Evaluation of the Antioxidant and Hepatoprotective Activity of Terminalia catappa

1997 ◽  
Vol 25 (02) ◽  
pp. 153-161 ◽  
Author(s):  
Chun-Ching Lin ◽  
Yea-Ling Chen ◽  
Jer Min Lin ◽  
Takashi Ujiie
Keyword(s):  
Rat Liver ◽  
Superoxide Radical ◽  
Radical Scavenging ◽  
Liver Homogenate ◽  
Hepatoprotective Activity ◽  
Radical Scavenger ◽  
Terminalia Catappa ◽  
Radical Scavenger Activity ◽  
Scavenger Activity ◽  
Antihepatotoxic Activity

The free radical scavenging and antihepatotoxic activity from Terminalia catappa was studied. Treatment with T. catappa water extracts showed antihepatotoxic activity against CCl 4-induced toxicity in the rat liver that was tested. The crude drug also exhibited anti-oxidant effects in FeCl 2-Ascorbic acid induced lipid peroxidation in the rat liver homogenate. Moreover, the superoxide radical scavenger effect of T. catappa was demonstrated using electron spin resonance (ESR) and spin-trapping technique. The results indicate that T. catappa possesses good antihepatotoxic activity and superoxide radical scavenger activity.

scholarly journals Effect of Nanoconfinement of Polyphenolic Extract from Grape Pomace into Functionalized Mesoporous Silica on Its Biocompatibility and Radical Scavenging Activity

Antioxidants ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 696 ◽  
Author(s):  
Ana-Maria Brezoiu ◽  
Laura Bajenaru ◽  
Daniela Berger ◽  
Raul-Augustin Mitran ◽  
Mihaela Deaconu ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Radical Scavenging ◽  
Phosphate Buffer Solution ◽  
Grape Pomace ◽  
Array Detector ◽  
Radical Scavenger ◽  
Radical Scavenger Activity ◽  
Polyphenolic Extract ◽  
Scavenger Activity ◽  
Mcm 41

The aim of this paper is to assess the properties of Mamaia (MM) grape pomace polyphenolic extract loaded onto pristine and functionalized MCM-41 mesoporous silica as potential ingredients for nutraceuticals or cosmetics. The chemical profile of hydroalcoholic polyphenolic extracts, prepared either by conventional extraction or microwave-assisted method, was analyzed by reverse-phase high-performance liquid chromatography with photodiode array detector (HPLC-PDA) analysis, while their radical scavenger activity (RSA) was evaluated using DPPH (2,2-diphenyl-1-picrylhydrazyl radical) and ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) assays. The extract-loaded materials were characterized by Fourier transform infrared (FTIR) spectroscopy, N2 adsorption-desorption isotherms, thermogravimetric analysis, as well as RSA (DPPH and ABTS assays). The polyphenols release profiles from pristine and functionalized (with mercaptopropyl, propyl sulfonic acid, cyanoethyl and propionic acid moieties) MCM-41-type supports were determined in phosphate buffer solution (PBS) pH 5.7. For selected materials containing embedded phytochemicals, cellular viability, and oxidative stress level on immortalized mouse embryonic fibroblast cell line (NIH3T3) were evaluated. A more acidic functional groups linked on silica pore walls determined a higher amount of phytochemicals released in PBS. The extract-loaded materials showed a good cytocompatibility on tested concentrations. The embedded extract preserved better the RSA over time than the free extract. The polyphenols-loaded MCM-41-type silica materials, especially MM@MCM-COOH material, demonstrated a good in vitro antioxidant effect on NIH3T3 cells, being potential candidates for nutraceutical or cosmetic formulations.

scholarly journals Comparative Nutrient Element and Antioxidant Characterization of Berry Fruit Species and Cultivars Grown in Hungary

HortScience ◽  
2008 ◽  
Vol 43 (6) ◽  
pp. 1711-1715 ◽  
Author(s):  
Attila Hegedűs ◽  
Emőke Balogh ◽  
Rita Engel ◽  
Béla Zoltan Sipos ◽  
János Papp ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Radical Scavenging ◽  
Elemental Content ◽  
Total Phenol Content ◽  
Radical Scavenger ◽  
Black Currant ◽  
Antioxidant Power ◽  
Radical Scavenger Activity ◽  
Reducing Ability ◽  
Scavenger Activity

Fruits of four berry species (strawberry, raspberry, red and black currants) were compared in their elemental composition (Al, B, Ba, Ca, Cu, Fe, K, Mg, Mn, Na, P, and Zn) and redox parameters involving total phenol content (TPC), ferric reducing ability (FRAP), 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, and total radical scavenger activity (TRSA). Berry cultivars contained significantly higher (P ≤ 0.05) amounts of most detected elements as compared with apple with many maximum elemental values demonstrated by the black currant ‘Otelo’. Black currant also had the greatest antioxidant capacity as demonstrated through all conducted assays. The results obtained through FRAP, TPC, and TRSA assays were closely correlated, whereas TRSA and DPPH varied independently. Our study provides valuable information on the antioxidant capacity of several berry species grown in Hungary and highlights the crucial influence of cultivar on elemental content and antioxidant power of berry fruits. This study demonstrated the ability to select berry cultivars for specific nutritional purposes or assign parental lines in functional breeding programs.

scholarly journals Polyphenol Composition and Antioxidant Capacity of Three Lysimachia Species

2014 ◽  
Vol 9 (10) ◽  
pp. 1934578X1400901 ◽  
Author(s):  
Anita Toth ◽  
Gergo Toth ◽  
Agnes Kery
Keyword(s):  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Flavonoid Glycosides ◽  
Radical Scavenger ◽  
Polyphenol Content ◽  
Radical Scavenger Activity ◽  
External Standard Method ◽  
Scavenger Activity ◽  
Column Chromatographic

The polyphenol composition and antioxidant properties of three Lysimachia species ( L. nummularia L., L. vulgaris L. and L. punctata L.) and their column chromatographic fractions were investigated. The antioxidant activity of herb extracts and 54 different column chromatographic fractions was evaluated using in vitro DPPH• and ABTS•+ decolorization tests. The total polyphenol content was determined by spectrophotometric methods. The phenolic compounds of extracts of different Lysimachia species and their bioactive fractions were characterized by online chromatographic methods. For identification of the compounds, UV spectral data, accurate molecular mass and formula, as well as MS and fragmentation patterns given by LC-DAD-ESI/MS/MS and LC-ESI-TOF analyses were used. Quantification of the compounds was performed by LC-DAD using an external standard method. In the extracts, caffeic acid derivatives, chlorogenic acid, free flavonoid aglycones, and 11 various flavonoid glycosides were identified. Flavonoid composition of Lysimachia extracts showed significant differences. L. punctata extracts had the strongest DPPH• and ABTS•+ radical scavenger activity (IC50=43.3 μg/mL and 21.3 μg/mL), due to their high myricitrin and quercetin-hexoside content. Correlation between polyphenol content and radical scavenging activity of each column chromatographic fractions is also included.

scholarly journals Evaluation of free radical scavenging activity of leaf of Hypericum mysorense Heyne

2017 ◽  
Vol 9 (4) ◽  
pp. 589
Author(s):  
Sornalakshmi V ◽  
Tresina PS ◽  
Paulpriya K ◽  
Veerabahu Ramasamy Mohan
Keyword(s):  
Free Radical ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Ethanol Extract ◽  
Free Radical Scavenging ◽  
Model Systems ◽  
Radical Scavenger ◽  
Radical Scavenger Activity ◽  
Reducing Ability ◽  
Scavenger Activity

<em>Hypericum </em>is a well-known plant genus in herbal medicine. <em>Hypericum mysorense</em> is well known in folklore medicine for its varied therapeutic potential. The <em>in vitro </em>antioxidant activity of petroleum ether, benzene, ethyl acetate, methanol and ethanol extract of leaf of <em>Hypericum mysorense </em>have been tested using various antioxidant model systems viz; DPPH, hydroxyl, superoxide, ABTS and reducing power. The scavenging effect increases with the concentration of standard and samples. Among the solvent tested, ethanol extract exhibited highest DPPH (116.33% inhibition), hydroxyl (101.84%), superoxide (116.09%) and ABTS (119.22%) radical scavenger activity. Methanol extract of <em>H.mysorense </em>shows the highest reducing ability. The aim and purpose of this study is to show the significant free radical scavenging potential of <em>H.mysorense </em>leaf which can be experimented for the treatment of various free radical mediated ailments

Anti-inflammatory and Radical Scavenge Effects of Arctium lappa

1996 ◽  
Vol 24 (02) ◽  
pp. 127-137 ◽  
Author(s):  
Chun-Ching Lin ◽  
Jer-Min Lin ◽  
Jeng-Jer Yang ◽  
Shu-Chuan Chuang ◽  
Takashi Ujiie
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Radical Scavenger ◽  
Scavenging Activity ◽  
Paw Edema ◽  
Arctium Lappa ◽  
Radical Scavenger Activity ◽  
Scavenger Activity

The effects of Arctium lappa L. (root) on anti-inflammatory and free radical scavenger activity were investigated. Subcutaneous administration of A. lappa crude extract significantly decreased carrageenan-induced rat paw edema. When simultaneously treated with CCl4, it produced pronounced activities against CCl4-induced acute liver damage. The free radical scavenging activity of its crude extract was also examined by means of an electron spin resonance (ESR) spectrometer. The IC50 of A. lappa extract on superoxide and hydroxyl radical scavenger activity was 2.06 mg/m 11.8 mg/m1, respectively. These findings suggest that Arctium lappa possess free radical scavenging activity. The inhibitory effects on carrageenan-induced paw edema and CCl4-induced hepatotoxicity could be due to the scavenging effect of A. lappa.

Studies on Taiwan Folk Medicine, Thang-kau-tin (II): Measurement of Active Oxygen Scavenging Activity Using an ESR Technique

1995 ◽  
Vol 23 (01) ◽  
pp. 43-51 ◽  
Author(s):  
Jer-Min Lin ◽  
Chun-Ching Lin ◽  
Ming-Feng Chen ◽  
Takashi Ujiie ◽  
Atsushi Takada
Keyword(s):  
Hydroxyl Radical ◽  
Superoxide Radical ◽  
Folk Medicine ◽  
Radical Scavenger ◽  
Scavenging Activity ◽  
Oxygen Scavenging ◽  
Radical Scavenger Activity ◽  
Scavenger Activity ◽  
Spin Resonance ◽  
Mallotus Repandus

Using an electron spin resonance (ESR) technique, we measured the superoxide radical (• O 2-) and hydroxyl radical (•OH) scavenging activity from Mallotus repandus (Willd.) Muell.-Arg., Bauhinia championii Benth., Uncaria hirsuta Haviland and Uncaria rhynchophylla Miquel. • O 2- and •OH were detected as spin adducts of spin traps 5,5-dimethylpyrroline-N-oxide (DMPO). The scavenging potencies of water extracts of crude drugs were evaluated in terms of their ability to reduce the peaks of spin adducts. The extract of B. championii showed the greatest superoxide radical and hydroxyl radical scavenger activity.

scholarly journals Antioxidant-Free Radical Scavenging of Some Euphorbiaceae Herbs

2011 ◽  
Vol 2 (1) ◽  
pp. 146
Author(s):  
Titik Sunarni ◽  
Fransiska Leviana
Keyword(s):  
Acetic Acid ◽  
Free Radical ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenger ◽  
Radical Scavenger ◽  
Radical Scavenger Activity ◽  
Ic50 Value ◽  
Scavenger Activity ◽  
Acetic Acid Water

In order to screen natural antioxidant, the research about antioxidant of some Euphobiaceae herbs, have been conducted. The air-dried herbs  of Euphorbia heterophylla L, Phyllanthus acidus (L.) Skeels, and Phyllanthus buxifolius Muell Arg were extracted with metanol. The obtained extract was concentrated and then suspended to produce n-hexane, ethyl acetat and aqueous fractions. Free radical scavenger activity against DPPH (1,1-diphenyl-2-pycrylhydrazyl) measured by spectrophotometric method  and the IC50 value was determined. The compounds of  active fraction had been identified by TLC method. All of the herbs showed activity as DPPH scavenger. Among these herbs, Euphorbia heterophylla L. and Phyllanthus buxifolius Muell, Arg. exhibited a strong free radical scavenging of ethyl acetat fraction with IC50 value 5,88 µg/ml and 4,64 µg/ml. The result of TLC by mobile phase n-buthanol-acetic acid-water (4:1:5) and acetic acid 15% showed flavonoid compound.Keywords: Euphorbiaceae herbs, antioxidant, DPPH, flavonoid

scholarly journals HPLC-DAD Polyphenolic Profiling and Antioxidant Activities of Sorghum bicolor during Germination

Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 417
Author(s):  
Ioana Otilia Ghinea ◽  
Maria Daniela Ionica Mihaila ◽  
Giorgiana-Valentina Blaga (Costea) ◽  
Sorin Marius Avramescu ◽  
Mihaela Cudalbeanu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Sorghum Bicolor ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Radical Scavenger ◽  
Ultrasound Assisted Extraction ◽  
Food Ingredient ◽  
Radical Scavenger Activity ◽  
Scavenger Activity ◽  
Sorghum Grains

The purpose of this study was to assess the suitability of the Romanian Albanus hybrid of Sorghum bicolor as a potential functional food ingredient. Ultrasound-assisted extraction in different solvents, together with spectrophotometric and chromatographic methods, was used to monitor the variation in total phenolic and flavonoid content and the antioxidant activity of raw sorghum grains before and during short germination periods (24, 36 and 48 h). The High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD) investigation of the extracts revealed that the ungerminated sorghum grains extracted with methanol had the highest diversity of phenolic compounds, while the total phenolic content (TPC) was the highest after 36 h of germination in both extract types: 1853 mg GAE/100 g for the methanolic extract and 1726 mg GAE/100 g for the ethanolic extract. The findings of this study showed that the TPC of sorghum extracts is strongly correlated with their antioxidant activity and, overall, that the studied extracts presented a good radical scavenger activity, which supports the benefits of alimentary uses of Sorghum bicolor grains.

scholarly journals Hepatoprotective studies on methanolic extract fractions of Lindernia ciliata and development of qualitative analytical profile for the bioactive extract

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Praneetha Pallerla ◽  
Narsimha Reddy Yellu ◽  
Ravi Kumar Bobbala
Keyword(s):  
Methanolic Extract ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Liver Homogenate ◽  
Reducing Power ◽  
Hepatoprotective Activity ◽  
Total Phenolic ◽  
Effective Fraction

Abstract Background The objective of the study is to evaluate the hepatoprotective activity of methanolic extract fractions of Lindernia ciliata (LC) and development of qualitative analytical profile of the bioactive fraction using HPLC fingerprinting analysis. All the fractions of methanolic extract of Lindernia ciliata (LCME) are assessed for their total phenolic, flavonoid contents and in vitro antioxidant properties by using DPPH, superoxide, nitric oxide, hydroxyl radical scavenging activities and reducing power assay. Acute toxicity study was conducted for all the fractions and the two test doses 50 and 100 mg/kg were selected for the hepatoprotective study. Liver damage was induced in different groups of rats by administering 3 g/kg.b.w.p.o. paracetamol and the effect of fractions were tested for hepatoprotective potential by evaluating serum biochemical parameters and histology of liver of rats. The effective fraction was evaluated for its antihepatotoxic activity against D-Galactosamine (400 mg/kg b.w. i.p.) and in vivo antioxidant parameters viz., Glutathione (GSH), Melondialdehyde (MDA) and Catalase (CAT) levels are estimated using liver homogenate. Results Among all the fractions, butanone fraction of LCME, (BNF-LCME) has shown better hepatoprotective activity and hence it is selected to evaluate the antihepatotoxicity against D-GaIN. The activity of BNF-LCME is well supported in in vitro and in vivo antioxidant studies and may be attributed to flavonoidal, phenolic compounds present in the fraction. Hence, BNF-LCME was subjected to the development of qualitative analytical profile using HPLC finger printing analysis. Conclusions All the fractions of LCME exhibited significant hepatoprotective activity and BNF-LCME (50 mg/kg) was identified as the most effective fraction.

scholarly journals Ocimum campechianum Mill. from Amazonian Ecuador: Chemical Composition and Biological Activities of Extracts and Their Main Constituents (Eugenol and Rosmarinic Acid)

Molecules ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 84
Author(s):  
Massimo Tacchini ◽  
Monica Paulina Echeverria Guevara ◽  
Alessandro Grandini ◽  
Immacolata Maresca ◽  
Matteo Radice ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Pseudomonas Syringae ◽  
Rosmarinic Acid ◽  
Plant Protection ◽  
Biological Activities ◽  
Radical Scavenger ◽  
Radical Scavenger Activity ◽  
Antioxidant Agents ◽  
Scavenger Activity

The essential oil (EO), the methanolic (MeOH), and the 70% ethanolic (70% EtOH) extracts obtained from the aerial parts of Ocimum campechianum Mill. (Ecuador) were chemically characterized through gas-chromatography coupled to mass spectrometry detector (GC-MS), high-performance liquid chromatography coupled to diode array-mass spectrometry detectors (HPLC-DAD-MS) and studied for their in vitro biological activity. The radical scavenger activity, performed by spectrophotometric 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays, highlighted significant IC50 values for the EO, extracts and their main constituents (eugenol and rosmarinic acid). EO (and eugenol) showed noteworthy activity against Pseudomonas syringae pv. syringae and a moderate effect against clinical Candida strains, with possible synergism in association to fluconazole against the latter microorganisms. The extracts and pure molecules exhibited weak cytotoxic activity against the HaCat cell line and no mutagenicity against Salmonella typhimurium TA98 and TA100 strains, giving indication of safety. Instead, EO showed a weak activity against adenocarcinomic human alveolar basal epithelial cells (A549). The above-mentioned evidence leads us to suggest a potential use of the crude drug, extracts, and EO in cosmetic formulation and food supplements as antioxidant agents. In addition, EO may also have a possible application in plant protection and anti-Candida formulations.

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