Traditional Values ◽  
Central Java ◽  
South Indian ◽  
Close Relationship ◽  
The Relationship ◽  
North And South

Abstract- Hinduism is a religion that was born in India and has developed rapidly into the archipelago with evidence of Sanskrit inscriptions and Pallava. The religion had a significant influence on Java which changed the political, economic, social and cultural life which at that time was still a kingdom. The arrival of Indians to Indonesia affected by the reverse flow between Indonesia and India and India and Indonesia. This can be seen by the presence of Indonesian dormitories in Nalanda (North India) and Nagapattinam (South India). The influence of North and South India can be seen from the existence of Hindu-Buddhist temples in the Archipelago which was built in one of the Hindu kingdoms, namely the Kingdom of Ancient Mataram which had areas of authority from Central Java to East Java. The temples that were built are thought to have an identity with temples in North and South India in terms of figure, inner space tectonics, mass layout, spatial planning, and ornamentation. This identity is thought to be a close relationship between North India and South India with Indonesia, especially in the era of Ancient Mataram. The purpose of this study is to find out the relationship or relationship of Ancient Mataram era temple architecture to North and South Indian temple architecture. The analysis method used is the comparative – qualitative method. Work plans, plans, pieces, site plans, and block plans of 28 Hindu-Buddha tower types in Central Java will be compared in terms of similarities and differences and then analyzed regarding the position of the laying, and elements of the temple so that the relationship between the two countries is found. Data obtained from literature studies and field surveys. The conclusion drawn from this study is that there is a closer relationship with South India than North India. The influence of North India and South India is only limited to the external appearance in the study of figure and ornamentation, while in the study of mass and spatial planning and inner space tectonics is more developed by the people of Ancient Mataram which is adjusted to traditional values and natural influences.     Key Words: figure, tectonics, mass layout, spatial planning, ornamentation, North and South India, Ancient Mataram

scholarly journals An Assessment of Skeletal Craniofacial Asymmetry in South Indian Population

2012 ◽  
Vol 13 (1) ◽  
pp. 80-84 ◽  
Author(s):  
G Anil Kumar ◽  
Saibel Farishta ◽  
G Baiju ◽  
VK Taneja ◽  
RC Minocha ◽  
...  
Keyword(s):  
College Students ◽  
South India ◽  
Indian Population ◽  
Cranial Base ◽  
The Other ◽  
South Indian Population ◽  
X Rays ◽  
Base Area ◽  
South Indian ◽  
The Face

ABSTRACT The present study was undertaken to assess the skeletal craniofacial asymmetry in South Indian population by a posteroanterior cephalometric radiographic method. The skeletal craniofacial structures on one side of the face were compared with that of the other, by drawing various triangles representing different craniofacial regions. The sample consisted of 60 subjects (30 males and 30 females) aged between 18 to 25 years, who were mainly dental college students from South India. Overall 52 X-rays were obtained, with four errors each in the male and the female groups. The results revealed that the total facial structures in the South Indian population were larger on the left side (statistically insignificant). The cranial base area exhibited a greater degree of asymmetry than any other component area of the face, which might be due to the inaccuracy at the condylar point. How to cite this article Taneja VK, Kumar GA, Farishta S, Minocha RC, Baiju G, Gopal D. An Assessment of Skeletal Craniofacial Asymmetry in South Indian Population. J Contemp Dent Pract 2012;13(1):80-84.

Determinants of TB Incidence in South Indian Population: Chengalpattu BCG Trial Revisited, South India

2021 ◽  
Author(s):  
Mohan Kumar Papanna ◽  
Basilea Watson ◽  
Radhamani MP ◽  
Soumya S. Swaminathan ◽  
Manjula Datta ◽  
...  
Keyword(s):  
South India ◽  
Indian Population ◽  
South Indian Population ◽  
South Indian

scholarly journals Legacy of draught cattle breeds of South India: Insights into population structure, genetic admixture and maternal origin

PLoS ONE ◽  
2021 ◽  
Vol 16 (5) ◽  
pp. e0246497
Author(s):  
Vandana Manomohan ◽  
Ramasamy Saravanan ◽  
Rudolf Pichler ◽  
Nagarajan Murali ◽  
Karuppusamy Sivakumar ◽  
...  
Keyword(s):  
Population Structure ◽  
Population Size ◽  
Effective Population Size ◽  
South India ◽  
Genetic Admixture ◽  
Zebu Cattle ◽  
Effective Population ◽  
Cattle Breeds ◽  
South Indian ◽  
Indian Cattle

The present study is the first comprehensive report on diversity, population structure, genetic admixture and mitochondrial DNA variation in South Indian draught type zebu cattle. The diversity of South Indian cattle was moderately high. A significantly strong negative correlation coefficient of -0.674 (P<0.05) was observed between the effective population size of different breeds and their estimated FIS. The genetic structure analysis revealed the distinctness of Kangayam, Vechur and Punganur cattle from the rest of the zebu breeds. The results showed the influence of Hallikar breed in the development of most Mysore type cattle breeds of South India with the exception of Kangayam. Bayesian clustering analysis was performed to assess the taurine admixture in South Indian zebu cattle using purebred Jersey and Holstein-Friesian as reference genotypes. Relatively high levels of taurine admixture (>6.25%) was observed in Punganur, Vechur, Umblachery and Pulikulam cattle breeds. Two major maternal haplogroups, I1 and I2, typical of zebu cattle were observed, with the former being predominant than the later. The pairwise differences among the I2 haplotypes of South Indian cattle were relatively higher than West Indian (Indus valley site) zebu cattle. The results indicated the need for additional sampling and comprehensive analysis of mtDNA control region variations to unravel the probable location of origin and domestication of I2 zebu lineage. The present study also revealed major concerns on South Indian zebu cattle (i) risk of endangerment due to small effective population size and high rate of inbreeding (ii) lack of sufficient purebred zebu bulls for breeding and (iii) increasing level of taurine admixture in zebu cattle. Availability of purebred semen for artificial insemination, incorporation of genomic/molecular information to identify purebred animals and increased awareness among farmers will help to maintain breed purity, conserve and improve these important draught cattle germplasms of South India.

scholarly journals A Study on Jainism History, Philosophy and Traditions in Tamilnadu

2021 ◽  
Vol 8 (4) ◽  
pp. 75-79
Author(s):  
M Rajeshwari ◽  
A Amirthavalli
Keyword(s):  
Tamil Nadu ◽  
South India ◽  
Huge Amount ◽  
Indian History ◽  
South Indian ◽  
Way Of Thinking ◽  
Made In ◽  
The Way

In Tamil Nadu Hinduism and Buddhism, Jainism is one of the three oldest Indian strict conventions still in presence and a necessary piece of South indian strict conviction and practice. While frequently utilizing ideas imparted to Hinduism and Buddhism, the consequence of a typical social and phonetic foundation, the Jain convention should be viewed as a free marvel as opposed to as a Hindu order or a Buddhist blasphemy, as some previous Western researchers accepted. In South India, Jainism is minimal in overflow of a name. Indeed, even genuine understudies of religion in India gave little consideration to it. In a populace of almost 60 crores of individuals, Jainas may establish almost nearly 3 million individuals. Jainism is the religion of the Jains who follow the way, lectured and rehearsed by the Jinas. It is a fully evolved and grounded religion and social framework that rose up out of 6 century BC .The trademark highlight of this religion is its case to all inclusiveness which it holds essentially contrary to Brahmanism. It very well may be said that throughout the previous 2500 years the Jains have contributed such a huge amount to each circle of life of Indian individuals both as a religion and a way of thinking. They contributed a lot to the regions of culture, language, exchange and agribusiness, or all in all the Jains opened up another period of human thoughts and musings. In Indian History, endeavors were made to contemplate Jainism as a religion and its commitments yet focus on the Jain movement into Tamil Nadu and its effects are restricted. An endeavor is made in this examination to investigate the recorded geology of the Jain focuses in Tamil Nadu.

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