CD44 Antibody-Conjugated Gold Nanostars as SERS Probes for Distinguishing Cancer Cells (A549 Cells, H1229 Cells) from Normal Cells (ATII Cells)

NANO ◽  
2015 ◽  
Vol 10 (03) ◽  
pp. 1550034 ◽  
Author(s):  
Hang Zhao ◽  
Xiaowei Cao ◽  
Man Wang ◽  
Lin Tao ◽  
Xiaoyu Pan ◽  
...  
Keyword(s):  
Cancer Cells ◽  
A549 Cells ◽  
High Sensitivity ◽  
Surface Enhanced Raman Spectroscopy ◽  
Protective Agent ◽  
Normal Cells ◽  
Gold Nanostars ◽  
Surface Enhanced Raman ◽  
Good Biocompatibility ◽  
Cd44 Antibody

In this paper, we report a novel antibody conjugated gold nanostars ( AuNSs ) as surface-enhanced Raman spectroscopy (SERS) probes for distinguishing cancer cells (A549 cells, H1229 cells) from normal cells (ATII cells). In such a probe, bovine serum albumin (BSA) was served as the protective agent and stabilizing agent, 4-mercaptobenzoic acid (4-MBA) was used as the Raman reporter to generate SERS signals as well as the conjugation agent for attaching CD44 antibody (anti-CD44) molecules to AuNSs , where anti-CD44 could effectively target to CD44 protein over-expressed cells. All the results of the experiments confirmed that more SERS probes have been targeted to cancer cells (A549 cells and H1229 cells) than that of normal cells (ATII cells) under the same condition. The anti-CD44 SERS probes hold a potential application in distinguishing cancer cells from normal cells with high sensitivity and good biocompatibility.

Rapid Detection of Carbapenemase-Producing Enterobacteriacae Based on Surface-Enhanced Raman Spectroscopy with Gold Nanostars

2020 ◽  
Vol 6 (5) ◽  
pp. 947-953 ◽  
Author(s):  
Yen Lynn Wong ◽  
Wei Cherng Malvin Kang ◽  
Miguel Reyes ◽  
Jeanette Woon Pei Teo ◽  
James Chen Yong Kah
Keyword(s):  
Raman Spectroscopy ◽  
Rapid Detection ◽  
Surface Enhanced Raman Spectroscopy ◽  
Gold Nanostars ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

scholarly journals Ultrahigh sensitive Raman spectroscopy for subnanoscience: Direct observation of tin oxide clusters

2019 ◽  
Vol 5 (12) ◽  
pp. eaax6455 ◽  
Author(s):  
Akiyoshi Kuzume ◽  
Miyu Ozawa ◽  
Yuansen Tang ◽  
Yuki Yamada ◽  
Naoki Haruta ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Tin Oxide ◽  
Density Functional ◽  
Rational Design ◽  
High Sensitivity ◽  
Density Functional Theory Calculations ◽  
Surface Enhanced Raman Spectroscopy ◽  
Atomic Scale ◽  
Practical Applications ◽  
Surface Enhanced Raman

Subnanometric metal clusters exhibit anomalous catalytic activity, suggesting innovative applications as next-generation materials, although identifying and characterizing these subnanomaterials in atomic detail remains a substantial challenge because of the severely weak signal intensity for the conventional analytical methods. Here, we report a subnanosensitive vibrational technique established based on the surface-enhanced Raman spectroscopy, demonstrating the first-ever detailed vibrational characterization of subnanomaterials. Furthermore, combining with density functional theory calculations, we reveal that inherent surface structures of the tin oxide subnanoclusters determine the size-specific spectral and catalytic characteristics of these clusters. The high-sensitivity characterization methodology elaborated here can provide a comprehensive understanding of the chemical and structural natures of subnanomaterials, which facilitate the rational design of subnanomaterials on the atomic scale for practical applications, such as in catalysts, biosensors, and electronics.

Uptake of silver nanoparticles by DHA-treated cancer cells examined by surface-enhanced Raman spectroscopy in a microfluidic chip

Lab on a Chip ◽  
2017 ◽  
Vol 17 (7) ◽  
pp. 1306-1313 ◽  
Author(s):  
Zhimin Zhai ◽  
Fengqiu Zhang ◽  
Xiangyu Chen ◽  
Jie Zhong ◽  
Gang Liu ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Silver Nanoparticles ◽  
Cancer Cells ◽  
Microfluidic Chip ◽  
Surface Enhanced Raman Spectroscopy ◽  
Living Cells ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

This paper reports on the synthesis and application of biocompatible and sensitive SERS nanoparticles for the study of uptake of nanoparticles into living cells in a microfluidic chip through surface-enhanced Raman spectroscopy (SERS).

Self-assembled two-dimensional gold nanoparticle film for sensitive nontargeted analysis of food additives with surface-enhanced Raman spectroscopy

The Analyst ◽  
2018 ◽  
Vol 143 (10) ◽  
pp. 2363-2368 ◽  
Author(s):  
Yiping Wu ◽  
Wenfang Yu ◽  
Benhong Yang ◽  
Pan Li
Keyword(s):  
Self Assembly ◽  
Food Additives ◽  
High Sensitivity ◽  
Surface Enhanced Raman Spectroscopy ◽  
Assembly Strategy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Active Substrate ◽  
Nanoparticle Film ◽  
Sers Active Substrate

CTAB-functionalized Au NP film as SERS active substrate prepared by the evaporation-driven self-assembly strategy demonstrated high sensitivity and reproducibility for the detection of different food additives.

scholarly journals Biosensing Using SERS Active Gold Nanostructures

Nanomaterials ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2679
Author(s):  
Gour Mohan Das ◽  
Stefano Managò ◽  
Maria Mangini ◽  
Anna Chiara De De Luca
Keyword(s):  
High Sensitivity ◽  
Surface Enhanced Raman Spectroscopy ◽  
Fingerprint Recognition ◽  
Chemical Enhancement ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Sensor Configuration ◽  
Relevant Factors ◽  
Selection Of

Surface-enhanced Raman spectroscopy (SERS) has become a powerful tool for biosensing applications owing to its fingerprint recognition, high sensitivity, multiplex detection, and biocompatibility. This review provides an overview of the most significant aspects of SERS for biomedical and biosensing applications. We first introduced the mechanisms at the basis of the SERS amplifications: electromagnetic and chemical enhancement. We then illustrated several types of substrates and fabrication methods, with a focus on gold-based nanostructures. We further analyzed the relevant factors for the characterization of the SERS sensor performances, including sensitivity, reproducibility, stability, sensor configuration (direct or indirect), and nanotoxicity. Finally, a representative selection of applications in the biomedical field is provided.

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