Mechanism of increased erythrocyte membrane fluidity during magnesium deficiency in weanling rats

1989 ◽  
Vol 257 (2) ◽  
pp. C270-C276 ◽  
Author(s):  
S. Tongyai ◽  
Y. Rayssiguier ◽  
C. Motta ◽  
E. Gueux ◽  
P. Maurois ◽  
...  
Keyword(s):  
Membrane Fluidity ◽  
Erythrocyte Membrane ◽  
Magnesium Deficiency ◽  
Osmotic Fragility ◽  
Magnesium Content ◽  
Male Rats ◽  
Surface Irregularities ◽  
Erythrocyte Membrane Fluidity ◽  
And Control

The erythrocyte membrane was investigated in weanling male rats pair fed with magnesium-deficient and control diets for 8 days. Fluorescence polarization studies revealed a 15% increase in the fluidity of membranes from deficient rats. A similar increase in the fluidity of liposomes indicated that protein was not involved. The change was associated with decreased osmotic fragility of intact erythrocytes; the cells lost their biconcavity and had a flattened appearance with surface irregularities. Analysis of the membranes showed decreased amounts of magnesium, cholesterol, and sphingomyelin in the deficient group. The reduced ratios of cholesterol to phospholipid and sphingomyelin to phosphatidylcholine were consistent with the increased fluidity. Addition of physiological amounts of magnesium to the medium rigidified membranes incubated in tris(hydroxymethyl)-aminomethane buffer, and this was prevented by the presence of EDTA. Cross-incubation experiments with erythrocyte ghosts and plasma from the two groups of rats showed that magnesium-deficient plasma increased the fluidity of control ghosts and control plasma rigidified ghosts from magnesium-deficient rats. Addition of sufficient magnesium chloride to raise the magnesium content of deficient plasma to normal had no significant effect. These results show that the increased fluidity of the erythrocyte membrane in magnesium deficiency is due to physicochemical exchange with the plasma. Although magnesium can directly influence membrane fluidity, the change during its deficiency in vivo is mainly mediated indirectly via disturbances in lipid metabolism.

Action of metformin on erythrocyte membrane fluidity in vitro and in vivo

1997 ◽  
Vol 337 (1) ◽  
pp. 103-110 ◽  
Author(s):  
Sylvaine Muller ◽  
Sylvie Denet ◽  
Harilaos Candiloros ◽  
Roger Barrois ◽  
Nicolas Wiernsperger ◽  
...  
Keyword(s):  
Membrane Fluidity ◽  
Erythrocyte Membrane ◽  
Erythrocyte Membrane Fluidity

Alterations in rat erythrocyte membrane due to hexachlorocyclohexane (technical) exposure

1998 ◽  
Vol 17 (11) ◽  
pp. 638-642 ◽  
Author(s):  
Pankaj Bhalla ◽  
Deepa Agrawal
Keyword(s):  
Membrane Fluidity ◽  
Erythrocyte Membrane ◽  
Osmotic Fragility ◽  
Structure And Function ◽  
Membrane Structure And Function ◽  
Hydrophobic Molecule ◽  
Erythrocyte Membrane Fluidity ◽  
Membrane Bound ◽  
And Function ◽  
Membrane Bound Enzymes

1 Hexachlorocyclohexane (HCH), an organochlorine pesticide having hydrophobic molecule is known to act on membranes. HCH mediated alterations in erythrocyte membrane occur through disorganization of the lipid bilayer. Therefore the changes in erythrocyte membrane fluidity, osmotic fragility and certain membrane bound enzymes were studied. Administration of HCH (technical) to rats at 5 mg/kg, orally, 5 days a week for 1, 2 and 3 months caused marked increase in erythrocyte membrane fluidity, osmotic fragility anddecreaseinlevelsofNa+, K+-ATPase, acetylcholinesterase in erythrocytes and glutathione in blood. 2 These changes indicate that HCH adversely affects membrane structure and function.

scholarly journals In vivo shear flow and erythrocyte membrane fluidity in hypertensive patients

1993 ◽  
Vol 36 (5) ◽  
pp. 437-443 ◽  
Author(s):  
K. H. LE QUAN SANG ◽  
J. LEVENSON ◽  
M. PINO ◽  
A. SIMON ◽  
M.-A. DEVYNCK
Keyword(s):  
Shear Flow ◽  
Membrane Fluidity ◽  
Erythrocyte Membrane ◽  
Hypertensive Patients ◽  
Erythrocyte Membrane Fluidity

Erythrocyte membrane fluidity and haemoglobin haemoporphyrin conformation: features revealed in patients with heart failure

2005 ◽  
Vol 11 (4) ◽  
pp. 209-213 ◽  
Author(s):  
O.V. Rodnenkov ◽  
O.G. Luneva ◽  
N.A. Ulyanova ◽  
G.V. Maksimov ◽  
A.B. Rubin ◽  
...  
Keyword(s):  
Heart Failure ◽  
Membrane Fluidity ◽  
Erythrocyte Membrane ◽  
Patients With Heart Failure ◽  
Erythrocyte Membrane Fluidity

scholarly journals In vivo uptake of [14C]leucine by skeletal muscle ribosomes after injury in rats fed two levels of protein

1969 ◽  
Vol 23 (2) ◽  
pp. 271-280 ◽  
Author(s):  
V. R. Young ◽  
P. C. Huang
Keyword(s):  
Skeletal Muscle ◽  
Specific Activity ◽  
Male Rats ◽  
Thigh Muscle ◽  
Left Femur ◽  
The Right ◽  
And Control ◽  
The Relationship ◽  
In Vivo Uptake

1. After 14 days on a diet containing 5 or 25% casein male rats received a fracture of the left femur. Four hours before they were killed the injured and control rats were injected with [1-14C]leucine; the incorporation of radioactivity into an isolated fraction of skeletal muscle ribosomes was studied 6, 12, 24, 48, 72, 96 and 228 h after injury.2. The incorporation of [14C]leucine into the ribosome fraction in right thigh muscles dropped to 40% of control values 72 h after fracture in well-nourished rats and after 96 h with diets containing 5 or 25%, casein.3. The specific activity of the trichloroacetic acid-soluble fraction of muscle from injured rats was equal to or higher than that of the controls during the first 72 h but lower at 96 h.4. These results suggest that a reduced incorporation of amino acids by ribosomes from the right thigh muscle occurred on day 3 after fracture in the group receiving 25% casein but not in the group receiving 5% casein.5. Muscle RNA and DNA concentrations were not affected by the injury.6. The relationship between these findings and the loss of muscle N after injury is discussed.

Erythrocyte membrane fluidity and indices of plasmatic oxidative damage after acute physical exercise in humans

2010 ◽  
Vol 111 (6) ◽  
pp. 1127-1133 ◽  
Author(s):  
C. Berzosa ◽  
E. M. Gómez–Trullén ◽  
E. Piedrafita ◽  
I. Cebrián ◽  
E. Martínez–Ballarín ◽  
...  
Keyword(s):  
Physical Exercise ◽  
Oxidative Damage ◽  
Membrane Fluidity ◽  
Erythrocyte Membrane ◽  
Erythrocyte Membrane Fluidity ◽  
Acute Physical Exercise ◽  
Exercise In Humans

scholarly journals Supplementation of iron alone and combined with vitamins improves haematological status, erythrocyte membrane fluidity and oxidative stress in anaemic pregnant women

2010 ◽  
Vol 104 (11) ◽  
pp. 1655-1661 ◽  
Author(s):  
Ai Guo Ma ◽  
Evert G. Schouten ◽  
Yong Ye Sun ◽  
Fang Yang ◽  
Xiu Xia Han ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Pregnant Women ◽  
Membrane Fluidity ◽  
Erythrocyte Membrane ◽  
Ferrous Sulphate ◽  
Randomised Trial ◽  
Control Group ◽  
Placebo Control ◽  
Group I ◽  
Erythrocyte Membrane Fluidity

Pregnancy is a condition exhibiting increased susceptibility to oxidative stress, and Fe plays a central role in generating harmful oxygen species. The objective of the present study is to investigate the changes in haematological status, oxidative stress and erythrocyte membrane fluidity in anaemic pregnant women after Fe supplementation with and without combined vitamins. The study was a 2 months double-blind, randomised trial. Pregnant women (n 164) were allocated to four groups: group C was the placebo control group; group I was supplemented daily with 60 mg Fe (ferrous sulphate) daily; group IF was supplemented daily with Fe plus 400 μg folic acid; group IM was supplemented daily with Fe plus 2 mg retinol and 1 mg riboflavin, respectively. After the 2-month trial, Hb significantly increased by 15·8, 17·3 and 21·8 g/l, and ferritin by 2·8, 3·6 and 11·0 μg/l, in the I, IF and IM groups compared with placebo. Polarisation (ρ) and microviscosity (η) decreased significantly in other groups compared with placebo, indicating an increase in membrane fluidity. Significant decreases of ρ and η values compared with group C were 0·033 and 0·959 for group I, 0·037 and 1·074 for group IF and 0·064 and 1·865 for group IM, respectively. In addition, significant increases of glutathione peroxidase activities and decreases of malondialdehyde were shown in all treated groups, as well as increases of plasma retinol and urine riboflavin in group IM. The findings show that supplementation with Fe and particularly in combination with vitamins could improve the haematological status as well as oxidative stress and erythrocyte membrane fluidity.

scholarly journals Spherocytic Hemolytic Disease During Magnesium Deprivation in the Rat

Blood ◽  
1971 ◽  
Vol 38 (4) ◽  
pp. 468-478 ◽  
Author(s):  
MARTIN M. OKEN ◽  
MARSHALL A. LICHTMAN ◽  
DENIS R. MILLER ◽  
PIERRE LEBLOND
Keyword(s):  
Magnesium Deficiency ◽  
Lactate Production ◽  
Male Rats ◽  
Magnesium Concentration ◽  
Red Cell ◽  
Cell Deformability ◽  
Red Cell Deformability ◽  
Intracellular Magnesium ◽  
Erythroid Hyperplasia

Abstract Young male rats (115 g) were maintained on diets containing 4-8 mg of magnesium per 100 g of diet for 12 wk. By 3 wk the characteristic features of magnesium deprivation developed, including decreased plasma and tissue magnesium concentration, growth retardation, ruffled fur, patchy dermatitis, irritability, hyperemia of acral parts, onychymegaly, and in the most severely restricted, premature death. By 7 wk of deprivation, evidence of a hemolytic state existed and thereafter reticulocytosis, spherocytosis, shortened 51Cr red cell survival, erythroid hyperplasia of the bone marrow, and mild anemia were present. Erythrocytes during magnesium deficiency were characterized by decreased intracellular magnesium, glucose utilization, lactate production, ATP and 2,3-DPG concentration. A progressive decrease in red cell deformability as measured by cell elastimetry occurred. The reduction in lactate production and in ATP concentration due to magnesium deficiency may be causal in the development of rigid spherocytes with shortened survival in vivo. In addition, the shape and deformability alteration of the red cell may be due to defective membrane construction in a magnesium-deficient environment.

Sign in / Sign up

Export Citation Format

Share Document