PIXE analysis of trace elements included in oral lichen planus-affected mucosa

2015 ◽  
Vol 25 (01n02) ◽  
pp. 85-92
Author(s):  
S. Iijima ◽  
Y. Sugiyama ◽  
N. Matsumoto ◽  
A. Kumagai ◽  
S. Ishibashi ◽  
...  
Keyword(s):  
Trace Elements ◽  
Lichen Planus ◽  
Oral Mucosa ◽  
Oral Lichen Planus ◽  
Essential Elements ◽  
Control Group ◽  
Elemental Analysis Data ◽  
Pixe Analysis ◽  
Oral Lichen ◽  
Pixe Method

The purpose of our research is to identify causative metals by using particle-induced X-ray emission (PIXE) method to directly analyze trace elements in the oral mucosa with oral lichen planus (OLP). Subjects were 72 patients with OLP, and the patients are 25 men and 47 women, with a mean age of 60.1 years (OLP group). The control is elemental analysis data by PIXE method of 100 cases of the oral mucosa of healthy persons (control group). Seventeen essential elements and 12 contaminating elements were detected from the oral mucosa of the OLP and control groups. As to appearance ratio of elements in oral mucosa, among the contaminating elements, levels of Al, Ga, Sb, Hg and Pb were significantly lower, whereas Au and Y were significantly higher in the OLP group than in the control group. About concentration of elements in oral mucosa, the contaminating elements in the oral mucosa of the OLP group had a lower appearance ratio than in the control but each of the contaminating elements was more abundant. Among 25 subjects in the OLP group and seven in the control group, serum, oral mucosa and saliva were collected from the same individual. Abundance in serum and saliva varied depending on the element, but in general, a tendency was seen for contaminating elements to be more abundant in saliva than in serum and for essential elements to be more abundant in serum than in saliva.

TRACE ELEMENT ANALYSIS OF THE ORAL MUCOSA OF 22 PATIENTS WITH ORAL LICHEN PLANUS BY THE PIXE METHOD

2002 ◽  
Vol 12 (03n04) ◽  
pp. 159-166 ◽  
Author(s):  
Y. SUGIYAMA ◽  
S. ISHIBASHI ◽  
S. SEKIYAMA ◽  
K. SERA ◽  
S. FUTATSUGAWA
Keyword(s):  
Trace Elements ◽  
Oral Mucosa ◽  
Oral Lichen Planus ◽  
The Other ◽  
Control Group ◽  
Mean Values ◽  
The Mean ◽  
Oral Lichen ◽  
Pixe Method ◽  
Healthy Persons

We made a comparative study of the trace elements in the oral mucosa of 22 patients with oral lichen planus (OLP) and of 100 healthy persons by the PIXE method. Samples were excised from the oral mucosa of the OLP patients (OLP group) and from the buccal mucosa of healthy persons (control group). The results were as follows: Seventeen trace elements and 11 contaminating elements were detected in those oral mucosae. Al , Ti and Pb , which are contaminating elements, were detected in more than 65% of samples from both groups. The mean values of Se and Rb were significantly lower in the OLP group than in the control group. The mean values of the other elements did not differ significantly between the groups. As for the females over 30 years old, the OLP group showed significantly higher values of Zn and Sr than the control group. Their mean values of the other essential and contaminating elements did not differ significantly between the groups, although there was a trend toward higher values in the OLP group. We found by the PIXE method that Zn , Fe and Pd , which are d-transitional elements considered to cause allergic reaction, showed higher values in the oral mucosae of the OLP patients than in those of the healthy persons.

scholarly journals Evaluation of Cellular Proliferative Activity in Patients with Oral Lichen Planus and Hepatitis C through AgNOR Method

2014 ◽  
Vol 25 (6) ◽  
pp. 461-465
Author(s):  
João Paulo De Carli ◽  
Soluete Oliveira da Silva ◽  
Maria Salete Sandini Linden ◽  
Carmen Silvia Busin ◽  
Luiz Renato Paranhos ◽  
...  
Keyword(s):  
Hepatitis C ◽  
Lichen Planus ◽  
Oral Mucosa ◽  
Oral Lichen Planus ◽  
Clinical Signs ◽  
Hcv Infection ◽  
Control Group ◽  
Proliferative Potential ◽  
Group 2 ◽  
Oral Lichen

The objective of this study was to evaluate the cellular proliferative potential of oral lichen planus (OLP) lesions from patients without hepatitis C virus (HCV) by means of AgNOR method, as well as the cellular proliferative potential of the normal oral mucosa from patients with HCV, treated or untreated by interferon and ribavirin. A cross-sectional study was developed to investigate four groups: 10 HCV+ patients without clinical signs of OLP who had never been treated for HCV infection - Group 1; 10 HCV+ patients that were under interferon and ribavirin treatment - Group 2; 15 patients with reticular OLP lesions histopathologically confirmed, without HCV - Group 3; and 15 blood donors without HCV infection and no clinical signs of OLP GROUP 4 Control Group. The cytological material of all groups was collected by the liquid-based cytology technique. Then, the sedimented material from each patient was filled with the Nucleolar Organizer Regions impregnation by silver method (AgNOR). The count of NORs was performed on 100 epithelial cell nuclei per patient using the Image Tool(tm) software. The Tukey HSD test was used to compare the median value of NORs among the groups and showed that the oral mucosa of HCV+ patients previously treated with anti-HCV drugs (GROUP 2), presented a higher average number of NORs in relation to others (p<0.05). The anti-HCV treatment may be related to increased cell proliferation of oral mucosa, indicating a possible relationship between OLP and HCV+ patients treated with interferon and ribavirin.

scholarly journals Status of the permeability of hematosalivary barrier in oral mucosa with lichen planus

2016 ◽  
Vol 12 (1) ◽  
pp. 11-18
Author(s):  
С. Чуйкин ◽  
S. Chuykin ◽  
Г. Акмалова ◽  
G. Akmalova ◽  
А. Гильманов ◽  
...  
Keyword(s):  
Blood Serum ◽  
Lichen Planus ◽  
Oral Mucosa ◽  
Oral Lichen Planus ◽  
Oral Fluid ◽  
Clinical Course ◽  
Atomic Absorption Spectrophotometry ◽  
Mineral Elements ◽  
Control Group ◽  
Oral Lichen

The aim of the work was to study the permeability of hematosalivary barrier for mineral elements and the assessment of its role in the pathogenesis and clinical course of lichen planus of the oral mucosa. A total of 231 people were surveyed, which are divided into 6 groups depending on the clinical form of the disease and a control group. The concentrations of the mineral elements (zinc, copper and magnesium) in the blood serum and oral liquid are identified by atomic-absorption spectrophotometry. For each mineral element calculated coefficient ratio to reflects the operation of the hematosalivary barrier, which is the ratio of the concentration in the blood serum and oral fluid. Patients with oral lichen planus were found a violation of the permeability of hematosalivary barrier for some mineral elements, which is important in the pathogenesis of the disease. It appears as opposite changes of the mineral composition of blood serum and oral fluid, which have correlation with the severity of the clinical course of the disease. With increasing severity of the clinical course observed a significant decreasing of zinc, copper and a statistically significant increasing of magnesium in blood serum and oral fluid, which aggravates the severity of the clinical course of the oral lichen planus.

Salivary mir-27b Expression in Oral Lichen Planus Patients: A Series of Cases and a Narrative Review of Literature

2020 ◽  
Vol 19 (31) ◽  
pp. 2816-2823 ◽  
Author(s):  
Dario Di Stasio ◽  
Laura Mosca ◽  
Alberta Lucchese ◽  
Donatella Delle Cave ◽  
Hiromichi Kawasaki ◽  
...  
Keyword(s):  
Lichen Planus ◽  
Oral Lichen Planus ◽  
Inflammatory Diseases ◽  
Biological Fluids ◽  
Critical Role ◽  
Invasive Procedure ◽  
Control Group ◽  
Study Group ◽  
Immune Functions ◽  
Oral Lichen

Background: microRNAs play a critical role in auto-immunity, cell proliferation, differentiation and cell death. miRNAs are present in all biological fluids, and their expression is essential in maintaining regular immune functions and preventing autoimmunity, whereas miRNA dysregulation may be associated with the pathogenesis of autoimmune and inflammatory diseases. Oral lichen planus (OLP) is an inflammatory disease mediated by cytotoxic T cells attack against epithelial cells. The present study aims to perform a specific microRNA expression profile through the analysis of saliva in this disease. Methods: The study group was formed by five patients (mean age 62.8±1.98 years; 3 females/2 males) affected by oral lichen planus and control group by five healthy subjects (mean age 59.8 years±2.3; 3 females/ 2 males); using a low-density microarray analysis, we recorded a total of 98 differentially expressed miRNAs in the saliva of patients with oral lichen planus compared to the control group. The validation was performed for miR-27b with qRT-PCR in all saliva samples of oral lichen planus group. Results: 89 miRNAs were up-regulated and nine down-regulated. In details, levels of miR-21, miR- 125b, miR-203 and miR15b were increased (p<0.001) in study group while levels of miR-27b were about 3.0-fold decreased compared to controls (p<0.001) of miR-27b expression in OLP saliva. QRTPCR validation confirmed the down regulation of miR-27b in all saliva samples. Conclusions: Collecting saliva samples is a non-invasive procedure and is well accepted by all patients. microRNAs can be readily isolated and identified and can represent useful biomarkers of OLP.

Criteria for assessing severity of oral lichen planus

MedAlliance ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 73-81
Keyword(s):  
Lichen Planus ◽  
Oral Mucosa ◽  
Severity Score ◽  
Oral Lichen Planus ◽  
Unknown Etiology ◽  
Altered Level ◽  
Doppler Flowmetry ◽  
Serum Free ◽  
Control Subjects ◽  
Oral Lichen

Introduction. Oral lichen planus (OLP) is an autoim- mune disease of unknown etiology, manifested in vari- ous clinical forms. A correlation between intensity of OLP local manifestations and severity of general disturbances predisposing to altered oral mucosa have been poorly investigated. The aim of our study was to identify criteria for assessing OLP severity score. Methods. OLP patients and control subjects (not OLP) matched by hepatobiliary disease and type IIa dyslipidemia were examined. Samples collected during the study were as- sessed by way clinical examination, gas chromatographic analysis of serum free fatty acids (FFA), oral fluid, serum and fecal microbial marker analysis by gas chromatogra- phy/mass spectrometry, laser Doppler flowmetry of the oral mucosa, immunohistochemistry staining with an- ti-CD68 monoclonal antibody specific to apolipoprotein B-100-bearing macrophages. Results. It was found that OLP patients significantly differed from control subjects in the three FFA levels (lauric acid, eicosopentaenoic/ lauric and eicosopentaenoic/linoleic ratios), displayed four pathologic factors matching the altered level of mi- crobial markers. In addition, OLP patients were found to differ in oral mucosa blood filling both inside and outside lesions paralleled with oxidative changes immediately in oral mucosa highlighted as elevated amount of apo- lipoprotein B-100-bearing CD68-positive macrophages. Conclusion. OLP severity score is an integrate parameter which reflects oxidative changes and alterations in oral mucosa microhemocirculation primarily in serum free fat- ty acid composition, altered oral and gut microbiota as well as intensity score of clinical picture.

scholarly journals Role of apoptosis in erosive and reticular oral lichen planus exhibiting variable epithelial thickness

2008 ◽  
Vol 19 (3) ◽  
pp. 179-185 ◽  
Author(s):  
Juliana M. Caldeira Brant ◽  
Anilton C. Vasconcelos ◽  
Luciana V. Rodrigues
Keyword(s):  
Lichen Planus ◽  
Oral Mucosa ◽  
Oral Lichen Planus ◽  
Chronic Inflammatory Disease ◽  
Mechanical Trauma ◽  
Epithelial Thickness ◽  
Healthy Mucosa ◽  
Toxic Agents ◽  
Oral Lichen

Oral lichen planus (OLP) is a chronic inflammatory disease with different clinical types. Reticular and erosive forms are the most common. Although the cause of OLP remains speculative, many findings suggest auto-immune involvement, mediated by T lymphocytes against the basal keratinocytes. Inflammation, mechanical trauma or toxic agents can affect the epithelial homeostasia. Increased apoptosis may cause a decrease in epithelial thickness reflecting in the activity of the lesion. The objective of this study was to evaluate the occurrence of apoptosis and epithelial thickness in reticular and erosive forms of OLP. 15 samples of OLP each type (reticular and erosive) plus 10 of healthy mucosa were collected and processed. After morphometry, the apoptotic index and epitelial thickness were obtained. TUNEL and M30 CytoDEATH immunohistochemical assay were used to validate the morphologic criteria used. Apoptosis in the erosive OLP was significantly more intense than in the reticular type and both forms of OLP presented more apoptosis than the healthy oral mucosa. Healthy oral mucosa was thicker than both OLP forms and thicker in OLP reticular form than in the erosive one. The clinical differences between reticular and erosive forms of OLP are related to variations in epithelial thickness and in intensity of apoptosis.

scholarly journals Familial oral lichen planus in a 3-year-old boy: a case report with eight years of follow-up

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Fang Wang ◽  
Ya-Qin Tan ◽  
Jing Zhang ◽  
Gang Zhou
Keyword(s):  
Lichen Planus ◽  
Oral Mucosa ◽  
Buccal Mucosa ◽  
Oral Lichen Planus ◽  
Familial Aggregation ◽  
Pediatric Population ◽  
Case Presentation ◽  
Oral Lichen

Abstract Background Oral lichen planus (OLP) is a chronic mucocutaneous disease characterized by adult predominance and a prolonged course. However, it is rare in the pediatric population with familial aggregation. Case presentation A 3-year-old boy presented with pain and irritation on the oral mucosa while contacting spicy food for 2 months. Oral examination showed widespread whitish reticular and papular lesions on the lips, the dorsum of the tongue, and bilateral buccal mucosa, with diffuse erosions covered with pseudomembrane on the buccal mucosa. The boy’s parents were examined to exhibit white reticular and plaque-like lesions on their oral mucosa. The three patients were clinically diagnosed as affected by OLP and histopathologically confirmed. The boy underwent topical treatment with recombinant bovine basic fibroblast growth factor (rb-bFGF) gel, and oral lesions gradually resolved and healed. Neither of his parents received treatment. During the subsequent follow-ups, none of three patients underwent any medical treatment. Fortunately, their lesions had almost faded over 8 years. Conclusions Our case emphasizes that pediatric OLP should be recorded with family history. Besides, long-term periodic follow-up is recommended in pediatric patients with OLP for monitoring any changes in lesions.

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