scholarly journals A novel method for quantitative myocardial contrast echocardiography in mice

2018 ◽  
Vol 314 (2) ◽  
pp. H370-H379 ◽  
Author(s):  
E. Alvarez ◽  
N. D. Dalton ◽  
Y. Gu ◽  
D. Smith ◽  
A. Luong ◽  
...  

The small size of the mouse heart frequently imparts technical challenges when applying conventional in vivo imaging methods for assessing heart function. Here, we describe the use of high-frequency ultrasound imaging in conjunction with a size-tuned blood pool contrast agent for quantitatively assessing myocardial perfusion in living mice. A perflurocarbon microbubble formulation exhibiting a narrow size distribution was developed, and echogenicity was assessed at 18 MHz in vitro. Adult mice were subjected to permanent ligation of the left anterior descending artery. Ultrasound imaging was performed on day 7, and a cohort of intact mice was used as a control. Parasternal long-axis cine clips were acquired at 18 MHz before and after contrast administration. Reduced ejection fraction and increased end-systolic volume were observed in infarcted compared with control mice. In control animals, washin of the contrast agent was visible in all myocardial segments. Reduced contrast enhancement was observed in apical-posterolateral regions of all infarcted mice. A novel method for reslicing of the imaging data through the time domain provided a two-dimensional presentation of regional contrast agent washin, enabling convenient identification of locations exhibiting altered perfusion. Myocardial segments exhibiting diminished contractility were observed to have correspondingly low relative myocardial perfusion. The contrast agent formulation and methods demonstrated here provide the basis for simplifying routine in vivo estimation of infarct size in mice and may be particularly useful in longitudinal evaluation of revascularization interventions and assessment of peri-infarct ischemia. NEW & NOTEWORTHY Murine myocardial contrast echocardiography frequently suffers from poor sensitivity to contrast. Here, we formulated a novel size-tuned microbubble contrast agent and validated it for use with ultra-high-frequency ultrasound. A novel data method for evaluating myocardial perfusion based on reslicing the imaging data through the time domain is presented.

2017 ◽  
Vol 37 (suppl_1) ◽  
Author(s):  
Samuel Röhl ◽  
Linnea Eriksson ◽  
Robert Saxelin ◽  
Mariette Lengquist ◽  
Kenneth Caidahl ◽  
...  

Objective: Ultrasound BioMicroscopy (UBM), or high-frequency ultrasound, is a novel technique used for assessment of anatomy and physiology small research animals. In this study, we evaluate the UBM assessment of the re-endothelialization process following denudation of the carotid artery in rats. Methods: Ultrasound BioMicroscopy data from three different experiments were analyzed. A total of 66 rats of three different strains (Sprague-Dawley, Wistar and Goto-Kakizaki) were included in this study. All animals were subjected to common carotid artery balloon injury and examined with UBM 2 and 4 weeks after injury. Re-endothelialization in UBM was measured as the length from the carotid bifurcation to the distal edge of the intimal hyperplasia. En face staining with Evans-blue dye was performed upon euthanization at 4 weeks after injury followed by tissue harvest for morphological and immunohistochemical evaluation. Results: A significant correlation (Spearman r=0.63,p<0.0001) and an agreement according to Bland-Altman test was identified when comparing all measurements of re-endothelialization in high frequency ultrasound and en face staining. Analysis by animal strain revealed a similar pattern and a significant growth in re-endothelialization length measured in UBM from 2 to 4 weeks could be identified. Immunohistochemical staining for von Willebrand factor confirmed the presence of endothelium in the areas detected as re-endothelialized by the ultrasound assessment. Conclusion: Ultrasound BioMicroscopy can be used for longitudinal in vivo assessment of the re-endothelialization following arterial injury in rats.


Author(s):  
A. W. Kastelein ◽  
B. C. de Graaf ◽  
Y. P. Latul ◽  
K. W. J. Verhorstert ◽  
J. Holthof ◽  
...  

Ultrasonics ◽  
2009 ◽  
Vol 49 (2) ◽  
pp. 226-230 ◽  
Author(s):  
Chih-Kuang Yeh ◽  
Jia-Jiun Chen ◽  
Meng-Lin Li ◽  
Jer-Junn Luh ◽  
Jia-Jin Jason Chen

2006 ◽  
Vol 119 (5) ◽  
pp. 3438-3438
Author(s):  
Orlando Aristizábal ◽  
Daniel H. Turnbull ◽  
Jeffrey A. Ketterling

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Kornelia Schuetzenberger ◽  
Martin Pfister ◽  
Alina Messner ◽  
Vanessa Froehlich ◽  
Gerhard Garhoefer ◽  
...  

Abstract Optical coherence tomography (OCT) and high-frequency ultrasound (HFUS), two established imaging modalities in the field of dermatology, were evaluated and compared regarding their applicability for visualization of skin tissue morphology and quantification of murine intradermal structures. The accuracy and reproducibility of both methods were assessed ex vivo and in vivo using a standardized model for intradermal volumes based on injected soft tissue fillers. OCT revealed greater detail in skin morphology, allowing for detection of single layers due to the superior resolution. Volumetric data measured by OCT (7.9 ± 0.3 μl) and HFUS (7.7 ± 0.5 μl) were in good agreement and revealed a high accuracy when compared to the injected volume of 7.98 ± 0.8 µl. In vivo, OCT provided a higher precision (relative SD: 26% OCT vs. 42% HFUS) for the quantification of intradermal structures, whereas HFUS offered increased penetration depth enabling the visualization of deeper structures. A combination of both imaging technologies might be valuable for tumor assessments or other dermal pathologies in clinical settings.


Sensors ◽  
2019 ◽  
Vol 19 (19) ◽  
pp. 4094 ◽  
Author(s):  
Sunmi Yeo ◽  
Changhan Yoon ◽  
Ching-Ling Lien ◽  
Tai-Kyong Song ◽  
K. Kirk Shung

This paper reports the feasibility of Nakagami imaging in monitoring the regeneration process of zebrafish hearts in a noninvasive manner. In addition, spectral Doppler waveforms that are typically used to access the diastolic function were measured to validate the performance of Nakagami imaging. A 30-MHz high-frequency ultrasound array transducer was used to acquire backscattered echo signal for spectral Doppler and Nakagami imaging. The performances of both methods were validated with flow and tissue-mimicking phantom experiments. For in vivo experiments, both spectral Doppler and Nakagami imaging were simultaneously obtained from adult zebrafish with amputated hearts. Longitudinal measurements were performed for five zebrafish. From the experiments, the E/A ratio measured using spectral Doppler imaging increased at 3 days post-amputation (3 dpa) and then decreased to the value before amputation, which were consistent with previous studies. Similar results were obtained from the Nakagami imaging where the Nakagami parameter value increased at 3 dpa and decreased to its original value. These results suggested that the Nakagami and spectral Doppler imaging would be useful techniques in monitoring the regeneration of heart or tissues.


Soft Matter ◽  
2011 ◽  
Vol 7 (9) ◽  
pp. 4341 ◽  
Author(s):  
Anton M. Pavlov ◽  
Veronica Saez ◽  
Andrew Cobley ◽  
John Graves ◽  
Gleb B. Sukhorukov ◽  
...  

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