Insight into Bile Duct Reaction to Obstruction from a Three-dimensional Perspective Using ex Vivo Phase-Contrast CT

Radiology ◽  
2021 ◽  
pp. 203967
Author(s):  
Wen-Juan Lv ◽  
Xin-Yan Zhao ◽  
Dou-Dou Hu ◽  
Xiao-Hong Xin ◽  
Li-Li Qin ◽  
...  
Keyword(s):  
Bile Duct ◽  
Phase Contrast ◽  
Ex Vivo ◽  
Three Dimensional ◽  
Contrast Ct ◽  
Insight Into

scholarly journals Assessment of intraductal carcinoma in situ (DCIS) using grating-based X-ray phase-contrast CT at conventional X-ray sources: An experimental ex-vivo study

PLoS ONE ◽  
2019 ◽  
Vol 14 (1) ◽  
pp. e0210291 ◽  
Author(s):  
Karin Hellerhoff ◽  
Lorenz Birnbacher ◽  
Anikó Sztrókay-Gaul ◽  
Susanne Grandl ◽  
Sigrid Auweter ◽  
...  
Keyword(s):  
Carcinoma In Situ ◽  
Phase Contrast ◽  
Ex Vivo ◽  
Intraductal Carcinoma ◽  
X Ray ◽  
Contrast Ct ◽  
Ex Vivo Study

scholarly journals A feasibility study of X-ray phase-contrast mammographic tomography at the Imaging and Medical beamline of the Australian Synchrotron

2015 ◽  
Vol 22 (6) ◽  
pp. 1509-1523 ◽  
Author(s):  
Yakov I. Nesterets ◽  
Timur E. Gureyev ◽  
Sheridan C. Mayo ◽  
Andrew W. Stevenson ◽  
Darren Thompson ◽  
...  
Keyword(s):  
Phase Contrast ◽  
Three Dimensional ◽  
High Sensitivity ◽  
Contrast Imaging ◽  
Detector Resolution ◽  
X Ray ◽  
Processing Strategies ◽  
Characteristics Analysis ◽  
Tissue Characteristics ◽  
Contrast Ct

Results are presented of a recent experiment at the Imaging and Medical beamline of the Australian Synchrotron intended to contribute to the implementation of low-dose high-sensitivity three-dimensional mammographic phase-contrast imaging, initially at synchrotrons and subsequently in hospitals and medical imaging clinics. The effect of such imaging parameters as X-ray energy, source size, detector resolution, sample-to-detector distance, scanning and data processing strategies in the case of propagation-based phase-contrast computed tomography (CT) have been tested, quantified, evaluated and optimized using a plastic phantom simulating relevant breast-tissue characteristics. Analysis of the data collected using a Hamamatsu CMOS Flat Panel Sensor, with a pixel size of 100 µm, revealed the presence of propagation-based phase contrast and demonstrated significant improvement of the quality of phase-contrast CT imaging compared with conventional (absorption-based) CT, at medically acceptable radiation doses.

scholarly journals Amyloid-β plaque deposition measured using propagation-based X-ray phase contrast CT imaging

2016 ◽  
Vol 23 (3) ◽  
pp. 813-819 ◽  
Author(s):  
Alberto Astolfo ◽  
Aurélien Lathuilière ◽  
Vanessa Laversenne ◽  
Bernard Schneider ◽  
Marco Stampanoni
Keyword(s):  
Spatial Resolution ◽  
Phase Contrast ◽  
Ex Vivo ◽  
Three Dimensional ◽  
Amyloid Β ◽  
High Sensitivity ◽  
Final Diagnosis ◽  
Pathological Process ◽  
Imaging Method ◽  
X Ray

Amyloid beta accumulation into insoluble plaques (Aβp) is known to play a significant role in the pathological process in Alzheimer's disease (AD). The presence of Aβp is also one of the neuropathological hallmarks for the disease. AD final diagnosis is generally acknowledged after the evaluation of Aβp deposition in the brain. Insoluble Aβp accumulation may also concur to cause AD as postulated in the so-calledamyloid hypothesis. Therefore, the visualization, evaluation and quantification of Aβp are nowadays the keys for a better understanding of the disease, which may point to a possible cure for AD in the near future. Synchrotron-based X-ray phase contrast (XPC) has been demonstrated as the only imaging method that can retrieve the Aβp signal with high spatial resolution (up to 10 µm), high sensitivity and three-dimensional information at the same time. Although at the moment XPC is suitable forex vivosamples only, it may develop into an alternative to positron emission tomography and magnetic resonance imaging in Aβp imaging. In this contribution the possibility of using synchrotron-based X-ray phase propagation computed tomography to visualize and measure Aβp on mouse brains is presented. A careful setup optimization for this application leads to a significant improvement of spatial resolution (∼1 µm), data acquisition speed (five times faster), X-ray dose (five times lower) and setup complexity, without a substantial loss in sensitivity when compared with the classic implementation of grating-based X-ray interferometry.

Assessment of grating-based X-ray phase-contrast CT for differentiation of invasive ductal carcinoma and ductal carcinoma in situ in an experimental ex vivo set-up

2012 ◽  
Vol 23 (2) ◽  
pp. 381-387 ◽  
Author(s):  
Anikó Sztrókay ◽  
Julia Herzen ◽  
Sigrid D. Auweter ◽  
Susanne Liebhardt ◽  
Doris Mayr ◽  
...  
Keyword(s):  
Invasive Ductal Carcinoma ◽  
Ductal Carcinoma In Situ ◽  
Carcinoma In Situ ◽  
Phase Contrast ◽  
Ductal Carcinoma ◽  
Ex Vivo ◽  
X Ray ◽  
Set Up ◽  
Contrast Ct

scholarly journals Visualizing Typical Features of Breast Fibroadenomas Using Phase-Contrast CT: An Ex-Vivo Study

PLoS ONE ◽  
2014 ◽  
Vol 9 (5) ◽  
pp. e97101 ◽  
Author(s):  
Susanne Grandl ◽  
Marian Willner ◽  
Julia Herzen ◽  
Anikó Sztrókay-Gaul ◽  
Doris Mayr ◽  
...  
Keyword(s):  
Phase Contrast ◽  
Ex Vivo ◽  
Contrast Ct ◽  
Ex Vivo Study

scholarly journals Three-Dimensional Assessment of Bronchiectasis in a Mouse Model of Mucociliary Clearance Disorder

2021 ◽  
pp. 00635-2020
Author(s):  
Willi Wagner ◽  
Christian Dullin ◽  
Stefan Andreas ◽  
Muriel Lizé
Keyword(s):  
Phase Contrast ◽  
Mucociliary Clearance ◽  
Respiratory Infections ◽  
Three Dimensional ◽  
Treatment Strategies ◽  
Ct Imaging ◽  
Murine Models ◽  
Novel Treatment ◽  
Contrast Ct ◽  
Free Propagation

Bronchiectasis is a chronic pulmonary disease affecting the conducting air ways of the lung, which may result from congenital disorders that affect ciliary motility. The disease is being recognised with increasing frequency around the world. Patients with bronchiectasis show permanent enlargement of peripheral bronchi accompanied by repeated respiratory infections, disabling productive cough and shortness of breath, resulting in loss of lung function. Mouse models of reduced mucociliary clearance have failed to display signs of bronchiectasis in multiple studies, hindering the development of targeted therapies. In this brief report we present the detection and quantification of bronchiectasis in TAp73 knockout mice using synchrotron radiation-based free-propagation phase contrast CT imaging, allowing the study of bronchiectasis in a pre-clinical and translational setting for the assessment of novel treatment strategies.Clinical Relevance: Murine models of reduced mucociliary clearance have been established to investigate their pathobiology and develop novel treatment approaches but failed to develop visible airway enlargements. Synchrotron free-propagation phase contrast CT imaging is an innovative, sensitive, non-destructive in-situ technique, that allows for three-dimensional ultra-high-resolution detection of bronchiectasis in murine models of impaired mucociliary clearance.

scholarly journals Liver ductal organoids reconstruct intrahepatic biliary trees in decellularized liver grafts.

2021 ◽  
Author(s):  
Katsuhiro Tomofuji ◽  
Ken Fukumitsu ◽  
Jumpei Kondo ◽  
Hiroshi Horie ◽  
Kenta Makino ◽  
...  
Keyword(s):  
Bile Duct ◽  
Bile Ducts ◽  
Ex Vivo ◽  
Three Dimensional ◽  
Perfusion Culture ◽  
Biliary Tree ◽  
Liver Graft ◽  
Cell Distribution ◽  
Intrahepatic Bile Ducts ◽  
Decellularized Liver

Three-dimensional scaffolds decellularized from native organs are a promising technique to establish engineered liver grafts and overcome the current shortage of donor organs. However, limited sources of bile duct cells and inappropriate cell distribution in bioengineered liver grafts have hindered their practical application. Organoid technology is anticipated to be an excellent tool for the advancement of regenerative medicine. In the present study, we reconstructed intrahepatic bile ducts in a rat decellularized liver graft by recellularization with liver ductal organoids. Using an ex vivo perfusion culture system, we demonstrated the biliary characteristics of repopulated mouse liver organoids, which maintained bile duct markers and reconstructed biliary tree-like networks with luminal structures. We also established a method for the co-recellularization with engineered bile ducts and primary hepatocytes, revealing the appropriate cell distribution to mimic the native liver. We then utilized this model in human organoids to demonstrate the reconstructed bile ducts. Our results show that liver ductal organoids are a potential cell source for bile ducts from bioengineered liver grafts using three-dimensional scaffolds.

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