Total nitrogen, nonprotein nitrogen, and protein content in largemouth bass (Micropterus salmoides) with reference to quantitative protein estimates

1972 ◽  
Vol 50 (12) ◽  
pp. 1607-1610 ◽  
Author(s):  
A. J. Niimi
Keyword(s):  
Body Weight ◽  
Nitrogen Content ◽  
Protein Content ◽  
Total Nitrogen ◽  
Largemouth Bass ◽  
Micropterus Salmoides ◽  
Total Nitrogen Content ◽  
Nonprotein Nitrogen ◽  
Feeding Level

Nonprotein nitrogen (NPN) content in largemouth bass accounted for 9% of the total nitrogen content. This level was influenced by feeding level and probably body weight. A consideration for NPN is essential for quantitative protein estimates.

Direct and Indirect Determination of True Protein Content of Milk by Kjeldahl Analysis: Collaborative Study

1991 ◽  
Vol 74 (2) ◽  
pp. 281-288 ◽  
Author(s):  
David M Barbano ◽  
Joanna M Lynch ◽  
J Richard Fleming
Keyword(s):  
Nitrogen Content ◽  
Protein Content ◽  
Total Nitrogen ◽  
Collaborative Study ◽  
Total Nitrogen Content ◽  
Nonprotein Nitrogen ◽  
Protein Nitrogen ◽  
Nitrogen Determination ◽  
True Protein

Abstract Currently, the reference procedure for determination of the "protein" content of milk is based on measurement of the total nitrogen content of milk by the Kjeldahl method (AOAC method, 920.105). About 6% of the total nitrogen content of milk Is nonprotein nitrogen. Therefore, total nitrogen multiplied by the conversion factor 6.38 overestimates the true protein content of milk on average by about 6%. In the present study, new direct and Indirect methods were developed for measurement of the true protein content of whole milk by Kjeldahl nitrogen determination. Both new methods are sample preparation procedures used to fractionate the nitrogen-containing compounds In milk prior to measurement of the nitrogen content of these fractions by Kjeldahl analysis. The collaborative study consisted of 9 pairs of blind duplicate milk samples that were analyzed for total nitrogen, nonprotein nitrogen, and protein nitrogen by each of 10 laboratories. Both methods for true protein measurement (direct and Indirect) gave acceptable statistical performance characteristics and good agreement between methods. The new direct method requires about half the laboratory analysis work of the indirect method (i.e., total minus nonprotein nitrogen). The methods have been adopted official first action by AOAC as (1) a new method for nonprotein nitrogen determination in milk, (2) a new method (direct) for determination of protein nitrogen content of milk, and {3) an alternative method (indirect) for determination of protein nitrogen content of milk.

Bioenergetics and growth of largemouth bass (Micropterus salmoides) in relation to body weight and temperature

1974 ◽  
Vol 52 (4) ◽  
pp. 447-456 ◽  
Author(s):  
A. J. Niimi ◽  
F. W. H. Beamish
Keyword(s):  
Growth Rate ◽  
Body Composition ◽  
Body Weight ◽  
Moisture Content ◽  
Largemouth Bass ◽  
Micropterus Salmoides ◽  
Ash Content ◽  
Feeding Level ◽  
Growth Requirements ◽  
Wet Weight

Changes in growth and proximate body composition of largemouth bass (Micropterus salmoides) were examined for fish of 8–150 g, between 18 and 30C. For most weights, growth of fish (grams/day) was highest at 25C, and lowest in fish at 18C. This was attributable in part to a higher satiation (maximum) feeding level at 25C. For a fixed level of feeding, growth rate (percentage wet weight/day) was highest for fish held at 18C.Lipid content (percentage wet weight) increased with feeding level and was highest at 18C, Moisture content varied inversely with lipid. Protein and ash content did not vary appreciably with temperature, body weight, or feeding level.Maintenance requirement of bass, expressed as grams/day, was about twice that lost during food deprivation, but only slightly different when expressed as kilocalories/day.Energy requirements for nonfecal losses were estimated as 10% of intake energy at all feeding levels. Standard metabolism accounted for 50% of intake energy near maintenance, but only 10% at the satiation feeding level. Growth requirements increased From zero at maintenance to 40% of intake energy at satiation feeding.

scholarly journals Chemical composition of selected winter green pea (Pisum sativum L.) genotypes

2017 ◽  
Vol 82 (11) ◽  
pp. 1237-1246 ◽  
Author(s):  
Janko Cervenski ◽  
Dario Danojevic ◽  
Aleksandra Savic
Keyword(s):  
Nitrogen Content ◽  
Protein Content ◽  
Total Nitrogen ◽  
Oil Content ◽  
Starch Content ◽  
Ash Content ◽  
Total Nitrogen Content ◽  
Cellulose Content ◽  
Total Sugars ◽  
Fatty Oil

Breeding and selection of winter pea for seed quality is a serious challenge to every breeder. The result of breeding mainly depends on good knowledge of the genetic material. Chemical and technological analysis is necessary for an accurate determination of the following traits of technologically mature seed of the winter pea collection: protein content, total nitrogen content, total sugars content, starch content, fatty oil content, cellulose content, and ash content (g (100 g)-1). The protein content in the tested lines of pea was in the range 22.86?28.04 g (100 g)-1, the total nitrogen content 3.66?4.49 g (100 g)-1, total sugars content 10.30?14.67 g (100 g)-1, starch content 39.44?46.23 g (100 g)-1, fatty oil content 1.48?1.89 g (100 g)-1, cellulose content 8.79?10.28 g (100 g)-1 and ash content 3.08?3.67 g (100 g)-1. PCA analysis was used to identify the three components that collectively explained 81.59 % of the total variation. The first component was mainly defined by the ash and the total nitrogen, protein and cellulose contents. The second one, independent from the first one, was mainly correlated to the fatty oil and starch contents, while the third was defined by the content of total sugars.

scholarly journals Researches Regarding the Influence of Organic and Mineral Fertilization on the Soil Parameters and Total Crude Protein Content of Forages from a Permanent Meadow by Cluster Analysis (Part 1)

1970 ◽  
Vol 66 (1) ◽  
Author(s):  
Monica HĂRMĂNESCU ◽  
Alexandru MOISUC
Keyword(s):  
Cluster Analysis ◽  
Nitrogen Content ◽  
Protein Content ◽  
Total Nitrogen ◽  
Crude Protein ◽  
Total Nitrogen Content ◽  
Mineral Fertilizers ◽  
Soil Parameters ◽  
Crude Protein Content ◽  
Total Crude Protein

This paper presents researches regarding the influence of organic (sheep manure) and NPK mineral fertilizers on the soil parameters and total crude protein content (%) of forages, harvested in the middle of May, 2008, from a permanent meadow situated near Grădinari, in Caraş-Severin district. The studied permanent meadow was organized in ten trials fertilized with different doses of NPK mineral fertilizers, organic (sheep manure) and organic-mineral fertilizers. For each trial was made five replications. The total nitrogen content (%) was determinated by Kjeldahl method, and total crude protein was calculated by multiplying total nitrogen content with 6.25. The soil pH was determinated in aqueous solution (ratio soil / water = 1/10) using potentiometric method. Mobile forms of potassium and phosphorus were determinated by Egner-Riehm-Domingo method, extracted from soil with an acetate-lactate solution buffered at pH= 4.6, at 1/5 soil-solution ratio. The statistical interpretation was performed with Multivariate Exploratory Techniques, Cluster Analysis, implemented in Statistica 6 software. Using Cluster Analysis is possible to show the influence of organic and mineral fertilizers on the soil parameters (pH, total nitrogen content, mobile form of P, and mobile form of K) and total crude protein content of forages from the permanent meadow from Gradinari (Caraş-Severin District) by grouping all ten experimental trials in three main groups.

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