A SIMPLE METHOD FOR REARING PURE POPULATIONS OF THE FOLIAR NEMATODE, APHELENCHOIDES RITZEMABOSI, IN THE LABORATORY
Keyword(s):
A direct infestation method as a means of rearing pure populations of A phelenchoides ritzemabosi is described. A mature female is placed directly on the ventral surface of the leaf on a tiny droplet or a thin film of water. Reproduction takes place after successful entry of the nematode into the leaf tissue. The progeny of this female is then transferred to new leaves and the procedure is continued. Methods of maintaining high humidity around the leaves are described. The use of 'mist propagation frames' to enhance the spread of disease in greenhouse beds is recommended.