Salivary mir-27b Expression in Oral Lichen Planus Patients: A Series of Cases and a Narrative Review of Literature

Background: microRNAs play a critical role in auto-immunity, cell proliferation, differentiation and cell death. miRNAs are present in all biological fluids, and their expression is essential in maintaining regular immune functions and preventing autoimmunity, whereas miRNA dysregulation may be associated with the pathogenesis of autoimmune and inflammatory diseases. Oral lichen planus (OLP) is an inflammatory disease mediated by cytotoxic T cells attack against epithelial cells. The present study aims to perform a specific microRNA expression profile through the analysis of saliva in this disease. Methods: The study group was formed by five patients (mean age 62.8±1.98 years; 3 females/2 males) affected by oral lichen planus and control group by five healthy subjects (mean age 59.8 years±2.3; 3 females/ 2 males); using a low-density microarray analysis, we recorded a total of 98 differentially expressed miRNAs in the saliva of patients with oral lichen planus compared to the control group. The validation was performed for miR-27b with qRT-PCR in all saliva samples of oral lichen planus group. Results: 89 miRNAs were up-regulated and nine down-regulated. In details, levels of miR-21, miR- 125b, miR-203 and miR15b were increased (p<0.001) in study group while levels of miR-27b were about 3.0-fold decreased compared to controls (p<0.001) of miR-27b expression in OLP saliva. QRTPCR validation confirmed the down regulation of miR-27b in all saliva samples. Conclusions: Collecting saliva samples is a non-invasive procedure and is well accepted by all patients. microRNAs can be readily isolated and identified and can represent useful biomarkers of OLP.

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Oral Lichen Planus in Relation to Transaminase Levels and Hepatitis C Viral Antibodies Detection: A Clinical Study

World Journal of Dentistry ◽

10.5005/jp-journals-10015-1366 ◽

2016 ◽

Vol 7 (2) ◽

pp. 64-68

Author(s):

M Manjunath ◽

TA Deepak ◽

Sowmya Krishna ◽

Geetika Sobti ◽

AG Annaji

Keyword(s):

Liver Disease ◽

Hepatitis C ◽

Clinical Study ◽

Lichen Planus ◽

Oral Lichen Planus ◽

Control Group ◽

Study Group ◽

Viral Antibodies ◽

Oral Lichen ◽

Antibodies Detection

ABSTRACT Background Lichen planus (LP) is a common long term inflammatory disorder that affects skin and mucous membranes. Though mostly asymptomatic in oral mucosa it may cause severe morbidity in erosive cases. The relation between LP and liver diseases especially hepatitis C have been proposed by many authors with varied results. Alteration in liver function tests reflected by increased levels of transaminase levels, i.e., serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT) with or without the presence of antibodies against hepatitis C virus (HCV) has been observed in oral lichen planus (OLP) patients in many studies. So this study was undertaken with the intent to further evaluate any alliance between OLP and liver disease principally hepatitis C. Materials and methods The study consisted of 30 histopathologically confirmed patients of OLP and 30 age and sex matched healthy volunteers visiting the outpatient department of a dental hospital over a period of 1 year. All subjects in both groups were subjected to SGOT, SGPT and HCV antibodies detection. Results The percentage of patients with elevated SGOT and SGPT values was higher among the OLP patients in the study group (40%) in comparison to subjects in the control group (7%). The mean values of SGOT and SGPT were also higher in the study group in comparison to the control group. No HCV antibodies were found in the serum of subjects in both the study group and control group. Conclusion Liver disease or hepatitis C can be considered a risk factor for LP and may lead to diagnosis, early treatment and possibly a better prognosis in asymptomatic OLP patients. Further studies with large samples are recommended to prove the current hypothesis regarding the association between LP and chronic liver disease and/or HCV infection. How to cite this article Sobti G, Manjunath M, Deepak TA, Krishna S, Annaji AG. Oral Lichen Planus in Relation to Transaminase Levels and Hepatitis C Viral Antibodies Detection: A Clinical Study. World J Dent 2016;7(2):64-68.

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Evaluation of Cellular Proliferative Activity in Patients with Oral Lichen Planus and Hepatitis C through AgNOR Method

Brazilian Dental Journal ◽

10.1590/0103-6440201302379 ◽

2014 ◽

Vol 25 (6) ◽

pp. 461-465

Author(s):

João Paulo De Carli ◽

Soluete Oliveira da Silva ◽

Maria Salete Sandini Linden ◽

Carmen Silvia Busin ◽

Luiz Renato Paranhos ◽

...

Keyword(s):

Hepatitis C ◽

Lichen Planus ◽

Oral Mucosa ◽

Oral Lichen Planus ◽

Clinical Signs ◽

Hcv Infection ◽

Control Group ◽

Proliferative Potential ◽

Group 2 ◽

Oral Lichen

The objective of this study was to evaluate the cellular proliferative potential of oral lichen planus (OLP) lesions from patients without hepatitis C virus (HCV) by means of AgNOR method, as well as the cellular proliferative potential of the normal oral mucosa from patients with HCV, treated or untreated by interferon and ribavirin. A cross-sectional study was developed to investigate four groups: 10 HCV+ patients without clinical signs of OLP who had never been treated for HCV infection - Group 1; 10 HCV+ patients that were under interferon and ribavirin treatment - Group 2; 15 patients with reticular OLP lesions histopathologically confirmed, without HCV - Group 3; and 15 blood donors without HCV infection and no clinical signs of OLP GROUP 4 Control Group. The cytological material of all groups was collected by the liquid-based cytology technique. Then, the sedimented material from each patient was filled with the Nucleolar Organizer Regions impregnation by silver method (AgNOR). The count of NORs was performed on 100 epithelial cell nuclei per patient using the Image Tool(tm) software. The Tukey HSD test was used to compare the median value of NORs among the groups and showed that the oral mucosa of HCV+ patients previously treated with anti-HCV drugs (GROUP 2), presented a higher average number of NORs in relation to others (p<0.05). The anti-HCV treatment may be related to increased cell proliferation of oral mucosa, indicating a possible relationship between OLP and HCV+ patients treated with interferon and ribavirin.

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Cytomorphometric analysis of exfoliated cells in oral lichen planus

CytoJournal ◽

10.4103/1742-6413.127214 ◽

2014 ◽

Vol 11 ◽

pp. 3 ◽

Cited By ~ 2

Author(s):

Chitturi Suryaprakash Ravi Teja ◽

A. Santha Devy ◽

R. Madhavan Nirmal ◽

P. M. Sunil ◽

M. Deepasree

Keyword(s):

Lichen Planus ◽

Oral Lichen Planus ◽

Control Group ◽

Exfoliative Cytology ◽

Normal Tissues ◽

Exfoliated Cells ◽

Healthy Mucosa ◽

Significant Difference ◽

Malignant Changes ◽

Oral Lichen

Background: Tumors are distinguished from normal tissues partly by their pronounced variability of cellular and nuclear dimensions. Therefore, such factors may be an indicator to assess whether the cells are malignant or not. Exfoliative cytology is a reliable tool in assessing such changes in the uterine cervix and has been used in the oral cavity also with success. The aims and objectives of the following study were to evaluate the malignant changes by assessing the quantitative parameters such as cytoplasmic diameter, cytoplasmic perimeter and cytoplasmic area (CD, CP, CA) and nuclear diameter, nuclear perimeter and nuclear area (ND, NP, NA) and cytoplasmic to nuclear ratio in the exfoliated cells of various subtypes of oral lichen planus (OLP) using cytomorphometry. Materials and Methods: Oral exfoliated cells from nineteen cases of histologically proven OLP (1 atrophic, 13 reticular, 4 erosive and 1 plaque) and ten controls with healthy mucosa were taken and stained by Feulgen-Rossenback reaction and cytomorphometric analysis was performed using an image analysis software. The parameters taken into account were CD, CP, CA and ND, NP, NA. Furthermore CA/NA was calculated. The parameters were statistically analyzed using the t-test. Results: Cytomorphometric analysis of all the parameters showed no significant difference between the control group and the reticular/plaque subtypes, whereas statistically significant (P < 0.05) differences was obtained between the control group and the atrophic/erosive subtypes group when compared using t-test. Conclusions: The cytomorphometric analysis of OLP shows that erosive/atrophic subtypes of OLP are at more risk and exfoliative cytology and cytomorphometry can be used as a tool to assess the malignant changes.

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Evaluation of Salivary Interleukin-12, Interleukin-23 Levels in Patients with Oral Lichen Planus and Squamous Cell Carcinoma

European Journal of General Dentistry ◽

10.1055/s-0041-1735765 ◽

2021 ◽

Vol 10 (02) ◽

pp. 91-95

Author(s):

Tahereh Nosratzehi ◽

Ebrahim Alijani ◽

Neda Hassanpoor

Keyword(s):

Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Lichen Planus ◽

Squamous Cell ◽

Oral Lichen Planus ◽

Inflammatory Diseases ◽

Interleukin 12 ◽

Enzyme Linked Immunosorbent Assay ◽

School Of Dentistry ◽

Oral Lichen

Abstract Objectives The present study aimed to evaluate interleukin-12 (IL-12) and IL-23 in the saliva of patients with oral lichen planus (OLP) and oral squamous cell carcinoma (SCC). Materials and Methods Thirty cases with clinical and histopathological OLP (bilateral lesions, papular and reticular lesions, and Wickham lines) (Group A), 30 with oral SCCs (OSCCs) (Group B), and 30 with no history of oral cancer, other lesions, or lichen planus (Group C) were enrolled at the Department of Oral Medicine School of Dentistry, Zahedan, Iran. The whole unstimulated saliva was collected and the salivary concentration of IL-12 and IL-23 was measured using an enzyme-linked immunosorbent assay in the laboratory. Statistical Analysis Data were analyzed using Kruskal–Wallis, Mann–Whitney tests, and Pearson’s correlation coefficients. Results In the present study, salivary IL-12 and IL-23 levels were higher in OSCC patients than in OLP and healthy individuals. Conclusions The results show that although IL-23 and IL-12 cytokines have an important role in the pathogenesis of chronic immunity and inflammatory diseases, further studies are required to assess matrix metalloproteinase links with tumor invasion.

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p53 as a Neoplastic Biomarker in Patients with Erosive and Plaque Like Forms of Oral Lichen Planus

The Journal of Contemporary Dental Practice ◽

10.5005/jp-journals-10024-1259 ◽

2013 ◽

Vol 14 (1) ◽

pp. 1-3 ◽

Cited By ~ 4

Author(s):

Iraj Mirzaii-Dizgah ◽

Farzaneh Agha-Hosseini

Keyword(s):

Clinical Significance ◽

Lichen Planus ◽

Oral Lichen Planus ◽

Control Group ◽

Whole Saliva ◽

The Mean ◽

Unstimulated Whole Saliva ◽

Oral Lichen ◽

Mean Concentration

ABSTRACT Aim Unstimulated whole salivary p53 was assessed in patients suffering from erosive and plaque-like form of oral lichen planus (OLP). Materials and methods Eighteen patients with erosive form, 17 patients suffering from plaque-like form and 38 noninvolvement subjects were enrolled. The unstimulated whole saliva p53 level was assayed by ELISA. Results The mean concentration of salivary p53 was significantly higher in patients with plaque-like form compared to both patients with erosive form and the control group. Conclusion We conclude that plaque like form of OLP is important in view of the potential for malignancy and is not safety form. Clinical significance It seems that all forms of OLP must be considered accurately, should be followed up with biannual examinations, and if possible, assessment of salivary p53 every year. How to cite this article Agha-Hosseini F, Mirzaii-Dizgah I. p53 as a Neoplastic Biomarker in Patients with Erosive and Plaque Like Forms of Oral Lichen Planus. J Contemp Dent Pract 2013;14(1):1-3.

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Assessment and Comparison of Salivary Survivin Biomarker in Oral Leukoplakia, Oral Lichen Planus, and Oral Cancer: A Comparative Study

World Journal of Dentistry ◽

10.5005/jp-journals-10015-1415 ◽

2017 ◽

Vol 8 (2) ◽

pp. 73-76 ◽

Cited By ~ 1

Author(s):

Ruchika Garg ◽

Arvind V Shetti ◽

Anjana S Bagewadi

Keyword(s):

Oral Cancer ◽

Comparative Study ◽

Malignant Transformation ◽

Lichen Planus ◽

Oral Lichen Planus ◽

Oral Leukoplakia ◽

Control Group ◽

Significant Difference ◽

Potentially Malignant ◽

Oral Lichen

ABSTRACT Introduction Oral squamous cell carcinoma (OSCC) is the most common malignant tumor of the oral cavity. It is preceded by various potentially malignant conditions like oral leukoplakia and oral lichen planus. Survivin is an inhibitor of apoptosis whose levels have been found increased in oral cancer as well as various potentially malignant conditions. Thus survivin can act as a biomarker and help in early detection of potentially malignant conditions which can prevent its transformation into malignancy. Aim To assess and compare the level of total human salivary survivin in oral leukoplakia, oral lichen planus, oral cancer, and control group. Materials and methods A total of 96 subjects were included in the study, which were further grouped into 24 in each group. The saliva was analyzed for survivin level among all the four groups. Survivin concentration (pg/mL) was studied in relation to clinical data. The results were analyzed using Mann—Whitney U test to derive the statistical difference. Results The average of levels of survivin in control group was 0.199 pg/mL, in oral leukoplakia group 0.312 pg/mL, in oral lichen planus group 0.380 pg/mL, and in oral cancer group 0.430 pg/mL. A comparison of all these groups revealed statistically significant difference among the groups. Conclusion Survivin may not be considered as an independent predictor of the malignant transformation for premalignant lesions but it can be an indicator for an increased risk of malignant transformation. How to cite this article Garg R, Shetti AV, Bagewadi AS. Assessment and Comparison of Salivary Survivin Biomarker in Oral Leukoplakia, Oral Lichen Planus, and Oral Cancer: A Comparative Study. World J Dent 2017;8(2):73-76.

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Improving the local treatment effect in oral lichen planus

Kazan medical journal ◽

10.17816/kmj1509 ◽

2014 ◽

Vol 95 (3) ◽

pp. 338-340

Author(s):

G I Ron ◽

G M Akmalova

Keyword(s):

Treatment Group ◽

Lichen Planus ◽

Oral Lichen Planus ◽

Local Treatment ◽

Main Group ◽

Aqua Complex ◽

Control Group ◽

Complex Treatment ◽

Local Use ◽

Oral Lichen

Aim. To assess the clinical efficacy of local use of titanium glycerosolvate aqua-complex (Tisolum) in combination with hyaluronidase as a component of the complex treatment of oral lichen planus. Methods. The study included 49 patients with typical form of oral lichen planus aged 29 to 65 years. The first group included 25 patients, who received the local treatment with glycerosolvate aqua-complex (Tisolum) in combination with hyaluronidase as a component of the complex treatment. The second group included 24 patients who were treated conventionally. Results. The patients of the treatment group reported the decrease of the oral mucosa tightness and roughness sensation 5.78±0.26 days after the treatment initiation, which was 1.5 times faster compared to the control group (8.6±0.34 days, р 0.05). On the seventh day of treatment, there was a significant papule deflorescence in 19 (76.0%) out of 25 patients of the main group, compared to 11 (45.8%) out of 24 patients of the control group (р 0.05). On the 10-th day, disappearance or downsizing of lesions without signs of active inflammation was observed in all 25 patients of the treatment group, compared to 16 (66.7%) of the control group patients (р 0.05). On the 14-th day, no mucosal lesions were observed in all 25 patients of the treatment group, which was significantly better compared to 9 (37.5%) out of 24 patients of the control group (р 0.05). Regression of clinical signs of the disease at the treatment end was registered in all patients of the main group and in 14 (58.3%) patients of the control group. Conclusion. Local use of composite gel containing titanium glycerosolvate aqua-complex (Tisolum) in combination with hyaluronidase as a component of the complex treatment of oral lichen planus is highly effective.

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Serum Vitamin Profile in Oral Lichen Planus Patients in Southwest of Iran

BioMed Research International ◽

10.1155/2021/8627435 ◽

2021 ◽

Vol 2021 ◽

pp. 1-7

Author(s):

Fahimeh Rezazadeh ◽

Sara Haghighat

Keyword(s):

Immune System ◽

Lichen Planus ◽

Oral Lichen Planus ◽

Serum Levels ◽

Control Group ◽

Related Disorder ◽

Significant Difference ◽

The Difference ◽

Southwest Of Iran ◽

Oral Lichen

Introduction. Oral lichen planus (OLP) is a chronic mucocutaneous disease. It is mainly an immune system-related disorder. Vitamins can modulate immune system functions, and thus, vitamin deficiency might have roles in exacerbating OLP. We aim to determine the serum levels of vitamins A, B12, C, D3, and E in OLP patients. Methods and Materials. 34 OLP patients referred to Shiraz Dental School entered the study. Blood samples were collected and levels of A, B12, C, D3, and E vitamins were measured in serum. 43 healthy people were also included as the control group. Serum levels of vitamins were measured by HPLC (A, B12, D3, and E) and Kiazist analyzing kit (vitamin C). Results. Most of the patients were female (62.3%), and the mean age of patients was 48.03 ± 11.57 . Serum levels of vitamins A, C, and E were lower in OLP patients in comparison with the healthy group; however, the difference was not significant. Vitamins B12 and D3 were higher in the OLP group but the difference was not significant. Conclusion. Serum levels of vitamins A, B12, C, D3, and E do not have a significant difference in OLP patients and healthy groups. These vitamins may not have a considerable role in OLP pathogenesis in the southwest of Iran.

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Association of Oral Lichen Planus with Dyslipidemia in Patients Of Rahim Yar Khan

10.53350/pjmhs2115113136 ◽

2021 ◽

Vol 15 (11) ◽

pp. 3136-3137

Author(s):

Nazia Hanif ◽

Tahir Hassan ◽

Muhammad Khurram Shahzad ◽

Raheel Tahir ◽

Madiha Anwar ◽

...

Keyword(s):

Total Cholesterol ◽

Lichen Planus ◽

Oral Lichen Planus ◽

Ldl Cholesterol ◽

Hdl Cholesterol ◽

Serum Total Cholesterol ◽

Control Group ◽

Chronic Inflammatory Condition ◽

The Mean ◽

Oral Lichen

Introduction: Oral lichen planus (LP) is a chronic inflammatory condition and this inflammation leads to alteration in lipid metabolism and increase the cardiovascular risk factors. Objective: To determine the prevalance of dyslipidemias in patient with oral lichen planus coming in dermatology outdoor of Sheikh Zayed Hoapital Rahim Yar Khan. Methods: It was a case control study that was conducted at Department of Dermatology, Sheikh Zayed Hospital Rahim Yar Khan during October 2016 to April 2017. In this study there were total 50 cases of oral LP and 50 controls of both genders with age range of 15 to 60 years. The cases of both the groups underwent fasting lipid profile in terms to look for serum total cholesterol, triglyceride, LDL cholesterol and HDL cholesterol. Results: In this study there were total 100 cases out of which 50 were cases of oral LP and 50 were controls. In cases groups there were 27 (54%) males and 23 (46%) females while in control group there were same number of males and females were taken with p= 0.85. The mean age in case group was 37.22±8.15 years while in control group was 35.66± 7.78 years while the mean BMI was 24.42±2.21 and 25.11±3.01 respectively. The mean difference in cases and controls in terms of serum total cholesterol, TG, HDL Cholesterol and LDL cholesterol was found statistically significant. Conclusion: Dyslipidemias are common in cases with oral lichen planus and all its parameters have significant association with it especially TG. Keywords; LP, Dyslipidemia, LDL, HDL, TG

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Evaluation of Uric Acid, Total Antioxidant and Lipid Peroxidation Parameters in Serum and Saliva of Patients with Oral Lichen Planus

Global Journal of Health Science ◽

10.5539/gjhs.v8n12p225 ◽

2016 ◽

Vol 8 (12) ◽

pp. 225 ◽

Cited By ~ 3

Author(s):

Atena Shiva ◽

Shahin Arab

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Lichen Planus ◽

Oral Lichen Planus ◽

Control Group ◽

Healthy Controls ◽

Total Antioxidant ◽

Healthy Control ◽

Oral Lichen ◽

And Oxidative Stress

BACKGROUND: Oral lichen planus (OLP) is a chronic and inflammation mucosal disease. Reactive Oxygen Species (ROS) and oxidative stress damage might be the cause. Malondialdehyd (MDA), one parameter of lipid peroxidation is appropriate for DNA damage in OLP disease.OBJECTIVE: To evaluate antioxidants and oxidative stress parameters in patients with oral lichen planus (OLP) diseases and healthy control group with compare on serum and salivary samples.MATERIAL & METHODS: The research population included 22 patients with OLP which recently diagnosed and 22 healthy controls matched for periodontal status. Total antioxidant activity (TAA), Malondialdehyde (MDA) or lipid peroxidation product and uric acid (UA) were evaluated in both serum and saliva. The t-tests were used for differences between the two groups in normal distributed variables and also Spearman’s rho correlation coefficient for assessing association between serum and salivary fluids.RESULTS: TAA levels in OLP patients showed significant result and lower than healthy control group (p=0.39). Also, results in the saliva MDA concentration, was significantly higher in OLP patients than controls. In correlation test, inverse and significant correlation was observed between the MDA and UA values(r=0. 682, P=0.0001) and a significant correlation was found between serum TAA and UA values.CONCLUSION: This study showed that OLP groups have higher cellular lipid peroxidation in compare to healthy controls and low level of TAA than controls. Patients with OLP are believed to be more at risk of antioxidant-oxidative stress imbalance.

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