Accelerator mass spectrometry: application to study of aluminum kinetics in the rat

1991 ◽  
Vol 260 (3) ◽  
pp. F466-F469 ◽  
Author(s):  
O. Meirav ◽  
R. A. Sutton ◽  
D. Fink ◽  
R. Middleton ◽  
J. Klein ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Renal Clearance ◽  
Intravenous Injection ◽  
Accelerator Mass Spectrometry ◽  
Ultrasensitive Detection ◽  
Tracer Studies ◽  
Isotope Tracer ◽  
Tracer Dose ◽  
Normal Rat ◽  
Mass Spectrometry Application

The advent of accelerator mass spectrometry (AMS) now permits the ultrasensitive detection of extremely long-lived isotopes, including 14C, 26Al, and 41Ca. Until now, tracer studies of aluminum kinetics have not been possible because aluminum has only two isotopes, with half-lives of 6.5 min (29Al) and 7 x 10(5) yr (26Al), neither of which is suitable for conventional studies. In a novel experiment we have employed AMS to study aluminum kinetics in a normal rat and a 5/6-nephrectomized rat over a 3-wk period of intravenous injection of a tracer dose of 26Al. Kinetics were similar in the two animals; approximately 75% of intravenously injected tracer 26Al was excreted in the urine in the first 24 h as was approximately 80% after 3 wk. Renal clearance of 26Al was approximately 0.75 ml.min-1.kg body wt-1 in both rats. The results clearly demonstrate the potential of this technique for isotope tracer studies in animals as well as in humans.

scholarly journals Radiocarbon with Gas Chromatography

Radiocarbon ◽  
1995 ◽  
Vol 37 (2) ◽  
pp. 711-716 ◽  
Author(s):  
Christopher Bronk Ramsey ◽  
R. E. M. Hedges
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Pilot Study ◽  
Accelerator Mass Spectrometry ◽  
Ion Source ◽  
Tracer Studies ◽  
Chemical Fractions ◽  
Peak Sensitivity ◽  
Accelerator System

In 14C tracer studies, and when looking for modern contamination in archaeological samples, it is often necessary to measure the 14C concentration of individual chemical fractions. Gas chromatography (GC) is one method that is frequently used for separation of chemical fractions. The gas ion source at the Oxford Radiocarbon Accelerator Unit for accelerator mass spectrometry (AMS) provides the opportunity to measure fractions from a GC instrument directly. Although the first investigations are likely to be 14C tracer studies, such a GC-AMS system could find much wider application. We present results from a pilot study of the peak sensitivity, baseline stability and crosstalk of the accelerator system used in this way. We also discuss the practical considerations in developing a GC-AMS instrument for routine use.

Stable isotope tracer analysis by GC-MS, including quantification of isotopomer effects

1992 ◽  
Vol 263 (3) ◽  
pp. E584-E596 ◽  
Author(s):  
J. Rosenblatt ◽  
D. Chinkes ◽  
M. Wolfe ◽  
R. R. Wolfe
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Stable Isotope ◽  
Gas Chromatography Mass Spectrometry ◽  
Double Vision ◽  
Correction Factors ◽  
Tracer Studies ◽  
Stable Isotope Tracer ◽  
Molar Ratios ◽  
Isotope Tracer

In metabolic tracer studies it is frequently useful to infuse tracers that are differently labeled variants of the same molecule. These tracers are known as isotopomers. Analysis of the enrichment of each isotopic analogue can be accomplished by gas chromatography-mass spectrometry (GC-MS). However, the raw GC-MS data must be corrected to give the information required. This paper addresses how to transform the raw GC-MS data, consisting of relative abundance ratios at specific ion masses, into relative molar ratios of tracer and tracee molecules. Several correction factors are necessary. First, the background must be measured and corrected for, since it is always present in the sample. Second, the abundances in the spectrum of the labeled molecule are different from those in the unlabeled molecule, and this proportionality "skew" is corrected. A third correction factor accounts for the overlapping spectra of two or more isotopomers that cannot be measured independently. The final correction removes the "double vision" effect that may appear in some spectra due to the presence of (M - H)+ species.

Ultrasensitive detection of inhaled organic aerosol particles by accelerator mass spectrometry

Chemosphere ◽  
2016 ◽  
Vol 159 ◽  
pp. 80-88 ◽  
Author(s):  
E.V. Parkhomchuk ◽  
D.G. Gulevich ◽  
A.I. Taratayko ◽  
A.M. Baklanov ◽  
A.V. Selivanova ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Accelerator Mass Spectrometry ◽  
Aerosol Particles ◽  
Organic Aerosol ◽  
Ultrasensitive Detection
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