Depressing effects of calcium antagonists on oxygen consumption in isolated skeletal muscle during potassium depolarization

1988 ◽  
Vol 66 (6) ◽  
pp. 836-840 ◽  
Author(s):  
William S. Barnes
Keyword(s):  
Skeletal Muscle ◽  
Oxygen Consumption ◽  
Oxygen Uptake ◽  
Calcium Antagonists ◽  
Metabolic Response ◽  
Potassium Depolarization ◽  
Dantrolene Sodium ◽  
Ringer’S Solution ◽  
Intracellular Ca ◽  
Frog Sartorius

The effects of two Ca2+-antagonizing drugs, dantrolene sodium (DAN) and TMB-8, on subcontracture, K+-induced increased oxygen consumption were studied in isolated frog sartorius muscles. Incubation in Ringer's solution +18 mM KCl caused a marked increase in averaged resting oxygen uptake (52.8 ± 4.1 to 278.2 ± 20.0 mm3∙g−1∙h−1). Incubation in Ringer 's +18 mM KCl containing either 0.116 mM TMB-8 or 0.035 mM DAN resulted in a significant reduction in the averaged metabolic response (264.4 ± 30.8 (control) vs. 142.3 ± 19.0 (TMB-8) vs. 72.2 ± 3.4 (DAN)). The results suggest that the enhanced metabolic rate observed upon elevating extracellular [K+] to just below contracture levels is due to increased intracellular Ca2+ release.

Association of increased pHi with calcium ion release in skeletal muscle

1978 ◽  
Vol 234 (3) ◽  
pp. C110-C114 ◽  
Author(s):  
R. J. Connett
Keyword(s):  
Skeletal Muscle ◽  
Calcium Ion ◽  
Calcium Release ◽  
Muscle Membrane ◽  
Sartorius Muscle ◽  
Ion Release ◽  
Extracellular Medium ◽  
Dantrolene Sodium ◽  
External Potassium ◽  
Frog Sartorius

The pH difference across the cell membrane of frog sartorius muscle cells was measured with the distribution of 5,5-dimethyl-2,4-oxazolidine-dione (DMO) as the marker. Depolarization of the muscles to values at or below the contraction threshold caused by elevating external potassium up to approximately 20 mM resulted in an internal alkalinization. The change was smaller with superthreshold depolarization (20--30 mM [K+]). The alkalinization was blocked by agents that block calcium release from the sarcoplasmic reticulum (procaine and dantrolene sodium). Other agents that cause calcium release (caffeine, theophylline, and quinine) were found to give alkalinization when tested at concentrations just below the contracture threshold. Increased acidification of the extracellular medium was associated with the internal alkalinization. The data were interpreted as indicating the presence of a calcium-stimulated H+ and/or OH- ion transport system in the muscle membrane.

Carbohydrate requirement of immature nerve fibers during repetitive stimulation

1962 ◽  
Vol 202 (5) ◽  
pp. 845-848
Author(s):  
F. G. Carpenter
Keyword(s):  
Oxygen Consumption ◽  
Oxygen Uptake ◽  
Glucose Utilization ◽  
Compound Action Potential ◽  
Nerve Fibers ◽  
Repetitive Stimulation ◽  
Temperature Dependent ◽  
Ringer’S Solution ◽  
Sciatic Nerves ◽  
Compound Action

The compound action potential recorded from chick sciatic nerves was measured at 25 C during 10/sec stimulation after suspension in Ringer's solution containing 3, 10, and 20 mm glucose. Nerves sustained 90–100 min of 10/sec responses after 3 mm glucose, 200–285 min after 10 mm glucose, and 420–510 min after 20 mm glucose. Control responses from paired resting nerves diminished less than 5%. When the nerves no longer conducted impulses (blockade), a 2-hr suspension in 5 mm glucose restored the spike to 90%. Glucose utilization and oxygen consumption by the resting nerves are temperature dependent, but conduction survival is not. Oxygen uptake and glucose disappearance from the nerves were not accelerated by stimulation. Nerves in substrate-free Ringer's which block after 2 hr contain less than 5 µ glucose/ 100 mg nerve, but their oxygen consumption is unchanged. After blockade the nerves studied at 25 C contained 10–25 µ glucose, while at 35 C less than 5 µ was recovered.

scholarly journals The Effect of Caffeine on Radiocalcium Movement in Frog Sartorius

1961 ◽  
Vol 44 (5) ◽  
pp. 845-858 ◽  
Author(s):  
C. P. Bianchi
Keyword(s):  
Calcium Influx ◽  
Muscle Fibers ◽  
Thermodynamic Activity ◽  
Potassium Depolarization ◽  
Ionic Activity ◽  
Contractile State ◽  
Ringer’S Solution ◽  
Frog Sartorius

Caffeine increases resting calcium influx approximately threefold in normally polarized and in potassium-depolarized fibers of frog sartorius muscles. It does not affect the transient rapid increase in calcium influx that occurs at the beginning of a potassium depolarization. Calcium outflux in Ringer's solution, in zero calcium Ringer's solution, and in zero calcium Ringer's solution plus 0.004 M EDTA is also markedly increased by caffeine. The increased outflux reaches a rate which is approximately the same as the increased calcium influx. One interpretation of the findings is that caffeine reduces the binding of calcium both in the membrane and in the myoplasm; this increases the "permeability" to calcium and the ionic activity of calcium in muscle. This interpretation is consistent with the view that the contractile state of muscle is dependent at least in part on the thermodynamic activity of calcium in the muscle fibers.

Oxygen Consumption of Skeletal Muscles Relaxing Under Tension

1973 ◽  
Vol 51 (12) ◽  
pp. 981-985
Author(s):  
Roger McCarter
Keyword(s):  
Oxygen Consumption ◽  
Oxygen Uptake ◽  
External Load ◽  
Skeletal Muscles ◽  
Isometric Contractions ◽  
Moist Chamber ◽  
Series Of Experiments ◽  
Frog Sartorius

The oxygen consumption of isolated frog sartorius muscles was measured in a moist chamber at 12 °C. The muscles were indirectly stimulated to perform two series of contractions: in the first of these the imposed loads re-extended the muscles in relaxation, whereas in the second series relaxation occurred without the external load. No difference in oxygen consumption was detected between the two series of experiments. In both, lightly loaded contractions resulted in less oxygen uptake than isometric contractions at the resting length and heavily loaded contractions resulted in a greater consumption than in the isometric case. It is concluded that, within the limitations of the technique used, there is no additional oxygen consumption associated with the persistence of tension in a muscle during relaxation.

EFFECTS OF ADRENALECTOMY ON DAILY RHYTHMS IN OXYGEN CONSUMPTION OF TISSUES IN THE GROWING RAT

1971 ◽  
Vol 51 (1) ◽  
pp. 203-208
Author(s):  
J. GOULDING ◽  
D. BELLAMY
Keyword(s):  
Skeletal Muscle ◽  
Oxygen Consumption ◽  
Oxygen Uptake ◽  
Cardiac Muscle ◽  
Sex Difference ◽  
Male Rats ◽  
Tissue Oxygen ◽  
Daily Rhythms ◽  
Growing Rat

SUMMARY Daily rhythms in oxygen consumption were demonstrated in liver and skeletal muscle of male rats. Rhythms were observed only in skeletal muscle of females and in some cases there appeared to be a sex difference in the overall level of respiration. Respiration rhythms were not found in cardiac muscle of either sex. The effect of adrenalectomy upon the rhythms was examined. This indicated that the adrenal does not determine the phasing of the rhythms but does control the basal level of tissue oxygen uptake.

THE POTASSIUM UPTAKE AND RATE OF OXYGEN CONSUMPTION OF ISOLATED FROG SKELETAL MUSCLE IN THE PRESENCE OF INSULIN AND LACTATE

1956 ◽  
Vol 34 (5) ◽  
pp. 893-902 ◽  
Author(s):  
J. F. Manery ◽  
D. R. H. Gourley ◽  
Kenneth C. Fisher
Keyword(s):  
Skeletal Muscle ◽  
Oxygen Consumption ◽  
Concentration Gradient ◽  
Skeletal Muscles ◽  
Potassium Uptake ◽  
Frog Skeletal Muscle ◽  
High Concentration ◽  
Ringer’S Solution ◽  
Rate Of Oxygen Consumption

Insulin (5.0 × 10−2 units per ml.) and lactate (5.0 × 10−3 M) caused an uptake of potassium by intact frog skeletal muscles in vitro, and a concomitant loss of potassium from the medium surrounding the muscles; this fluid was Ringer's solution containing 2.1 meq. of potassium per liter. Thus a movement of potassium against a high concentration gradient has been demonstrated. In general, the conditions under which potassium uptake occurred stimulated the oxygen consumption of the muscles. Monoiodoacetic acid (2.5 × 10−4 M) abolished the effect of insulin on the potassium uptake and greatly reduced its effect on the oxygen consumption.

Respiration of Arctic Char (Salvelinus alpinus) From a High Arctic Lake

1973 ◽  
Vol 30 (6) ◽  
pp. 717-723 ◽  
Author(s):  
G. F. Holeton
Keyword(s):  
Oxygen Consumption ◽  
Oxygen Uptake ◽  
Cold Adaptation ◽  
Metabolic Response ◽  
Salvelinus Alpinus ◽  
High Arctic ◽  
Arctic Char ◽  
Short Term ◽  
Arctic Lake ◽  
Wet Weight

The routine of resting oxygen consumption of Arctic char (Salvelinus alpinus) from a high arctic lake (74°42′N) was measured at 2 (acclimated) and 6 C (unacclimated). The oxygen uptake versus wet weight relation at 2 C was: Log O2 uptake = 0.7316 Log weight – 1.0944. Oxygen uptake was low, not showing any evidence of "cold adaptation," and was comparable to projections of oxygen uptake versus temperature relations of other salmonid fish from lower latitudes.The short term metabolic response to a rise in temperature of 4 C was independent of body size except with fish with yolk sacs and weighing less than 0.125 g.

THE POTASSIUM UPTAKE AND RATE OF OXYGEN CONSUMPTION OF ISOLATED FROG SKELETAL MUSCLE IN THE PRESENCE OF INSULIN AND LACTATE

1956 ◽  
Vol 34 (1) ◽  
pp. 893-902 ◽  
Author(s):  
J. F. Manery ◽  
D. R. H. Gourley ◽  
Kenneth C. Fisher
Keyword(s):  
Skeletal Muscle ◽  
Oxygen Consumption ◽  
Concentration Gradient ◽  
Skeletal Muscles ◽  
Potassium Uptake ◽  
Frog Skeletal Muscle ◽  
High Concentration ◽  
Ringer’S Solution ◽  
Rate Of Oxygen Consumption

Insulin (5.0 × 10−2 units per ml.) and lactate (5.0 × 10−3 M) caused an uptake of potassium by intact frog skeletal muscles in vitro, and a concomitant loss of potassium from the medium surrounding the muscles; this fluid was Ringer's solution containing 2.1 meq. of potassium per liter. Thus a movement of potassium against a high concentration gradient has been demonstrated. In general, the conditions under which potassium uptake occurred stimulated the oxygen consumption of the muscles. Monoiodoacetic acid (2.5 × 10−4 M) abolished the effect of insulin on the potassium uptake and greatly reduced its effect on the oxygen consumption.

Thermogenesis after a mixed meal: role of leg and splanchnic tissues in men and women

1995 ◽  
Vol 268 (3) ◽  
pp. E433-E438 ◽  
Author(s):  
M. D. Jensen ◽  
C. M. Johnson ◽  
P. E. Cryer ◽  
M. J. Murray
Keyword(s):  
Skeletal Muscle ◽  
Oxygen Consumption ◽  
Oxygen Uptake ◽  
Muscle Metabolism ◽  
Mixed Meal ◽  
Relative Contribution ◽  
Energy Needs ◽  
Consumption Rates ◽  
Postprandial Thermogenesis

To determine the relative contribution of splanchnic and leg tissues to postprandial thermogenesis, systemic and regional oxygen consumption rates were measured in nine women and eight men before and for 6 h after the consumption of a mixed meal that provided one-third of the daily energy needs. In women, the increase in splanchnic oxygen uptake accounted for 63 +/- 12% of the postprandial increase in oxygen consumption, whereas in men it accounted for 35 +/- 14% (P = not significant between women and men). Leg oxygen uptake accounted for 11 +/- 4 and 10 +/- 3% of the increase in postprandial oxygen consumption in women and men, respectively. The combined data suggest that approximately 48% of postprandial thermogenesis over 6 h occurs in splanchnic tissues, whereas 30-35% occurs in skeletal muscle. Thus the increase in oxygen consumption after a mixed meal is primarily localized to splanchnic tissues, and major reductions in postprandial thermogenesis are unlikely to be attributable solely to abnormalities of skeletal muscle metabolism.

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