Studies on Taiwan Folk Medicine, Thang-kau-tin (II): Measurement of Active Oxygen Scavenging Activity Using an ESR Technique

1995 ◽  
Vol 23 (01) ◽  
pp. 43-51 ◽  
Author(s):  
Jer-Min Lin ◽  
Chun-Ching Lin ◽  
Ming-Feng Chen ◽  
Takashi Ujiie ◽  
Atsushi Takada
Keyword(s):  
Hydroxyl Radical ◽  
Superoxide Radical ◽  
Folk Medicine ◽  
Radical Scavenger ◽  
Scavenging Activity ◽  
Oxygen Scavenging ◽  
Radical Scavenger Activity ◽  
Scavenger Activity ◽  
Spin Resonance ◽  
Mallotus Repandus

Using an electron spin resonance (ESR) technique, we measured the superoxide radical (• O 2-) and hydroxyl radical (•OH) scavenging activity from Mallotus repandus (Willd.) Muell.-Arg., Bauhinia championii Benth., Uncaria hirsuta Haviland and Uncaria rhynchophylla Miquel. • O 2- and •OH were detected as spin adducts of spin traps 5,5-dimethylpyrroline-N-oxide (DMPO). The scavenging potencies of water extracts of crude drugs were evaluated in terms of their ability to reduce the peaks of spin adducts. The extract of B. championii showed the greatest superoxide radical and hydroxyl radical scavenger activity.

Evaluation of the Antioxidant and Hepatoprotective Activity of Terminalia catappa

1997 ◽  
Vol 25 (02) ◽  
pp. 153-161 ◽  
Author(s):  
Chun-Ching Lin ◽  
Yea-Ling Chen ◽  
Jer Min Lin ◽  
Takashi Ujiie
Keyword(s):  
Rat Liver ◽  
Superoxide Radical ◽  
Radical Scavenging ◽  
Liver Homogenate ◽  
Hepatoprotective Activity ◽  
Radical Scavenger ◽  
Terminalia Catappa ◽  
Radical Scavenger Activity ◽  
Scavenger Activity ◽  
Antihepatotoxic Activity

The free radical scavenging and antihepatotoxic activity from Terminalia catappa was studied. Treatment with T. catappa water extracts showed antihepatotoxic activity against CCl 4-induced toxicity in the rat liver that was tested. The crude drug also exhibited anti-oxidant effects in FeCl 2-Ascorbic acid induced lipid peroxidation in the rat liver homogenate. Moreover, the superoxide radical scavenger effect of T. catappa was demonstrated using electron spin resonance (ESR) and spin-trapping technique. The results indicate that T. catappa possesses good antihepatotoxic activity and superoxide radical scavenger activity.

Anti-inflammatory and Radical Scavenge Effects of Arctium lappa

1996 ◽  
Vol 24 (02) ◽  
pp. 127-137 ◽  
Author(s):  
Chun-Ching Lin ◽  
Jer-Min Lin ◽  
Jeng-Jer Yang ◽  
Shu-Chuan Chuang ◽  
Takashi Ujiie
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Radical Scavenger ◽  
Scavenging Activity ◽  
Paw Edema ◽  
Arctium Lappa ◽  
Radical Scavenger Activity ◽  
Scavenger Activity

The effects of Arctium lappa L. (root) on anti-inflammatory and free radical scavenger activity were investigated. Subcutaneous administration of A. lappa crude extract significantly decreased carrageenan-induced rat paw edema. When simultaneously treated with CCl4, it produced pronounced activities against CCl4-induced acute liver damage. The free radical scavenging activity of its crude extract was also examined by means of an electron spin resonance (ESR) spectrometer. The IC50 of A. lappa extract on superoxide and hydroxyl radical scavenger activity was 2.06 mg/m 11.8 mg/m1, respectively. These findings suggest that Arctium lappa possess free radical scavenging activity. The inhibitory effects on carrageenan-induced paw edema and CCl4-induced hepatotoxicity could be due to the scavenging effect of A. lappa.

scholarly journals IDENTIFICATION AND ANTIOXIDANT ACTIVITY TEST OF SOME COMPOUNDS FROM METHANOL EXTRACT PEEL OF BANANA (Musa paradisiaca Linn.)

2010 ◽  
Vol 7 (1) ◽  
pp. 83-87 ◽  
Author(s):  
Sri Atun ◽  
Retno Arianingrum ◽  
Sri Handayani ◽  
Rudyansah Rudyansah ◽  
Mary Garson
Keyword(s):  
Hydroxyl Radical ◽  
Fenton Reaction ◽  
Radical Scavenger ◽  
Chloroform Fraction ◽  
1H And 13C Nmr ◽  
Musa Paradisiaca ◽  
Radical Scavenger Activity ◽  
Scavenger Activity ◽  
Hydroxyl Radical Scavenger ◽  
Measured Activity

The objective of these research was measured activity as antioxidant some compounds in methanol extracts of peel of banana (Musa paradisiaca Linn.), isolated some compounds which had activities as antioxidant, and determined this structure. Method of this study was extracted powdered peel of banana with methanol at room temperature. Extract was concentrated in vaccuo and then successively was partitioned with n-hexane, chloroform, etyl acetate, and buthanol. Antioxidant test from each fractions was measured by hydroxyl radical scavenger test with Fenton reaction method. The result of this study showed activity each fractions as  hydroxyl radical scavenger activity of chloroform, etyl acetate, and buthanol fraction were IC50 693.15; 2347.40; and 1071.14 mg/mL respectively. The isolation of secondary metabolite compounds from chloroform fraction obtained two isolate compounds. Identification by spectroscopy IR,  MS, 1H and 13C NMR one and two dimension showed that the compounds are 5,6,7,4'-tetrahidroxy-3,4-flavan-diol and a new compound cyclohexenon derivative (2-cyclohexene-1-on-2,4,4-trimethyl-3-O-2'-hydroxypropyl ether).   Keywords: antioxidant, peel of banana, Musa paradisiaca, hydroxyl radical scavenger

scholarly journals HPLC-DAD Polyphenolic Profiling and Antioxidant Activities of Sorghum bicolor during Germination

Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 417
Author(s):  
Ioana Otilia Ghinea ◽  
Maria Daniela Ionica Mihaila ◽  
Giorgiana-Valentina Blaga (Costea) ◽  
Sorin Marius Avramescu ◽  
Mihaela Cudalbeanu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Sorghum Bicolor ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Radical Scavenger ◽  
Ultrasound Assisted Extraction ◽  
Food Ingredient ◽  
Radical Scavenger Activity ◽  
Scavenger Activity ◽  
Sorghum Grains

The purpose of this study was to assess the suitability of the Romanian Albanus hybrid of Sorghum bicolor as a potential functional food ingredient. Ultrasound-assisted extraction in different solvents, together with spectrophotometric and chromatographic methods, was used to monitor the variation in total phenolic and flavonoid content and the antioxidant activity of raw sorghum grains before and during short germination periods (24, 36 and 48 h). The High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD) investigation of the extracts revealed that the ungerminated sorghum grains extracted with methanol had the highest diversity of phenolic compounds, while the total phenolic content (TPC) was the highest after 36 h of germination in both extract types: 1853 mg GAE/100 g for the methanolic extract and 1726 mg GAE/100 g for the ethanolic extract. The findings of this study showed that the TPC of sorghum extracts is strongly correlated with their antioxidant activity and, overall, that the studied extracts presented a good radical scavenger activity, which supports the benefits of alimentary uses of Sorghum bicolor grains.

scholarly journals Ocimum campechianum Mill. from Amazonian Ecuador: Chemical Composition and Biological Activities of Extracts and Their Main Constituents (Eugenol and Rosmarinic Acid)

Molecules ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 84
Author(s):  
Massimo Tacchini ◽  
Monica Paulina Echeverria Guevara ◽  
Alessandro Grandini ◽  
Immacolata Maresca ◽  
Matteo Radice ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Pseudomonas Syringae ◽  
Rosmarinic Acid ◽  
Plant Protection ◽  
Biological Activities ◽  
Radical Scavenger ◽  
Radical Scavenger Activity ◽  
Antioxidant Agents ◽  
Scavenger Activity

The essential oil (EO), the methanolic (MeOH), and the 70% ethanolic (70% EtOH) extracts obtained from the aerial parts of Ocimum campechianum Mill. (Ecuador) were chemically characterized through gas-chromatography coupled to mass spectrometry detector (GC-MS), high-performance liquid chromatography coupled to diode array-mass spectrometry detectors (HPLC-DAD-MS) and studied for their in vitro biological activity. The radical scavenger activity, performed by spectrophotometric 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays, highlighted significant IC50 values for the EO, extracts and their main constituents (eugenol and rosmarinic acid). EO (and eugenol) showed noteworthy activity against Pseudomonas syringae pv. syringae and a moderate effect against clinical Candida strains, with possible synergism in association to fluconazole against the latter microorganisms. The extracts and pure molecules exhibited weak cytotoxic activity against the HaCat cell line and no mutagenicity against Salmonella typhimurium TA98 and TA100 strains, giving indication of safety. Instead, EO showed a weak activity against adenocarcinomic human alveolar basal epithelial cells (A549). The above-mentioned evidence leads us to suggest a potential use of the crude drug, extracts, and EO in cosmetic formulation and food supplements as antioxidant agents. In addition, EO may also have a possible application in plant protection and anti-Candida formulations.

scholarly journals Gastroprotective Value of Berries: Evidences from Methanolic Extracts of Morus nigra and Rubus niveus Fruits

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Luciane Angela Nottar Nesello ◽  
Maria Luisa Maes Lima Beleza ◽  
Marihá Mariot ◽  
Luísa Nathália Bolda Mariano ◽  
Priscila de Souza ◽  
...  
Keyword(s):  
Atpase Activity ◽  
Free Radical Scavenger ◽  
Total Phenolic ◽  
Radical Scavenger ◽  
Lipid Hydroperoxides ◽  
Ulcer Area ◽  
Morus Nigra ◽  
Radical Scavenger Activity ◽  
Scavenger Activity

This study evaluated the gastroprotective value of the methanol extracts from fruits of Morus nigra L. (black mulberry (MEMN)) and Rubus niveus Thunb (raspberry (MERN)). The total phenolic compounds and flavonoids were measured, as well as the in vitro 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenger activity. The gastroprotective effects of the extracts against 60% ethanol/0.3 M HCl were evaluated in mice. After that, the lipid hydroperoxides and reduced glutathione levels at ulcerated tissue were determined. The effects of extracts on H+/K+-ATPase activity were also verified. The extracts exhibited high contents of polyphenols; however, MERN presented 1.5-fold higher levels. The presence of flavonoids also was confirmed. In addition, MEMN (IC50 = 13.74 μg/mL) and MERN (IC50 = 14.97 μg/mL) scavenged DPPH radical. The MEMN reduced the ulcer area only at 300 mg/kg (p.o.) by 64.06%. Interestingly, MERN decreased the ulcer area in a superior potency (ED50 = 20.88 mg/kg), reducing the ulcer area by 81.86% at 300 mg/kg, and increased the gastric mucin levels. The antioxidant effects of extracts were evidenced by reduced lipoperoxides and increased reduction of glutathione amount in the gastric mucosa. However, MEMN or MERN did not change the H+/K+-ATPase activity. These results confirm that M. nigra and R. niveus are berries with a gastroprotective value by strengthening of gastric protective factors.

Sesquiterpene lactones fromLychnophora pohlii: neutrophil chemiluminescence inhibition and free radical scavenger activity

2006 ◽  
Vol 58 (6) ◽  
pp. 853-858 ◽  
Author(s):  
Alexandre Kanashiro ◽  
Luciana M. Kabeya ◽  
Cristiane F. F. Grael ◽  
Christiane O. Jordão ◽  
Ana Elisa C.S. Azzolini ◽  
...  
Keyword(s):  
Free Radical ◽  
Sesquiterpene Lactones ◽  
Free Radical Scavenger ◽  
Radical Scavenger ◽  
Radical Scavenger Activity ◽  
Scavenger Activity

scholarly journals Effect of Nanoconfinement of Polyphenolic Extract from Grape Pomace into Functionalized Mesoporous Silica on Its Biocompatibility and Radical Scavenging Activity

Antioxidants ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 696 ◽  
Author(s):  
Ana-Maria Brezoiu ◽  
Laura Bajenaru ◽  
Daniela Berger ◽  
Raul-Augustin Mitran ◽  
Mihaela Deaconu ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Radical Scavenging ◽  
Phosphate Buffer Solution ◽  
Grape Pomace ◽  
Array Detector ◽  
Radical Scavenger ◽  
Radical Scavenger Activity ◽  
Polyphenolic Extract ◽  
Scavenger Activity ◽  
Mcm 41

The aim of this paper is to assess the properties of Mamaia (MM) grape pomace polyphenolic extract loaded onto pristine and functionalized MCM-41 mesoporous silica as potential ingredients for nutraceuticals or cosmetics. The chemical profile of hydroalcoholic polyphenolic extracts, prepared either by conventional extraction or microwave-assisted method, was analyzed by reverse-phase high-performance liquid chromatography with photodiode array detector (HPLC-PDA) analysis, while their radical scavenger activity (RSA) was evaluated using DPPH (2,2-diphenyl-1-picrylhydrazyl radical) and ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) assays. The extract-loaded materials were characterized by Fourier transform infrared (FTIR) spectroscopy, N2 adsorption-desorption isotherms, thermogravimetric analysis, as well as RSA (DPPH and ABTS assays). The polyphenols release profiles from pristine and functionalized (with mercaptopropyl, propyl sulfonic acid, cyanoethyl and propionic acid moieties) MCM-41-type supports were determined in phosphate buffer solution (PBS) pH 5.7. For selected materials containing embedded phytochemicals, cellular viability, and oxidative stress level on immortalized mouse embryonic fibroblast cell line (NIH3T3) were evaluated. A more acidic functional groups linked on silica pore walls determined a higher amount of phytochemicals released in PBS. The extract-loaded materials showed a good cytocompatibility on tested concentrations. The embedded extract preserved better the RSA over time than the free extract. The polyphenols-loaded MCM-41-type silica materials, especially MM@MCM-COOH material, demonstrated a good in vitro antioxidant effect on NIH3T3 cells, being potential candidates for nutraceutical or cosmetic formulations.

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