Effect of Acupuncture Stimulation on Peripheral Nerve Regeneration Using Silicone Rubber Chambers

2001 ◽  
Vol 29 (03n04) ◽  
pp. 377-385 ◽  
Author(s):  
Yueh-Sheng Chen ◽  
Chun-Hsu Yao ◽  
Ter-Hsin Chen ◽  
Jaung-Geng Lin ◽  
Ching-Liang Hsieh ◽  
...  
Keyword(s):  
Blood Vessel ◽  
Silicone Rubber ◽  
Peripheral Nerves ◽  
Acupuncture Treatment ◽  
Peripheral Nerve Regeneration ◽  
Promoting Effect ◽  
Positive Effects ◽  
Vessel Area ◽  
Axon Density ◽  
Growth Promoting

The purpose of this study was to determine whether acupuncture could affect the regeneration of a 10-mn gap of rat sciatic nerve created between the proximal and distal nerve stumps, which were sutured into silicone rubber tubes. Empty silicone rubber tubes with no further treatment were used as controls. Six weeks after implantation, the animals received the acupuncture or the electroneedling treatment exhibited a more mature ultrastructural nerve organization with significantly higher numbers in the axon density, the blood vessel area, and the percentage of blood vessel area occupied in total nerve area than the controls. In addition, the electroneedling could combine both the needling and the electrical stimulation to potentiate the nerve-growth promoting effect of the acupuncture treatment. These results showed that acupuncture treatment could elicit positive effects on regenerated peripheral nerves.

Effects of Buyang Huanwu Decoction on Peripheral Nerve Regeneration Using Silicone Rubber Chambers

2001 ◽  
Vol 29 (03n04) ◽  
pp. 423-432 ◽  
Author(s):  
Yueh-Sheng Chen ◽  
Wen-Chiang Cheng ◽  
Chun-Hsu Yao ◽  
Ching-Liang Hsieh ◽  
Jaung-Geng Lin ◽  
...  
Keyword(s):  
Silicone Rubber ◽  
Peripheral Nerve Regeneration ◽  
Control Group ◽  
Ultrastructural Organization ◽  
Promoting Effect ◽  
Buyang Huanwu Decoction ◽  
Quantitative Histology ◽  
Qualitative And Quantitative ◽  
Growth Promoting ◽  
Distal Nerve

This study investigated the effect of Buyang Huanwu decoction on the regeneration of a 10-mm gap of rat sciatic nerve created between the proximal and distal nerve stumps, which were sutured into silicone rubber tubes. Empty silicone rubber tubes with no further treatment were used as controls. Six weeks after implantation, 89% of the animals orally administered the Buyang Huanwu decoction exhibited regeneration across the nerve gaps, whereas only 70% had regenerated in the control group. Both qualitative and quantitative histology of the regenerated nerves revealed a more mature ultrastructural organization with significantly higher numbers of myelinated axons, larger endoneurial areas, higher axon densities and a larger percentage of axon area per total nerve area in the Buyang Huanwu group than in the controls. These results showed that the Buyang Huanwu decoction had a growth-promoting effect on the regenerated nerves.

Earthworm Extracts Facilitate PC12 Cell Differentiation and Promote Axonal Sprouting in Peripheral Nerve Injury

2010 ◽  
Vol 38 (03) ◽  
pp. 547-560 ◽  
Author(s):  
Chao-Tsung Chen ◽  
Jaung-Geng Lin ◽  
Tung-Wu Lu ◽  
Fuu-Jen Tsai ◽  
Chih-Yang Huang ◽  
...  
Keyword(s):  
Peripheral Nerve ◽  
Peripheral Nerves ◽  
Peripheral Nerve Regeneration ◽  
In Vitro Study ◽  
Potential Growth ◽  
Marked Enhancement ◽  
Growth Promoting ◽  
Success Percentage

The present study provides in vitro and in vivo evaluations of earthworm (Pheretima aspergilum) on peripheral nerve regeneration. In the in vitro study, we found the earthworm (EW) water extracts caused a marked enhancement of the nerve growth factor-mediated neurite outgrowth from PC12 cells as well as the expressions of growth associated protein 43 and synapsin I. In the in vivo study, silicone rubber chambers filled with EW extracts were used to bridge a 10 mm sciatic nerve defect in rats. Eight weeks after implantation, the group receiving EW extracts had a much higher success percentage of regeneration (90%) compared to the control (60%) receiving the saline. In addition, quantitative histology of the successfully regenerated nerves revealed that myelinated axons in EW group at 31.25 μg/ml was significantly more than those in the controls (p < 0.05). These results showed that EW extracts can be a potential growth-promoting factor on regenerating peripheral nerves.

scholarly journals Electroacupuncture and Acupuncture Promote the Rat’s Transected Median Nerve Regeneration

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
C. Y. Ho ◽  
C. H. Yao ◽  
W. C. Chen ◽  
W. C. Shen ◽  
D. T. Bau
Keyword(s):  
Blood Vessel ◽  
Nerve Regeneration ◽  
Median Nerve ◽  
Silicone Rubber ◽  
Action Potentials ◽  
Muscle Action ◽  
Positive Effects ◽  
Injured Patients ◽  
Regeneration Processes ◽  
Made In

Background. Acupuncture and electroacupuncture treatments of damaged nerves may aid nerve regeneration related to hindlimb function, but the effects on the forelimb-related median nerve were not known.Methods. A gap was made in the median nerve of each rat by suturing the stumps into silicone rubber tubes. The influences of acupuncture and electroacupuncture treatments on transected median nerve regeneration were evaluated from morphological, electrophysiological, and functional angles.Results. Morphologically, the group receiving acupuncture and electroacupuncture treatments had larger total nerve area and blood vessel number compared with the controls. Electrophysiologically, the group receiving electroacupuncture had significantly larger amplitude and larger area of the evoked muscle action potentials compared with the controls. Functionally, the acupuncture and electroacupuncture treatments enhanced the injured paw’s ability to regain its grasping power and resulted in a faster efficiency to a new bilateral balance.Conclusion. Our findings provide multiapproach evidence of the efficacy of acupuncture and electroacupuncture treatments to the regeneration of median nerve. Indeed, acupuncture and electroacupuncture appear to have positive effects on the regeneration processes. This platform is beneficial to further study the clinical application of acupuncture and electroacupuncture alternative treatments on nerve-injured patients.

Xyloglucan- and cello-oligosaccharides: Antagonists of the growth-promoting effect of H+

1990 ◽  
Vol 80 (1) ◽  
pp. 109-113 ◽  
Author(s):  
Ester P. Lorences ◽  
Gordon J. McDougall ◽  
Stephen C. Fry
Keyword(s):  
Promoting Effect ◽  
Growth Promoting

Effects of follicular fluids on the growth of porcine preantral follicle and oocyte

Zygote ◽  
2008 ◽  
Vol 16 (3) ◽  
pp. 239-247 ◽  
Author(s):  
T. Metoki ◽  
H. Iwata ◽  
M. Itoh ◽  
M. Kasai ◽  
A. Takajyo ◽  
...  
Keyword(s):  
Calf Serum ◽  
Follicle Development ◽  
Preantral Follicle ◽  
Promoting Effect ◽  
Oocyte Growth ◽  
Preantral Follicles ◽  
Molecular Weights ◽  
Heat Treated ◽  
Growth Promoting ◽  
Follicular Fluids

SummaryWe examined the effect of supplementing the culture medium with follicular fluid (FF) on the growth of porcine preantral follicles and oocytes. Firstly, preantral follicles were retrieved from ovaries and then FF was collected from all antral follicles that were 2–7 mm in diameter (AFF), which included large follicles of 4–7 mm in diameter (LFF) and small follicles of 2–3 mm in diameter (SFF). When preantral follicles with a diameter of 250 μm were cultured in medium containing AFF, the growth of follicles and oocytes was greater than when follicles were cultured in medium containing fetal calf serum (FCS). When this growth-promoting effect in AFF was compared for LFF and SFF, the LFF were shown to be significantly more effective than SFF. This LFF effect was lost, however, when the concentration of LFF in the medium was decreased from 5% to 0.5% or when LFF were heat treated (60 °C for 30 min) or trypsin was added. In contrast, a decrease in SFF concentration from 5% to 0.5% and heat treatment of the SFF enhanced preantral follicle growth. Furthermore, proteins obtained from LFF that had molecular weights greater than 10 kDa (LFF > 10 kDa) had similar, but relatively reduced, growth-promoting properties. The remaining three LFF protein fractions (<10 kDa or <100 kDa or >100 kDa), however, did not have these growth-promoting properties. In conclusion, the supplementation of medium with LFF, rather than serum, enhanced preantral follicle and oocyte growth. Factors that enhanced follicle development in LFF and factors that suppressed follicle development in SFF were proteins and these LFF factors ranged in size from 10 kDa to over 100 kDa.

scholarly journals Cyr61 promotes Schwann cell proliferation and migration via αvβ3 integrin

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Zhenghui Cheng ◽  
Yawen Zhang ◽  
Yinchao Tian ◽  
Yuhan Chen ◽  
Fei Ding ◽  
...  
Keyword(s):  
Nerve Injury ◽  
Peripheral Nerves ◽  
Molecular Mechanisms ◽  
Αvβ3 Integrin ◽  
Promoting Effect ◽  
Ccn Family ◽  
And Migration ◽  
Proliferation And Migration

Abstract Background Schwann cells (SCs) play a crucial role in the repair of peripheral nerves. This is due to their ability to proliferate, migrate, and provide trophic support to axon regrowth. During peripheral nerve injury, SCs de-differentiate and reprogram to gain the ability to repair nerves. Cysteine-rich 61 (Cyr61/CCN1) is a member of the CCN family of matrix cell proteins and have been reported to be abundant in the secretome of repair mediating SCs. In this study we investigate the function of Cyr61 in SCs. Results We observed Cyr61 was expressed both in vivo and in vitro. The promoting effect of Cyr61 on SC proliferation and migration was through autocrine and paracrine mechanisms. SCs expressed αvβ3 integrin and the effect of Cyr61 on SC proliferation and migration could be blocked via αvβ3 integrin. Cyr61 could influence c-Jun protein expression in cultured SCs. Conclusions In this study, we found that Cyr61 promotes SC proliferation and migration via αvβ3 integrin and regulates c-Jun expression. Our study contributes to the understanding of cellular and molecular mechanisms underlying SC’s function during nerve injury, and thus, may facilitate the regeneration of peripheral nerves after injury.

The growth promoting effect of mammalian thioredoxin and the redox regulation of intestinal epithelial regeneration

2000 ◽  
Vol 118 (4) ◽  
pp. A602
Author(s):  
Shigeo Takaishi ◽  
Mitsutaka Sawada ◽  
Yukari Morita ◽  
Hiroaki Fukuzawa ◽  
Hiroshi Seno ◽  
...  
Keyword(s):  
Redox Regulation ◽  
Intestinal Epithelial ◽  
Promoting Effect ◽  
Epithelial Regeneration ◽  
Growth Promoting
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