Embryolike structures from cotyledons and ripe embryos of Norway spruce (Piceaabies)
1986 ◽
Vol 16
(3)
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pp. 664-668
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Keyword(s):
Embryos from imbibed ripe seeds and cotyledon expiants of 7-day-old Norway spruce (Piceaabies (L.) Karst.) seedlings produced the early stages of somatic embryogenesis. Using a modified Murashige and Skoog medium, a whitish, glossy callus was induced consisting of translucent cells embedded in a mucilaginous cloudy matrix. This embryogenic callus formed on the surface of explants treated first with N-6-benzyladenine followed by 2,4-dichlorophenoxyacetic acid + 6-furfurylaminopurine (kinetin) + N-6-benzyladenine. Transfer of this callus to media lacking growth regulators resulted in the formation of numerous bipolar embryoids with suspensorlike structures. These embryoids strongly resembled repressed embryos in polyembryonic seeds.
2014 ◽
Vol 51
(2)
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pp. 173-178
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1988 ◽
Vol 18
(7)
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pp. 937-941
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1986 ◽
Vol 16
(2)
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pp. 420-422
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1993 ◽
Vol 23
(5)
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pp. 835-838
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2009 ◽
Vol 25
(3)
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