Epidermal cell proliferation in the bottlenose dolphin (Tursiops truncatus)

Epidermal cell proliferation in four bottlenose dolphins, Tursiops truncatus, was studied using radioactively labelled thymidine. The epidermal proliferation index (PI) of 7.4 ± 0.6% is 1.3 to 1.9 times higher than that reported for terrestrial mammals. The size of the proliferative pool, a function of the ratio of basal to surface area of the epidermis, was found to be 13.1:1. These two factors combine to give a large proliferative capacity, which contributes to the unusual thickness of the epidermis of Tursiops.

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Wound Healing in the Bottlenose Dolphin (Tursiops truncatus)

Canadian Journal of Fisheries and Aquatic Sciences ◽

10.1139/f85-029 ◽

1985 ◽

Vol 42 (2) ◽

pp. 216-228 ◽

Cited By ~ 13

Author(s):

L J. Bruce-Allen ◽

J. R. Geraci

Keyword(s):

Wound Healing ◽

Bottlenose Dolphin ◽

Tursiops Truncatus ◽

Bottlenose Dolphins ◽

Epidermal Cells ◽

Basal Cells ◽

Epidermal Thickness ◽

Mixed Cell ◽

Terrestrial Mammals ◽

Sterile Seawater

Early stages of wound healing were characterized in three bottlenose dolphins (Tursiops truncatus). A total of six 10-cm-long, 2-mm-deep incisions into the dermis were infused with sterile seawater for 30 min. Biopsies representing all surfaces of the cuts were taken at 2, 6, and 12 h and 1, 2, 3, 7, and 10 d. Histologically, wounds from 6 through 72 h were characterized by a mixed cell infiltrate predominated by neutrophils, and extensive areas of intraepidermal vesicles. Migrating epidermal cells bridged the incisional gap by 2 d, while elevated mitotic activity of basal cells restored full epidermal thickness by day 7. Notable was the absence of a traditional scab; its purpose was served by a transformed barrier layer of epidermal cells and vesicles. As well, an abnormal intracellular distribution of melanosomes typified new epidermal cells. The sequence and timing of healing in Tursiops are similar to those reported in terrestrial mammals; differences in the appearance of healing are most likely due to adaptations to an aquatic lifestyle.

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Lgr6 is dispensable for epidermal cell proliferation and wound repair

Experimental Dermatology ◽

10.1111/exd.13187 ◽

2016 ◽

Vol 26 (2) ◽

pp. 105-107 ◽

Cited By ~ 2

Author(s):

Lichun Jiang ◽

Long Wang ◽

Champ P. Chen ◽

Mei-Qing Li ◽

Xin-Hua Liao

Keyword(s):

Cell Proliferation ◽

Epidermal Cell ◽

Wound Repair ◽

Epidermal Cell Proliferation

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Involution and Cystic Transformation of the Thymus in the Bottlenose Dolphin, Tursiops truncatus

Veterinary Pathology ◽

10.1177/030098589403100603 ◽

1994 ◽

Vol 31 (6) ◽

pp. 648-653 ◽

Cited By ~ 23

Author(s):

D. F. Cowan

Keyword(s):

Body Size ◽

Bottlenose Dolphin ◽

Tursiops Truncatus ◽

Squamous Epithelium ◽

Gulf Coast ◽

Bottlenose Dolphins ◽

Adult Life ◽

Lymphoid Cells ◽

Texas Gulf Coast ◽

Thymic Cysts

The thymus glands of 10 bottlenose dolphins, Tursiops truncatus, collected along the Texas Gulf coast were examined using standard histologic and immunocytochemical methods. The thymus gland of Tursiops persists into adult life, represented by medulla and progressively thinning cortex. A network of epithelial cells, including Hassal bodies, is demonstrable using polyclonal anti-cytokeratin antibody. The network condenses, with loss of lymphoid cells as involution progresses. Cysts arise within the condensed network. These cysts, found in eight of 10 animals, increase in number and size with increasing body size. Body size tends to reflect age. Thymic cysts typically have an irregular shape when small but tend to become spherical as they enlarge. Theey may be lined by squamous epithelium of variable thickness. Eventually, the cysts become macroscopic and filled with a colloidlike material and may largely replace the thymus, which may be identified by noncystic remnants adjacent to the cysts.

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Epidermal cell proliferation in the equine hoof wall

Equine Veterinary Journal ◽

10.2746/0425164044877198 ◽

2010 ◽

Vol 36 (3) ◽

pp. 236-241 ◽

Cited By ~ 29

Author(s):

M. Daradka ◽

C. C. Pollitt

Keyword(s):

Cell Proliferation ◽

Epidermal Cell ◽

Equine Hoof ◽

Epidermal Cell Proliferation

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Development and Validation of Monoclonal and Polyclonal Antibodies for the Detection of Immunoglobulin G of Bottlenose Dolphins (Tursiops Truncatus)

Journal of Veterinary Diagnostic Investigation ◽

10.1177/104063870701900502 ◽

2007 ◽

Vol 19 (5) ◽

pp. 465-470 ◽

Cited By ~ 12

Author(s):

Hendrik H. Nollens ◽

Linda G. Green ◽

Diane Duke ◽

Michael T. Walsh ◽

Beth Chittick ◽

...

Keyword(s):

Monoclonal Antibodies ◽

Polyclonal Antibody ◽

Immunoglobulin G ◽

Bottlenose Dolphin ◽

Tursiops Truncatus ◽

Bottlenose Dolphins ◽

Enzyme Linked Immunosorbent Assay ◽

Common Bottlenose Dolphin ◽

Circulating Antibodies ◽

The Common

Antibodies directed against species-specific immunoglobulin G (IgG) have a broad range of applications in serologic and immunologic research and in the development of clinical assays. Validated anti-IgG antibodies for marine mammal species are in short supply. The objective of this study was to produce and validate antibodies with specificity for IgG of the common bottlenose dolphin ( Tursiops truncatus). Bottlenose dolphin IgG was purified using protein G. Two mouse monoclonal antibodies and a rabbit polyclonal antibody were developed from mice and rabbits immunized with bottlenose dolphin IgG. The specificity of the monoclonal antibodies and the polyclonal antibody for bottlenose dolphin IgG was first verified by Western blot analysis and enzyme-linked immunosorbent assay (ELISA). For further validation, both monoclonal antibodies and the polyclonal antibody were incorporated in an indirect ELISA for the detection of the immune response of bottlenose dolphins to a vaccine antigen. Three bottlenose dolphins were immunized with a commercial Erysipelothrix rhusiopathiae vaccine, and serial blood samples were collected from all dolphins for measurement of levels of circulating antibodies. Seroconversion was observed in all 3 dolphins by use of both monoclonal antibodies and the polyclonal antibody. Circulating antibodies were detectable as early as 6 days after immunization in 1 dolphin. Peak antibody levels were detected 14 days after the immunization. The ability to detect seroconversion in all 3 immunized bottlenose dolphins firmly establishes the specificity of the monoclonal antibodies and the polyclonal antibody for IgG of the common bottlenose dolphin.

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