Selenium and glutathione peroxidase activity in mountain goats

1985 ◽  
Vol 63 (7) ◽  
pp. 1544-1547 ◽  
Author(s):  
Charles T. Robbins ◽  
Steven M. Parish ◽  
Barbara L. Robbins
Keyword(s):  
Glutathione Peroxidase ◽  
Linear Function ◽  
Peroxidase Activity ◽  
Selenium Deficiency ◽  
Selenium Supplementation ◽  
Glutathione Peroxidase Activity ◽  
Oreamnos Americanus ◽  
Domestic Species ◽  
Mountain Goats ◽  
Low Selenium

Blood glutathione peroxidase (GSH-Px) activity in mountain goats (Oreamnos americanus) is a linear function of blood selenium. GSH-Px activity per unit of selenium (Se) in mountain goats is approximately double that published for the domestic cow and horse. It is hypothesized that high GSH-Px activity per unit selenium in mountain goats reduces their dietary selenium requirement relative to the above domestic species and is an essential adaptation for occupying low-selenium environments. GSH-Px activity peaked 20–30 days after injections of 0.1 and 0.3 mg Se/kg. A higher dose of 0.5 mg Se/kg delayed and decreased the development of peak GSH-Px activity relative to the two lower injection groups. When acute pathology owing to a selenium deficiency is demonstrated in wildlife capture programs, passive selenium supplementation, such as with selenium-containing salt blocks, should begin weeks to months before capture to provide adequate time for GSH-Px development.

scholarly journals Oxidative stress assessment by glutathione peroxidase activity and glutathione levels in response to selenium supplementation in patients with Mucopolysaccharidosis I, II and VI

2019 ◽  
Vol 42 (1) ◽  
pp. 1-8 ◽  
Author(s):  
José Araújo de Oliveira-Silva ◽  
Joyce Umbelino Pinto Yamamoto ◽  
Renata Bernardes de Oliveira ◽  
Vaneisse Cristina Lima Monteiro ◽  
Beatriz Jurkiewcz Frangipani ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Selenium Supplementation ◽  
Glutathione Peroxidase Activity ◽  
Stress Assessment ◽  
Mucopolysaccharidosis I

Selenium deficiency, endurance exercise capacity, and antioxidant status in rats

1987 ◽  
Vol 63 (6) ◽  
pp. 2532-2535 ◽  
Author(s):  
J. K. Lang ◽  
K. Gohil ◽  
L. Packer ◽  
R. F. Burk
Keyword(s):  
Physical Exercise ◽  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Antioxidant Status ◽  
Acute Exercise ◽  
Selenium Deficiency ◽  
Endurance Capacity ◽  
Glutathione Peroxidase Activity ◽  
Total Glutathione ◽  
Toxic Reactions

Increased O2 metabolism imposed by physical exercise is likely to augment the production of active O2 species that have been shown to react with lipids, proteins, and DNA. Antioxidants and antioxidant enzymes, such as the selenium enzyme glutathione peroxidase, minimize or prevent such potentially toxic reactions. This study shows that selenium deficiency decreases glutathione peroxidase activity in liver and muscle (less than 80%, P less than 0.001), increases total glutathione in liver, muscle, and plasma (P less than 0.05) and increases muscle cytochrome oxidase activity, and ubiquinone content (P less than 0.05) but has no effect on endurance capacity. Exercise to exhaustion resulted in a significant (P less than 0.001) elevation of total and oxidized glutathione (GSSG) and a significant (P less than 0.05) decrease of vitamin E in plasma of control and selenium-deficient rats. Acute exercise also increased tissue GSSG levels in both control and selenium-deficient groups of rats. Hence, despite a large depletion of selenium-deficient glutathione peroxidase, pronounced oxidation of glutathione to GSSG can be produced by the increased oxidative metabolism during physical exercise. The results suggest that the residual glutathione peroxidase activity is sufficient to detoxify hydroperoxides in exercising selenium-deficient animals and to prevent the impairment of endurance capacity.

Response of rat selenoprotein P to selenium administration and fate of its selenium

1991 ◽  
Vol 261 (1) ◽  
pp. E26-E30 ◽  
Author(s):  
R. F. Burk ◽  
K. E. Hill ◽  
R. Read ◽  
T. Bellew
Keyword(s):  
Glutathione Peroxidase ◽  
Peroxidase Activity ◽  
Selenium Deficiency ◽  
Rat Plasma ◽  
Female Rats ◽  
Selenoprotein P ◽  
Glutathione Peroxidase Activity ◽  
Uptake Mechanism ◽  
Male And Female Rats ◽  
And Control

Selenoprotein P is a glycoprotein that contains greater than 60% of the selenium in rat plasma. Physiological experiments were undertaken to gain insight into selenoprotein P function. Selenium-deficient rats were injected with doses of selenium ranging from 25 to 200 micrograms/kg, and the appearance of selenoprotein P was compared with the appearance of glutathione peroxidase activity in plasma and in liver. Selenoprotein P concentration increased to 35% of control by 6 h, whereas glutathione peroxidase activity increased minimally or not at all. Moreover, in rats given 100 and 200 micrograms selenium/kg, selenoprotein P reached 75% of its concentration in control rats at 24 h, whereas glutathione peroxidase activity reached only 6% of control. Cycloheximide pretreatment blocked the appearance of selenoprotein P in response to selenium injection. Male and female rats had similar concentrations of selenoprotein P. Partially purified selenoprotein P and plasma glutathione peroxidase labeled with 75Se were administered intravenously to selenium-deficient and control rats. 75Se given as selenoprotein P disappeared more rapidly from plasma than did 75Se given as glutathione peroxidase. Selenium deficiency did not significantly affect 75Se disappearance from plasma. At 2 h, brain, but not other tissues, took up more 75Se in selenium-deficient rats than in control rats when 75Se was given as selenoprotein P. This suggests that brain has a specific uptake mechanism for selenium given in the form of selenoprotein P. These results demonstrate that several physiological properties distinguish selenoprotein P from glutathione peroxidase. However, they do not clearly indicate its function.

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