Hybridization between Picearubens and Piceamariana: differences observed between montane and coastal island populations

1996 ◽  
Vol 26 (3) ◽  
pp. 444-452 ◽  
Author(s):  
Michael S. Bobola ◽  
Kimberly A. Hillenberg ◽  
Steve B. Gendreau ◽  
Robert T. Eckert ◽  
Anita S. Klein ◽  
...  
Keyword(s):  
Discriminant Function ◽  
Black Spruce ◽  
Red Spruce ◽  
Nuclear Ribosomal Dna ◽  
Island Populations ◽  
Provenance Test ◽  
Chloroplast Haplotypes ◽  
Natural Stands ◽  
Hybridization And Introgression ◽  
Mount Washington

Foliage was collected from natural stands of montane and island red spruce (Picearubens Sarg.) and black spruce (Piceamariana (Mill.) BSP) to examine within- and among-population genetic variation. Samples were scored for frequencies of nuclear ribosomal DNA (rDNA) alleles, and mitochondrial and chloroplast haplotypes. Samples were classified as red spruce, black spruce, or hybrid using two molecular methods: a three-character discriminant function based on molecular markers or a three-character molecular index. These results were found to be highly congruent with classification based upon a discriminant function using morphological traits. Among montane populations, hybridization and introgression between red and black spruce did not appear to be a major factor in the observed patterns of variation on elevational transects on Mount Washington and Mount Lafayette, N.H. However, extensive hybridization and introgression were detected among populations on Isle au Haut, Maine. The Mount Lafayette population displayed low variation in rDNA alleles compared with populations on Mount Washington and a range-wide provenance test in Stewartstown, N.H.

A provenance test of red spruce in nine environments in eastern Canada

1981 ◽  
Vol 11 (1) ◽  
pp. 124-131 ◽  
Author(s):  
E. K. Morgenstern ◽  
A. G. Corriveau ◽  
D. P. Fowler
Keyword(s):  
North Carolina ◽  
New Brunswick ◽  
Black Spruce ◽  
Introgressive Hybridization ◽  
Red Spruce ◽  
Regression Analyses ◽  
Eastern Canada ◽  
Southern Appalachian ◽  
Provenance Test ◽  
Seed Origin

Survival and total height of red spruce (Picearubens Sarg.) at ages 15 and 22 years from seed are reported. Twelve provenances distributed from North Carolina to Quebec were grown in three experiments each in Ontario, Quebec, and New Brunswick; seven additional provenances were only partially represented. Variance, correlation, and regression analyses were made. Results at both ages were very similar. Provenance differences in survival were small at individual sites and significant only when the results from all nine sites were combined. Provenance differences in height were well expressed and significant in each of the three groups of tests, with northern provenances growing best. Several provenances were also relatively stable and performed well from site to site. Correlation and regression analyses showed that variation in height was more closely related to the degree of introgressive hybridization with black spruce (Piceamariana (Mill.) B.S.P.) than to latitude, elevation, or precipitation at the place of seed origin. These results were conditioned by development on open sites which are not typical red spruce sites.In contrast with expectations when the study was initiated, it is now apparent that provenances from the southern Appalachian Mountains in West Virginia and North Carolina are less variable than expected and not suitable for reforestation in Canada.

Restriction fragment variation in the nuclear ribosomal DNA repeat unit within and between Picearubens and Piceamariana

1992 ◽  
Vol 22 (2) ◽  
pp. 255-263 ◽  
Author(s):  
Michael S. Bobola ◽  
Robert T. Eckert ◽  
Anita S. Klein
Keyword(s):  
Restriction Fragment ◽  
Ribosomal Dna ◽  
Black Spruce ◽  
Repeat Unit ◽  
Red Spruce ◽  
Classification Model ◽  
Nuclear Ribosomal Dna ◽  
Restriction Fragments ◽  
Dna Repeat ◽  
Polymorphic Restriction

The frequencies of polymorphic restriction fragments for the nuclear ribosomal DNA repeat were compared for 12 provenances of red spruce (Picearubens Sarg.) and 34 provenances of black spruce (Piceamariana (Mill.) B.S.P.). Within an individual as many as five distinct ribosomal DNA repeat unit types could be distinguished. Canonical correlation analysis revealed significant variation of restriction fragment frequencies with a geographic variate comprising latitude and longitude of provenances. Geographic origins accounted for 24.7% of the variation in polymorphic restriction fragments in black spruce and 31.8% of the variation in polymorphic restriction fragments in red spruce. Discriminant analysis, using the restriction fragment frequencies for the ribosomal DNA, was used to develop a classification model for the two species. Tenfold verification of the model produced an average correct classification of 99% for black spruce and 96% for red spruce. Plots of canonical scores for the first and second canonical variâtes clearly separated red spruce from black spruce. This study presents a novel combination of restriction fragment frequency data and multivariate analysis to distinguish species that may not always be differentiated using morphological traits.

Breeding implications of juvenile selection in a range-wide black spruce provenance test

1984 ◽  
Vol 14 (6) ◽  
pp. 933-939 ◽  
Author(s):  
Hans Nienstaedt
Keyword(s):  
Large Range ◽  
Black Spruce ◽  
Field Tests ◽  
Simple Correlation ◽  
Growing Seasons ◽  
Breeding Populations ◽  
Provenance Test ◽  
Full Complement ◽  
Growth Initiation ◽  
Growth Differences

Using 3-year heights, the tallest 49 provenances were selected in a nursery test of 110 black spruce (Piceamariana (Mill.) B.S.P.) provenances. A field test of the selected provenances was planted in Wisconsin; two tests of 108 provenances, both in Minnesota. Characteristics evaluated in the nursery included heights after two, three, and five growing seasons, and growth initiation and cessation in the second and third growing season. Heights were measured in the field tests. Separate statistical analyses, ANOVA and simple correlation, were used for the full complement of provenances and for those that were selected. All nursery characteristics varied significantly; the correlations between latitude and height decreased with age, and were not significant after five season's growth. Differences among provenances in free growth may explain this. Selection at age 3 years was not effective; too many selection errors were evident in the test of the full complement of provenances. Early selection on the basis of heights and phenological characteristics is not an effective way of reducing large range-wide provenance tests to breeding populations.

Natural hybridization between black spruce and red spruce

1997 ◽  
Vol 6 (8) ◽  
pp. 725-734 ◽  
Author(s):  
M. Perron ◽  
J. Bousquet
Keyword(s):  
Black Spruce ◽  
Red Spruce ◽  
Natural Hybridization

scholarly journals Light-energy processing and freezing-tolerance traits in red spruce and black spruce: species and seed-source variation

2003 ◽  
Vol 23 (10) ◽  
pp. 685-694 ◽  
Author(s):  
J. E. Major ◽  
D. C. Barsi ◽  
A. Mosseler ◽  
M. Campbell ◽  
O. P. Rajora
Keyword(s):  
Freezing Tolerance ◽  
Black Spruce ◽  
Light Energy ◽  
Red Spruce ◽  
Seed Source ◽  
Energy Processing ◽  
Source Variation

A high-density genetic linkage map of a black spruce (Picea mariana) × red spruce (Picea rubens) interspecific hybrid

Genome ◽  
2011 ◽  
Vol 54 (2) ◽  
pp. 128-143 ◽  
Author(s):  
Bum-Yong Kang ◽  
John E. Major ◽  
Om P. Rajora
Keyword(s):  
Picea Mariana ◽  
Interspecific Hybrid ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Black Spruce ◽  
Picea Rubens ◽  
Red Spruce ◽  
Linkage Groups ◽  
Genomic Resource ◽  
Map Distance

Genetic maps provide an important genomic resource of basic and applied significance. Spruce ( Picea ) has a very large genome size (between 0.85 × 1010 and 2.4 × 1010 bp; 8.5–24.0 pg/1C, a mean of 17.7 pg/1C ). We have constructed a near-saturated genetic linkage map for an interspecific backcross (BC1) hybrid of black spruce (BS; Picea mariana (Mill.) B.S.P.) and red spruce (RS; Picea rubens Sarg.), using selectively amplified microsatellite polymorphic loci (SAMPL) markers. A total of 2284 SAMPL markers were resolved using 31 SAMPL–MseI selective nucleotide primer combinations. Of these, 1216 SAMPL markers showing Mendelian segregation were mapped, whereas 1068 (46.8%) SAMPL fragments showed segregation distortion at α = 0.05. Maternal, paternal, and consensus maps consistently coalesced into 12 linkage groups, corresponding to the haploid chromosome number (1n = 1x = 12) of 12 in the genus Picea. The maternal BS map consisted of 814 markers distributed over 12 linkage groups, covering 1670 cM, with a mean map distance of 2.1 cM between adjacent markers. The paternal BS × RS map consisted of 773 markers distributed over 12 linkage groups, covering 1563 cM, with a mean map distance of 2.0 cM between adjacent markers. The consensus interspecific hybrid BC1 map consisted of 1216 markers distributed over 12 linkage groups, covering 1865 cM (98% genome coverage), with a mean map distance of 1.5 cM between adjacent markers. The genetic map reported here provides an important genomic resource in Picea, Pinaceae, and conifers.

scholarly journals Density Management Diagram for Northeastern Red Spruce and Balsam Fir Forests

1999 ◽  
Vol 16 (1) ◽  
pp. 48-56 ◽  
Author(s):  
Duncan S. Wilson ◽  
Robert S. Seymour ◽  
Douglas A. Maguire
Keyword(s):  
Stand Density ◽  
Maximum Size ◽  
Site Index ◽  
The Self ◽  
Red Spruce ◽  
Balsam Fir ◽  
Stand Development ◽  
Exponential Relationship ◽  
Data Set ◽  
Natural Stands

Abstract A stand-density management diagram is presented for use in northeastern red spruce and balsam fir forests. The diagram was derived from an extensive archived data set collected during the 1970s from fully stocked stands throughout northern Maine and a more recent study of precommercially thinned stands. The negative exponential relationship between mean stemwood volume per tree and stand density, commonly known as the "self-thinning rule, "was formulated to define a biological maximum stand density. The maximum size-density equation can be used to calculate the relative density of any stand and is accurate for thinned and unthinned natural stands as well as plantations. Equations for estimating quadratic mean diameter and stand top height are also derived for unthinned natural stands only. Data used to fit the self-thinning line are substantially above the A-lines on the familiar northeastern stocking guides, suggesting that these guides underestimate maximum density and thus overpredict self-thinning. Examples illustrate how to use the diagram to predict stand development under commercial and precommercial thinning scenarios, as well as natural stand development without thinning. Relevant site index and volume equations are included in an appendix. North. J. Appl. For. 16(1):48-56.

Comparison of isozyme variation among natural stands and plantations: jack pine and black spruce

1985 ◽  
Vol 15 (5) ◽  
pp. 902-908 ◽  
Author(s):  
Peggy Knowles
Keyword(s):  
Black Spruce ◽  
Seed Orchard ◽  
Jack Pine ◽  
Allelic Heterogeneity ◽  
Nonrandom Mating ◽  
Clonal Seed Orchard ◽  
Seed Zone ◽  
Natural Stands ◽  
Mating Conditions ◽  
Seed Collections

Isozymes from needles of jack pine (Pinusbanksiana) and black spruce (Piceamariana) were analysed to test differences among young stands, mature stands, plantations from seed-zone seed collections, a plus-tree clonal seed orchard, and a progeny plantation from the seed orchard. Analyses based on approximately 100 trees per population resolved seven polymorphic loci for jack pine and five for black spruce. For intraspecies comparisons, allelic heterogeneity tests indicated no differences between young stands and plantations from seed-zone seed collections. The black spruce clonal seed orchard and mature natural stands were genetically homogeneous. Allelic heterogeneity test and discriminant analysis indicated that the progeny plantation from the clonal seed orchard differed from other black spruce samples. It is proposed that nonrandom mating conditions in the clonal seed orchard altered allele frequencies of the resultant progeny plantation.

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